Species delimitation of neotropical Characins (Stevardiinae): Implications for taxonomy of complex groups
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0216786 http://hdl.handle.net/11449/189213 |
Resumo: | Accurate species delimitation is crucial for studies of phylogeny, phylogeography, ecology, conservation and biogeography. The limits of species and genera in the Characidae family are controversial due to its uncertain phylogenetic relationships, high level of morphological homoplasy and the use of ambiguous morphological characters for descriptions. Here we establish species boundaries for Bryconamericus, Hemibrycon, Knodus and Eretmobrycon (Stevardiinae: Characidae), previously diagnosed with morphology, using three different barcoding approaches (GMYC, PTP, ABGD). Results revealed that species delimitation was successful by the use of a single-gene approach and by following a workflow in the context of integrative taxonomy, making evident problems and mistakes in the cataloging of Characidae species. Hence, it was possible to infer boundaries at genus level for clusters in the trees (GMYC and PTP) and automatic partitions (ABGD) which were consistent with some of recent taxonomic changes proposed in Characidae. We found that discordance cases between methods were linked to limitations of the methods and associated to putative species cluster closely related, some historically problematic in their diagnosis and identification. Furthermore, we suggested taxonomic changes and possibly new species, revealing a high degree of hidden diversity. Finally, we propose a workflow as a fast, accurate and objective way to delimit species from mitochondrial DNA sequences and to help clarify the classification of this group. |
id |
UNSP_51c3b6112766bbc2a77f7156fc7b71fb |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/189213 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Species delimitation of neotropical Characins (Stevardiinae): Implications for taxonomy of complex groupsAccurate species delimitation is crucial for studies of phylogeny, phylogeography, ecology, conservation and biogeography. The limits of species and genera in the Characidae family are controversial due to its uncertain phylogenetic relationships, high level of morphological homoplasy and the use of ambiguous morphological characters for descriptions. Here we establish species boundaries for Bryconamericus, Hemibrycon, Knodus and Eretmobrycon (Stevardiinae: Characidae), previously diagnosed with morphology, using three different barcoding approaches (GMYC, PTP, ABGD). Results revealed that species delimitation was successful by the use of a single-gene approach and by following a workflow in the context of integrative taxonomy, making evident problems and mistakes in the cataloging of Characidae species. Hence, it was possible to infer boundaries at genus level for clusters in the trees (GMYC and PTP) and automatic partitions (ABGD) which were consistent with some of recent taxonomic changes proposed in Characidae. We found that discordance cases between methods were linked to limitations of the methods and associated to putative species cluster closely related, some historically problematic in their diagnosis and identification. Furthermore, we suggested taxonomic changes and possibly new species, revealing a high degree of hidden diversity. Finally, we propose a workflow as a fast, accurate and objective way to delimit species from mitochondrial DNA sequences and to help clarify the classification of this group.Advanced Manufacturing OfficeVicerrectoría de Investigación, Universidad de Costa RicaFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)CYTED Ciencia y Tecnología para el DesarrolloGeneral Motors CorporationLaboratorio de Ictiología Unidad de Ecología y Sistemática (UNESIS) Departamento de Biología Facultad de Ciencias Pontificia Universidad JaverianaDepartamento de Morfologia Instituto de Biociências Universidade Estadual Paulista (UNESP) Campus de BotucatuUniversidade Santo Amaro Rua Prof. Enéas de Siqueira Neto Jardim das ImbuiasCentro de Ciências da Vida e da Natureza Universidade Federal da Integração Latino-Americana–UNILAPrograma de Biología Ambiental Facultad de Ciencias Naturales y Matemáticas Universidad de IbaguéDepartamento de Morfologia Instituto de Biociências Universidade Estadual Paulista (UNESP) Campus de BotucatuVicerrectoría de Investigación, Universidad de Costa Rica: 00006552FAPESP: 2010/17009-2CNPq: 306054/2006-0CYTED Ciencia y Tecnología para el Desarrollo: 567/2012Pontificia Universidad JaverianaUniversidade Estadual Paulista (Unesp)Jardim das ImbuiasUniversidade Federal da Integração