A simple spectrophotometry method for the determination of tetracycline and doxycycline in pharmaceutical formulations using chloramine-T

Detalhes bibliográficos
Autor(a) principal: Rufino, José L. [UNESP]
Data de Publicação: 2010
Outros Autores: Pezza, Helena Redigolo [UNESP], Pezza, Leonardo [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0100-46702010000400018
http://hdl.handle.net/11449/72075
Resumo: A simple, rapid, accurate and inexpensive spectrophotometric method for the determination of tetracycline and doxycycline has been developed. The method is based on the reaction between these drugs and chloramine-T in alkaline medium producing red color products with absorbance maximum at the λ=535 and 525 nm for the tetracycline and doxycycline, respectively. The best conditions for the reactions have been found using multivariate method. Beer's law is obeyed in a concentration ranges 1. 03 x 10 -5 to 3. 61 x 10 4mol L -1 and 1. 75 x 10 -5 to 3. 48 x 10 4mol L -1 for the tetracycline and doxycycline, respectively. The quantification limits were 5. 63 x 10 -6 mol L -1 and 7. 12 x 10 -7 mol L -1 for the tetracycline and doxycycline, respectively. The proposed method was successfully applied to the determination of these drugs in pharmaceutical formulations and the results obtained were in good agreement with those obtained by the comparative method at the 95% confidence level.
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spelling A simple spectrophotometry method for the determination of tetracycline and doxycycline in pharmaceutical formulations using chloramine-TChloramine-TDoxycyclineSpectrophotometricTetracyclineA simple, rapid, accurate and inexpensive spectrophotometric method for the determination of tetracycline and doxycycline has been developed. The method is based on the reaction between these drugs and chloramine-T in alkaline medium producing red color products with absorbance maximum at the λ=535 and 525 nm for the tetracycline and doxycycline, respectively. The best conditions for the reactions have been found using multivariate method. Beer's law is obeyed in a concentration ranges 1. 03 x 10 -5 to 3. 61 x 10 4mol L -1 and 1. 75 x 10 -5 to 3. 48 x 10 4mol L -1 for the tetracycline and doxycycline, respectively. The quantification limits were 5. 63 x 10 -6 mol L -1 and 7. 12 x 10 -7 mol L -1 for the tetracycline and doxycycline, respectively. The proposed method was successfully applied to the determination of these drugs in pharmaceutical formulations and the results obtained were in good agreement with those obtained by the comparative method at the 95% confidence level.São Paulo State University - UNESP Institute of Chemistry of Araraquara, P. O. Box 355, 14801-970, Araraquara, SPSão Paulo State University - UNESP Institute of Chemistry of Araraquara, P. O. Box 355, 14801-970, Araraquara, SPUniversidade Estadual Paulista (Unesp)Rufino, José L. [UNESP]Pezza, Helena Redigolo [UNESP]Pezza, Leonardo [UNESP]2014-05-27T11:25:21Z2014-05-27T11:25:21Z2010-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article139-146application/pdfhttp://dx.doi.org/10.1590/S0100-46702010000400018Ecletica Quimica, v. 35, n. 4, p. 139-146, 2010.0100-4670http://hdl.handle.net/11449/7207510.1590/S0100-46702010000400018S0100-467020100004000182-s2.0-800526065362-s2.0-80052606536.pdf319212509836112159789085918535240000-0003-0197-7369Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEclética Químicainfo:eu-repo/semantics/openAccess2023-12-01T06:14:50Zoai:repositorio.unesp.br:11449/72075Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:13:36.082680Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv A simple spectrophotometry method for the determination of tetracycline and doxycycline in pharmaceutical formulations using chloramine-T
title A simple spectrophotometry method for the determination of tetracycline and doxycycline in pharmaceutical formulations using chloramine-T
spellingShingle A simple spectrophotometry method for the determination of tetracycline and doxycycline in pharmaceutical formulations using chloramine-T
Rufino, José L. [UNESP]
Chloramine-T
Doxycycline
Spectrophotometric
Tetracycline
title_short A simple spectrophotometry method for the determination of tetracycline and doxycycline in pharmaceutical formulations using chloramine-T
title_full A simple spectrophotometry method for the determination of tetracycline and doxycycline in pharmaceutical formulations using chloramine-T
title_fullStr A simple spectrophotometry method for the determination of tetracycline and doxycycline in pharmaceutical formulations using chloramine-T
title_full_unstemmed A simple spectrophotometry method for the determination of tetracycline and doxycycline in pharmaceutical formulations using chloramine-T
title_sort A simple spectrophotometry method for the determination of tetracycline and doxycycline in pharmaceutical formulations using chloramine-T
author Rufino, José L. [UNESP]
author_facet Rufino, José L. [UNESP]
Pezza, Helena Redigolo [UNESP]
Pezza, Leonardo [UNESP]
author_role author
author2 Pezza, Helena Redigolo [UNESP]
Pezza, Leonardo [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Rufino, José L. [UNESP]
Pezza, Helena Redigolo [UNESP]
Pezza, Leonardo [UNESP]
dc.subject.por.fl_str_mv Chloramine-T
Doxycycline
Spectrophotometric
Tetracycline
topic Chloramine-T
Doxycycline
Spectrophotometric
Tetracycline
description A simple, rapid, accurate and inexpensive spectrophotometric method for the determination of tetracycline and doxycycline has been developed. The method is based on the reaction between these drugs and chloramine-T in alkaline medium producing red color products with absorbance maximum at the λ=535 and 525 nm for the tetracycline and doxycycline, respectively. The best conditions for the reactions have been found using multivariate method. Beer's law is obeyed in a concentration ranges 1. 03 x 10 -5 to 3. 61 x 10 4mol L -1 and 1. 75 x 10 -5 to 3. 48 x 10 4mol L -1 for the tetracycline and doxycycline, respectively. The quantification limits were 5. 63 x 10 -6 mol L -1 and 7. 12 x 10 -7 mol L -1 for the tetracycline and doxycycline, respectively. The proposed method was successfully applied to the determination of these drugs in pharmaceutical formulations and the results obtained were in good agreement with those obtained by the comparative method at the 95% confidence level.
publishDate 2010
dc.date.none.fl_str_mv 2010-12-01
2014-05-27T11:25:21Z
2014-05-27T11:25:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-46702010000400018
Ecletica Quimica, v. 35, n. 4, p. 139-146, 2010.
0100-4670
http://hdl.handle.net/11449/72075
10.1590/S0100-46702010000400018
S0100-46702010000400018
2-s2.0-80052606536
2-s2.0-80052606536.pdf
3192125098361121
5978908591853524
0000-0003-0197-7369
url http://dx.doi.org/10.1590/S0100-46702010000400018
http://hdl.handle.net/11449/72075
identifier_str_mv Ecletica Quimica, v. 35, n. 4, p. 139-146, 2010.
0100-4670
10.1590/S0100-46702010000400018
S0100-46702010000400018
2-s2.0-80052606536
2-s2.0-80052606536.pdf
3192125098361121
5978908591853524
0000-0003-0197-7369
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Eclética Química
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 139-146
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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