Antifungal and anti-inflammatory potential of eschweilenol C-rich fraction derived from Terminalia fagifolia Mart
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jep.2019.111941 http://hdl.handle.net/11449/189130 |
Resumo: | Ethnopharmacological Relevance: Folk knowledge transmitted between generations allows traditional populations to maintain the use of medicinal plants for the treatment of several diseases. In this context, the species Terminalia fagifolia Mart., native to Brazil, is used for the treatment of chronic and infectious diseases. Plants rich in secondary metabolites, such as this species and their derivatives, may represent therapeutic alternatives for the treatment of diseases that reduce the quality of life of people. Aim of the study: The aim of this study was to evaluate the antifungal and anti-inflammatory potential of aqueous fraction from ethanolic extract of T. fagifolia, with in silico study of the major compound of the fraction. Material and methods: The phytochemical study of the aqueous fraction was performed by HPLC, LC/MS and NMR. The antifungal activity was evaluated against yeasts, by determination of the minimum inhibitory concentration and minimum fungicidal concentration. The effect on Candida albicans was analyzed by AFM. The antibiofilm potential against biofilms of C. albicans was also tested. The anti-inflammatory potential of the aqueous fraction was evaluated in vivo by the carrageenan-induced paw edema and peritonitis. A microglial model of LPS-induced neuroinflammation was also studied. Further insights on the activation mechanism were studied using quantum chemistry computer simulations. Toxicity was evaluated in the Galleria mellonella and human erythrocytes models. Results: Eschweilenol C was identified as the major constituent of the aqueous fraction of the ethanolic extract of T. fagifolia. The aqueous fraction was active against all Candida strains used (sensitive and resistant to Fluconazole) with MICs ranging from 1000 to 0.4 μg/mL. By AFM it was possible to observe morphological alterations in treated Candida cells. The fraction significantly (p < 0.05) inhibited paw edema and decreased levels of malondialdehyde induced by carrageenan. In a microglial cell model, aqueous fraction demonstrated the ability to inhibit NF-κB after induction with lipopolysaccharide. The theoretical studies showed structural similarity between eschweilenol C and indomethacin and an excellent antioxidant potential. The aqueous fraction did not present toxicity in the studied models. Conclusion: The results indicate that the aqueous fraction of T. fagifolia has potential for biomedical applications with low toxicity. This finding can be attributed to the predominance of eschweilenol C in the aqueous fraction. |
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Antifungal and anti-inflammatory potential of eschweilenol C-rich fraction derived from Terminalia fagifolia MartAcetonitrile (PubChem CID: 6342)AntioxidantCandida sppFormic acid (PubChem CID: 284)Indomethacin (PubChem CID: 3715)InflammationMicroglial cellsTerminaliaTrifluoroacetic acid (PubChem CID: 6422)λ-Carrageenan (PubChem CID: 91972149)Ethnopharmacological Relevance: Folk knowledge transmitted between generations allows traditional populations to maintain the use of medicinal plants for the treatment of several diseases. In this context, the species Terminalia fagifolia Mart., native to Brazil, is used for the treatment of chronic and infectious diseases. Plants rich in secondary metabolites, such as this species and their derivatives, may represent therapeutic alternatives for the treatment of diseases that reduce the quality of life of people. Aim of the study: The aim of this study was to evaluate the antifungal and anti-inflammatory potential of aqueous fraction from ethanolic extract of T. fagifolia, with in silico study of the major compound of the fraction. Material and methods: The phytochemical study of the aqueous fraction was performed by HPLC, LC/MS and NMR. The antifungal activity was evaluated against yeasts, by determination of the minimum inhibitory concentration and minimum fungicidal concentration. The effect on Candida albicans was analyzed by AFM. The antibiofilm potential against biofilms of C. albicans was also tested. The anti-inflammatory potential of the aqueous fraction was evaluated in vivo by the carrageenan-induced paw edema and peritonitis. A microglial model of LPS-induced neuroinflammation was also studied. Further insights on the activation mechanism were studied using quantum chemistry computer simulations. Toxicity was evaluated in the Galleria mellonella and human erythrocytes models. Results: Eschweilenol C was identified as the major constituent of the aqueous fraction of the ethanolic extract of T. fagifolia. The