Effects of EGF-coated titanium surfaces on adhesion and metabolism of bisphosphonate-treated human keratinocytes and gingival fibroblasts
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00784-021-03880-1 http://hdl.handle.net/11449/207611 |
Resumo: | Objective: To assess the effects of epidermal growth factor (EGF)–coated titanium (Ti) discs on the adhesion and metabolism of keratinocytes and gingival fibroblasts exposed to nitrogen-containing bisphosphonates. Materials and methods: Keratinocytes and fibroblasts were seeded (1 × 105 cells/disc) on Ti discs coated with EGF (100 nM). After 24 h, cells were exposed or not to sodium alendronate (SA) or zoledronic acid (ZA) at different concentrations (0 = control, 0.5, 1, or 5 μM) for 48 h. Cell adhesion to the substrates was evaluated by fluorescence microscopy. Cell viability (alamarBlue, n = 6) and synthesis of vascular endothelial growth factor (VEGF), matrix metalloproteinase-2 (MMP-2), and keratinocytes growth factor (KGF) (ELISA, n = 6) were assessed. Data were statistically analyzed by one-way ANOVA and Tukey tests (α = 0.05). Results: Higher cell adhesion rate was observed when keratinocytes and fibroblasts were seeded onto EGF-coated discs in comparison to uncoated discs. ZA treatment hindered the adhesion of both cell lines on the Ti discs as well as reduced the viability and synthesis of VEGF, KGF and MMP-2 by cells (p < 0.05). SA treatment did not affect cell viability, but interfered negatively on the adhesion and synthesis of EGF and KGF by the cells (p < 0.05). EGF-coated surface increased cell viability and synthesis of growth factors as well as downregulated the synthesis of MMP-2 in comparison to control (p < 0.05). Conclusion: EGF applied on Ti surface improves the biological responses of oral mucosa cells exposed to SA and ZA. Clinical relevance: EGF-coating on titanium may be a suitable strategy to improve oral mucosa cellular events related to biological sealing, especially for patients under bisphosphonate therapy. |
id |
UNSP_b43b1ddb65bfc93506cfdee0da16cde3 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/207611 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Effects of EGF-coated titanium surfaces on adhesion and metabolism of bisphosphonate-treated human keratinocytes and gingival fibroblastsBisphosphonatesEpidermal growth factorFibroblastsKeratinocytesTitaniumObjective: To assess the effects of epidermal growth factor (EGF)–coated titanium (Ti) discs on the adhesion and metabolism of keratinocytes and gingival fibroblasts exposed to nitrogen-containing bisphosphonates. Materials and methods: Keratinocytes and fibroblasts were seeded (1 × 105 cells/disc) on Ti discs coated with EGF (100 nM). After 24 h, cells were exposed or not to sodium alendronate (SA) or zoledronic acid (ZA) at different concentrations (0 = control, 0.5, 1, or 5 μM) for 48 h. Cell adhesion to the substrates was evaluated by fluorescence microscopy. Cell viability (alamarBlue, n = 6) and synthesis of vascular endothelial growth factor (VEGF), matrix metalloproteinase-2 (MMP-2), and keratinocytes growth factor (KGF) (ELISA, n = 6) were assessed. Data were statistically analyzed by one-way ANOVA and Tukey tests (α = 0.05). Results: Higher cell adhesion rate was observed when keratinocytes and fibroblasts were seeded onto EGF-coated discs in comparison to uncoated discs. ZA treatment hindered the adhesion of both cell lines on the Ti discs as well as reduced the viability and synthesis of VEGF, KGF and MMP-2 by cells (p < 0.05). SA treatment did not affect cell viability, but interfered negatively on the adhesion and synthesis of EGF and KGF by the cells (p < 0.05). EGF-coated surface increased cell viability and synthesis of growth factors as well as downregulated the synthesis of MMP-2 in comparison to control (p < 0.05). Conclusion: EGF applied on Ti surface improves the biological responses of oral mucosa cells exposed to SA and ZA. Clinical relevance: EGF-coating on titanium may be a suitable strategy to improve oral mucosa cellular events related to biological sealing, especially for patients under bisphosphonate therapy.Department of Dental Materials and Prosthodontics Araraquara School of Dentistry São Paulo State University (UNESP)Department of Dentistry Universidade de Ribeirão Preto UNAERPDepartment of Physiology and Pathology Araraquara School of Dentistry São Paulo State University (UNESP)Department of Dental Materials and Prosthodontics Araraquara School of Dentistry São Paulo State University (UNESP)Department of Physiology and Pathology Araraquara School of Dentistry São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)UNAERPPansani, Taisa Nogueira [UNESP]Cardoso, Laís Medeiros [UNESP]Augusto, Luisa AmmirabileRibeiro, Isabela Massarode Souza Costa, Carlos Alberto [UNESP]Basso, Fernanda Gonçalves2021-06-25T10:58:05Z2021-06-25T10:58:05Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s00784-021-03880-1Clinical Oral Investigations.1436-37711432-6981http://hdl.handle.net/11449/20761110.1007/s00784-021-03880-12-s2.0-85104301567Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigationsinfo:eu-repo/semantics/openAccess2024-09-27T14:57:08Zoai:repositorio.unesp.br:11449/207611Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:57:08Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of EGF-coated titanium surfaces on adhesion and metabolism of bisphosphonate-treated human keratinocytes and gingival fibroblasts |
