EGF coating of titanium surfaces modulates cytokines in oral mucosal primary cells exposed to TNF-α

Detalhes bibliográficos
Autor(a) principal: Pansani, Taisa Nogueira [UNESP]
Data de Publicação: 2023
Outros Autores: Basso, Fernanda Gonçalves, Cardoso, Laís Medeiros [UNESP], de Souza Costa, Carlos Alberto [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1111/jre.13138
http://hdl.handle.net/11449/248869
Resumo: Objective: This study assessed the metabolism of oral mucosal cells cultured on titanium discs (Ti) coated (or not) with epidermal growth factor (EGF) and exposed to tumor necrosis factor alpha (TNF-α). Methods: Fibroblasts or keratinocytes were seeded on Ti coated or not with EGF, and then exposed to 100 ng/mL of TNF-α for 24 h. Groups were established: G1: Ti (control); G2: Ti + TNF-α; G3: Ti + EGF; and G4: Ti + EGF + TNF-α. Both cell lines were evaluated for: viability (AlamarBlue®, n = 8); interleukin 6 and 8 (IL-6, IL-8) gene expression (qPCR, n = 5), and protein synthesis (ELISA, n = 6). For keratinocytes cells, the matrix metalloproteinase type 3 (MMP-3) was evaluated by qPCR (n = 5) and ELISA (n = 6). A 3-D culture of fibroblasts was analyzed by confocal microscopy. The data were subjected to ANOVA analysis, α = 5%. Results: Increased cell viability was observed in all groups compared with G1. Enhanced gene expression and synthesis of IL-6 and IL-8 by fibroblasts and keratinocytes in G2 and modulation of hIL-6 gene expression in G4 was noted. Modulation of IL-8 synthesis occurred in keratinocytes in G3 and G4. Keratinocytes in G2 showed enhanced gene expression of hMMP-3. A 3-D culture showed more cells in G3. Fibroblasts in G2 exhibited disrupted cytoplasmic membrane. Cells in G4 showed elongated morphology with intact cytoplasm. Conclusions: EGF coating increases cell viability and modulates the response of oral cells exposed to an inflammatory stimulus.
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spelling EGF coating of titanium surfaces modulates cytokines in oral mucosal primary cells exposed to TNF-αepidermal growth factorfibroblastskeratinocytestitaniumtumor necrosis factor alphaObjective: This study assessed the metabolism of oral mucosal cells cultured on titanium discs (Ti) coated (or not) with epidermal growth factor (EGF) and exposed to tumor necrosis factor alpha (TNF-α). Methods: Fibroblasts or keratinocytes were seeded on Ti coated or not with EGF, and then exposed to 100 ng/mL of TNF-α for 24 h. Groups were established: G1: Ti (control); G2: Ti + TNF-α; G3: Ti + EGF; and G4: Ti + EGF + TNF-α. Both cell lines were evaluated for: viability (AlamarBlue®, n = 8); interleukin 6 and 8 (IL-6, IL-8) gene expression (qPCR, n = 5), and protein synthesis (ELISA, n = 6). For keratinocytes cells, the matrix metalloproteinase type 3 (MMP-3) was evaluated by qPCR (n = 5) and ELISA (n = 6). A 3-D culture of fibroblasts was analyzed by confocal microscopy. The data were subjected to ANOVA analysis, α = 5%. Results: Increased cell viability was observed in all groups compared with G1. Enhanced gene expression and synthesis of IL-6 and IL-8 by fibroblasts and keratinocytes in G2 and modulation of hIL-6 gene expression in G4 was noted. Modulation of IL-8 synthesis occurred in keratinocytes in G3 and G4. Keratinocytes in G2 showed enhanced gene expression of hMMP-3. A 3-D culture showed more cells in G3. Fibroblasts in G2 exhibited disrupted cytoplasmic membrane. Cells in G4 showed elongated morphology with intact cytoplasm. Conclusions: EGF coating increases cell viability and modulates the response of oral cells exposed to an inflammatory stimulus.Department of Physiology and Pathology São Paulo State University (UNESP) Araraquara School of DentistryDepartment of Dentistry Universidade de Ribeirão Preto UNAERPDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP) Araraquara School of DentistryDepartment of Physiology and Pathology São Paulo State University (UNESP) Araraquara School of DentistryDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP) Araraquara School of DentistryUniversidade Estadual Paulista (UNESP)UNAERPPansani, Taisa Nogueira [UNESP]Basso, Fernanda GonçalvesCardoso, Laís Medeiros [UNESP]de Souza Costa, Carlos Alberto [UNESP]2023-07-29T13:56:00Z2023-07-29T13:56:00Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1111/jre.13138Journal of Periodontal Research.1600-07650022-3484http://hdl.handle.net/11449/24886910.1111/jre.131382-s2.0-85160043246Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Periodontal Researchinfo:eu-repo/semantics/openAccess2024-09-27T14:56:50Zoai:repositorio.unesp.br:11449/248869Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:56:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv EGF coating of titanium surfaces modulates cytokines in oral mucosal primary cells exposed to TNF-α
