Evaluation of the germicidal action of ultraviolet light in biosafety cabinet concerning bacterial species
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.11606/issn.2176-7262.rmrp.2021.166597 http://hdl.handle.net/11449/229227 |
Resumo: | Introduction: The biological safety cabinet (BSC) is a collective protective device used to hold aerosols produced in laboratory procedures. The BSC protects workers, material handling, and the environment. It relies on ultraviolet light (UV) lamps that have germicidal action, altering the nucleic acids of microorganisms. Objective: The objective of the present study was to evaluate the germicidal action of BSC class II type A2 UV light against the culture of two bacterial species with different conditions of exposure to UV light. Methods: For the research, the bacteria Staphylococcus aureus ATCC 25923 and Klebsiella pneumoniae ATCC 10031 were used, at a concentration of 1.5x108 Colony Forming Units/ml (CFU/ml), which were seeded in Petri biplates. Use UV light under different conditions and times. Results: The bacterial species showed the same growth or inhibition profile when subjected to different exposure conditions. Petri dishes with the cover open and protected or not with sterilization packaging showed bacterial inhibition at 15 and 20 minutes. The microorganisms in the biplates protected by the aluminum foil and in the biplates with the cover on, regardless of the time and condition, showed bacterial growth. Conclusion: With the results obtained, it is suggested that the germicidal action of UV light was effective, ensuring adequate decontamination and ensuring quality in laboratory biosafety. |
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Repositório Institucional da UNESP |
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2946 |
spelling |
Evaluation of the germicidal action of ultraviolet light in biosafety cabinet concerning bacterial speciesAvaliação da ação germicida da luz ultravioleta de cabine de segurança biológica frente às espécies bacterianasBiosafety cabinetKlebsiella pneumoniaeStaphylococcus aureusUV lightIntroduction: The biological safety cabinet (BSC) is a collective protective device used to hold aerosols produced in laboratory procedures. The BSC protects workers, material handling, and the environment. It relies on ultraviolet light (UV) lamps that have germicidal action, altering the nucleic acids of microorganisms. Objective: The objective of the present study was to evaluate the germicidal action of BSC class II type A2 UV light against the culture of two bacterial species with different conditions of exposure to UV light. Methods: For the research, the bacteria Staphylococcus aureus ATCC 25923 and Klebsiella pneumoniae ATCC 10031 were used, at a concentration of 1.5x108 Colony Forming Units/ml (CFU/ml), which were seeded in Petri biplates. Use UV light under different conditions and times. Results: The bacterial species showed the same growth or inhibition profile when subjected to different exposure conditions. Petri dishes with the cover open and protected or not with sterilization packaging showed bacterial inhibition at 15 and 20 minutes. The microorganisms in the biplates protected by the aluminum foil and in the biplates with the cover on, regardless of the time and condition, showed bacterial growth. Conclusion: With the results obtained, it is suggested that the germicidal action of UV light was effective, ensuring adequate decontamination and ensuring quality in laboratory biosafety.Universidade Estadual Paulista Júlio de Mesquita Filho- UNESPFaculdade de Tecnologia de Bauru- FATEC, SPUniversidade Estadual Paulista Júlio de Mesquita Filho- UNESP Botucatu. Hospital Estadual de Bauru, SPUniversidade Estadual Paulista Júlio de Mesquita Filho- UNESPUniversidade Estadual Paulista Júlio de Mesquita Filho- UNESP Botucatu. Hospital Estadual de Bauru, SPUniversidade Estadual Paulista (UNESP)Faculdade de Tecnologia de Bauru- FATECBarbosa, Adriana Sierra Assencio Almeida [UNESP]de Camargo, Mariana Priscilade Magalhães Tavares Moreira, Julie Anne CorrêaDiman, Rafael Balan [UNESP]da Silveira, Mônica [UNESP]2022-04-29T08:31:20Z2022-04-29T08:31:20Z2021-06-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.11606/issn.2176-7262.rmrp.2021.166597Medicina (Brazil), v. 54, n. 1, 2021.2176-72620076-6046http://hdl.handle.net/11449/22922710.11606/issn.2176-7262.rmrp.2021.1665972-s2.0-85111497227Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporMedicina (Brazil)info:eu-repo/semantics/openAccess2024-09-30T17:35:12Zoai:repositorio.unesp.br:11449/229227Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-30T17:35:12Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of the germicidal action of ultraviolet light in biosafety cabinet concerning bacterial species Avaliação da ação germicida da luz ultravioleta de cabine de segurança biológica frente às espécies bacterianas |
