EGF coating of titanium surfaces modulates cytokines in oral mucosal primary cells exposed to TNF-α
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/jre.13138 http://hdl.handle.net/11449/248869 |
Resumo: | Objective: This study assessed the metabolism of oral mucosal cells cultured on titanium discs (Ti) coated (or not) with epidermal growth factor (EGF) and exposed to tumor necrosis factor alpha (TNF-α). Methods: Fibroblasts or keratinocytes were seeded on Ti coated or not with EGF, and then exposed to 100 ng/mL of TNF-α for 24 h. Groups were established: G1: Ti (control); G2: Ti + TNF-α; G3: Ti + EGF; and G4: Ti + EGF + TNF-α. Both cell lines were evaluated for: viability (AlamarBlue®, n = 8); interleukin 6 and 8 (IL-6, IL-8) gene expression (qPCR, n = 5), and protein synthesis (ELISA, n = 6). For keratinocytes cells, the matrix metalloproteinase type 3 (MMP-3) was evaluated by qPCR (n = 5) and ELISA (n = 6). A 3-D culture of fibroblasts was analyzed by confocal microscopy. The data were subjected to ANOVA analysis, α = 5%. Results: Increased cell viability was observed in all groups compared with G1. Enhanced gene expression and synthesis of IL-6 and IL-8 by fibroblasts and keratinocytes in G2 and modulation of hIL-6 gene expression in G4 was noted. Modulation of IL-8 synthesis occurred in keratinocytes in G3 and G4. Keratinocytes in G2 showed enhanced gene expression of hMMP-3. A 3-D culture showed more cells in G3. Fibroblasts in G2 exhibited disrupted cytoplasmic membrane. Cells in G4 showed elongated morphology with intact cytoplasm. Conclusions: EGF coating increases cell viability and modulates the response of oral cells exposed to an inflammatory stimulus. |
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EGF coating of titanium surfaces modulates cytokines in oral mucosal primary cells exposed to TNF-αepidermal growth factorfibroblastskeratinocytestitaniumtumor necrosis factor alphaObjective: This study assessed the metabolism of oral mucosal cells cultured on titanium discs (Ti) coated (or not) with epidermal growth factor (EGF) and exposed to tumor necrosis factor alpha (TNF-α). Methods: Fibroblasts or keratinocytes were seeded on Ti coated or not with EGF, and then exposed to 100 ng/mL of TNF-α for 24 h. Groups were established: G1: Ti (control); G2: Ti + TNF-α; G3: Ti + EGF; and G4: Ti + EGF + TNF-α. Both cell lines were evaluated for: viability (AlamarBlue®, n = 8); interleukin 6 and 8 (IL-6, IL-8) gene expression (qPCR, n = 5), and protein synthesis (ELISA, n = 6). For keratinocytes cells, the matrix metalloproteinase type 3 (MMP-3) was evaluated by qPCR (n = 5) and ELISA (n = 6). A 3-D culture of fibroblasts was analyzed by confocal microscopy. The data were subjected to ANOVA analysis, α = 5%. Results: Increased cell viability was observed in all groups compared with G1. Enhanced gene expression and synthesis of IL-6 and IL-8 by fibroblasts and keratinocytes in G2 and modulation of hIL-6 gene expression in G4 was noted. Modulation of IL-8 synthesis occurred in keratinocytes in G3 and G4. Keratinocytes in G2 showed enhanced gene expression of hMMP-3. A 3-D culture showed more cells in G3. Fibroblasts in G2 exhibited disrupted cytoplasmic membrane. Cells in G4 showed elongated morphology with intact cytoplasm. Conclusions: EGF coating increases cell viability and modulates the response of oral cells exposed to an inflammatory stimulus.Department of Physiology and Pathology São Paulo State University (UNESP) Araraquara School of DentistryDepartment of Dentistry Universidade de Ribeirão Preto UNAERPDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP) Araraquara School of DentistryDepartment of Physiology and Pathology São Paulo State University (UNESP) Araraquara School of DentistryDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP) Araraquara School of DentistryUniversidade Estadual Paulista (UNESP)UNAERPPansani, Taisa Nogueira [UNESP]Basso, Fernanda GonçalvesCardoso, Laís Medeiros [UNESP]de Souza Costa, Carlos Alberto [UNESP]2023-07-29T13:56:00Z2023-07-29T13:56:00Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1111/jre.13138Journal of Periodontal Research.1600-07650022-3484http://hdl.handle.net/11449/24886910.1111/jre.131382-s2.0-85160043246Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Periodontal Researchinfo:eu-repo/semantics/openAccess2024-09-27T14:56:50Zoai:repositorio.unesp.br:11449/248869Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:56:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