title |
Effects of EGF-coated titanium surfaces on adhesion and metabolism of bisphosphonate-treated human keratinocytes and gingival fibroblasts |
spellingShingle |
Effects of EGF-coated titanium surfaces on adhesion and metabolism of bisphosphonate-treated human keratinocytes and gingival fibroblasts Pansani, Taisa Nogueira [UNESP] Bisphosphonates Epidermal growth factor Fibroblasts Keratinocytes Titanium |
title_short |
Effects of EGF-coated titanium surfaces on adhesion and metabolism of bisphosphonate-treated human keratinocytes and gingival fibroblasts |
title_full |
Effects of EGF-coated titanium surfaces on adhesion and metabolism of bisphosphonate-treated human keratinocytes and gingival fibroblasts |
title_fullStr |
Effects of EGF-coated titanium surfaces on adhesion and metabolism of bisphosphonate-treated human keratinocytes and gingival fibroblasts |
title_full_unstemmed |
Effects of EGF-coated titanium surfaces on adhesion and metabolism of bisphosphonate-treated human keratinocytes and gingival fibroblasts |
title_sort |
Effects of EGF-coated titanium surfaces on adhesion and metabolism of bisphosphonate-treated human keratinocytes and gingival fibroblasts |
author |
Pansani, Taisa Nogueira [UNESP] |
author_facet |
Pansani, Taisa Nogueira [UNESP] Cardoso, Laís Medeiros [UNESP] Augusto, Luisa Ammirabile Ribeiro, Isabela Massaro de Souza Costa, Carlos Alberto [UNESP] Basso, Fernanda Gonçalves |
author_role |
author |
author2 |
Cardoso, Laís Medeiros [UNESP] Augusto, Luisa Ammirabile Ribeiro, Isabela Massaro de Souza Costa, Carlos Alberto [UNESP] Basso, Fernanda Gonçalves |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) UNAERP |
dc.contributor.author.fl_str_mv |
Pansani, Taisa Nogueira [UNESP] Cardoso, Laís Medeiros [UNESP] Augusto, Luisa Ammirabile Ribeiro, Isabela Massaro de Souza Costa, Carlos Alberto [UNESP] Basso, Fernanda Gonçalves |
dc.subject.por.fl_str_mv |
Bisphosphonates Epidermal growth factor Fibroblasts Keratinocytes Titanium |
topic |
Bisphosphonates Epidermal growth factor Fibroblasts Keratinocytes Titanium |
description |
Objective: To assess the effects of epidermal growth factor (EGF)–coated titanium (Ti) discs on the adhesion and metabolism of keratinocytes and gingival fibroblasts exposed to nitrogen-containing bisphosphonates. Materials and methods: Keratinocytes and fibroblasts were seeded (1 × 105 cells/disc) on Ti discs coated with EGF (100 nM). After 24 h, cells were exposed or not to sodium alendronate (SA) or zoledronic acid (ZA) at different concentrations (0 = control, 0.5, 1, or 5 μM) for 48 h. Cell adhesion to the substrates was evaluated by fluorescence microscopy. Cell viability (alamarBlue, n = 6) and synthesis of vascular endothelial growth factor (VEGF), matrix metalloproteinase-2 (MMP-2), and keratinocytes growth factor (KGF) (ELISA, n = 6) were assessed. Data were statistically analyzed by one-way ANOVA and Tukey tests (α = 0.05). Results: Higher cell adhesion rate was observed when keratinocytes and fibroblasts were seeded onto EGF-coated discs in comparison to uncoated discs. ZA treatment hindered the adhesion of both cell lines on the Ti discs as well as reduced the viability and synthesis of VEGF, KGF and MMP-2 by cells (p < 0.05). SA treatment did not affect cell viability, but interfered negatively on the adhesion and synthesis of EGF and KGF by the cells (p < 0.05). EGF-coated surface increased cell viability and synthesis of growth factors as well as downregulated the synthesis of MMP-2 in comparison to control (p < 0.05). Conclusion: EGF applied on Ti surface improves the biological responses of oral mucosa cells exposed to SA and ZA. Clinical relevance: EGF-coating on titanium may be a suitable strategy to improve oral mucosa cellular events related to biological sealing, especially for patients under bisphosphonate therapy. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:58:05Z 2021-06-25T10:58:05Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00784-021-03880-1 Clinical Oral Investigations. 1436-3771 1432-6981 http://hdl.handle.net/11449/207611 10.1007/s00784-021-03880-1 2-s2.0-85104301567 |
url |
http://dx.doi.org/10.1007/s00784-021-03880-1 http://hdl.handle.net/11449/207611 |
identifier_str_mv |
Clinical Oral Investigations. 1436-3771 1432-6981 10.1007/s00784-021-03880-1 2-s2.0-85104301567 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Clinical Oral Investigations |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1813546470375161856 |