Effects of EGF-coated titanium surfaces on adhesion and metabolism of bisphosphonate-treated human keratinocytes and gingival fibroblasts

Detalhes bibliográficos
Autor(a) principal: Pansani, Taisa Nogueira [UNESP]
Data de Publicação: 2021
Outros Autores: Cardoso, Laís Medeiros [UNESP], Augusto, Luisa Ammirabile, Ribeiro, Isabela Massaro, de Souza Costa, Carlos Alberto [UNESP], Basso, Fernanda Gonçalves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s00784-021-03880-1
http://hdl.handle.net/11449/207611
Resumo: Objective: To assess the effects of epidermal growth factor (EGF)–coated titanium (Ti) discs on the adhesion and metabolism of keratinocytes and gingival fibroblasts exposed to nitrogen-containing bisphosphonates. Materials and methods: Keratinocytes and fibroblasts were seeded (1 × 105 cells/disc) on Ti discs coated with EGF (100 nM). After 24 h, cells were exposed or not to sodium alendronate (SA) or zoledronic acid (ZA) at different concentrations (0 = control, 0.5, 1, or 5 μM) for 48 h. Cell adhesion to the substrates was evaluated by fluorescence microscopy. Cell viability (alamarBlue, n = 6) and synthesis of vascular endothelial growth factor (VEGF), matrix metalloproteinase-2 (MMP-2), and keratinocytes growth factor (KGF) (ELISA, n = 6) were assessed. Data were statistically analyzed by one-way ANOVA and Tukey tests (α = 0.05). Results: Higher cell adhesion rate was observed when keratinocytes and fibroblasts were seeded onto EGF-coated discs in comparison to uncoated discs. ZA treatment hindered the adhesion of both cell lines on the Ti discs as well as reduced the viability and synthesis of VEGF, KGF and MMP-2 by cells (p < 0.05). SA treatment did not affect cell viability, but interfered negatively on the adhesion and synthesis of EGF and KGF by the cells (p < 0.05). EGF-coated surface increased cell viability and synthesis of growth factors as well as downregulated the synthesis of MMP-2 in comparison to control (p < 0.05). Conclusion: EGF applied on Ti surface improves the biological responses of oral mucosa cells exposed to SA and ZA. Clinical relevance: EGF-coating on titanium may be a suitable strategy to improve oral mucosa cellular events related to biological sealing, especially for patients under bisphosphonate therapy.
id UNSP_b43b1ddb65bfc93506cfdee0da16cde3
oai_identifier_str oai:repositorio.unesp.br:11449/207611
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Effects of EGF-coated titanium surfaces on adhesion and metabolism of bisphosphonate-treated human keratinocytes and gingival fibroblastsBisphosphonatesEpidermal growth factorFibroblastsKeratinocytesTitaniumObjective: To assess the effects of epidermal growth factor (EGF)–coated titanium (Ti) discs on the adhesion and metabolism of keratinocytes and gingival fibroblasts exposed to nitrogen-containing bisphosphonates. Materials and methods: Keratinocytes and fibroblasts were seeded (1 × 105 cells/disc) on Ti discs coated with EGF (100 nM). After 24 h, cells were exposed or not to sodium alendronate (SA) or zoledronic acid (ZA) at different concentrations (0 = control, 0.5, 1, or 5 μM) for 48 h. Cell adhesion to the substrates was evaluated by fluorescence microscopy. Cell viability (alamarBlue, n = 6) and synthesis of vascular endothelial growth factor (VEGF), matrix metalloproteinase-2 (MMP-2), and keratinocytes growth factor (KGF) (ELISA, n = 6) were assessed. Data were statistically analyzed by one-way ANOVA and Tukey tests (α = 0.05). Results: Higher cell adhesion rate was observed when keratinocytes and fibroblasts were seeded onto EGF-coated discs in comparison to uncoated discs. ZA treatment hindered the adhesion of both cell lines on the Ti discs as well as reduced the viability and synthesis of VEGF, KGF and MMP-2 by cells (p < 0.05). SA treatment did not affect cell viability, but interfered negatively on the adhesion and synthesis of EGF and KGF by the cells (p < 0.05). EGF-coated surface increased cell viability and synthesis of growth factors as well as downregulated the synthesis of MMP-2 in comparison to control (p < 0.05). Conclusion: EGF applied on Ti surface improves the biological responses of oral mucosa cells exposed to SA and ZA. Clinical relevance: EGF-coating on titanium may be a suitable strategy to improve oral mucosa cellular events related to biological sealing, especially for patients under bisphosphonate therapy.Department of Dental Materials and Prosthodontics Araraquara School of Dentistry São Paulo State University (UNESP)Department of Dentistry Universidade de Ribeirão Preto UNAERPDepartment of Physiology and Pathology Araraquara School of Dentistry São Paulo State University (UNESP)Department of Dental Materials and Prosthodontics Araraquara School of Dentistry São Paulo State University (UNESP)Department of Physiology and Pathology Araraquara School of Dentistry São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)UNAERPPansani, Taisa Nogueira [UNESP]Cardoso, Laís Medeiros [UNESP]Augusto, Luisa AmmirabileRibeiro, Isabela Massarode Souza Costa, Carlos Alberto [UNESP]Basso, Fernanda Gonçalves2021-06-25T10:58:05Z2021-06-25T10:58:05Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s00784-021-03880-1Clinical Oral Investigations.1436-37711432-6981http://hdl.handle.net/11449/20761110.1007/s00784-021-03880-12-s2.0-85104301567Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigationsinfo:eu-repo/semantics/openAccess2024-09-27T14:57:08Zoai:repositorio.unesp.br:11449/207611Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:57:08Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effects of EGF-coated titanium surfaces on adhesion and metabolism of bisphosphonate-treated human keratinocytes and gingival fibroblasts
