In vitro identification of a stem cell population from canine hair follicle bulge region

Detalhes bibliográficos
Autor(a) principal: Castro, Raquel V. G. de [UNESP]
Data de Publicação: 2018
Outros Autores: Tavares, Mariana R. [UNESP], Bressan, Fabiana F., Pieri, Naira C. G., Trindade Hill, Amanda Baracho [UNESP], Souza, Aline F., Cruz, Nathan da R. N. [UNESP], Martins, Daniele S., Ambrosio, Carlos E., Meirelles, Flavio V., Garcia, Joaquim M. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.tice.2017.12.003
http://hdl.handle.net/11449/163977
Resumo: Skin is an extensive and easily accessible organ possessing various cell types that are constantly renewed. Previous studies have suggested the presence of a stem cell niche at the bulge region of the hair follicle, which contains cells positive for CD200 and CD34. Thus, this study sought to identify these cell populations in canine skin cells using the following methods 1- collecting samples of adult and fetal skin and isolating and culturing these cells using a method of simple enzymatic digestion and 2-testing the cell cultures for CD200 and CD34 in vitro and comparing them with skin tissue samples (in situ). Immunofluorescence results were negative for both CD200 and CD34 in frozen and paraffin embedded tissue, whereas the analysis showed that cultured cells positive for CD34, CD200 and double positive cells could be visualized in different percentages. Additionally, the pluripotency marker OCT4 was positive in the isolated cells. Analysis of CD34, CD200 and OCT4 by RT-qPCR showed that there is expression in fetal and adult cells, although no difference was observed between groups. Our results suggest that bulge stem cells from both fetuses and adult dogs were reported with the use of CD34 and CD200 markers in this study, and further techniques for cell isolation and in vitro cultivation are needed in order to obtain enriched populations of skin stem cells in dogs.
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spelling In vitro identification of a stem cell population from canine hair follicle bulge regionSkinOCT4CD200CD34DogSkin is an extensive and easily accessible organ possessing various cell types that are constantly renewed. Previous studies have suggested the presence of a stem cell niche at the bulge region of the hair follicle, which contains cells positive for CD200 and CD34. Thus, this study sought to identify these cell populations in canine skin cells using the following methods 1- collecting samples of adult and fetal skin and isolating and culturing these cells using a method of simple enzymatic digestion and 2-testing the cell cultures for CD200 and CD34 in vitro and comparing them with skin tissue samples (in situ). Immunofluorescence results were negative for both CD200 and CD34 in frozen and paraffin embedded tissue, whereas the analysis showed that cultured cells positive for CD34, CD200 and double positive cells could be visualized in different percentages. Additionally, the pluripotency marker OCT4 was positive in the isolated cells. Analysis of CD34, CD200 and OCT4 by RT-qPCR showed that there is expression in fetal and adult cells, although no difference was observed between groups. Our results suggest that bulge stem cells from both fetuses and adult dogs were reported with the use of CD34 and CD200 markers in this study, and further techniques for cell isolation and in vitro cultivation are needed in order to obtain enriched populations of skin stem cells in dogs.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ, Fac Agr & Vet Sci, Dept Prevent Vet Med & Anim Reprod, Jaboticabal, SP, BrazilUniv Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Pirassununga, SP, BrazilUniv Sao Paulo, Fac Vet Med & Anim Sci, Dept Surg, Sao Paulo, SP, BrazilUniv Sao Paulo, Fac Vet Med & Anim Sci, Dept Anim Reprod, Sao Paulo, SP, BrazilSao Paulo State Univ, Fac Agr & Vet Sci, Dept Vet Clin & Surg, Jaboticabal, SP, BrazilSao Paulo State Univ, Fac Agr & Vet Sci, Dept Prevent Vet Med & Anim Reprod, Jaboticabal, SP, BrazilSao Paulo State Univ, Fac Agr & Vet Sci, Dept Vet Clin & Surg, Jaboticabal, SP, BrazilFAPESP: 2015/26818-5Churchill LivingstoneUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Castro, Raquel V. G. de [UNESP]Tavares, Mariana R. [UNESP]Bressan, Fabiana F.Pieri, Naira C. G.Trindade Hill, Amanda Baracho [UNESP]Souza, Aline F.Cruz, Nathan da R. N. [UNESP]Martins, Daniele S.Ambrosio, Carlos E.Meirelles, Flavio V.Garcia, Joaquim M. [UNESP]2018-11-26T17:48:38Z2018-11-26T17:48:38Z2018-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article43-50application/pdfhttp://dx.doi.org/10.1016/j.tice.2017.12.003Tissue & Cell. Edinburgh: Churchill Livingstone, v. 50, p. 43-50, 2018.0040-8166http://hdl.handle.net/11449/16397710.1016/j.tice.2017.12.003WOS:000427074600006WOS000427074600006.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTissue & Cell0,410info:eu-repo/semantics/openAccess2024-01-06T06:25:42Zoai:repositorio.unesp.br:11449/163977Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-06T06:25:42Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv In vitro identification of a stem cell population from canine hair follicle bulge region
title In vitro identification of a stem cell population from canine hair follicle bulge region
spellingShingle In vitro identification of a stem cell population from canine hair follicle bulge region
Castro, Raquel V. G. de [UNESP]
Skin
OCT4
CD200
CD34
Dog
title_short In vitro identification of a stem cell population from canine hair follicle bulge region
title_full In vitro identification of a stem cell population from canine hair follicle bulge region
title_fullStr In vitro identification of a stem cell population from canine hair follicle bulge region
title_full_unstemmed In vitro identification of a stem cell population from canine hair follicle bulge region
title_sort In vitro identification of a stem cell population from canine hair follicle bulge region
author Castro, Raquel V. G. de [UNESP]
author_facet Castro, Raquel V. G. de [UNESP]
Tavares, Mariana R. [UNESP]
Bressan, Fabiana F.
