Short-term storage of the oocytes affects the ploidy status in the yellowtail tetra Astyanax altiparanae
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1017/S0967199417000739 http://hdl.handle.net/11449/163802 |
Resumo: | In fish, many factors can affect reproduction during in vitro fertilization, therefore determination of the factors that affect affecting gamete quality is needed. However, few studies have focused on gamete quality and the ploidy status. This study was conducted to elucidate whether oocyte storage can affect ploidy status, survival, and embryo viability in the characid species Astyanax altiparanae. Oocytes were stored in Dulbecco's phosphate-buffered saline (PBS) at 26 degrees C, then aliquots were fertilized immediately after extrusion (control) and also after 60, 120, 180, and 240 min of storage. Fertilization and hatching rates were measured, and the developmental stages were analyzed at each stage before describing the main abnormalities. Ploidy status was analyzed by flow cytometry and blood smear. In the control group, 100% of the samples were diploid. After treatment for 60 min, 95.56 +/- 4.44% samples were diploid and 4.44 +/- 4.44% were triploid. After 120 min, 94.44 +/- 9.62% of the samples was diploid and 5.56 +/- 5.56% were triploid; 100% of the samples were diploid after 180 min and, after 240 min, there was no survival. In other treatments, the highest percentage of hatching was after 60 min (88.93 +/- 5.15%; P = 0.015), and treatment with 180 min storage resulted in the highest percentage of abnormal larvae (95.76 +/- 12.67%; P = 0.012). These results show that oocyte storage can affect ploidy status and may be an interesting parameter for analysis in studies on chromosome set manipulation and micromanipulation. |
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Repositório Institucional da UNESP |
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Short-term storage of the oocytes affects the ploidy status in the yellowtail tetra Astyanax altiparanaeEmbryogenesisGametesMorphologyPolyploidyReproductionIn fish, many factors can affect reproduction during in vitro fertilization, therefore determination of the factors that affect affecting gamete quality is needed. However, few studies have focused on gamete quality and the ploidy status. This study was conducted to elucidate whether oocyte storage can affect ploidy status, survival, and embryo viability in the characid species Astyanax altiparanae. Oocytes were stored in Dulbecco's phosphate-buffered saline (PBS) at 26 degrees C, then aliquots were fertilized immediately after extrusion (control) and also after 60, 120, 180, and 240 min of storage. Fertilization and hatching rates were measured, and the developmental stages were analyzed at each stage before describing the main abnormalities. Ploidy status was analyzed by flow cytometry and blood smear. In the control group, 100% of the samples were diploid. After treatment for 60 min, 95.56 +/- 4.44% samples were diploid and 4.44 +/- 4.44% were triploid. After 120 min, 94.44 +/- 9.62% of the samples was diploid and 5.56 +/- 5.56% were triploid; 100% of the samples were diploid after 180 min and, after 240 min, there was no survival. In other treatments, the highest percentage of hatching was after 60 min (88.93 +/- 5.15%; P = 0.015), and treatment with 180 min storage resulted in the highest percentage of abnormal larvae (95.76 +/- 12.67%; P = 0.012). These results show that oocyte storage can affect ploidy status and may be an interesting parameter for analysis in studies on chromosome set manipulation and micromanipulation.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Sao Paulo State Univ, Aquaculture Ctr, Jaboticabal, BrazilNatl Ctr Res & Conservat Continental Fish, Chico Mendes Inst Biodivers Conservat, Pirassununga, BrazilHokkaido Univ, Fac Fisheries Sci, Hakodate, Hokkaido, JapanSao Paulo State Univ, Aquaculture Ctr, Jaboticabal, BrazilFAPESP: 2010/17429-1Cambridge Univ PressUniversidade Estadual Paulista (Unesp)Natl Ctr Res & Conservat Continental FishHokkaido UnivSantos, Matheus Pereira dos [UNESP]Nascimento, Nivaldo Ferreira do [UNESP]Yasui, George ShiguekiPereira, Nycolas Levy [UNESP]Fujimoto, TakafumiSenhorini, Jose AugustoOkada Nakaghi, Laura Satiko [UNESP]2018-11-26T17:45:02Z2018-11-26T17:45:02Z2018-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article89-98application/pdfhttp://dx.doi.org/10.1017/S0967199417000739Zygote. New York: Cambridge Univ Press, v. 26, n. 1, p. 89-98, 2018.0967-1994http://hdl.handle.net/11449/16380210.1017/S0967199417000739WOS:000424128200009WOS000424128200009.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengZygote0,387info:eu-repo/semantics/openAccess2024-04-09T15:37:02Zoai:repositorio.unesp.br:11449/163802Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:57:03.214444Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Short-term storage of the oocytes affects the ploidy status in the yellowtail tetra Astyanax altiparanae |
