In vitro identification of a stem cell population from canine hair follicle bulge region
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.tice.2017.12.003 http://hdl.handle.net/11449/163977 |
Resumo: | Skin is an extensive and easily accessible organ possessing various cell types that are constantly renewed. Previous studies have suggested the presence of a stem cell niche at the bulge region of the hair follicle, which contains cells positive for CD200 and CD34. Thus, this study sought to identify these cell populations in canine skin cells using the following methods 1- collecting samples of adult and fetal skin and isolating and culturing these cells using a method of simple enzymatic digestion and 2-testing the cell cultures for CD200 and CD34 in vitro and comparing them with skin tissue samples (in situ). Immunofluorescence results were negative for both CD200 and CD34 in frozen and paraffin embedded tissue, whereas the analysis showed that cultured cells positive for CD34, CD200 and double positive cells could be visualized in different percentages. Additionally, the pluripotency marker OCT4 was positive in the isolated cells. Analysis of CD34, CD200 and OCT4 by RT-qPCR showed that there is expression in fetal and adult cells, although no difference was observed between groups. Our results suggest that bulge stem cells from both fetuses and adult dogs were reported with the use of CD34 and CD200 markers in this study, and further techniques for cell isolation and in vitro cultivation are needed in order to obtain enriched populations of skin stem cells in dogs. |
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In vitro identification of a stem cell population from canine hair follicle bulge regionSkinOCT4CD200CD34DogSkin is an extensive and easily accessible organ possessing various cell types that are constantly renewed. Previous studies have suggested the presence of a stem cell niche at the bulge region of the hair follicle, which contains cells positive for CD200 and CD34. Thus, this study sought to identify these cell populations in canine skin cells using the following methods 1- collecting samples of adult and fetal skin and isolating and culturing these cells using a method of simple enzymatic digestion and 2-testing the cell cultures for CD200 and CD34 in vitro and comparing them with skin tissue samples (in situ). Immunofluorescence results were negative for both CD200 and CD34 in frozen and paraffin embedded tissue, whereas the analysis showed that cultured cells positive for CD34, CD200 and double positive cells could be visualized in different percentages. Additionally, the pluripotency marker OCT4 was positive in the isolated cells. Analysis of CD34, CD200 and OCT4 by RT-qPCR showed that there is expression in fetal and adult cells, although no difference was observed between groups. Our results suggest that bulge stem cells from both fetuses and adult dogs were reported with the use of CD34 and CD200 markers in this study, and further techniques for cell isolation and in vitro cultivation are needed in order to obtain enriched populations of skin stem cells in dogs.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ, Fac Agr & Vet Sci, Dept Prevent Vet Med & Anim Reprod, Jaboticabal, SP, BrazilUniv Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Pirassununga, SP, BrazilUniv Sao Paulo, Fac Vet Med & Anim Sci, Dept Surg, Sao Paulo, SP, BrazilUniv Sao Paulo, Fac Vet Med & Anim Sci, Dept Anim Reprod, Sao Paulo, SP, BrazilSao Paulo State Univ, Fac Agr & Vet Sci, Dept Vet Clin & Surg, Jaboticabal, SP, BrazilSao Paulo State Univ, Fac Agr & Vet Sci, Dept Prevent Vet Med & Anim Reprod, Jaboticabal, SP, BrazilSao Paulo State Univ, Fac Agr & Vet Sci, Dept Vet Clin & Surg, Jaboticabal, SP, BrazilFAPESP: 2015/26818-5Churchill LivingstoneUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Castro, Raquel V. G. de [UNESP]Tavares, Mariana R. [UNESP]Bressan, Fabiana F.Pieri, Naira C. G.Trindade Hill, Amanda Baracho [UNESP]Souza, Aline F.Cruz, Nathan da R. N. [UNESP]Martins, Daniele S.Ambrosio, Carlos E.Meirelles, Flavio V.Garcia, Joaquim M. [UNESP]2018-11-26T17:48:38Z2018-11-26T17:48:38Z2018-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article43-50application/pdfhttp://dx.doi.org/10.1016/j.tice.2017.12.003Tissue & Cell. Edinburgh: Churchill Livingstone, v. 50, p. 43-50, 2018.0040-8166http://hdl.handle.net/11449/16397710.1016/j.tice.2017.12.003WOS:000427074600006WOS000427074600006.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTissue & Cell0,410info:eu-repo/semantics/openAccess2024-06-06T18:10:12Zoai:repositorio.unesp.br:11449/163977Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:15:01.781669Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In vitro identification of a stem cell population from canine hair follicle bulge region |