title EGF coating of titanium surfaces modulates cytokines in oral mucosal primary cells exposed to TNF-α
spellingShingle EGF coating of titanium surfaces modulates cytokines in oral mucosal primary cells exposed to TNF-α
Pansani, Taisa Nogueira [UNESP]
epidermal growth factor
fibroblasts
keratinocytes
titanium
tumor necrosis factor alpha
title_short EGF coating of titanium surfaces modulates cytokines in oral mucosal primary cells exposed to TNF-α
title_full EGF coating of titanium surfaces modulates cytokines in oral mucosal primary cells exposed to TNF-α
title_fullStr EGF coating of titanium surfaces modulates cytokines in oral mucosal primary cells exposed to TNF-α
title_full_unstemmed EGF coating of titanium surfaces modulates cytokines in oral mucosal primary cells exposed to TNF-α
title_sort EGF coating of titanium surfaces modulates cytokines in oral mucosal primary cells exposed to TNF-α
author Pansani, Taisa Nogueira [UNESP]
author_facet Pansani, Taisa Nogueira [UNESP]
Basso, Fernanda Gonçalves
Cardoso, Laís Medeiros [UNESP]
de Souza Costa, Carlos Alberto [UNESP]
author_role author
author2 Basso, Fernanda Gonçalves
Cardoso, Laís Medeiros [UNESP]
de Souza Costa, Carlos Alberto [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
UNAERP
dc.contributor.author.fl_str_mv Pansani, Taisa Nogueira [UNESP]
Basso, Fernanda Gonçalves
Cardoso, Laís Medeiros [UNESP]
de Souza Costa, Carlos Alberto [UNESP]
dc.subject.por.fl_str_mv epidermal growth factor
fibroblasts
keratinocytes
titanium
tumor necrosis factor alpha
topic epidermal growth factor
fibroblasts
keratinocytes
titanium
tumor necrosis factor alpha
description Objective: This study assessed the metabolism of oral mucosal cells cultured on titanium discs (Ti) coated (or not) with epidermal growth factor (EGF) and exposed to tumor necrosis factor alpha (TNF-α). Methods: Fibroblasts or keratinocytes were seeded on Ti coated or not with EGF, and then exposed to 100 ng/mL of TNF-α for 24 h. Groups were established: G1: Ti (control); G2: Ti + TNF-α; G3: Ti + EGF; and G4: Ti + EGF + TNF-α. Both cell lines were evaluated for: viability (AlamarBlue®, n = 8); interleukin 6 and 8 (IL-6, IL-8) gene expression (qPCR, n = 5), and protein synthesis (ELISA, n = 6). For keratinocytes cells, the matrix metalloproteinase type 3 (MMP-3) was evaluated by qPCR (n = 5) and ELISA (n = 6). A 3-D culture of fibroblasts was analyzed by confocal microscopy. The data were subjected to ANOVA analysis, α = 5%. Results: Increased cell viability was observed in all groups compared with G1. Enhanced gene expression and synthesis of IL-6 and IL-8 by fibroblasts and keratinocytes in G2 and modulation of hIL-6 gene expression in G4 was noted. Modulation of IL-8 synthesis occurred in keratinocytes in G3 and G4. Keratinocytes in G2 showed enhanced gene expression of hMMP-3. A 3-D culture showed more cells in G3. Fibroblasts in G2 exhibited disrupted cytoplasmic membrane. Cells in G4 showed elongated morphology with intact cytoplasm. Conclusions: EGF coating increases cell viability and modulates the response of oral cells exposed to an inflammatory stimulus.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T13:56:00Z
2023-07-29T13:56:00Z
2023-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1111/jre.13138
Journal of Periodontal Research.
1600-0765
0022-3484
http://hdl.handle.net/11449/248869
10.1111/jre.13138
2-s2.0-85160043246
url http://dx.doi.org/10.1111/jre.13138
http://hdl.handle.net/11449/248869
identifier_str_mv Journal of Periodontal Research.
1600-0765
0022-3484
10.1111/jre.13138
2-s2.0-85160043246
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Periodontal Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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