Paternal effect does not affect in vitro embryo morphokinetics but modulates molecular profile

Detalhes bibliográficos
Autor(a) principal: Guibu de Almeida, Tamie
Data de Publicação: 2022
Outros Autores: Mingoti, Rodolfo Daniel, Signori de Castro, Letícia, Perez Siqueira, Adriano Felipe, Rose dos Santos Hamilton, Thais, Kubo Fontes, Patricia [UNESP], Gouveia Nogueira, Marcelo Fábio [UNESP], Alves, Mayra Fernanda, Basso, Andrea Cristina, Pecora Milazzotto, Marcella, Ortiz D'Avila Assumpção, Mayra Elena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.theriogenology.2021.10.027
http://hdl.handle.net/11449/231549
Resumo: The use of different sires influences in vitro embryo production (IVP) outcome. Paternal effects are observed from the first cleavages until after embryonic genome activation (EGA). Little is known about the mechanisms that promote in vitro fertility differences, even less about the consequences on embryo development. Therefore, this study aimed to evaluate the paternal effect at fertilization, embryo developmental kinetics, gene expression and quality from high and low in vitro fertility bulls. A retrospective analysis for bull selection was performed using the In vitro Brazil company database from 2012 to 2015. The dataset was edited employing cleavage and blastocyst rates ranking a total of 140 bulls. Subsequently, the dataset was restricted by embryo development rate (blastocyst/cleaved rate) and ten bulls were selected as high (HF; n = 5) and low (LF; n = 5) in vitro fertility groups. IVP embryos derived from high and low fertility bulls were classified according to their stage of development (2 cells, 3–4 cells, 6 cells, 8–16 cells), at 24, 36, 48, 60, 72 hpi, respectively, to evaluate embryo kinetics. Pronuclei formation (24 hpi), cleavage rate (Day 3), development rate, and blastocyst morphology (Grade I and II – Day 7) were also assessed, as well as the abundance of 96 transcripts at 8–16 cell stage and blastocysts. There was no difference in early embryo kinetics (P > 0.05), and cleavage rate (HF = 86.7%; LF = 84.9%; P = 0.25). Nevertheless, the fertilization rate was higher on HF (72%) than LF (62%) and the polyspermy rate was lower on HF compared to LF (HF:16.2% LF:29.2%). As expected, blastocyst rate (HF = 29.4%; LF = 16.0%; P < 0.0001) and development rate (HF = 33.9% LF = 18.9%; P < 0.0001) were higher in HF than LF. At the 8–16 cell stage, 22 transcripts were differentially represented (P ≤ 0.05) between the two groups. Only PGK1 and TFAM levels were higher in HF while transcripts related to stress (6/22, ∼27%), cell proliferation (6/22, ∼27%), lipid metabolism genes (5/22, ∼23%), and other cellular functions (5/22, ∼23%) were higher on LF embryos. Blastocysts had 9 differentially represented transcripts (P ≤ 0.05); being only ACSL3 and ELOV1 higher in the HF group. Lipid metabolism genes (3/9, 33%) and other cellular functions (6/9, 67%) were higher in the LF group. In conclusion, the timing of the first cleavages is not affected by in vitro bull fertility. However, low in vitro fertility bulls presented higher polyspermy rates and produced 8–16 cells embryos with higher levels of transcripts related to apoptosis and cell damage pathways compared to high in vitro fertility ones. Evidence such as polyspermy and increase in apoptotic and oxidative stress genes at the EGA stage suggest that embryo development is impaired in the LF group leading to the reduction of blastocyst rate.
