Medication-related osteonecrosis of the jaw. Introduction of a new modified experimental model
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0102-865020160050000003 http://hdl.handle.net/11449/168722 |
Resumo: | PURPOSE: To evaluate a modified experimental model for medication-related osteonecrosis of the jaw (MRONJ) through the upper right central incisor extraction followed by intravenous bisphosphonate administration. METHODS: Forty five rats underwent the upper right central incisor tooth extraction were divided in 2 groups: Group I-experimental group, 30 rats received an intravenous administration protocol of zoledronic acid 35μg/kg into the tail vein every two weeks, totalizing four administrations, during eight weeks of administration, previously the extraction, and Group II-control group, 15 rats didn’t received any medication before extraction. The groups were subdivided in postoperative periods: 14/28/42 days. Clinical analysis and microtomography were performed to verify the presence of osteonecrosis. In addition, descritive histological analysis of hematoxylineosin stained sections was performed to evaluate the presence of osteonecrosis or necrotic foci. RESULTS: Twelve (40%) rats, from experimental group, showed clinical signs of MRONJ (p=0.005), however, all samples showed imaginologic findings like osteolysis and loss of integrity of the cellular walls (p≤0.001). Microscopic evaluation revealed osteonecrosis areas with microbial colonies and inflammatory infiltrate (p≤0.001). In the control group, all animals presented the chronology of a normal wound healing. CONCLUSIONS: The presence of medication-related osteonecrosis of the jaw after maxillary central incisor extraction in rats. This new experimental model may be considered an option for the study of MRONJ. |
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Medication-related osteonecrosis of the jaw. Introduction of a new modified experimental modelBisphosphonate-associated osteonecrosis of the jawRatsPURPOSE: To evaluate a modified experimental model for medication-related osteonecrosis of the jaw (MRONJ) through the upper right central incisor extraction followed by intravenous bisphosphonate administration. METHODS: Forty five rats underwent the upper right central incisor tooth extraction were divided in 2 groups: Group I-experimental group, 30 rats received an intravenous administration protocol of zoledronic acid 35μg/kg into the tail vein every two weeks, totalizing four administrations, during eight weeks of administration, previously the extraction, and Group II-control group, 15 rats didn’t received any medication before extraction. The groups were subdivided in postoperative periods: 14/28/42 days. Clinical analysis and microtomography were performed to verify the presence of osteonecrosis. In addition, descritive histological analysis of hematoxylineosin stained sections was performed to evaluate the presence of osteonecrosis or necrotic foci. RESULTS: Twelve (40%) rats, from experimental group, showed clinical signs of MRONJ (p=0.005), however, all samples showed imaginologic findings like osteolysis and loss of integrity of the cellular walls (p≤0.001). Microscopic evaluation revealed osteonecrosis areas with microbial colonies and inflammatory infiltrate (p≤0.001). In the control group, all animals presented the chronology of a normal wound healing. CONCLUSIONS: The presence of medication-related osteonecrosis of the jaw after maxillary central incisor extraction in rats. This new experimental model may be considered an option for the study of MRONJ.Department of Oral Surgery Universidade Sagrado Coração (USC)Department of Oral Surgery Bauru Dental School Universidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Department of Endodontics Bauru Dental School USPDepartment of Implantology Bauru Dental School USPUniversidade Estadual Paulista (UNESP)Universidade Sagrado Coração (USC)Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Curra, CláudiaCardoso, Camila LopesJúnior, Osny FerreiraCuri, Marcos MartinsMatsumoto, Mariza Akemi [UNESP]Cavenago, Bruno Cavalinidos Santos, Pâmela LetíciaSantiago Júnior, Joel Ferreira2018-12-11T16:42:42Z2018-12-11T16:42:42Z2016-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article308-313application/pdfhttp://dx.doi.org/10.1590/S0102-865020160050000003Acta Cirurgica Brasileira, v. 31, n. 5, p. 308-313, 2016.1678-26740102-8650http://hdl.handle.net/11449/16872210.1590/S0102-865020160050000003S0102-865020160005003082-s2.0-84973896437S0102-86502016000500308.pdf8621510886887389Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Cirurgica Brasileira0,395info:eu-repo/semantics/openAccess2023-11-26T06:09:05Zoai:repositorio.unesp.br:11449/168722Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:44:42.999191Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Medication-related osteonecrosis of the jaw. Introduction of a new modified experimental model |