title |
Evaluation of the germicidal action of ultraviolet light in biosafety cabinet concerning bacterial species |
spellingShingle |
Evaluation of the germicidal action of ultraviolet light in biosafety cabinet concerning bacterial species Barbosa, Adriana Sierra Assencio Almeida [UNESP] Biosafety cabinet Klebsiella pneumoniae Staphylococcus aureus UV light |
title_short |
Evaluation of the germicidal action of ultraviolet light in biosafety cabinet concerning bacterial species |
title_full |
Evaluation of the germicidal action of ultraviolet light in biosafety cabinet concerning bacterial species |
title_fullStr |
Evaluation of the germicidal action of ultraviolet light in biosafety cabinet concerning bacterial species |
title_full_unstemmed |
Evaluation of the germicidal action of ultraviolet light in biosafety cabinet concerning bacterial species |
title_sort |
Evaluation of the germicidal action of ultraviolet light in biosafety cabinet concerning bacterial species |
author |
Barbosa, Adriana Sierra Assencio Almeida [UNESP] |
author_facet |
Barbosa, Adriana Sierra Assencio Almeida [UNESP] de Camargo, Mariana Priscila de Magalhães Tavares Moreira, Julie Anne Corrêa Diman, Rafael Balan [UNESP] da Silveira, Mônica [UNESP] |
author_role |
author |
author2 |
de Camargo, Mariana Priscila de Magalhães Tavares Moreira, Julie Anne Corrêa Diman, Rafael Balan [UNESP] da Silveira, Mônica [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Faculdade de Tecnologia de Bauru- FATEC |
dc.contributor.author.fl_str_mv |
Barbosa, Adriana Sierra Assencio Almeida [UNESP] de Camargo, Mariana Priscila de Magalhães Tavares Moreira, Julie Anne Corrêa Diman, Rafael Balan [UNESP] da Silveira, Mônica [UNESP] |
dc.subject.por.fl_str_mv |
Biosafety cabinet Klebsiella pneumoniae Staphylococcus aureus UV light |
topic |
Biosafety cabinet Klebsiella pneumoniae Staphylococcus aureus UV light |
description |
Introduction: The biological safety cabinet (BSC) is a collective protective device used to hold aerosols produced in laboratory procedures. The BSC protects workers, material handling, and the environment. It relies on ultraviolet light (UV) lamps that have germicidal action, altering the nucleic acids of microorganisms. Objective: The objective of the present study was to evaluate the germicidal action of BSC class II type A2 UV light against the culture of two bacterial species with different conditions of exposure to UV light. Methods: For the research, the bacteria Staphylococcus aureus ATCC 25923 and Klebsiella pneumoniae ATCC 10031 were used, at a concentration of 1.5x108 Colony Forming Units/ml (CFU/ml), which were seeded in Petri biplates. Use UV light under different conditions and times. Results: The bacterial species showed the same growth or inhibition profile when subjected to different exposure conditions. Petri dishes with the cover open and protected or not with sterilization packaging showed bacterial inhibition at 15 and 20 minutes. The microorganisms in the biplates protected by the aluminum foil and in the biplates with the cover on, regardless of the time and condition, showed bacterial growth. Conclusion: With the results obtained, it is suggested that the germicidal action of UV light was effective, ensuring adequate decontamination and ensuring quality in laboratory biosafety. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-02 2022-04-29T08:31:20Z 2022-04-29T08:31:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.11606/issn.2176-7262.rmrp.2021.166597 Medicina (Brazil), v. 54, n. 1, 2021. 2176-7262 0076-6046 http://hdl.handle.net/11449/229227 10.11606/issn.2176-7262.rmrp.2021.166597 2-s2.0-85111497227 |
url |
http://dx.doi.org/10.11606/issn.2176-7262.rmrp.2021.166597 http://hdl.handle.net/11449/229227 |
identifier_str_mv |
Medicina (Brazil), v. 54, n. 1, 2021. 2176-7262 0076-6046 10.11606/issn.2176-7262.rmrp.2021.166597 2-s2.0-85111497227 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Medicina (Brazil) |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546414602452992 |