Subpathotypes of avian pathogenic escherichia coli (apec) exist as defined by their syndromes and virulence traits

Detalhes bibliográficos
Autor(a) principal: Maturana, Victor Gonçalves
Data de Publicação: 2011
Outros Autores: de Pace, Fernanda, Carlos, Camila, Pires, Mathias Mistretta [UNESP], De Campos, Tatiana Amabile, Nakazato, Gerson, Stheling, Eliana Guedes, Logue, Catherine M., Nolan, Lisa K., Da Silveira, Wanderley Dias
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.2174/1874285801105010055
http://hdl.handle.net/11449/219691
Resumo: Avian pathogenic Escherichia coli (APEC) strains cause different types of systemic extraintestinal infections in poultry, collectively termed colibacillosis, which can cause significant economic losses in the poultry industry. To date, there have been no descriptions of genes or characteristics that allow for the classification of avian strains pathotypes responsible for causing specific diseases in their hosts. In this study we aimed to characterize avian E. coli strains representing 4 groups, including one of commensal strains (AFEC - Avian Fecal Escherichia coli) and 3 groups of APEC strains, where each group is responsible for causing a different disease syndrome in their respective hosts (septicemia, omphalitis and swollen head syndrome). We chose to examine several biological characteristics of these strains including: adhesion to eukaryotic cells, pathogenicity levels according to the lethal dose (50%) assay, phylogenetic group and virulence gene profiles. The comparison of strains based on these genotypic and phenotypic traits, using multivariate statisticals tools and complex networks, allowed us to infer information about the population structure of the studied groups. Our results indicate that APEC strains do not constitute a unique homogeneous group, but rather a structured set of subgroups, where each one is associated with a specific infectious syndrome which can possibly be used to define pathotypes or subpathotypes within APEC strains. These results offer new possibilities with which to study the genes responsible for various pathogenetic processes within APEC strains, and for vaccine development. It may be important to consider these subgroups when developing a vaccine in an effort for obtain cross protection, which has not yet been successfully accomplished when working with APEC strains.
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spelling Subpathotypes of avian pathogenic escherichia coli (apec) exist as defined by their syndromes and virulence traitsApecEscherichia coliNetwork analysisPathotypeAvian pathogenic Escherichia coli (APEC) strains cause different types of systemic extraintestinal infections in poultry, collectively termed colibacillosis, which can cause significant economic losses in the poultry industry. To date, there have been no descriptions of genes or characteristics that allow for the classification of avian strains pathotypes responsible for causing specific diseases in their hosts. In this study we aimed to characterize avian E. coli strains representing 4 groups, including one of commensal strains (AFEC - Avian Fecal Escherichia coli) and 3 groups of APEC strains, where each group is responsible for causing a different disease syndrome in their respective hosts (septicemia, omphalitis and swollen head syndrome). We chose to examine several biological characteristics of these strains including: adhesion to eukaryotic cells, pathogenicity levels according to the lethal dose (50%) assay, phylogenetic group and virulence gene profiles. The comparison of strains based on these genotypic and phenotypic traits, using multivariate statisticals tools and complex networks, allowed us to infer information about the population structure of the studied groups. Our results indicate that APEC strains do not constitute a unique homogeneous group, but rather a structured set of subgroups, where each one is associated with a specific infectious syndrome which can possibly be used to define pathotypes or subpathotypes within APEC strains. These results offer new possibilities with which to study the genes responsible for various pathogenetic processes within APEC strains, and for vaccine development. It may be important to consider these subgroups when developing a vaccine in an effort for obtain cross protection, which has not yet been successfully accomplished when working with APEC strains.Department of Genetics Evolution and Bioagentes, Biology Institute Campinas State University-UNICAMP, Campinas, SPMolecular Biology and Genetic Engineering Center Campinas State University-UNICAMP, Campinas, SPCentro de Biologia Molecular e Engenharia Genética UNICAMP, CampinasDepartment of Ecology Biology Institute São Paulo State University-USP, São Paulo, SPEcology Post-Grad Program, USP, São Paulo, SPGenetics and Biochemistry Institute UFU, Uberlândia, MGDepartment of Microbiology UEL, Londrina, PRDepartment of Toxicological and Bromatologic Clinical Analyses USP, Ribeirão Preto, SPDepartmente of Veterinary and Microbiological Sciences North Dakota State University, Fargo, ND 58108College of Veterinary Medicine Iowa State University, Ames, IA 50011Department of Ecology Biology Institute São Paulo State University-USP, São Paulo, SPUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)UFUUELNorth Dakota State UniversityIowa State UniversityMaturana, Victor Gonçalvesde Pace, FernandaCarlos, CamilaPires, Mathias Mistretta [UNESP]De Campos, Tatiana AmabileNakazato, GersonStheling, Eliana GuedesLogue, Catherine M.Nolan, Lisa K.Da Silveira, Wanderley Dias2022-04-28T18:56:55Z2022-04-28T18:56:55Z2011-08-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article55-64http://dx.doi.org/10.2174/1874285801105010055Open Microbiology Journal, v. 5, n. SUPPL.1, p. 55-64, 2011.1874-2858http://hdl.handle.net/11449/21969110.2174/18742858011050100552-s2.0-79961094449Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOpen Microbiology Journalinfo:eu-repo/semantics/openAccess2022-04-28T18:56:55Zoai:repositorio.unesp.br:11449/219691Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:52:29.959310Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Subpathotypes of avian pathogenic escherichia coli (apec) exist as defined by their syndromes and virulence traits