Latino-Americana–UNILAUniversidad de IbaguéGarcía-Melo, Jorge E.Oliveira, Claudio [UNESP]Da Costa Silva, Guilherme José [UNESP]Ochoa-Orrego, Luz E. [UNESP]Garcia Pereira, Luiz HenriqueMaldonado-Ocampo, Javier A.2019-10-06T16:33:34Z2019-10-06T16:33:34Z2019-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1371/journal.pone.0216786PLoS ONE, v. 14, n. 6, 2019.1932-6203http://hdl.handle.net/11449/18921310.1371/journal.pone.02167862-s2.0-85066814458Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS ONEinfo:eu-repo/semantics/openAccess2021-10-23T00:00:23Zoai:repositorio.unesp.br:11449/189213Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:31:08.441145Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Species delimitation of neotropical Characins (Stevardiinae): Implications for taxonomy of complex groups |
title |
Species delimitation of neotropical Characins (Stevardiinae): Implications for taxonomy of complex groups |
spellingShingle |
Species delimitation of neotropical Characins (Stevardiinae): Implications for taxonomy of complex groups García-Melo, Jorge E. |
title_short |
Species delimitation of neotropical Characins (Stevardiinae): Implications for taxonomy of complex groups |
title_full |
Species delimitation of neotropical Characins (Stevardiinae): Implications for taxonomy of complex groups |
title_fullStr |
Species delimitation of neotropical Characins (Stevardiinae): Implications for taxonomy of complex groups |
title_full_unstemmed |
Species delimitation of neotropical Characins (Stevardiinae): Implications for taxonomy of complex groups |
title_sort |
Species delimitation of neotropical Characins (Stevardiinae): Implications for taxonomy of complex groups |
author |
García-Melo, Jorge E. |
author_facet |
García-Melo, Jorge E. Oliveira, Claudio [UNESP] Da Costa Silva, Guilherme José [UNESP] Ochoa-Orrego, Luz E. [UNESP] Garcia Pereira, Luiz Henrique Maldonado-Ocampo, Javier A. |
author_role |
author |
author2 |
Oliveira, Claudio [UNESP] Da Costa Silva, Guilherme José [UNESP] Ochoa-Orrego, Luz E. [UNESP] Garcia Pereira, Luiz Henrique Maldonado-Ocampo, Javier A. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Pontificia Universidad Javeriana Universidade Estadual Paulista (Unesp) Jardim das Imbuias Universidade Federal da Integração Latino-Americana–UNILA Universidad de Ibagué |
dc.contributor.author.fl_str_mv |
García-Melo, Jorge E. Oliveira, Claudio [UNESP] Da Costa Silva, Guilherme José [UNESP] Ochoa-Orrego, Luz E. [UNESP] Garcia Pereira, Luiz Henrique Maldonado-Ocampo, Javier A. |
description |
Accurate species delimitation is crucial for studies of phylogeny, phylogeography, ecology, conservation and biogeography. The limits of species and genera in the Characidae family are controversial due to its uncertain phylogenetic relationships, high level of morphological homoplasy and the use of ambiguous morphological characters for descriptions. Here we establish species boundaries for Bryconamericus, Hemibrycon, Knodus and Eretmobrycon (Stevardiinae: Characidae), previously diagnosed with morphology, using three different barcoding approaches (GMYC, PTP, ABGD). Results revealed that species delimitation was successful by the use of a single-gene approach and by following a workflow in the context of integrative taxonomy, making evident problems and mistakes in the cataloging of Characidae species. Hence, it was possible to infer boundaries at genus level for clusters in the trees (GMYC and PTP) and automatic partitions (ABGD) which were consistent with some of recent taxonomic changes proposed in Characidae. We found that discordance cases between methods were linked to limitations of the methods and associated to putative species cluster closely related, some historically problematic in their diagnosis and identification. Furthermore, we suggested taxonomic changes and possibly new species, revealing a high degree of hidden diversity. Finally, we propose a workflow as a fast, accurate and objective way to delimit species from mitochondrial DNA sequences and to help clarify the classification of this group. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:33:34Z 2019-10-06T16:33:34Z 2019-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0216786 PLoS ONE, v. 14, n. 6, 2019. 1932-6203 http://hdl.handle.net/11449/189213 10.1371/journal.pone.0216786 2-s2.0-85066814458 |
url |
http://dx.doi.org/10.1371/journal.pone.0216786 http://hdl.handle.net/11449/189213 |
identifier_str_mv |
PLoS ONE, v. 14, n. 6, 2019. 1932-6203 10.1371/journal.pone.0216786 2-s2.0-85066814458 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLoS ONE |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129434477133824 |