Subpathotypes of avian pathogenic escherichia coli (apec) exist as defined by their syndromes and virulence traits
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.2174/1874285801105010055 http://hdl.handle.net/11449/219691 |
Resumo: | Avian pathogenic Escherichia coli (APEC) strains cause different types of systemic extraintestinal infections in poultry, collectively termed colibacillosis, which can cause significant economic losses in the poultry industry. To date, there have been no descriptions of genes or characteristics that allow for the classification of avian strains pathotypes responsible for causing specific diseases in their hosts. In this study we aimed to characterize avian E. coli strains representing 4 groups, including one of commensal strains (AFEC - Avian Fecal Escherichia coli) and 3 groups of APEC strains, where each group is responsible for causing a different disease syndrome in their respective hosts (septicemia, omphalitis and swollen head syndrome). We chose to examine several biological characteristics of these strains including: adhesion to eukaryotic cells, pathogenicity levels according to the lethal dose (50%) assay, phylogenetic group and virulence gene profiles. The comparison of strains based on these genotypic and phenotypic traits, using multivariate statisticals tools and complex networks, allowed us to infer information about the population structure of the studied groups. Our results indicate that APEC strains do not constitute a unique homogeneous group, but rather a structured set of subgroups, where each one is associated with a specific infectious syndrome which can possibly be used to define pathotypes or subpathotypes within APEC strains. These results offer new possibilities with which to study the genes responsible for various pathogenetic processes within APEC strains, and for vaccine development. It may be important to consider these subgroups when developing a vaccine in an effort for obtain cross protection, which has not yet been successfully accomplished when working with APEC strains. |
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Subpathotypes of avian pathogenic escherichia coli (apec) exist as defined by their syndromes and virulence traitsApecEscherichia coliNetwork analysisPathotypeAvian pathogenic Escherichia coli (APEC) strains cause different types of systemic extraintestinal infections in poultry, collectively termed colibacillosis, which can cause significant economic losses in the poultry industry. To date, there have been no descriptions of genes or characteristics that allow for the classification of avian strains pathotypes responsible for causing specific diseases in their hosts. In this study we aimed to characterize avian E. coli strains representing 4 groups, including one of commensal strains (AFEC - Avian Fecal Escherichia coli) and 3 groups of APEC strains, where each group is responsible for causing a different disease syndrome in their respective hosts (septicemia, omphalitis and swollen head syndrome). We chose to examine several biological characteristics of these strains including: adhesion to eukaryotic cells, pathogenicity levels according to the lethal dose (50%) assay, phylogenetic group and virulence gene profiles. The comparison of strains based on these genotypic and phenotypic traits, using multivariate statisticals tools and complex networks, allowed us to infer information about the population structure of the studied groups. Our results indicate that APEC strains do not constitute a unique homogeneous group, but rather a structured set of subgroups, where each one is associated with a specific infectious syndrome which can possibly be used to define pathotypes or subpathotypes within APEC strains. These results offer new possibilities with which to study the genes responsible for various pathogenetic processes within APEC strains, and for vaccine development. It may be important to consider these subgroups when developing a vaccine in an effort for obtain cross protection, which has not yet been successfully accomplished when working with APEC strains.Department of Genetics Evolution and Bioagentes, Biology Institute Campinas State University-UNICAMP, Campinas, SPMolecular Biology and Genetic Engineering Center Campinas State University-UNICAMP, Campinas, SPCentro de Biologia Molecular e Engenharia Genética UNICAMP, CampinasDepartment of Ecology Biology Institute São Paulo State University-USP, São Paulo, SPEcology Post-Grad Program, USP, São Paulo, SPGenetics and Biochemistry Institute UFU, Uberlândia, MGDepartment of Microbiology UEL, Londrina, PRDepartment of Toxicological and Bromatologic Clinical Analyses USP, Ribeirão Preto, SPDepartmente of Veterinary and Microbiological Sciences North Dakota State University, Fargo, ND 58108College of Veterinary Medicine Iowa State University, Ames, IA 50011Department of Ecology Biology Institute São Paulo State University-USP, São Paulo, SPUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)UFUUELNorth Dakota State UniversityIowa State UniversityMaturana, Victor Gonçalvesde Pace, FernandaCarlos, CamilaPires, Mathias Mistretta [UNESP]De Campos, Tatiana AmabileNakazato, GersonStheling, Eliana GuedesLogue, Catherine M.Nolan, Lisa K.Da Silveira, Wanderley Dias2022-04-28T18:56:55Z2022-04-28T18:56:55Z2011-08-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article55-64http://dx.doi.org/10.2174/1874285801105010055Open Microbiology Journal, v. 5, n. SUPPL.1, p. 55-64, 2011.1874-2858http://hdl.handle.net/11449/21969110.2174/18742858011050100552-s2.0-79961094449Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOpen Microbiology Journalinfo:eu-repo/semantics/openAccess2022-04-28T18:56:55Zoai:repositorio.unesp.br:11449/219691Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:52:29.959310Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Subpathotypes of avian pathogenic escherichia coli (apec) exist as defined by their syndromes and virulence traits |