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spelling Paternal effect does not affect in vitro embryo morphokinetics but modulates molecular profileEmbryo kineticsGene expressionIn vitro embryo productionPaternal effectThe use of different sires influences in vitro embryo production (IVP) outcome. Paternal effects are observed from the first cleavages until after embryonic genome activation (EGA). Little is known about the mechanisms that promote in vitro fertility differences, even less about the consequences on embryo development. Therefore, this study aimed to evaluate the paternal effect at fertilization, embryo developmental kinetics, gene expression and quality from high and low in vitro fertility bulls. A retrospective analysis for bull selection was performed using the In vitro Brazil company database from 2012 to 2015. The dataset was edited employing cleavage and blastocyst rates ranking a total of 140 bulls. Subsequently, the dataset was restricted by embryo development rate (blastocyst/cleaved rate) and ten bulls were selected as high (HF; n = 5) and low (LF; n = 5) in vitro fertility groups. IVP embryos derived from high and low fertility bulls were classified according to their stage of development (2 cells, 3–4 cells, 6 cells, 8–16 cells), at 24, 36, 48, 60, 72 hpi, respectively, to evaluate embryo kinetics. Pronuclei formation (24 hpi), cleavage rate (Day 3), development rate, and blastocyst morphology (Grade I and II – Day 7) were also assessed, as well as the abundance of 96 transcripts at 8–16 cell stage and blastocysts. There was no difference in early embryo kinetics (P > 0.05), and cleavage rate (HF = 86.7%; LF = 84.9%; P = 0.25). Nevertheless, the fertilization rate was higher on HF (72%) than LF (62%) and the polyspermy rate was lower on HF compared to LF (HF:16.2% LF:29.2%). As expected, blastocyst rate (HF = 29.4%; LF = 16.0%; P < 0.0001) and development rate (HF = 33.9% LF = 18.9%; P < 0.0001) were higher in HF than LF. At the 8–16 cell stage, 22 transcripts were differentially represented (P ≤ 0.05) between the two groups. Only PGK1 and TFAM levels were higher in HF while transcripts related to stress (6/22, ∼27%), cell proliferation (6/22, ∼27%), lipid metabolism genes (5/22, ∼23%), and other cellular functions (5/22, ∼23%) were higher on LF embryos. Blastocysts had 9 differentially represented transcripts (P ≤ 0.05); being only ACSL3 and ELOV1 higher in the HF group. Lipid metabolism genes (3/9, 33%) and other cellular functions (6/9, 67%) were higher in the LF group. In conclusion, the timing of the first cleavages is not affected by in vitro bull fertility. However, low in vitro fertility bulls presented higher polyspermy rates and produced 8–16 cells embryos with higher levels of transcripts related to apoptosis and cell damage pathways compared to high in vitro fertility ones. Evidence such as polyspermy and increase in apoptotic and oxidative stress genes at the EGA stage suggest that embryo development is impaired in the LF group leading to the reduction of blastocyst rate.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratory of Spermatozoa Biology Department of Animal Reproduction School of Veterinary Medicine and Animal Science University of Sao PauloDepartment of Pharmacology Laboratory of Phytomedicines Pharmacology and Biotechnology Institute of Biosciences University of São Paulo State (Unesp), São PauloDepartment of Biological Science School of Sciences and Languages University of São Paulo State, Assis, São PauloIn Vitro Brasil S/A, São PauloCenter of Natural and Human Sciences Federal University of ABCDepartment of Pharmacology Laboratory of Phytomedicines Pharmacology and Biotechnology Institute of Biosciences University of São Paulo State (Unesp), São PauloFAPESP: 2012/50533-2FAPESP: 2016/15147-5Universidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)In Vitro Brasil S/AFederal University of ABCGuibu de Almeida, TamieMingoti, Rodolfo DanielSignori de Castro, LetíciaPerez Siqueira, Adriano FelipeRose dos Santos Hamilton, ThaisKubo Fontes, Patricia [UNESP]Gouveia Nogueira, Marcelo Fábio [UNESP]Alves, Mayra FernandaBasso, Andrea CristinaPecora Milazzotto, MarcellaOrtiz D'Avila Assumpção, Mayra Elena2022-04-29T08:46:05Z2022-04-29T08:46:05Z2022-01-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article30-39http://dx.doi.org/10.1016/j.theriogenology.2021.10.027Theriogenology, v. 178, p. 30-39.0093-691Xhttp://hdl.handle.net/11449/23154910.1016/j.theriogenology.2021.10.0272-s2.0-85118828644Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTheriogenologyinfo:eu-repo/semantics/openAccess2024-09-09T14:05:34Zoai:repositorio.unesp.br:11449/231549Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:05:34Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Paternal effect does not affect in vitro embryo morphokinetics but modulates molecular profile
title Paternal effect does not affect in vitro embryo morphokinetics but modulates molecular profile
spellingShingle Paternal effect does not affect in vitro embryo morphokinetics but modulates molecular profile
Guibu de Almeida, Tamie
Embryo kinetics
Gene expression
In vitro embryo production
Paternal effect
title_short Paternal effect does not affect in vitro embryo morphokinetics but modulates molecular profile
