Genetic diversity of hepatozoon spp. In rodents from chile
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1984-29612021082 http://hdl.handle.net/11449/229892 |
Resumo: | This study aimed to investigate the genetic diversity of Hepatozoon spp. in rodents from Valdivia, Chile. A total of 74 rodents (synanthropic n=38; wild n=36) were trapped in Valdivia. We performed conventional PCR assays for Apicomplexa organisms targeting two overlapping 18S rDNA gene fragments (600 bp and 900 bp) followed by sequencing of selected amplicons. Hepatozoon spp. occurrence was 82.43% (61/74). Twelve sequences obtained from the 600 bp and ten from the 900 bp 18S rDNA fragments were identified as Hepatozoon sp. Six sequences obtained from 18S rDNA-based overlapping PCR protocols were used for concatenated (1,400 bp) phylogenetic, haplotype and distance analyses. Hepatozoon spp. 18S rDNA concatenated sequences from the present study were detected in Oligoryzomys longicaudatus, Rattus norvegicus, Mus musculus, and Abrothrix longipilis grouped with Hepatozoon species earlier described in rodents and reptiles from Chile and Brazil. Nucleotide polymorphism of the six 18S rDNA sequences (1,400 bp) from this study, and other Chilean sequences from rodents and rodent’s ticks, showed high diversity with a total of nine Chilean haplotypes. Three haplotypes from Valdivia were identified for the first time in this study, suggesting the circulation of novel haplotypes in rodents from southern Chile. |
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Genetic diversity of hepatozoon spp. In rodents from chileDiversidade genética de hepatozoon spp. Em roedores do chileApicomplexanHepatozoonosisPCRPhylogeneticsRodentiaSouth americaThis study aimed to investigate the genetic diversity of Hepatozoon spp. in rodents from Valdivia, Chile. A total of 74 rodents (synanthropic n=38; wild n=36) were trapped in Valdivia. We performed conventional PCR assays for Apicomplexa organisms targeting two overlapping 18S rDNA gene fragments (600 bp and 900 bp) followed by sequencing of selected amplicons. Hepatozoon spp. occurrence was 82.43% (61/74). Twelve sequences obtained from the 600 bp and ten from the 900 bp 18S rDNA fragments were identified as Hepatozoon sp. Six sequences obtained from 18S rDNA-based overlapping PCR protocols were used for concatenated (1,400 bp) phylogenetic, haplotype and distance analyses. Hepatozoon spp. 18S rDNA concatenated sequences from the present study were detected in Oligoryzomys longicaudatus, Rattus norvegicus, Mus musculus, and Abrothrix longipilis grouped with Hepatozoon species earlier described in rodents and reptiles from Chile and Brazil. Nucleotide polymorphism of the six 18S rDNA sequences (1,400 bp) from this study, and other Chilean sequences from rodents and rodent’s ticks, showed high diversity with a total of nine Chilean haplotypes. Three haplotypes from Valdivia were identified for the first time in this study, suggesting the circulation of novel haplotypes in rodents from southern Chile.Instituto de Ciencias Clínicas Veterinarias Facultad de Ciencias Veterinarias Universidad Austral de ChileInsituto de Medicina Preventiva Veterinaria Facultad de Ciencias Veterinarias Universidad Austral de ChileInstituto de Microbiologia Clínica Facultad de Medicina Universidad Austral de ChileDepartamento de Patologia Reprodução e Saúde Única Faculdade de Ciências Agrárias e Veterinarias – FCAV Universidade Estadual Paulista – UNESPOne Health Center for Zoonoses and Tropical Veterinary Medicine Department of Biomedical Sciences Ross University School of Veterinary MedicineDepartamento de Patologia Reprodução e Saúde Única Faculdade de Ciências Agrárias e Veterinarias – FCAV Universidade Estadual Paulista – UNESPUniversidad Austral de ChileUniversidade Estadual Paulista (UNESP)Ross University School of Veterinary MedicineAlabí, Amir SalvadorMonti, GustavoOtth, CarolaSepulveda-García, PaulinaPerles, Livia [UNESP]Machado, Rosangela