Characterization of titanium surface coated with epidermal growth factor and its effect on human gingival fibroblasts

Detalhes bibliográficos
Autor(a) principal: Pansani, Taisa Nogueira [UNESP]
Data de Publicação: 2019
Outros Autores: Basso, Fernanda Gonçalves, Souza, Isabela dos Reis [UNESP], Hebling, Josimeri [UNESP], de Souza Costa, Carlos Alberto [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.archoralbio.2019.03.025
http://hdl.handle.net/11449/188932
Resumo: Objectives: Different strategies, such as modifications on the implant abutments surface have been proposed to accelerate and improve the formation of the biological seal (BS). The aim of this study was to characterize a titanium (Ti) surface impregnated with epidermal growth factor (EGF) and to assess its influence on the metabolism and adhesion of oral mucosal cells. Design: Ti discs were coated with EGF (100 nM) conjugated with a fluorophore and analyzed by fluorescence microscopy. The surface roughness analysis (Ra) of the EGF-coated Ti was performed by confocal microscopy. The EGF released in the wet environment was determined at 0, 24, 48 and 72 h by fluorimetric quantification. For assessment of the biological effects of EGF-coated Ti, gingival fibroblasts were seeded (5 × 10 4 cells) onto the substrate coated or not with this growth factor. After 24 h, cell adhesion and viability were evaluated by ANOVA and Tukey tests, α =.05. Results: Immediate release of EGF as well as its incorporation by fibroblasts within 1 h after cells were seeded was observed. EGF-coated Ti discs presented significantly enhance surface roughness. Increased cell viability was observed on the EGF-coated discs. Conclusion: EGF applied to Ti discs stimulated the adhesion and metabolism of gingival fibroblasts and could be considered as an interesting alternative for improving the BS.
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spelling Characterization of titanium surface coated with epidermal growth factor and its effect on human gingival fibroblastsAbutmentCell survivalEpidermal growth factorFibroblastTitaniumObjectives: Different strategies, such as modifications on the implant abutments surface have been proposed to accelerate and improve the formation of the biological seal (BS). The aim of this study was to characterize a titanium (Ti) surface impregnated with epidermal growth factor (EGF) and to assess its influence on the metabolism and adhesion of oral mucosal cells. Design: Ti discs were coated with EGF (100 nM) conjugated with a fluorophore and analyzed by fluorescence microscopy. The surface roughness analysis (Ra) of the EGF-coated Ti was performed by confocal microscopy. The EGF released in the wet environment was determined at 0, 24, 48 and 72 h by fluorimetric quantification. For assessment of the biological effects of EGF-coated Ti, gingival fibroblasts were seeded (5 × 10 4 cells) onto the substrate coated or not with this growth factor. After 24 h, cell adhesion and viability were evaluated by ANOVA and Tukey tests, α =.05. Results: Immediate release of EGF as well as its incorporation by fibroblasts within 1 h after cells were seeded was observed. EGF-coated Ti discs presented significantly enhance surface roughness. Increased cell viability was observed on the EGF-coated discs. Conclusion: EGF applied to Ti discs stimulated the adhesion and metabolism of gingival fibroblasts and could be considered as an interesting alternative for improving the BS.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Dental Materials and Prosthodontics UNESP – Univ. Estadual Paulista Araraquara School of DentistryDepartment of Dentistry Universidade de Ribeirão Preto UNAERPDepartment of Orthodontics and Pediatric Dentistry UNESP – Univ. Estadual Paulista Araraquara School of DentistryDepartment of Physiology and Pathology UNESP – Univ. Estadual Paulista Araraquara School of Dentistry AraraquaraDepartment of Dental Materials and Prosthodontics UNESP – Univ. Estadual Paulista Araraquara School of DentistryDepartment of Orthodontics and Pediatric Dentistry UNESP – Univ. Estadual Paulista Araraquara School of DentistryDepartment of Physiology and Pathology UNESP – Univ. Estadual Paulista Araraquara School of Dentistry AraraquaraFAPESP: 2015/19364-8CNPq: 442637/2014-4Universidade Estadual Paulista (Unesp)UNAERPPansani, Taisa Nogueira [UNESP]Basso, Fernanda GonçalvesSouza, Isabela dos Reis [UNESP]Hebling, Josimeri [UNESP]de Souza Costa, Carlos Alberto [UNESP]2019-10-06T16:23:48Z2019-10-06T16:23:48Z2019-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article48-54http://dx.doi.org/10.1016/j.archoralbio.2019.03.025Archives of Oral Biology, v. 102, p. 48-54.1879-15060003-9969http://hdl.handle.net/11449/18893210.1016/j.archoralbio.2019.03.0252-s2.0-85063882640Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biologyinfo:eu-repo/semantics/openAccess2024-09-27T14:56:51Zoai:repositorio.unesp.br:11449/188932Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:56:51Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Characterization of titanium surface coated with epidermal growth factor and its effect on human gingival fibroblasts
