In vivo storage of oocytes leads to lower survival, increased abnormalities and may affect the ploidy status in the yellowtail tetra Astyanax altiparanae
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1017/S0967199418000527 http://hdl.handle.net/11449/185505 |
Resumo: | In this study we analyzed whether the in vivo storage of oocytes (time after ovulation until fertilization) affects the survival and the ploidy status of the yellowtail tetra Astyanax altiparanae. Fish were induced to spawn and, after ovulation, a small aliquot was stripped and immediately fertilized (positive control group). Subsequently, aliquots (similar to 150 oocytes) were stripped and fertilized at various time points of 60, 120, 180 or 240 min. Developmental stages, abnormalities, survival and the ploidy status of the hatched larvae were examined. As expected, in the control group, 100% of the larvae were diploid. Conversely, triploid individuals were observed just at the 60 min treatment time point (0.6%). In vivo storage of oocytes also influenced the survival rates (P < 0.05); the 180 and 240 min samples, respectively, presented lower survival rates at gastrula (50.10 +/- 6.26% and 40.92 +/- 5.32%), and somite (17.80 +/- 5.14% and 4.41 +/- 2.76%) stages and lower hatching rates (12.01 +/- 4.04% and 4.41 +/- 2.76%). A higher percentage (99.27 +/- 0.40%) of normal larvae and only a few abnormal larvae (0.73 +/- 0.40%) were observed in the control group (P = 0.0000). This observation did not differ from that observed at the 60 min treatment point (P = 0.9976). A significant increase in the percentage of abnormalities was observed in the other treatments, and, after 240 min, the highest percentage of abnormal larvae was seen (P=0.0024; 83.33 +/- 16.67%). In conclusion, we showed that oocyte ageing had a significant effect on survival and may affect the ploidy status in A. atiparanae. |
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In vivo storage of oocytes leads to lower survival, increased abnormalities and may affect the ploidy status in the yellowtail tetra Astyanax altiparanaeAgeing of oocytesNatural triploidOver-ripeningPolyploidy, ReproductionIn this study we analyzed whether the in vivo storage of oocytes (time after ovulation until fertilization) affects the survival and the ploidy status of the yellowtail tetra Astyanax altiparanae. Fish were induced to spawn and, after ovulation, a small aliquot was stripped and immediately fertilized (positive control group). Subsequently, aliquots (similar to 150 oocytes) were stripped and fertilized at various time points of 60, 120, 180 or 240 min. Developmental stages, abnormalities, survival and the ploidy status of the hatched larvae were examined. As expected, in the control group, 100% of the larvae were diploid. Conversely, triploid individuals were observed just at the 60 min treatment time point (0.6%). In vivo storage of oocytes also influenced the survival rates (P < 0.05); the 180 and 240 min samples, respectively, presented lower survival rates at gastrula (50.10 +/- 6.26% and 40.92 +/- 5.32%), and somite (17.80 +/- 5.14% and 4.41 +/- 2.76%) stages and lower hatching rates (12.01 +/- 4.04% and 4.41 +/- 2.76%). A higher percentage (99.27 +/- 0.40%) of normal larvae and only a few abnormal larvae (0.73 +/- 0.40%) were observed in the control group (P = 0.0000). This observation did not differ from that observed at the 60 min treatment point (P = 0.9976). A significant increase in the percentage of abnormalities was observed in the other treatments, and, after 240 min, the highest percentage of abnormal larvae was seen (P=0.0024; 83.33 +/- 16.67%). In conclusion, we showed that oocyte ageing had a significant effect on survival and may affect the ploidy status in A. atiparanae.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ, Aquaculture Ctr, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilChico Mendes Inst Biodivers Conservat, Natl Ctr Res & Conservat Continental Fish, Lab Fish Biotechnol, Rodovia Pref Euberto Nemesio Pereira de Godoy, BR-13630970 Pirassununga, SP, BrazilAES Tiete, Br 153,0 Km 139 Ctr, BR-16370000 Promissio, SP, BrazilSao Paulo State Univ, Aquaculture Ctr, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilFAPESP: FAPESP 2016/12383-0FAPESP: FAPESP 2010/17429-1 e 2011/11664-1Cambridge Univ PressUniversidade Estadual Paulista (Unesp)Chico Mendes Inst Biodivers ConservatAES TieteNascimento, Nivaldo Ferreira do [UNESP]Lazaro, Talita Maria [UNESP]Alcantara, Nathalia Raissa de [UNESP]RochaSenhorini, Jose AugustoSantos, Silvio Carlos Alves dosNakaghi, Laura Satiko Okada [UNESP]Yasui, George Shigueki2019-10-04T12:36:02Z2019-10-04T12:36:02Z2018-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article471-475http://dx.doi.org/10.1017/S0967199418000527Zygote. New York: Cambridge Univ Press, v. 26, n. 6, p. 471-475, 2018.0967-1994http://hdl.handle.net/11449/18550510.1017/S0967199418000527WOS:000460557500006Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengZygoteinfo:eu-repo/semantics/openAccess2024-04-09T15:37:02Zoai:repositorio.unesp.br:11449/185505Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-09T15:37:02Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In vivo storage of oocytes leads to lower survival, increased abnormalities and may affect the ploidy status in the yellowtail tetra Astyanax altiparanae |