title |
Subpathotypes of avian pathogenic escherichia coli (apec) exist as defined by their syndromes and virulence traits |
spellingShingle |
Subpathotypes of avian pathogenic escherichia coli (apec) exist as defined by their syndromes and virulence traits Maturana, Victor Gonçalves Apec Escherichia coli Network analysis Pathotype |
title_short |
Subpathotypes of avian pathogenic escherichia coli (apec) exist as defined by their syndromes and virulence traits |
title_full |
Subpathotypes of avian pathogenic escherichia coli (apec) exist as defined by their syndromes and virulence traits |
title_fullStr |
Subpathotypes of avian pathogenic escherichia coli (apec) exist as defined by their syndromes and virulence traits |
title_full_unstemmed |
Subpathotypes of avian pathogenic escherichia coli (apec) exist as defined by their syndromes and virulence traits |
title_sort |
Subpathotypes of avian pathogenic escherichia coli (apec) exist as defined by their syndromes and virulence traits |
author |
Maturana, Victor Gonçalves |
author_facet |
Maturana, Victor Gonçalves de Pace, Fernanda Carlos, Camila Pires, Mathias Mistretta [UNESP] De Campos, Tatiana Amabile Nakazato, Gerson Stheling, Eliana Guedes Logue, Catherine M. Nolan, Lisa K. Da Silveira, Wanderley Dias |
author_role |
author |
author2 |
de Pace, Fernanda Carlos, Camila Pires, Mathias Mistretta [UNESP] De Campos, Tatiana Amabile Nakazato, Gerson Stheling, Eliana Guedes Logue, Catherine M. Nolan, Lisa K. Da Silveira, Wanderley Dias |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) UFU UEL North Dakota State University Iowa State University |
dc.contributor.author.fl_str_mv |
Maturana, Victor Gonçalves de Pace, Fernanda Carlos, Camila Pires, Mathias Mistretta [UNESP] De Campos, Tatiana Amabile Nakazato, Gerson Stheling, Eliana Guedes Logue, Catherine M. Nolan, Lisa K. Da Silveira, Wanderley Dias |
dc.subject.por.fl_str_mv |
Apec Escherichia coli Network analysis Pathotype |
topic |
Apec Escherichia coli Network analysis Pathotype |
description |
Avian pathogenic Escherichia coli (APEC) strains cause different types of systemic extraintestinal infections in poultry, collectively termed colibacillosis, which can cause significant economic losses in the poultry industry. To date, there have been no descriptions of genes or characteristics that allow for the classification of avian strains pathotypes responsible for causing specific diseases in their hosts. In this study we aimed to characterize avian E. coli strains representing 4 groups, including one of commensal strains (AFEC - Avian Fecal Escherichia coli) and 3 groups of APEC strains, where each group is responsible for causing a different disease syndrome in their respective hosts (septicemia, omphalitis and swollen head syndrome). We chose to examine several biological characteristics of these strains including: adhesion to eukaryotic cells, pathogenicity levels according to the lethal dose (50%) assay, phylogenetic group and virulence gene profiles. The comparison of strains based on these genotypic and phenotypic traits, using multivariate statisticals tools and complex networks, allowed us to infer information about the population structure of the studied groups. Our results indicate that APEC strains do not constitute a unique homogeneous group, but rather a structured set of subgroups, where each one is associated with a specific infectious syndrome which can possibly be used to define pathotypes or subpathotypes within APEC strains. These results offer new possibilities with which to study the genes responsible for various pathogenetic processes within APEC strains, and for vaccine development. It may be important to consider these subgroups when developing a vaccine in an effort for obtain cross protection, which has not yet been successfully accomplished when working with APEC strains. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-08-08 2022-04-28T18:56:55Z 2022-04-28T18:56:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.2174/1874285801105010055 Open Microbiology Journal, v. 5, n. SUPPL.1, p. 55-64, 2011. 1874-2858 http://hdl.handle.net/11449/219691 10.2174/1874285801105010055 2-s2.0-79961094449 |
url |
http://dx.doi.org/10.2174/1874285801105010055 http://hdl.handle.net/11449/219691 |
identifier_str_mv |
Open Microbiology Journal, v. 5, n. SUPPL.1, p. 55-64, 2011. 1874-2858 10.2174/1874285801105010055 2-s2.0-79961094449 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Open Microbiology Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
55-64 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129560781258752 |