aqueous fraction was active against all Candida strains used (sensitive and resistant to Fluconazole) with MICs ranging from 1000 to 0.4 μg/mL. By AFM it was possible to observe morphological alterations in treated Candida cells. The fraction significantly (p < 0.05) inhibited paw edema and decreased levels of malondialdehyde induced by carrageenan. In a microglial cell model, aqueous fraction demonstrated the ability to inhibit NF-κB after induction with lipopolysaccharide. The theoretical studies showed structural similarity between eschweilenol C and indomethacin and an excellent antioxidant potential. The aqueous fraction did not present toxicity in the studied models. Conclusion: The results indicate that the aqueous fraction of T. fagifolia has potential for biomedical applications with low toxicity. This finding can be attributed to the predominance of eschweilenol C in the aqueous fraction.European Regional Development FundThe Northeast Biotechnology Network RENORBIO Federal University of PiauiBiotechnology and Biodiversity Center Research Biotec Federal University of PiauiLaboratory of Molecular Biology of Dimorphic and Pathogenic Fungi Institute of Biological Sciences University of BrasiliaGlial Cell Biology Laboratory Institute for Research and Innovation in Health i3S University of PortoBioprospectum Lda UPTECFederal Institute of Education Science and Technology of São Paulo Campus MatãoSão Paulo State Universit UNESP Campus of ItapevaMedicinal Plants Research Center NPPM Federal University of PiauiLAQV/REQUIMTE Department of Chemistry and Biochemistry Faculty of Sciences of the University of PortoCenter for Research in Applied Morphology and Immunology NuPMIA University of BrasiliaSão Paulo State Universit UNESP Campus of ItapevaEuropean Regional Development Fund: PT 2020Federal University of PiauiUniversity of BrasiliaUniversity of PortoUPTECScience and Technology of São PauloUniversidade Estadual Paulista (Unesp)Faculty of Sciences of the University of PortoRodrigues de Araújo, AlyneIles, Brunode Melo Nogueira, KerolayneDias, Jhones do NascimentoPlácido, AlexandraRodrigues, ArturAlbuquerque, PatríciaSilva-Pereira, IldineteSocodatto, RenatoPortugal, Camila C.Relvas, João B.Costa Véras, Leiz MariaDalmatti Alves Lima, Filipe CamargoBatagin-Neto, Augusto [UNESP]Rolim Medeiros, Jand-VenesMoreira Nunes, Paulo HumbertoEaton, Peterde Souza de Almeida Leite, José Roberto2019-10-06T16:30:47Z2019-10-06T16:30:47Z2019-08-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jep.2019.111941Journal of Ethnopharmacology, v. 240.1872-75730378-8741http://hdl.handle.net/11449/18913010.1016/j.jep.2019.1119412-s2.0-85065738202Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Ethnopharmacologyinfo:eu-repo/semantics/openAccess2021-10-23T19:23:45Zoai:repositorio.unesp.br:11449/189130Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:02:30.849450Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antifungal and anti-inflammatory potential of eschweilenol C-rich fraction derived from Terminalia fagifolia Mart |
title |
Antifungal and anti-inflammatory potential of eschweilenol C-rich fraction derived from Terminalia fagifolia Mart |
spellingShingle |
Antifungal and anti-inflammatory potential of eschweilenol C-rich fraction derived from Terminalia fagifolia Mart Rodrigues de Araújo, Alyne Acetonitrile (PubChem CID: 6342) Antioxidant Candida spp Formic acid (PubChem CID: 284) Indomethacin (PubChem CID: 3715) Inflammation Microglial cells Terminalia Trifluoroacetic acid (PubChem CID: 6422) λ-Carrageenan (PubChem CID: 91972149) |
title_short |
Antifungal and anti-inflammatory potential of eschweilenol C-rich fraction derived from Terminalia fagifolia Mart |
title_full |
Antifungal and anti-inflammatory potential of eschweilenol C-rich fraction derived from Terminalia fagifolia Mart |
title_fullStr |
Antifungal and anti-inflammatory potential of eschweilenol C-rich fraction derived from Terminalia fagifolia Mart |
title_full_unstemmed |
Antifungal and anti-inflammatory potential of eschweilenol C-rich fraction derived from Terminalia fagifolia Mart |
title_sort |
Antifungal and anti-inflammatory potential of eschweilenol C-rich fraction derived from Terminalia fagifolia Mart |
author |
Rodrigues de Araújo, Alyne |
author_facet |
Rodrigues de Araújo, Alyne Iles, Bruno de Melo Nogueira, Kerolayne Dias, Jhones do Nascimento Plácido, Alexandra Rodrigues, Artur Albuquerque, Patrícia Silva-Pereira, Ildinete Socodatto, Renato Portugal, Camila C. Relvas, João B. Costa Véras, Leiz Maria Dalmatti Alves Lima, Filipe Camargo Batagin-Neto, Augusto [UNESP] Rolim Medeiros, Jand-Venes Moreira Nunes, Paulo Humberto Eaton, Peter de Souza de Almeida Leite, José Roberto |
author_role |
author |
author2 |