title |
In vivo storage of oocytes leads to lower survival, increased abnormalities and may affect the ploidy status in the yellowtail tetra Astyanax altiparanae |
spellingShingle |
In vivo storage of oocytes leads to lower survival, increased abnormalities and may affect the ploidy status in the yellowtail tetra Astyanax altiparanae Nascimento, Nivaldo Ferreira do [UNESP] Ageing of oocytes Natural triploid Over-ripening Polyploidy, Reproduction |
title_short |
In vivo storage of oocytes leads to lower survival, increased abnormalities and may affect the ploidy status in the yellowtail tetra Astyanax altiparanae |
title_full |
In vivo storage of oocytes leads to lower survival, increased abnormalities and may affect the ploidy status in the yellowtail tetra Astyanax altiparanae |
title_fullStr |
In vivo storage of oocytes leads to lower survival, increased abnormalities and may affect the ploidy status in the yellowtail tetra Astyanax altiparanae |
title_full_unstemmed |
In vivo storage of oocytes leads to lower survival, increased abnormalities and may affect the ploidy status in the yellowtail tetra Astyanax altiparanae |
title_sort |
In vivo storage of oocytes leads to lower survival, increased abnormalities and may affect the ploidy status in the yellowtail tetra Astyanax altiparanae |
author |
Nascimento, Nivaldo Ferreira do [UNESP] |
author_facet |
Nascimento, Nivaldo Ferreira do [UNESP] Lazaro, Talita Maria [UNESP] Alcantara, Nathalia Raissa de [UNESP] Rocha Senhorini, Jose Augusto Santos, Silvio Carlos Alves dos Nakaghi, Laura Satiko Okada [UNESP] Yasui, George Shigueki |
author_role |
author |
author2 |
Lazaro, Talita Maria [UNESP] Alcantara, Nathalia Raissa de [UNESP] Rocha Senhorini, Jose Augusto Santos, Silvio Carlos Alves dos Nakaghi, Laura Satiko Okada [UNESP] Yasui, George Shigueki |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Chico Mendes Inst Biodivers Conservat AES Tiete |
dc.contributor.author.fl_str_mv |
Nascimento, Nivaldo Ferreira do [UNESP] Lazaro, Talita Maria [UNESP] Alcantara, Nathalia Raissa de [UNESP] Rocha Senhorini, Jose Augusto Santos, Silvio Carlos Alves dos Nakaghi, Laura Satiko Okada [UNESP] Yasui, George Shigueki |
dc.subject.por.fl_str_mv |
Ageing of oocytes Natural triploid Over-ripening Polyploidy, Reproduction |
topic |
Ageing of oocytes Natural triploid Over-ripening Polyploidy, Reproduction |
description |
In this study we analyzed whether the in vivo storage of oocytes (time after ovulation until fertilization) affects the survival and the ploidy status of the yellowtail tetra Astyanax altiparanae. Fish were induced to spawn and, after ovulation, a small aliquot was stripped and immediately fertilized (positive control group). Subsequently, aliquots (similar to 150 oocytes) were stripped and fertilized at various time points of 60, 120, 180 or 240 min. Developmental stages, abnormalities, survival and the ploidy status of the hatched larvae were examined. As expected, in the control group, 100% of the larvae were diploid. Conversely, triploid individuals were observed just at the 60 min treatment time point (0.6%). In vivo storage of oocytes also influenced the survival rates (P < 0.05); the 180 and 240 min samples, respectively, presented lower survival rates at gastrula (50.10 +/- 6.26% and 40.92 +/- 5.32%), and somite (17.80 +/- 5.14% and 4.41 +/- 2.76%) stages and lower hatching rates (12.01 +/- 4.04% and 4.41 +/- 2.76%). A higher percentage (99.27 +/- 0.40%) of normal larvae and only a few abnormal larvae (0.73 +/- 0.40%) were observed in the control group (P = 0.0000). This observation did not differ from that observed at the 60 min treatment point (P = 0.9976). A significant increase in the percentage of abnormalities was observed in the other treatments, and, after 240 min, the highest percentage of abnormal larvae was seen (P=0.0024; 83.33 +/- 16.67%). In conclusion, we showed that oocyte ageing had a significant effect on survival and may affect the ploidy status in A. atiparanae. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-01 2019-10-04T12:36:02Z 2019-10-04T12:36:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1017/S0967199418000527 Zygote. New York: Cambridge Univ Press, v. 26, n. 6, p. 471-475, 2018. 0967-1994 http://hdl.handle.net/11449/185505 10.1017/S0967199418000527 WOS:000460557500006 |
url |
http://dx.doi.org/10.1017/S0967199418000527 http://hdl.handle.net/11449/185505 |
identifier_str_mv |
Zygote. New York: Cambridge Univ Press, v. 26, n. 6, p. 471-475, 2018. 0967-1994 10.1017/S0967199418000527 WOS:000460557500006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Zygote |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
471-475 |
dc.publisher.none.fl_str_mv |
Cambridge Univ Press |
publisher.none.fl_str_mv |
Cambridge Univ Press |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803047039512084480 |