Effects of GnRH and the dual regulatory actions of GnIH in the pituitary explants and brain slices of Astyanax altiparanae males
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.ygcen.2018.08.006 http://hdl.handle.net/11449/176721 |
Resumo: | The pituitary gonadotropins, Fsh (follicle-stimulating hormone) and Lh (luteinizing hormone), regulate testicular development and functions in all vertebrates. At the pituitary, different signaling systems regulate the synthesis and secretion of the gonadotropins, such as the hypothalamic neuropeptides GnRH (gonadotropin-releasing hormone) and GnIH (gonadotropin-inhibitory hormone). While GnRH exerts stimulatory roles, the actions of GnIH remain controversial for many teleost species. Therefore, the aim of this study was to evaluate the in vitro effects of chicken GnRH2 (cGnRH2) and zebrafish GnIH-3 (zGnIH-3) on the male gonadotropin and GnRH system expression using pituitary explants and brain slices from a neotropical species with economical and ecological relevance, Astyanax altiparanae. Our results showed that in males, cGnRH2 increased fshb and lhb mRNA levels in the pituitary explants. Interestingly, zGnIH-3 has no effect on basal gonadotropin expression, however zGnIH-3 decreased the cGnRH2-induced fshb and lhb transcripts in male pituitary explants. In the male brain slices, zGnIH-3 showed stimulatory effects, increasing gnrh2 mRNA levels. Overall, our results suggested that GnIH seems to have dual regulatory actions on gonadotropin and GnRH2 expression of A. altiparanae males. This study provided basic information on endocrine regulation of A. altiparanae reproduction, and the obtained results will expand our knowledge, improving the reproductive management of this economically important freshwater species. |
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Effects of GnRH and the dual regulatory actions of GnIH in the pituitary explants and brain slices of Astyanax altiparanae malesAstyanax altiparanaeFollicle-stimulating hormoneGonadotropin-inhibitory hormoneGonadotropin-releasing hormoneLambari-do-rabo-amareloLuteinizing hormoneThe pituitary gonadotropins, Fsh (follicle-stimulating hormone) and Lh (luteinizing hormone), regulate testicular development and functions in all vertebrates. At the pituitary, different signaling systems regulate the synthesis and secretion of the gonadotropins, such as the hypothalamic neuropeptides GnRH (gonadotropin-releasing hormone) and GnIH (gonadotropin-inhibitory hormone). While GnRH exerts stimulatory roles, the actions of GnIH remain controversial for many teleost species. Therefore, the aim of this study was to evaluate the in vitro effects of chicken GnRH2 (cGnRH2) and zebrafish GnIH-3 (zGnIH-3) on the male gonadotropin and GnRH system expression using pituitary explants and brain slices from a neotropical species with economical and ecological relevance, Astyanax altiparanae. Our results showed that in males, cGnRH2 increased fshb and lhb mRNA levels in the pituitary explants. Interestingly, zGnIH-3 has no effect on basal gonadotropin expression, however zGnIH-3 decreased the cGnRH2-induced fshb and lhb transcripts in male pituitary explants. In the male brain slices, zGnIH-3 showed stimulatory effects, increasing gnrh2 mRNA levels. Overall, our results suggested that GnIH seems to have dual regulatory actions on gonadotropin and GnRH2 expression of A. altiparanae males. This study provided basic information on endocrine regulation of A. altiparanae reproduction, and the obtained results will expand our knowledge, improving the reproductive management of this economically important freshwater species.Aquaculture Center of São Paulo State University (CAUNESP) São Paulo State University (UNESP), Jaboticabal CampusReproductive and Molecular Biology Group Department of Morphology Institute of Biosciences São Paulo State University (UNESP), Botucatu CampusInstitute of Biological Sciences and Health Federal University of Alagoas – A. C., Simões CampusDepartment of Biological Sciences University of CalgaryAquaculture Center of São Paulo State University (CAUNESP) São Paulo State University (UNESP), Jaboticabal CampusReproductive and Molecular Biology Group Department of Morphology Institute of Biosciences São Paulo State University (UNESP), Botucatu CampusUniversidade Estadual Paulista (Unesp)Federal University of Alagoas – A. C.University of CalgaryBranco, Giovana Souza [UNESP]Melo, Aline Gomes [UNESP]Ricci, Juliana M.B. [UNESP]Digmayer, Melanie [UNESP]de Jesus, Lázaro W.O.Habibi, Hamid R.Nóbrega, Rafael Henrique [UNESP]2018-12-11T17:22:12Z2018-12-11T17:22:12Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1016/j.ygcen.2018.08.006General and Comparative Endocrinology.1095-68400016-6480http://hdl.handle.net/11449/17672110.1016/j.ygcen.2018.08.0062-s2.0-850516942692-s2.0-85051694269.pdf05157085852539850000-0001-9796-5076Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGeneral and Comparative Endocrinology1,1011,101info:eu-repo/semantics/openAccess2024-04-09T15:10:36Zoai:repositorio.unesp.br:11449/176721Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:29:51.190499Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of GnRH and the dual regulatory actions of GnIH in the pituitary explants and brain slices of Astyanax altiparanae males |