title Characterization of titanium surface coated with epidermal growth factor and its effect on human gingival fibroblasts
spellingShingle Characterization of titanium surface coated with epidermal growth factor and its effect on human gingival fibroblasts
Pansani, Taisa Nogueira [UNESP]
Abutment
Cell survival
Epidermal growth factor
Fibroblast
Titanium
title_short Characterization of titanium surface coated with epidermal growth factor and its effect on human gingival fibroblasts
title_full Characterization of titanium surface coated with epidermal growth factor and its effect on human gingival fibroblasts
title_fullStr Characterization of titanium surface coated with epidermal growth factor and its effect on human gingival fibroblasts
title_full_unstemmed Characterization of titanium surface coated with epidermal growth factor and its effect on human gingival fibroblasts
title_sort Characterization of titanium surface coated with epidermal growth factor and its effect on human gingival fibroblasts
author Pansani, Taisa Nogueira [UNESP]
author_facet Pansani, Taisa Nogueira [UNESP]
Basso, Fernanda Gonçalves
Souza, Isabela dos Reis [UNESP]
Hebling, Josimeri [UNESP]
de Souza Costa, Carlos Alberto [UNESP]
author_role author
author2 Basso, Fernanda Gonçalves
Souza, Isabela dos Reis [UNESP]
Hebling, Josimeri [UNESP]
de Souza Costa, Carlos Alberto [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
UNAERP
dc.contributor.author.fl_str_mv Pansani, Taisa Nogueira [UNESP]
Basso, Fernanda Gonçalves
Souza, Isabela dos Reis [UNESP]
Hebling, Josimeri [UNESP]
de Souza Costa, Carlos Alberto [UNESP]
dc.subject.por.fl_str_mv Abutment
Cell survival
Epidermal growth factor
Fibroblast
Titanium
topic Abutment
Cell survival
Epidermal growth factor
Fibroblast
Titanium
description Objectives: Different strategies, such as modifications on the implant abutments surface have been proposed to accelerate and improve the formation of the biological seal (BS). The aim of this study was to characterize a titanium (Ti) surface impregnated with epidermal growth factor (EGF) and to assess its influence on the metabolism and adhesion of oral mucosal cells. Design: Ti discs were coated with EGF (100 nM) conjugated with a fluorophore and analyzed by fluorescence microscopy. The surface roughness analysis (Ra) of the EGF-coated Ti was performed by confocal microscopy. The EGF released in the wet environment was determined at 0, 24, 48 and 72 h by fluorimetric quantification. For assessment of the biological effects of EGF-coated Ti, gingival fibroblasts were seeded (5 × 10 4 cells) onto the substrate coated or not with this growth factor. After 24 h, cell adhesion and viability were evaluated by ANOVA and Tukey tests, α =.05. Results: Immediate release of EGF as well as its incorporation by fibroblasts within 1 h after cells were seeded was observed. EGF-coated Ti discs presented significantly enhance surface roughness. Increased cell viability was observed on the EGF-coated discs. Conclusion: EGF applied to Ti discs stimulated the adhesion and metabolism of gingival fibroblasts and could be considered as an interesting alternative for improving the BS.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T16:23:48Z
2019-10-06T16:23:48Z
2019-06-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.archoralbio.2019.03.025
Archives of Oral Biology, v. 102, p. 48-54.
1879-1506
0003-9969
http://hdl.handle.net/11449/188932
10.1016/j.archoralbio.2019.03.025
2-s2.0-85063882640
url http://dx.doi.org/10.1016/j.archoralbio.2019.03.025
http://hdl.handle.net/11449/188932
identifier_str_mv Archives of Oral Biology, v. 102, p. 48-54.
1879-1506
0003-9969
10.1016/j.archoralbio.2019.03.025
2-s2.0-85063882640
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Archives of Oral Biology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 48-54
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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