Evaluation of the germicidal action of ultraviolet light in biosafety cabinet concerning bacterial species

Detalhes bibliográficos
Autor(a) principal: Barbosa, Adriana Sierra Assencio Almeida [UNESP]
Data de Publicação: 2021
Outros Autores: de Camargo, Mariana Priscila, de Magalhães Tavares Moreira, Julie Anne Corrêa, Diman, Rafael Balan [UNESP], da Silveira, Mônica [UNESP]
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.11606/issn.2176-7262.rmrp.2021.166597
http://hdl.handle.net/11449/229227
Resumo: Introduction: The biological safety cabinet (BSC) is a collective protective device used to hold aerosols produced in laboratory procedures. The BSC protects workers, material handling, and the environment. It relies on ultraviolet light (UV) lamps that have germicidal action, altering the nucleic acids of microorganisms. Objective: The objective of the present study was to evaluate the germicidal action of BSC class II type A2 UV light against the culture of two bacterial species with different conditions of exposure to UV light. Methods: For the research, the bacteria Staphylococcus aureus ATCC 25923 and Klebsiella pneumoniae ATCC 10031 were used, at a concentration of 1.5x108 Colony Forming Units/ml (CFU/ml), which were seeded in Petri biplates. Use UV light under different conditions and times. Results: The bacterial species showed the same growth or inhibition profile when subjected to different exposure conditions. Petri dishes with the cover open and protected or not with sterilization packaging showed bacterial inhibition at 15 and 20 minutes. The microorganisms in the biplates protected by the aluminum foil and in the biplates with the cover on, regardless of the time and condition, showed bacterial growth. Conclusion: With the results obtained, it is suggested that the germicidal action of UV light was effective, ensuring adequate decontamination and ensuring quality in laboratory biosafety.
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spelling Evaluation of the germicidal action of ultraviolet light in biosafety cabinet concerning bacterial speciesAvaliação da ação germicida da luz ultravioleta de cabine de segurança biológica frente às espécies bacterianasBiosafety cabinetKlebsiella pneumoniaeStaphylococcus aureusUV lightIntroduction: The biological safety cabinet (BSC) is a collective protective device used to hold aerosols produced in laboratory procedures. The BSC protects workers, material handling, and the environment. It relies on ultraviolet light (UV) lamps that have germicidal action, altering the nucleic acids of microorganisms. Objective: The objective of the present study was to evaluate the germicidal action of BSC class II type A2 UV light against the culture of two bacterial species with different conditions of exposure to UV light. Methods: For the research, the bacteria Staphylococcus aureus ATCC 25923 and Klebsiella pneumoniae ATCC 10031 were used, at a concentration of 1.5x108 Colony Forming Units/ml (CFU/ml), which were seeded in Petri biplates. Use UV light under different conditions and times. Results: The bacterial species showed the same growth or inhibition profile when subjected to different exposure conditions. Petri dishes with the cover open and protected or not with sterilization packaging showed bacterial inhibition at 15 and 20 minutes. The microorganisms in the biplates protected by the aluminum foil and in the biplates with the cover on, regardless of the time and condition, showed bacterial growth. Conclusion: With the results obtained, it is suggested that the germicidal action of UV light was effective, ensuring adequate decontamination and ensuring quality in laboratory biosafety.Universidade Estadual Paulista Júlio de Mesquita Filho- UNESPFaculdade de Tecnologia de Bauru- FATEC, SPUniversidade Estadual Paulista Júlio de Mesquita Filho- UNESP Botucatu. Hospital Estadual de Bauru, SPUniversidade Estadual Paulista Júlio de Mesquita Filho- UNESPUniversidade Estadual Paulista Júlio de Mesquita Filho- UNESP Botucatu. Hospital Estadual de Bauru, SPUniversidade Estadual Paulista (UNESP)Faculdade de Tecnologia de Bauru- FATECBarbosa, Adriana Sierra Assencio Almeida [UNESP]de Camargo, Mariana Priscilade Magalhães Tavares Moreira, Julie Anne CorrêaDiman, Rafael Balan [UNESP]da Silveira, Mônica [UNESP]2022-04-29T08:31:20Z2022-04-29T08:31:20Z2021-06-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.11606/issn.2176-7262.rmrp.2021.166597Medicina (Brazil), v. 54, n. 1, 2021.2176-72620076-6046http://hdl.handle.net/11449/22922710.11606/issn.2176-7262.rmrp.2021.1665972-s2.0-85111497227Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporMedicina (Brazil)info:eu-repo/semantics/openAccess2024-09-30T17:35:12Zoai:repositorio.unesp.br:11449/229227Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-30T17:35:12Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Evaluation of the germicidal action of ultraviolet light in biosafety cabinet concerning bacterial species