Iles, Bruno de Melo Nogueira, Kerolayne Dias, Jhones do Nascimento Plácido, Alexandra Rodrigues, Artur Albuquerque, Patrícia Silva-Pereira, Ildinete Socodatto, Renato Portugal, Camila C. Relvas, João B. Costa Véras, Leiz Maria Dalmatti Alves Lima, Filipe Camargo Batagin-Neto, Augusto [UNESP] Rolim Medeiros, Jand-Venes Moreira Nunes, Paulo Humberto Eaton, Peter de Souza de Almeida Leite, José Roberto |
author2_role |
author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Federal University of Piaui University of Brasilia University of Porto UPTEC Science and Technology of São Paulo Universidade Estadual Paulista (Unesp) Faculty of Sciences of the University of Porto |
dc.contributor.author.fl_str_mv |
Rodrigues de Araújo, Alyne Iles, Bruno de Melo Nogueira, Kerolayne Dias, Jhones do Nascimento Plácido, Alexandra Rodrigues, Artur Albuquerque, Patrícia Silva-Pereira, Ildinete Socodatto, Renato Portugal, Camila C. Relvas, João B. Costa Véras, Leiz Maria Dalmatti Alves Lima, Filipe Camargo Batagin-Neto, Augusto [UNESP] Rolim Medeiros, Jand-Venes Moreira Nunes, Paulo Humberto Eaton, Peter de Souza de Almeida Leite, José Roberto |
dc.subject.por.fl_str_mv |
Acetonitrile (PubChem CID: 6342) Antioxidant Candida spp Formic acid (PubChem CID: 284) Indomethacin (PubChem CID: 3715) Inflammation Microglial cells Terminalia Trifluoroacetic acid (PubChem CID: 6422) λ-Carrageenan (PubChem CID: 91972149) |
topic |
Acetonitrile (PubChem CID: 6342) Antioxidant Candida spp Formic acid (PubChem CID: 284) Indomethacin (PubChem CID: 3715) Inflammation Microglial cells Terminalia Trifluoroacetic acid (PubChem CID: 6422) λ-Carrageenan (PubChem CID: 91972149) |
description |
Ethnopharmacological Relevance: Folk knowledge transmitted between generations allows traditional populations to maintain the use of medicinal plants for the treatment of several diseases. In this context, the species Terminalia fagifolia Mart., native to Brazil, is used for the treatment of chronic and infectious diseases. Plants rich in secondary metabolites, such as this species and their derivatives, may represent therapeutic alternatives for the treatment of diseases that reduce the quality of life of people. Aim of the study: The aim of this study was to evaluate the antifungal and anti-inflammatory potential of aqueous fraction from ethanolic extract of T. fagifolia, with in silico study of the major compound of the fraction. Material and methods: The phytochemical study of the aqueous fraction was performed by HPLC, LC/MS and NMR. The antifungal activity was evaluated against yeasts, by determination of the minimum inhibitory concentration and minimum fungicidal concentration. The effect on Candida albicans was analyzed by AFM. The antibiofilm potential against biofilms of C. albicans was also tested. The anti-inflammatory potential of the aqueous fraction was evaluated in vivo by the carrageenan-induced paw edema and peritonitis. A microglial model of LPS-induced neuroinflammation was also studied. Further insights on the activation mechanism were studied using quantum chemistry computer simulations. Toxicity was evaluated in the Galleria mellonella and human erythrocytes models. Results: Eschweilenol C was identified as the major constituent of the aqueous fraction of the ethanolic extract of T. fagifolia. The aqueous fraction was active against all Candida strains used (sensitive and resistant to Fluconazole) with MICs ranging from 1000 to 0.4 μg/mL. By AFM it was possible to observe morphological alterations in treated Candida cells. The fraction significantly (p < 0.05) inhibited paw edema and decreased levels of malondialdehyde induced by carrageenan. In a microglial cell model, aqueous fraction demonstrated the ability to inhibit NF-κB after induction with lipopolysaccharide. The theoretical studies showed structural similarity between eschweilenol C and indomethacin and an excellent antioxidant potential. The aqueous fraction did not present toxicity in the studied models. Conclusion: The results indicate that the aqueous fraction of T. fagifolia has potential for biomedical applications with low toxicity. This finding can be attributed to the predominance of eschweilenol C in the aqueous fraction. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:30:47Z 2019-10-06T16:30:47Z 2019-08-10 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jep.2019.111941 Journal of Ethnopharmacology, v. 240. 1872-7573 0378-8741 http://hdl.handle.net/11449/189130 10.1016/j.jep.2019.111941 2-s2.0-85065738202 |
url |
http://dx.doi.org/10.1016/j.jep.2019.111941 http://hdl.handle.net/11449/189130 |
identifier_str_mv |
Journal of Ethnopharmacology, v. 240. 1872-7573 0378-8741 10.1016/j.jep.2019.111941 2-s2.0-85065738202 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Ethnopharmacology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129576088371200 |