Fungicide pyraclostrobin affects midgut morphophysiology and reduces survival of Brazilian native stingless bee Melipona scutellaris
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.ecoenv.2020.111395 http://hdl.handle.net/11449/206643 |
Resumo: | Native stingless bees are key pollinators of native flora and important for many crops. However, the loss of natural fragments and exposure to pesticides can hinder the development of colonies and represent a high risk for them. Nevertheless, most studies are conducted with honeybees and there are not many studies on native species, especially in relation to the effects of fungicides on them. Therefore, the objective of this paper is to evaluate the effects of sublethal concentrations of pyraclostrobin, on Melipona scutellaris forager workers. These Brazilian native stingless bees were submitted to continuous oral exposure to three concentrations of pyraclostrobin in sirup: 0.125 ng a.i./µL (P1), 0.025 ng a.i./µL (P2), and 0.005 ng a.i./µL (P3). Histopathological and histochemical parameters of midgut, as well as survival rate were evaluated. All concentrations of fungicide showed an increase in the midgut lesion index and morphological signs of cell death, such as cytoplasmic vacuolizations, presence of atypical nuclei or pyknotic nuclei. Histochemical analyzes revealed a decreased marking of polysaccharides and neutral glycoconjugates both in the villi and in peritrophic membrane in all exposed-groups in relation to control-groups. P1 and P2 groups presented a reduction in total protein marking in digestive cells in relation to control groups. As a consequence of alteration in the midgut, all groups exposed to fungicide showed a reduced survival rate. These findings demonstrate that sublethal concentrations of pyraclostrobin can lead to significant adverse effects in stingless bees. These effects on social native bees indicate the need for reassessment of the safety of fungicides to bees. |
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Fungicide pyraclostrobin affects midgut morphophysiology and reduces survival of Brazilian native stingless bee Melipona scutellarisEcotoxicologyMeliponiniMidgutPyraclostrobinSublethal effectsNative stingless bees are key pollinators of native flora and important for many crops. However, the loss of natural fragments and exposure to pesticides can hinder the development of colonies and represent a high risk for them. Nevertheless, most studies are conducted with honeybees and there are not many studies on native species, especially in relation to the effects of fungicides on them. Therefore, the objective of this paper is to evaluate the effects of sublethal concentrations of pyraclostrobin, on Melipona scutellaris forager workers. These Brazilian native stingless bees were submitted to continuous oral exposure to three concentrations of pyraclostrobin in sirup: 0.125 ng a.i./µL (P1), 0.025 ng a.i./µL (P2), and 0.005 ng a.i./µL (P3). Histopathological and histochemical parameters of midgut, as well as survival rate were evaluated. All concentrations of fungicide showed an increase in the midgut lesion index and morphological signs of cell death, such as cytoplasmic vacuolizations, presence of atypical nuclei or pyknotic nuclei. Histochemical analyzes revealed a decreased marking of polysaccharides and neutral glycoconjugates both in the villi and in peritrophic membrane in all exposed-groups in relation to control-groups. P1 and P2 groups presented a reduction in total protein marking in digestive cells in relation to control groups. As a consequence of alteration in the midgut, all groups exposed to fungicide showed a reduced survival rate. These findings demonstrate that sublethal concentrations of pyraclostrobin can lead to significant adverse effects in stingless bees. These effects on social native bees indicate the need for reassessment of the safety of fungicides to bees.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual Paulista (UNESP) - “Júlio de Mesquita Filho” Instituto de Biociências (IB) Departamento de Biologia Centro de Estudos de Insetos Sociais (CEIS)Universidade Federal de São Carlos (UFSCar) Departamento de Biologia (DBio) NuPECA (Núcleo de Pesquisa em Ecotoxicologia e Conservação de Abelhas) Laboratório de Ecotoxicologia e Análise de Integridade Ambiental (LEIA)Universidade Estadual Paulista (UNESP) - “Júlio de Mesquita Filho” Instituto de Biociências (IB) Departamento de Biologia Centro de Estudos de Insetos Sociais (CEIS)FAPESP: 2016/15743-7FAPESP: 2017/21097-3CNPq: 400540/21097-3Universidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)da Costa Domingues, Caio Eduardo [UNESP]Bello Inoue, Lais Vieira [UNESP]da Silva-Zacarin, Elaine Cristina MathiasMalaspina, Osmar [UNESP]2021-06-25T10:35:42Z2021-06-25T10:35:42Z2020-12-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.ecoenv.2020.111395Ecotoxicology and Environmental Safety, v. 206.1090-24140147-6513http://hdl.handle.net/11449/20664310.1016/j.ecoenv.2020.1113952-s2.0-85092415291Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEcotoxicology and Environmental Safetyinfo:eu-repo/semantics/openAccess2024-04-11T14:57:01Zoai:repositorio.unesp.br:11449/206643Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:41:27.890388Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Fungicide pyraclostrobin affects midgut morphophysiology and reduces survival of Brazilian native stingless bee Melipona scutellaris |