Pieri, Naira C. G.
Trindade Hill, Amanda Baracho [UNESP]
Souza, Aline F.
Cruz, Nathan da R. N. [UNESP]
Martins, Daniele S.
Ambrosio, Carlos E.
Meirelles, Flavio V.
Garcia, Joaquim M. [UNESP]
author_role author
author2 Tavares, Mariana R. [UNESP]
Bressan, Fabiana F.
Pieri, Naira C. G.
Trindade Hill, Amanda Baracho [UNESP]
Souza, Aline F.
Cruz, Nathan da R. N. [UNESP]
Martins, Daniele S.
Ambrosio, Carlos E.
Meirelles, Flavio V.
Garcia, Joaquim M. [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Castro, Raquel V. G. de [UNESP]
Tavares, Mariana R. [UNESP]
Bressan, Fabiana F.
Pieri, Naira C. G.
Trindade Hill, Amanda Baracho [UNESP]
Souza, Aline F.
Cruz, Nathan da R. N. [UNESP]
Martins, Daniele S.
Ambrosio, Carlos E.
Meirelles, Flavio V.
Garcia, Joaquim M. [UNESP]
dc.subject.por.fl_str_mv Skin
OCT4
CD200
CD34
Dog
topic Skin
OCT4
CD200
CD34
Dog
description Skin is an extensive and easily accessible organ possessing various cell types that are constantly renewed. Previous studies have suggested the presence of a stem cell niche at the bulge region of the hair follicle, which contains cells positive for CD200 and CD34. Thus, this study sought to identify these cell populations in canine skin cells using the following methods 1- collecting samples of adult and fetal skin and isolating and culturing these cells using a method of simple enzymatic digestion and 2-testing the cell cultures for CD200 and CD34 in vitro and comparing them with skin tissue samples (in situ). Immunofluorescence results were negative for both CD200 and CD34 in frozen and paraffin embedded tissue, whereas the analysis showed that cultured cells positive for CD34, CD200 and double positive cells could be visualized in different percentages. Additionally, the pluripotency marker OCT4 was positive in the isolated cells. Analysis of CD34, CD200 and OCT4 by RT-qPCR showed that there is expression in fetal and adult cells, although no difference was observed between groups. Our results suggest that bulge stem cells from both fetuses and adult dogs were reported with the use of CD34 and CD200 markers in this study, and further techniques for cell isolation and in vitro cultivation are needed in order to obtain enriched populations of skin stem cells in dogs.
publishDate 2018
dc.date.none.fl_str_mv 2018-11-26T17:48:38Z
2018-11-26T17:48:38Z
2018-02-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.tice.2017.12.003
Tissue & Cell. Edinburgh: Churchill Livingstone, v. 50, p. 43-50, 2018.
0040-8166
http://hdl.handle.net/11449/163977
10.1016/j.tice.2017.12.003
WOS:000427074600006
WOS000427074600006.pdf
url http://dx.doi.org/10.1016/j.tice.2017.12.003
http://hdl.handle.net/11449/163977
identifier_str_mv Tissue & Cell. Edinburgh: Churchill Livingstone, v. 50, p. 43-50, 2018.
0040-8166
10.1016/j.tice.2017.12.003
WOS:000427074600006
WOS000427074600006.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Tissue & Cell
0,410
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 43-50
application/pdf
dc.publisher.none.fl_str_mv Churchill Livingstone
publisher.none.fl_str_mv Churchill Livingstone
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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