title |
In vitro identification of a stem cell population from canine hair follicle bulge region |
spellingShingle |
In vitro identification of a stem cell population from canine hair follicle bulge region Castro, Raquel V. G. de [UNESP] Skin OCT4 CD200 CD34 Dog |
title_short |
In vitro identification of a stem cell population from canine hair follicle bulge region |
title_full |
In vitro identification of a stem cell population from canine hair follicle bulge region |
title_fullStr |
In vitro identification of a stem cell population from canine hair follicle bulge region |
title_full_unstemmed |
In vitro identification of a stem cell population from canine hair follicle bulge region |
title_sort |
In vitro identification of a stem cell population from canine hair follicle bulge region |
author |
Castro, Raquel V. G. de [UNESP] |
author_facet |
Castro, Raquel V. G. de [UNESP] Tavares, Mariana R. [UNESP] Bressan, Fabiana F. Pieri, Naira C. G. Trindade Hill, Amanda Baracho [UNESP] Souza, Aline F. Cruz, Nathan da R. N. [UNESP] Martins, Daniele S. Ambrosio, Carlos E. Meirelles, Flavio V. Garcia, Joaquim M. [UNESP] |
author_role |
author |
author2 |
Tavares, Mariana R. [UNESP] Bressan, Fabiana F. Pieri, Naira C. G. Trindade Hill, Amanda Baracho [UNESP] Souza, Aline F. Cruz, Nathan da R. N. [UNESP] Martins, Daniele S. Ambrosio, Carlos E. Meirelles, Flavio V. Garcia, Joaquim M. [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Castro, Raquel V. G. de [UNESP] Tavares, Mariana R. [UNESP] Bressan, Fabiana F. Pieri, Naira C. G. Trindade Hill, Amanda Baracho [UNESP] Souza, Aline F. Cruz, Nathan da R. N. [UNESP] Martins, Daniele S. Ambrosio, Carlos E. Meirelles, Flavio V. Garcia, Joaquim M. [UNESP] |
dc.subject.por.fl_str_mv |
Skin OCT4 CD200 CD34 Dog |
topic |
Skin OCT4 CD200 CD34 Dog |
description |
Skin is an extensive and easily accessible organ possessing various cell types that are constantly renewed. Previous studies have suggested the presence of a stem cell niche at the bulge region of the hair follicle, which contains cells positive for CD200 and CD34. Thus, this study sought to identify these cell populations in canine skin cells using the following methods 1- collecting samples of adult and fetal skin and isolating and culturing these cells using a method of simple enzymatic digestion and 2-testing the cell cultures for CD200 and CD34 in vitro and comparing them with skin tissue samples (in situ). Immunofluorescence results were negative for both CD200 and CD34 in frozen and paraffin embedded tissue, whereas the analysis showed that cultured cells positive for CD34, CD200 and double positive cells could be visualized in different percentages. Additionally, the pluripotency marker OCT4 was positive in the isolated cells. Analysis of CD34, CD200 and OCT4 by RT-qPCR showed that there is expression in fetal and adult cells, although no difference was observed between groups. Our results suggest that bulge stem cells from both fetuses and adult dogs were reported with the use of CD34 and CD200 markers in this study, and further techniques for cell isolation and in vitro cultivation are needed in order to obtain enriched populations of skin stem cells in dogs. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-26T17:48:38Z 2018-11-26T17:48:38Z 2018-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.tice.2017.12.003 Tissue & Cell. Edinburgh: Churchill Livingstone, v. 50, p. 43-50, 2018. 0040-8166 http://hdl.handle.net/11449/163977 10.1016/j.tice.2017.12.003 WOS:000427074600006 WOS000427074600006.pdf |
url |
http://dx.doi.org/10.1016/j.tice.2017.12.003 http://hdl.handle.net/11449/163977 |
identifier_str_mv |
Tissue & Cell. Edinburgh: Churchill Livingstone, v. 50, p. 43-50, 2018. 0040-8166 10.1016/j.tice.2017.12.003 WOS:000427074600006 WOS000427074600006.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Tissue & Cell 0,410 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
43-50 application/pdf |
dc.publisher.none.fl_str_mv |
Churchill Livingstone |
publisher.none.fl_str_mv |
Churchill Livingstone |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129408994639872 |