title |
Fungicide pyraclostrobin affects midgut morphophysiology and reduces survival of Brazilian native stingless bee Melipona scutellaris |
spellingShingle |
Fungicide pyraclostrobin affects midgut morphophysiology and reduces survival of Brazilian native stingless bee Melipona scutellaris da Costa Domingues, Caio Eduardo [UNESP] Ecotoxicology Meliponini Midgut Pyraclostrobin Sublethal effects |
title_short |
Fungicide pyraclostrobin affects midgut morphophysiology and reduces survival of Brazilian native stingless bee Melipona scutellaris |
title_full |
Fungicide pyraclostrobin affects midgut morphophysiology and reduces survival of Brazilian native stingless bee Melipona scutellaris |
title_fullStr |
Fungicide pyraclostrobin affects midgut morphophysiology and reduces survival of Brazilian native stingless bee Melipona scutellaris |
title_full_unstemmed |
Fungicide pyraclostrobin affects midgut morphophysiology and reduces survival of Brazilian native stingless bee Melipona scutellaris |
title_sort |
Fungicide pyraclostrobin affects midgut morphophysiology and reduces survival of Brazilian native stingless bee Melipona scutellaris |
author |
da Costa Domingues, Caio Eduardo [UNESP] |
author_facet |
da Costa Domingues, Caio Eduardo [UNESP] Bello Inoue, Lais Vieira [UNESP] da Silva-Zacarin, Elaine Cristina Mathias Malaspina, Osmar [UNESP] |
author_role |
author |
author2 |
Bello Inoue, Lais Vieira [UNESP] da Silva-Zacarin, Elaine Cristina Mathias Malaspina, Osmar [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
da Costa Domingues, Caio Eduardo [UNESP] Bello Inoue, Lais Vieira [UNESP] da Silva-Zacarin, Elaine Cristina Mathias Malaspina, Osmar [UNESP] |
dc.subject.por.fl_str_mv |
Ecotoxicology Meliponini Midgut Pyraclostrobin Sublethal effects |
topic |
Ecotoxicology Meliponini Midgut Pyraclostrobin Sublethal effects |
description |
Native stingless bees are key pollinators of native flora and important for many crops. However, the loss of natural fragments and exposure to pesticides can hinder the development of colonies and represent a high risk for them. Nevertheless, most studies are conducted with honeybees and there are not many studies on native species, especially in relation to the effects of fungicides on them. Therefore, the objective of this paper is to evaluate the effects of sublethal concentrations of pyraclostrobin, on Melipona scutellaris forager workers. These Brazilian native stingless bees were submitted to continuous oral exposure to three concentrations of pyraclostrobin in sirup: 0.125 ng a.i./µL (P1), 0.025 ng a.i./µL (P2), and 0.005 ng a.i./µL (P3). Histopathological and histochemical parameters of midgut, as well as survival rate were evaluated. All concentrations of fungicide showed an increase in the midgut lesion index and morphological signs of cell death, such as cytoplasmic vacuolizations, presence of atypical nuclei or pyknotic nuclei. Histochemical analyzes revealed a decreased marking of polysaccharides and neutral glycoconjugates both in the villi and in peritrophic membrane in all exposed-groups in relation to control-groups. P1 and P2 groups presented a reduction in total protein marking in digestive cells in relation to control groups. As a consequence of alteration in the midgut, all groups exposed to fungicide showed a reduced survival rate. These findings demonstrate that sublethal concentrations of pyraclostrobin can lead to significant adverse effects in stingless bees. These effects on social native bees indicate the need for reassessment of the safety of fungicides to bees. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-15 2021-06-25T10:35:42Z 2021-06-25T10:35:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.ecoenv.2020.111395 Ecotoxicology and Environmental Safety, v. 206. 1090-2414 0147-6513 http://hdl.handle.net/11449/206643 10.1016/j.ecoenv.2020.111395 2-s2.0-85092415291 |
url |
http://dx.doi.org/10.1016/j.ecoenv.2020.111395 http://hdl.handle.net/11449/206643 |
identifier_str_mv |
Ecotoxicology and Environmental Safety, v. 206. 1090-2414 0147-6513 10.1016/j.ecoenv.2020.111395 2-s2.0-85092415291 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ecotoxicology and Environmental Safety |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128265875881984 |