title |
Medication-related osteonecrosis of the jaw. Introduction of a new modified experimental model |
spellingShingle |
Medication-related osteonecrosis of the jaw. Introduction of a new modified experimental model Curra, Cláudia Bisphosphonate-associated osteonecrosis of the jaw Rats |
title_short |
Medication-related osteonecrosis of the jaw. Introduction of a new modified experimental model |
title_full |
Medication-related osteonecrosis of the jaw. Introduction of a new modified experimental model |
title_fullStr |
Medication-related osteonecrosis of the jaw. Introduction of a new modified experimental model |
title_full_unstemmed |
Medication-related osteonecrosis of the jaw. Introduction of a new modified experimental model |
title_sort |
Medication-related osteonecrosis of the jaw. Introduction of a new modified experimental model |
author |
Curra, Cláudia |
author_facet |
Curra, Cláudia Cardoso, Camila Lopes Júnior, Osny Ferreira Curi, Marcos Martins Matsumoto, Mariza Akemi [UNESP] Cavenago, Bruno Cavalini dos Santos, Pâmela Letícia Santiago Júnior, Joel Ferreira |
author_role |
author |
author2 |
Cardoso, Camila Lopes Júnior, Osny Ferreira Curi, Marcos Martins Matsumoto, Mariza Akemi [UNESP] Cavenago, Bruno Cavalini dos Santos, Pâmela Letícia Santiago Júnior, Joel Ferreira |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Sagrado Coração (USC) Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Curra, Cláudia Cardoso, Camila Lopes Júnior, Osny Ferreira Curi, Marcos Martins Matsumoto, Mariza Akemi [UNESP] Cavenago, Bruno Cavalini dos Santos, Pâmela Letícia Santiago Júnior, Joel Ferreira |
dc.subject.por.fl_str_mv |
Bisphosphonate-associated osteonecrosis of the jaw Rats |
topic |
Bisphosphonate-associated osteonecrosis of the jaw Rats |
description |
PURPOSE: To evaluate a modified experimental model for medication-related osteonecrosis of the jaw (MRONJ) through the upper right central incisor extraction followed by intravenous bisphosphonate administration. METHODS: Forty five rats underwent the upper right central incisor tooth extraction were divided in 2 groups: Group I-experimental group, 30 rats received an intravenous administration protocol of zoledronic acid 35μg/kg into the tail vein every two weeks, totalizing four administrations, during eight weeks of administration, previously the extraction, and Group II-control group, 15 rats didn’t received any medication before extraction. The groups were subdivided in postoperative periods: 14/28/42 days. Clinical analysis and microtomography were performed to verify the presence of osteonecrosis. In addition, descritive histological analysis of hematoxylineosin stained sections was performed to evaluate the presence of osteonecrosis or necrotic foci. RESULTS: Twelve (40%) rats, from experimental group, showed clinical signs of MRONJ (p=0.005), however, all samples showed imaginologic findings like osteolysis and loss of integrity of the cellular walls (p≤0.001). Microscopic evaluation revealed osteonecrosis areas with microbial colonies and inflammatory infiltrate (p≤0.001). In the control group, all animals presented the chronology of a normal wound healing. CONCLUSIONS: The presence of medication-related osteonecrosis of the jaw after maxillary central incisor extraction in rats. This new experimental model may be considered an option for the study of MRONJ. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-05-01 2018-12-11T16:42:42Z 2018-12-11T16:42:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0102-865020160050000003 Acta Cirurgica Brasileira, v. 31, n. 5, p. 308-313, 2016. 1678-2674 0102-8650 http://hdl.handle.net/11449/168722 10.1590/S0102-865020160050000003 S0102-86502016000500308 2-s2.0-84973896437 S0102-86502016000500308.pdf 8621510886887389 |
url |
http://dx.doi.org/10.1590/S0102-865020160050000003 http://hdl.handle.net/11449/168722 |
identifier_str_mv |
Acta Cirurgica Brasileira, v. 31, n. 5, p. 308-313, 2016. 1678-2674 0102-8650 10.1590/S0102-865020160050000003 S0102-86502016000500308 2-s2.0-84973896437 S0102-86502016000500308.pdf 8621510886887389 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Cirurgica Brasileira 0,395 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
308-313 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128972532219904 |