Characterization of titanium surface coated with epidermal growth factor and its effect on human gingival fibroblasts
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.archoralbio.2019.03.025 http://hdl.handle.net/11449/188932 |
Resumo: | Objectives: Different strategies, such as modifications on the implant abutments surface have been proposed to accelerate and improve the formation of the biological seal (BS). The aim of this study was to characterize a titanium (Ti) surface impregnated with epidermal growth factor (EGF) and to assess its influence on the metabolism and adhesion of oral mucosal cells. Design: Ti discs were coated with EGF (100 nM) conjugated with a fluorophore and analyzed by fluorescence microscopy. The surface roughness analysis (Ra) of the EGF-coated Ti was performed by confocal microscopy. The EGF released in the wet environment was determined at 0, 24, 48 and 72 h by fluorimetric quantification. For assessment of the biological effects of EGF-coated Ti, gingival fibroblasts were seeded (5 × 10 4 cells) onto the substrate coated or not with this growth factor. After 24 h, cell adhesion and viability were evaluated by ANOVA and Tukey tests, α =.05. Results: Immediate release of EGF as well as its incorporation by fibroblasts within 1 h after cells were seeded was observed. EGF-coated Ti discs presented significantly enhance surface roughness. Increased cell viability was observed on the EGF-coated discs. Conclusion: EGF applied to Ti discs stimulated the adhesion and metabolism of gingival fibroblasts and could be considered as an interesting alternative for improving the BS. |
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Characterization of titanium surface coated with epidermal growth factor and its effect on human gingival fibroblastsAbutmentCell survivalEpidermal growth factorFibroblastTitaniumObjectives: Different strategies, such as modifications on the implant abutments surface have been proposed to accelerate and improve the formation of the biological seal (BS). The aim of this study was to characterize a titanium (Ti) surface impregnated with epidermal growth factor (EGF) and to assess its influence on the metabolism and adhesion of oral mucosal cells. Design: Ti discs were coated with EGF (100 nM) conjugated with a fluorophore and analyzed by fluorescence microscopy. The surface roughness analysis (Ra) of the EGF-coated Ti was performed by confocal microscopy. The EGF released in the wet environment was determined at 0, 24, 48 and 72 h by fluorimetric quantification. For assessment of the biological effects of EGF-coated Ti, gingival fibroblasts were seeded (5 × 10 4 cells) onto the substrate coated or not with this growth factor. After 24 h, cell adhesion and viability were evaluated by ANOVA and Tukey tests, α =.05. Results: Immediate release of EGF as well as its incorporation by fibroblasts within 1 h after cells were seeded was observed. EGF-coated Ti discs presented significantly enhance surface roughness. Increased cell viability was observed on the EGF-coated discs. Conclusion: EGF applied to Ti discs stimulated the adhesion and metabolism of gingival fibroblasts and could be considered as an interesting alternative for improving the BS.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Dental Materials and Prosthodontics UNESP – Univ. Estadual Paulista Araraquara School of DentistryDepartment of Dentistry Universidade de Ribeirão Preto UNAERPDepartment of Orthodontics and Pediatric Dentistry UNESP – Univ. Estadual Paulista Araraquara School of DentistryDepartment of Physiology and Pathology UNESP – Univ. Estadual Paulista Araraquara School of Dentistry AraraquaraDepartment of Dental Materials and Prosthodontics UNESP – Univ. Estadual Paulista Araraquara School of DentistryDepartment of Orthodontics and Pediatric Dentistry UNESP – Univ. Estadual Paulista Araraquara School of DentistryDepartment of Physiology and Pathology UNESP – Univ. Estadual Paulista Araraquara School of Dentistry AraraquaraFAPESP: 2015/19364-8CNPq: 442637/2014-4Universidade Estadual Paulista (Unesp)UNAERPPansani, Taisa Nogueira [UNESP]Basso, Fernanda GonçalvesSouza, Isabela dos Reis [UNESP]Hebling, Josimeri [UNESP]de Souza Costa, Carlos Alberto [UNESP]2019-10-06T16:23:48Z2019-10-06T16:23:48Z2019-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article48-54http://dx.doi.org/10.1016/j.archoralbio.2019.03.025Archives of Oral Biology, v. 102, p. 48-54.1879-15060003-9969http://hdl.handle.net/11449/18893210.1016/j.archoralbio.2019.03.0252-s2.0-85063882640Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biologyinfo:eu-repo/semantics/openAccess2024-09-27T14:56:51Zoai:repositorio.unesp.br:11449/188932Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:56:51Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Characterization of titanium surface coated with epidermal growth factor and its effect on human gingival fibroblasts |