Avaliação da ação germicida da luz ultravioleta de cabine de segurança biológica frente às espécies bacterianas
title Evaluation of the germicidal action of ultraviolet light in biosafety cabinet concerning bacterial species
spellingShingle Evaluation of the germicidal action of ultraviolet light in biosafety cabinet concerning bacterial species
Barbosa, Adriana Sierra Assencio Almeida [UNESP]
Biosafety cabinet
Klebsiella pneumoniae
Staphylococcus aureus
UV light
title_short Evaluation of the germicidal action of ultraviolet light in biosafety cabinet concerning bacterial species
title_full Evaluation of the germicidal action of ultraviolet light in biosafety cabinet concerning bacterial species
title_fullStr Evaluation of the germicidal action of ultraviolet light in biosafety cabinet concerning bacterial species
title_full_unstemmed Evaluation of the germicidal action of ultraviolet light in biosafety cabinet concerning bacterial species
title_sort Evaluation of the germicidal action of ultraviolet light in biosafety cabinet concerning bacterial species
author Barbosa, Adriana Sierra Assencio Almeida [UNESP]
author_facet Barbosa, Adriana Sierra Assencio Almeida [UNESP]
de Camargo, Mariana Priscila
de Magalhães Tavares Moreira, Julie Anne Corrêa
Diman, Rafael Balan [UNESP]
da Silveira, Mônica [UNESP]
author_role author
author2 de Camargo, Mariana Priscila
de Magalhães Tavares Moreira, Julie Anne Corrêa
Diman, Rafael Balan [UNESP]
da Silveira, Mônica [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Faculdade de Tecnologia de Bauru- FATEC
dc.contributor.author.fl_str_mv Barbosa, Adriana Sierra Assencio Almeida [UNESP]
de Camargo, Mariana Priscila
de Magalhães Tavares Moreira, Julie Anne Corrêa
Diman, Rafael Balan [UNESP]
da Silveira, Mônica [UNESP]
dc.subject.por.fl_str_mv Biosafety cabinet
Klebsiella pneumoniae
Staphylococcus aureus
UV light
topic Biosafety cabinet
Klebsiella pneumoniae
Staphylococcus aureus
UV light
description Introduction: The biological safety cabinet (BSC) is a collective protective device used to hold aerosols produced in laboratory procedures. The BSC protects workers, material handling, and the environment. It relies on ultraviolet light (UV) lamps that have germicidal action, altering the nucleic acids of microorganisms. Objective: The objective of the present study was to evaluate the germicidal action of BSC class II type A2 UV light against the culture of two bacterial species with different conditions of exposure to UV light. Methods: For the research, the bacteria Staphylococcus aureus ATCC 25923 and Klebsiella pneumoniae ATCC 10031 were used, at a concentration of 1.5x108 Colony Forming Units/ml (CFU/ml), which were seeded in Petri biplates. Use UV light under different conditions and times. Results: The bacterial species showed the same growth or inhibition profile when subjected to different exposure conditions. Petri dishes with the cover open and protected or not with sterilization packaging showed bacterial inhibition at 15 and 20 minutes. The microorganisms in the biplates protected by the aluminum foil and in the biplates with the cover on, regardless of the time and condition, showed bacterial growth. Conclusion: With the results obtained, it is suggested that the germicidal action of UV light was effective, ensuring adequate decontamination and ensuring quality in laboratory biosafety.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-02
2022-04-29T08:31:20Z
2022-04-29T08:31:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.11606/issn.2176-7262.rmrp.2021.166597
Medicina (Brazil), v. 54, n. 1, 2021.
2176-7262
0076-6046
http://hdl.handle.net/11449/229227
10.11606/issn.2176-7262.rmrp.2021.166597
2-s2.0-85111497227
url http://dx.doi.org/10.11606/issn.2176-7262.rmrp.2021.166597
http://hdl.handle.net/11449/229227
identifier_str_mv Medicina (Brazil), v. 54, n. 1, 2021.
2176-7262
0076-6046
10.11606/issn.2176-7262.rmrp.2021.166597
2-s2.0-85111497227
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Medicina (Brazil)
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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