title Subpathotypes of avian pathogenic escherichia coli (apec) exist as defined by their syndromes and virulence traits
spellingShingle Subpathotypes of avian pathogenic escherichia coli (apec) exist as defined by their syndromes and virulence traits
Maturana, Victor Gonçalves
Apec
Escherichia coli
Network analysis
Pathotype
title_short Subpathotypes of avian pathogenic escherichia coli (apec) exist as defined by their syndromes and virulence traits
title_full Subpathotypes of avian pathogenic escherichia coli (apec) exist as defined by their syndromes and virulence traits
title_fullStr Subpathotypes of avian pathogenic escherichia coli (apec) exist as defined by their syndromes and virulence traits
title_full_unstemmed Subpathotypes of avian pathogenic escherichia coli (apec) exist as defined by their syndromes and virulence traits
title_sort Subpathotypes of avian pathogenic escherichia coli (apec) exist as defined by their syndromes and virulence traits
author Maturana, Victor Gonçalves
author_facet Maturana, Victor Gonçalves
de Pace, Fernanda
Carlos, Camila
Pires, Mathias Mistretta [UNESP]
De Campos, Tatiana Amabile
Nakazato, Gerson
Stheling, Eliana Guedes
Logue, Catherine M.
Nolan, Lisa K.
Da Silveira, Wanderley Dias
author_role author
author2 de Pace, Fernanda
Carlos, Camila
Pires, Mathias Mistretta [UNESP]
De Campos, Tatiana Amabile
Nakazato, Gerson
Stheling, Eliana Guedes
Logue, Catherine M.
Nolan, Lisa K.
Da Silveira, Wanderley Dias
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Campinas (UNICAMP)
Universidade Estadual Paulista (UNESP)
Universidade de São Paulo (USP)
UFU
UEL
North Dakota State University
Iowa State University
dc.contributor.author.fl_str_mv Maturana, Victor Gonçalves
de Pace, Fernanda
Carlos, Camila
Pires, Mathias Mistretta [UNESP]
De Campos, Tatiana Amabile
Nakazato, Gerson
Stheling, Eliana Guedes
Logue, Catherine M.
Nolan, Lisa K.
Da Silveira, Wanderley Dias
dc.subject.por.fl_str_mv Apec
Escherichia coli
Network analysis
Pathotype
topic Apec
Escherichia coli
Network analysis
Pathotype
description Avian pathogenic Escherichia coli (APEC) strains cause different types of systemic extraintestinal infections in poultry, collectively termed colibacillosis, which can cause significant economic losses in the poultry industry. To date, there have been no descriptions of genes or characteristics that allow for the classification of avian strains pathotypes responsible for causing specific diseases in their hosts. In this study we aimed to characterize avian E. coli strains representing 4 groups, including one of commensal strains (AFEC - Avian Fecal Escherichia coli) and 3 groups of APEC strains, where each group is responsible for causing a different disease syndrome in their respective hosts (septicemia, omphalitis and swollen head syndrome). We chose to examine several biological characteristics of these strains including: adhesion to eukaryotic cells, pathogenicity levels according to the lethal dose (50%) assay, phylogenetic group and virulence gene profiles. The comparison of strains based on these genotypic and phenotypic traits, using multivariate statisticals tools and complex networks, allowed us to infer information about the population structure of the studied groups. Our results indicate that APEC strains do not constitute a unique homogeneous group, but rather a structured set of subgroups, where each one is associated with a specific infectious syndrome which can possibly be used to define pathotypes or subpathotypes within APEC strains. These results offer new possibilities with which to study the genes responsible for various pathogenetic processes within APEC strains, and for vaccine development. It may be important to consider these subgroups when developing a vaccine in an effort for obtain cross protection, which has not yet been successfully accomplished when working with APEC strains.
publishDate 2011
dc.date.none.fl_str_mv 2011-08-08
2022-04-28T18:56:55Z
2022-04-28T18:56:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.2174/1874285801105010055
Open Microbiology Journal, v. 5, n. SUPPL.1, p. 55-64, 2011.
1874-2858
http://hdl.handle.net/11449/219691
10.2174/1874285801105010055
2-s2.0-79961094449
url http://dx.doi.org/10.2174/1874285801105010055
http://hdl.handle.net/11449/219691
identifier_str_mv Open Microbiology Journal, v. 5, n. SUPPL.1, p. 55-64, 2011.
1874-2858
10.2174/1874285801105010055
2-s2.0-79961094449
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Open Microbiology Journal
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 55-64
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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