title |
Characterization of titanium surface coated with epidermal growth factor and its effect on human gingival fibroblasts |
spellingShingle |
Characterization of titanium surface coated with epidermal growth factor and its effect on human gingival fibroblasts Pansani, Taisa Nogueira [UNESP] Abutment Cell survival Epidermal growth factor Fibroblast Titanium |
title_short |
Characterization of titanium surface coated with epidermal growth factor and its effect on human gingival fibroblasts |
title_full |
Characterization of titanium surface coated with epidermal growth factor and its effect on human gingival fibroblasts |
title_fullStr |
Characterization of titanium surface coated with epidermal growth factor and its effect on human gingival fibroblasts |
title_full_unstemmed |
Characterization of titanium surface coated with epidermal growth factor and its effect on human gingival fibroblasts |
title_sort |
Characterization of titanium surface coated with epidermal growth factor and its effect on human gingival fibroblasts |
author |
Pansani, Taisa Nogueira [UNESP] |
author_facet |
Pansani, Taisa Nogueira [UNESP] Basso, Fernanda Gonçalves Souza, Isabela dos Reis [UNESP] Hebling, Josimeri [UNESP] de Souza Costa, Carlos Alberto [UNESP] |
author_role |
author |
author2 |
Basso, Fernanda Gonçalves Souza, Isabela dos Reis [UNESP] Hebling, Josimeri [UNESP] de Souza Costa, Carlos Alberto [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) UNAERP |
dc.contributor.author.fl_str_mv |
Pansani, Taisa Nogueira [UNESP] Basso, Fernanda Gonçalves Souza, Isabela dos Reis [UNESP] Hebling, Josimeri [UNESP] de Souza Costa, Carlos Alberto [UNESP] |
dc.subject.por.fl_str_mv |
Abutment Cell survival Epidermal growth factor Fibroblast Titanium |
topic |
Abutment Cell survival Epidermal growth factor Fibroblast Titanium |
description |
Objectives: Different strategies, such as modifications on the implant abutments surface have been proposed to accelerate and improve the formation of the biological seal (BS). The aim of this study was to characterize a titanium (Ti) surface impregnated with epidermal growth factor (EGF) and to assess its influence on the metabolism and adhesion of oral mucosal cells. Design: Ti discs were coated with EGF (100 nM) conjugated with a fluorophore and analyzed by fluorescence microscopy. The surface roughness analysis (Ra) of the EGF-coated Ti was performed by confocal microscopy. The EGF released in the wet environment was determined at 0, 24, 48 and 72 h by fluorimetric quantification. For assessment of the biological effects of EGF-coated Ti, gingival fibroblasts were seeded (5 × 10 4 cells) onto the substrate coated or not with this growth factor. After 24 h, cell adhesion and viability were evaluated by ANOVA and Tukey tests, α =.05. Results: Immediate release of EGF as well as its incorporation by fibroblasts within 1 h after cells were seeded was observed. EGF-coated Ti discs presented significantly enhance surface roughness. Increased cell viability was observed on the EGF-coated discs. Conclusion: EGF applied to Ti discs stimulated the adhesion and metabolism of gingival fibroblasts and could be considered as an interesting alternative for improving the BS. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:23:48Z 2019-10-06T16:23:48Z 2019-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.archoralbio.2019.03.025 Archives of Oral Biology, v. 102, p. 48-54. 1879-1506 0003-9969 http://hdl.handle.net/11449/188932 10.1016/j.archoralbio.2019.03.025 2-s2.0-85063882640 |
url |
http://dx.doi.org/10.1016/j.archoralbio.2019.03.025 http://hdl.handle.net/11449/188932 |
identifier_str_mv |
Archives of Oral Biology, v. 102, p. 48-54. 1879-1506 0003-9969 10.1016/j.archoralbio.2019.03.025 2-s2.0-85063882640 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Oral Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
48-54 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1813546436910907392 |