title Effects of EGF-coated titanium surfaces on adhesion and metabolism of bisphosphonate-treated human keratinocytes and gingival fibroblasts
spellingShingle Effects of EGF-coated titanium surfaces on adhesion and metabolism of bisphosphonate-treated human keratinocytes and gingival fibroblasts
Pansani, Taisa Nogueira [UNESP]
Bisphosphonates
Epidermal growth factor
Fibroblasts
Keratinocytes
Titanium
title_short Effects of EGF-coated titanium surfaces on adhesion and metabolism of bisphosphonate-treated human keratinocytes and gingival fibroblasts
title_full Effects of EGF-coated titanium surfaces on adhesion and metabolism of bisphosphonate-treated human keratinocytes and gingival fibroblasts
title_fullStr Effects of EGF-coated titanium surfaces on adhesion and metabolism of bisphosphonate-treated human keratinocytes and gingival fibroblasts
title_full_unstemmed Effects of EGF-coated titanium surfaces on adhesion and metabolism of bisphosphonate-treated human keratinocytes and gingival fibroblasts
title_sort Effects of EGF-coated titanium surfaces on adhesion and metabolism of bisphosphonate-treated human keratinocytes and gingival fibroblasts
author Pansani, Taisa Nogueira [UNESP]
author_facet Pansani, Taisa Nogueira [UNESP]
Cardoso, Laís Medeiros [UNESP]
Augusto, Luisa Ammirabile
Ribeiro, Isabela Massaro
de Souza Costa, Carlos Alberto [UNESP]
Basso, Fernanda Gonçalves
author_role author
author2 Cardoso, Laís Medeiros [UNESP]
Augusto, Luisa Ammirabile
Ribeiro, Isabela Massaro
de Souza Costa, Carlos Alberto [UNESP]
Basso, Fernanda Gonçalves
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
UNAERP
dc.contributor.author.fl_str_mv Pansani, Taisa Nogueira [UNESP]
Cardoso, Laís Medeiros [UNESP]
Augusto, Luisa Ammirabile
Ribeiro, Isabela Massaro
de Souza Costa, Carlos Alberto [UNESP]
Basso, Fernanda Gonçalves
dc.subject.por.fl_str_mv Bisphosphonates
Epidermal growth factor
Fibroblasts
Keratinocytes
Titanium
topic Bisphosphonates
Epidermal growth factor
Fibroblasts
Keratinocytes
Titanium
description Objective: To assess the effects of epidermal growth factor (EGF)–coated titanium (Ti) discs on the adhesion and metabolism of keratinocytes and gingival fibroblasts exposed to nitrogen-containing bisphosphonates. Materials and methods: Keratinocytes and fibroblasts were seeded (1 × 105 cells/disc) on Ti discs coated with EGF (100 nM). After 24 h, cells were exposed or not to sodium alendronate (SA) or zoledronic acid (ZA) at different concentrations (0 = control, 0.5, 1, or 5 μM) for 48 h. Cell adhesion to the substrates was evaluated by fluorescence microscopy. Cell viability (alamarBlue, n = 6) and synthesis of vascular endothelial growth factor (VEGF), matrix metalloproteinase-2 (MMP-2), and keratinocytes growth factor (KGF) (ELISA, n = 6) were assessed. Data were statistically analyzed by one-way ANOVA and Tukey tests (α = 0.05). Results: Higher cell adhesion rate was observed when keratinocytes and fibroblasts were seeded onto EGF-coated discs in comparison to uncoated discs. ZA treatment hindered the adhesion of both cell lines on the Ti discs as well as reduced the viability and synthesis of VEGF, KGF and MMP-2 by cells (p < 0.05). SA treatment did not affect cell viability, but interfered negatively on the adhesion and synthesis of EGF and KGF by the cells (p < 0.05). EGF-coated surface increased cell viability and synthesis of growth factors as well as downregulated the synthesis of MMP-2 in comparison to control (p < 0.05). Conclusion: EGF applied on Ti surface improves the biological responses of oral mucosa cells exposed to SA and ZA. Clinical relevance: EGF-coating on titanium may be a suitable strategy to improve oral mucosa cellular events related to biological sealing, especially for patients under bisphosphonate therapy.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T10:58:05Z
2021-06-25T10:58:05Z
2021-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00784-021-03880-1
Clinical Oral Investigations.
1436-3771
1432-6981
http://hdl.handle.net/11449/207611
10.1007/s00784-021-03880-1
2-s2.0-85104301567
url http://dx.doi.org/10.1007/s00784-021-03880-1
http://hdl.handle.net/11449/207611
identifier_str_mv Clinical Oral Investigations.
1436-3771
1432-6981
10.1007/s00784-021-03880-1
2-s2.0-85104301567
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Clinical Oral Investigations
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1813546470375161856