EGF coating of titanium surfaces modulates cytokines in oral mucosal primary cells exposed to TNF-α |
title |
EGF coating of titanium surfaces modulates cytokines in oral mucosal primary cells exposed to TNF-α |
spellingShingle |
EGF coating of titanium surfaces modulates cytokines in oral mucosal primary cells exposed to TNF-α Pansani, Taisa Nogueira [UNESP] epidermal growth factor fibroblasts keratinocytes titanium tumor necrosis factor alpha |
title_short |
EGF coating of titanium surfaces modulates cytokines in oral mucosal primary cells exposed to TNF-α |
title_full |
EGF coating of titanium surfaces modulates cytokines in oral mucosal primary cells exposed to TNF-α |
title_fullStr |
EGF coating of titanium surfaces modulates cytokines in oral mucosal primary cells exposed to TNF-α |
title_full_unstemmed |
EGF coating of titanium surfaces modulates cytokines in oral mucosal primary cells exposed to TNF-α |
title_sort |
EGF coating of titanium surfaces modulates cytokines in oral mucosal primary cells exposed to TNF-α |
author |
Pansani, Taisa Nogueira [UNESP] |
author_facet |
Pansani, Taisa Nogueira [UNESP] Basso, Fernanda Gonçalves Cardoso, Laís Medeiros [UNESP] de Souza Costa, Carlos Alberto [UNESP] |
author_role |
author |
author2 |
Basso, Fernanda Gonçalves Cardoso, Laís Medeiros [UNESP] de Souza Costa, Carlos Alberto [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) UNAERP |
dc.contributor.author.fl_str_mv |
Pansani, Taisa Nogueira [UNESP] Basso, Fernanda Gonçalves Cardoso, Laís Medeiros [UNESP] de Souza Costa, Carlos Alberto [UNESP] |
dc.subject.por.fl_str_mv |
epidermal growth factor fibroblasts keratinocytes titanium tumor necrosis factor alpha |
topic |
epidermal growth factor fibroblasts keratinocytes titanium tumor necrosis factor alpha |
description |
Objective: This study assessed the metabolism of oral mucosal cells cultured on titanium discs (Ti) coated (or not) with epidermal growth factor (EGF) and exposed to tumor necrosis factor alpha (TNF-α). Methods: Fibroblasts or keratinocytes were seeded on Ti coated or not with EGF, and then exposed to 100 ng/mL of TNF-α for 24 h. Groups were established: G1: Ti (control); G2: Ti + TNF-α; G3: Ti + EGF; and G4: Ti + EGF + TNF-α. Both cell lines were evaluated for: viability (AlamarBlue®, n = 8); interleukin 6 and 8 (IL-6, IL-8) gene expression (qPCR, n = 5), and protein synthesis (ELISA, n = 6). For keratinocytes cells, the matrix metalloproteinase type 3 (MMP-3) was evaluated by qPCR (n = 5) and ELISA (n = 6). A 3-D culture of fibroblasts was analyzed by confocal microscopy. The data were subjected to ANOVA analysis, α = 5%. Results: Increased cell viability was observed in all groups compared with G1. Enhanced gene expression and synthesis of IL-6 and IL-8 by fibroblasts and keratinocytes in G2 and modulation of hIL-6 gene expression in G4 was noted. Modulation of IL-8 synthesis occurred in keratinocytes in G3 and G4. Keratinocytes in G2 showed enhanced gene expression of hMMP-3. A 3-D culture showed more cells in G3. Fibroblasts in G2 exhibited disrupted cytoplasmic membrane. Cells in G4 showed elongated morphology with intact cytoplasm. Conclusions: EGF coating increases cell viability and modulates the response of oral cells exposed to an inflammatory stimulus. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:56:00Z 2023-07-29T13:56:00Z 2023-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/jre.13138 Journal of Periodontal Research. 1600-0765 0022-3484 http://hdl.handle.net/11449/248869 10.1111/jre.13138 2-s2.0-85160043246 |
url |
http://dx.doi.org/10.1111/jre.13138 http://hdl.handle.net/11449/248869 |
identifier_str_mv |
Journal of Periodontal Research. 1600-0765 0022-3484 10.1111/jre.13138 2-s2.0-85160043246 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Periodontal Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1813546420621279232 |