title_full Paternal effect does not affect in vitro embryo morphokinetics but modulates molecular profile
title_fullStr Paternal effect does not affect in vitro embryo morphokinetics but modulates molecular profile
title_full_unstemmed Paternal effect does not affect in vitro embryo morphokinetics but modulates molecular profile
title_sort Paternal effect does not affect in vitro embryo morphokinetics but modulates molecular profile
author Guibu de Almeida, Tamie
author_facet Guibu de Almeida, Tamie
Mingoti, Rodolfo Daniel
Signori de Castro, Letícia
Perez Siqueira, Adriano Felipe
Rose dos Santos Hamilton, Thais
Kubo Fontes, Patricia [UNESP]
Gouveia Nogueira, Marcelo Fábio [UNESP]
Alves, Mayra Fernanda
Basso, Andrea Cristina
Pecora Milazzotto, Marcella
Ortiz D'Avila Assumpção, Mayra Elena
author_role author
author2 Mingoti, Rodolfo Daniel
Signori de Castro, Letícia
Perez Siqueira, Adriano Felipe
Rose dos Santos Hamilton, Thais
Kubo Fontes, Patricia [UNESP]
Gouveia Nogueira, Marcelo Fábio [UNESP]
Alves, Mayra Fernanda
Basso, Andrea Cristina
Pecora Milazzotto, Marcella
Ortiz D'Avila Assumpção, Mayra Elena
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (UNESP)
In Vitro Brasil S/A
Federal University of ABC
dc.contributor.author.fl_str_mv Guibu de Almeida, Tamie
Mingoti, Rodolfo Daniel
Signori de Castro, Letícia
Perez Siqueira, Adriano Felipe
Rose dos Santos Hamilton, Thais
Kubo Fontes, Patricia [UNESP]
Gouveia Nogueira, Marcelo Fábio [UNESP]
Alves, Mayra Fernanda
Basso, Andrea Cristina
Pecora Milazzotto, Marcella
Ortiz D'Avila Assumpção, Mayra Elena
dc.subject.por.fl_str_mv Embryo kinetics
Gene expression
In vitro embryo production
Paternal effect
topic Embryo kinetics
Gene expression
In vitro embryo production
Paternal effect
description The use of different sires influences in vitro embryo production (IVP) outcome. Paternal effects are observed from the first cleavages until after embryonic genome activation (EGA). Little is known about the mechanisms that promote in vitro fertility differences, even less about the consequences on embryo development. Therefore, this study aimed to evaluate the paternal effect at fertilization, embryo developmental kinetics, gene expression and quality from high and low in vitro fertility bulls. A retrospective analysis for bull selection was performed using the In vitro Brazil company database from 2012 to 2015. The dataset was edited employing cleavage and blastocyst rates ranking a total of 140 bulls. Subsequently, the dataset was restricted by embryo development rate (blastocyst/cleaved rate) and ten bulls were selected as high (HF; n = 5) and low (LF; n = 5) in vitro fertility groups. IVP embryos derived from high and low fertility bulls were classified according to their stage of development (2 cells, 3–4 cells, 6 cells, 8–16 cells), at 24, 36, 48, 60, 72 hpi, respectively, to evaluate embryo kinetics. Pronuclei formation (24 hpi), cleavage rate (Day 3), development rate, and blastocyst morphology (Grade I and II – Day 7) were also assessed, as well as the abundance of 96 transcripts at 8–16 cell stage and blastocysts. There was no difference in early embryo kinetics (P > 0.05), and cleavage rate (HF = 86.7%; LF = 84.9%; P = 0.25). Nevertheless, the fertilization rate was higher on HF (72%) than LF (62%) and the polyspermy rate was lower on HF compared to LF (HF:16.2% LF:29.2%). As expected, blastocyst rate (HF = 29.4%; LF = 16.0%; P < 0.0001) and development rate (HF = 33.9% LF = 18.9%; P < 0.0001) were higher in HF than LF. At the 8–16 cell stage, 22 transcripts were differentially represented (P ≤ 0.05) between the two groups. Only PGK1 and TFAM levels were higher in HF while transcripts related to stress (6/22, ∼27%), cell proliferation (6/22, ∼27%), lipid metabolism genes (5/22, ∼23%), and other cellular functions (5/22, ∼23%) were higher on LF embryos. Blastocysts had 9 differentially represented transcripts (P ≤ 0.05); being only ACSL3 and ELOV1 higher in the HF group. Lipid metabolism genes (3/9, 33%) and other cellular functions (6/9, 67%) were higher in the LF group. In conclusion, the timing of the first cleavages is not affected by in vitro bull fertility. However, low in vitro fertility bulls presented higher polyspermy rates and produced 8–16 cells embryos with higher levels of transcripts related to apoptosis and cell damage pathways compared to high in vitro fertility ones. Evidence such as polyspermy and increase in apoptotic and oxidative stress genes at the EGA stage suggest that embryo development is impaired in the LF group leading to the reduction of blastocyst rate.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-29T08:46:05Z
2022-04-29T08:46:05Z
2022-01-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.theriogenology.2021.10.027
Theriogenology, v. 178, p. 30-39.
0093-691X
http://hdl.handle.net/11449/231549
10.1016/j.theriogenology.2021.10.027
2-s2.0-85118828644
url http://dx.doi.org/10.1016/j.theriogenology.2021.10.027
http://hdl.handle.net/11449/231549
identifier_str_mv Theriogenology, v. 178, p. 30-39.
0093-691X
10.1016/j.theriogenology.2021.10.027
2-s2.0-85118828644
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Theriogenology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 30-39
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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