title |
Effects of GnRH and the dual regulatory actions of GnIH in the pituitary explants and brain slices of Astyanax altiparanae males |
spellingShingle |
Effects of GnRH and the dual regulatory actions of GnIH in the pituitary explants and brain slices of Astyanax altiparanae males Branco, Giovana Souza [UNESP] Astyanax altiparanae Follicle-stimulating hormone Gonadotropin-inhibitory hormone Gonadotropin-releasing hormone Lambari-do-rabo-amarelo Luteinizing hormone |
title_short |
Effects of GnRH and the dual regulatory actions of GnIH in the pituitary explants and brain slices of Astyanax altiparanae males |
title_full |
Effects of GnRH and the dual regulatory actions of GnIH in the pituitary explants and brain slices of Astyanax altiparanae males |
title_fullStr |
Effects of GnRH and the dual regulatory actions of GnIH in the pituitary explants and brain slices of Astyanax altiparanae males |
title_full_unstemmed |
Effects of GnRH and the dual regulatory actions of GnIH in the pituitary explants and brain slices of Astyanax altiparanae males |
title_sort |
Effects of GnRH and the dual regulatory actions of GnIH in the pituitary explants and brain slices of Astyanax altiparanae males |
author |
Branco, Giovana Souza [UNESP] |
author_facet |
Branco, Giovana Souza [UNESP] Melo, Aline Gomes [UNESP] Ricci, Juliana M.B. [UNESP] Digmayer, Melanie [UNESP] de Jesus, Lázaro W.O. Habibi, Hamid R. Nóbrega, Rafael Henrique [UNESP] |
author_role |
author |
author2 |
Melo, Aline Gomes [UNESP] Ricci, Juliana M.B. [UNESP] Digmayer, Melanie [UNESP] de Jesus, Lázaro W.O. Habibi, Hamid R. Nóbrega, Rafael Henrique [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Federal University of Alagoas – A. C. University of Calgary |
dc.contributor.author.fl_str_mv |
Branco, Giovana Souza [UNESP] Melo, Aline Gomes [UNESP] Ricci, Juliana M.B. [UNESP] Digmayer, Melanie [UNESP] de Jesus, Lázaro W.O. Habibi, Hamid R. Nóbrega, Rafael Henrique [UNESP] |
dc.subject.por.fl_str_mv |
Astyanax altiparanae Follicle-stimulating hormone Gonadotropin-inhibitory hormone Gonadotropin-releasing hormone Lambari-do-rabo-amarelo Luteinizing hormone |
topic |
Astyanax altiparanae Follicle-stimulating hormone Gonadotropin-inhibitory hormone Gonadotropin-releasing hormone Lambari-do-rabo-amarelo Luteinizing hormone |
description |
The pituitary gonadotropins, Fsh (follicle-stimulating hormone) and Lh (luteinizing hormone), regulate testicular development and functions in all vertebrates. At the pituitary, different signaling systems regulate the synthesis and secretion of the gonadotropins, such as the hypothalamic neuropeptides GnRH (gonadotropin-releasing hormone) and GnIH (gonadotropin-inhibitory hormone). While GnRH exerts stimulatory roles, the actions of GnIH remain controversial for many teleost species. Therefore, the aim of this study was to evaluate the in vitro effects of chicken GnRH2 (cGnRH2) and zebrafish GnIH-3 (zGnIH-3) on the male gonadotropin and GnRH system expression using pituitary explants and brain slices from a neotropical species with economical and ecological relevance, Astyanax altiparanae. Our results showed that in males, cGnRH2 increased fshb and lhb mRNA levels in the pituitary explants. Interestingly, zGnIH-3 has no effect on basal gonadotropin expression, however zGnIH-3 decreased the cGnRH2-induced fshb and lhb transcripts in male pituitary explants. In the male brain slices, zGnIH-3 showed stimulatory effects, increasing gnrh2 mRNA levels. Overall, our results suggested that GnIH seems to have dual regulatory actions on gonadotropin and GnRH2 expression of A. altiparanae males. This study provided basic information on endocrine regulation of A. altiparanae reproduction, and the obtained results will expand our knowledge, improving the reproductive management of this economically important freshwater species. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:22:12Z 2018-12-11T17:22:12Z 2018-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.ygcen.2018.08.006 General and Comparative Endocrinology. 1095-6840 0016-6480 http://hdl.handle.net/11449/176721 10.1016/j.ygcen.2018.08.006 2-s2.0-85051694269 2-s2.0-85051694269.pdf 0515708585253985 0000-0001-9796-5076 |
url |
http://dx.doi.org/10.1016/j.ygcen.2018.08.006 http://hdl.handle.net/11449/176721 |
identifier_str_mv |
General and Comparative Endocrinology. 1095-6840 0016-6480 10.1016/j.ygcen.2018.08.006 2-s2.0-85051694269 2-s2.0-85051694269.pdf 0515708585253985 0000-0001-9796-5076 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
General and Comparative Endocrinology 1,101 1,101 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128368726507520 |