title |
Short-term storage of the oocytes affects the ploidy status in the yellowtail tetra Astyanax altiparanae |
spellingShingle |
Short-term storage of the oocytes affects the ploidy status in the yellowtail tetra Astyanax altiparanae Santos, Matheus Pereira dos [UNESP] Embryogenesis Gametes Morphology Polyploidy Reproduction |
title_short |
Short-term storage of the oocytes affects the ploidy status in the yellowtail tetra Astyanax altiparanae |
title_full |
Short-term storage of the oocytes affects the ploidy status in the yellowtail tetra Astyanax altiparanae |
title_fullStr |
Short-term storage of the oocytes affects the ploidy status in the yellowtail tetra Astyanax altiparanae |
title_full_unstemmed |
Short-term storage of the oocytes affects the ploidy status in the yellowtail tetra Astyanax altiparanae |
title_sort |
Short-term storage of the oocytes affects the ploidy status in the yellowtail tetra Astyanax altiparanae |
author |
Santos, Matheus Pereira dos [UNESP] |
author_facet |
Santos, Matheus Pereira dos [UNESP] Nascimento, Nivaldo Ferreira do [UNESP] Yasui, George Shigueki Pereira, Nycolas Levy [UNESP] Fujimoto, Takafumi Senhorini, Jose Augusto Okada Nakaghi, Laura Satiko [UNESP] |
author_role |
author |
author2 |
Nascimento, Nivaldo Ferreira do [UNESP] Yasui, George Shigueki Pereira, Nycolas Levy [UNESP] Fujimoto, Takafumi Senhorini, Jose Augusto Okada Nakaghi, Laura Satiko [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Natl Ctr Res & Conservat Continental Fish Hokkaido Univ |
dc.contributor.author.fl_str_mv |
Santos, Matheus Pereira dos [UNESP] Nascimento, Nivaldo Ferreira do [UNESP] Yasui, George Shigueki Pereira, Nycolas Levy [UNESP] Fujimoto, Takafumi Senhorini, Jose Augusto Okada Nakaghi, Laura Satiko [UNESP] |
dc.subject.por.fl_str_mv |
Embryogenesis Gametes Morphology Polyploidy Reproduction |
topic |
Embryogenesis Gametes Morphology Polyploidy Reproduction |
description |
In fish, many factors can affect reproduction during in vitro fertilization, therefore determination of the factors that affect affecting gamete quality is needed. However, few studies have focused on gamete quality and the ploidy status. This study was conducted to elucidate whether oocyte storage can affect ploidy status, survival, and embryo viability in the characid species Astyanax altiparanae. Oocytes were stored in Dulbecco's phosphate-buffered saline (PBS) at 26 degrees C, then aliquots were fertilized immediately after extrusion (control) and also after 60, 120, 180, and 240 min of storage. Fertilization and hatching rates were measured, and the developmental stages were analyzed at each stage before describing the main abnormalities. Ploidy status was analyzed by flow cytometry and blood smear. In the control group, 100% of the samples were diploid. After treatment for 60 min, 95.56 +/- 4.44% samples were diploid and 4.44 +/- 4.44% were triploid. After 120 min, 94.44 +/- 9.62% of the samples was diploid and 5.56 +/- 5.56% were triploid; 100% of the samples were diploid after 180 min and, after 240 min, there was no survival. In other treatments, the highest percentage of hatching was after 60 min (88.93 +/- 5.15%; P = 0.015), and treatment with 180 min storage resulted in the highest percentage of abnormal larvae (95.76 +/- 12.67%; P = 0.012). These results show that oocyte storage can affect ploidy status and may be an interesting parameter for analysis in studies on chromosome set manipulation and micromanipulation. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-26T17:45:02Z 2018-11-26T17:45:02Z 2018-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1017/S0967199417000739 Zygote. New York: Cambridge Univ Press, v. 26, n. 1, p. 89-98, 2018. 0967-1994 http://hdl.handle.net/11449/163802 10.1017/S0967199417000739 WOS:000424128200009 WOS000424128200009.pdf |
url |
http://dx.doi.org/10.1017/S0967199417000739 http://hdl.handle.net/11449/163802 |
identifier_str_mv |
Zygote. New York: Cambridge Univ Press, v. 26, n. 1, p. 89-98, 2018. 0967-1994 10.1017/S0967199417000739 WOS:000424128200009 WOS000424128200009.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Zygote 0,387 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
89-98 application/pdf |
dc.publisher.none.fl_str_mv |
Cambridge Univ Press |
publisher.none.fl_str_mv |
Cambridge Univ Press |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129265860870144 |