Zacarias [UNESP]André, Marcos Rogério [UNESP]Bittencourt, PedroMüller, Ananda2022-04-29T08:36:23Z2022-04-29T08:36:23Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1590/S1984-29612021082Revista Brasileira de Parasitologia Veterinaria, v. 30, n. 4, 2021.1984-29610103-846Xhttp://hdl.handle.net/11449/22989210.1590/S1984-296120210822-s2.0-85119126357Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Parasitologia Veterinariainfo:eu-repo/semantics/openAccess2024-06-07T13:02:26Zoai:repositorio.unesp.br:11449/229892Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:50:04.838272Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genetic diversity of hepatozoon spp. In rodents from chile Diversidade genética de hepatozoon spp. Em roedores do chile |
title |
Genetic diversity of hepatozoon spp. In rodents from chile |
spellingShingle |
Genetic diversity of hepatozoon spp. In rodents from chile Alabí, Amir Salvador Apicomplexan Hepatozoonosis PCR Phylogenetics Rodentia South america |
title_short |
Genetic diversity of hepatozoon spp. In rodents from chile |
title_full |
Genetic diversity of hepatozoon spp. In rodents from chile |
title_fullStr |
Genetic diversity of hepatozoon spp. In rodents from chile |
title_full_unstemmed |
Genetic diversity of hepatozoon spp. In rodents from chile |
title_sort |
Genetic diversity of hepatozoon spp. In rodents from chile |
author |
Alabí, Amir Salvador |
author_facet |
Alabí, Amir Salvador Monti, Gustavo Otth, Carola Sepulveda-García, Paulina Perles, Livia [UNESP] Machado, Rosangela Zacarias [UNESP] André, Marcos Rogério [UNESP] Bittencourt, Pedro Müller, Ananda |
author_role |
author |
author2 |
Monti, Gustavo Otth, Carola Sepulveda-García, Paulina Perles, Livia [UNESP] Machado, Rosangela Zacarias [UNESP] André, Marcos Rogério [UNESP] Bittencourt, Pedro Müller, Ananda |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidad Austral de Chile Universidade Estadual Paulista (UNESP) Ross University School of Veterinary Medicine |
dc.contributor.author.fl_str_mv |
Alabí, Amir Salvador Monti, Gustavo Otth, Carola Sepulveda-García, Paulina Perles, Livia [UNESP] Machado, Rosangela Zacarias [UNESP] André, Marcos Rogério [UNESP] Bittencourt, Pedro Müller, Ananda |
dc.subject.por.fl_str_mv |
Apicomplexan Hepatozoonosis PCR Phylogenetics Rodentia South america |
topic |
Apicomplexan Hepatozoonosis PCR Phylogenetics Rodentia South america |
description |
This study aimed to investigate the genetic diversity of Hepatozoon spp. in rodents from Valdivia, Chile. A total of 74 rodents (synanthropic n=38; wild n=36) were trapped in Valdivia. We performed conventional PCR assays for Apicomplexa organisms targeting two overlapping 18S rDNA gene fragments (600 bp and 900 bp) followed by sequencing of selected amplicons. Hepatozoon spp. occurrence was 82.43% (61/74). Twelve sequences obtained from the 600 bp and ten from the 900 bp 18S rDNA fragments were identified as Hepatozoon sp. Six sequences obtained from 18S rDNA-based overlapping PCR protocols were used for concatenated (1,400 bp) phylogenetic, haplotype and distance analyses. Hepatozoon spp. 18S rDNA concatenated sequences from the present study were detected in Oligoryzomys longicaudatus, Rattus norvegicus, Mus musculus, and Abrothrix longipilis grouped with Hepatozoon species earlier described in rodents and reptiles from Chile and Brazil. Nucleotide polymorphism of the six 18S rDNA sequences (1,400 bp) from this study, and other Chilean sequences from rodents and rodent’s ticks, showed high diversity with a total of nine Chilean haplotypes. Three haplotypes from Valdivia were identified for the first time in this study, suggesting the circulation of novel haplotypes in rodents from southern Chile. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 2022-04-29T08:36:23Z 2022-04-29T08:36:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1984-29612021082 Revista Brasileira de Parasitologia Veterinaria, v. 30, n. 4, 2021. 1984-2961 0103-846X http://hdl.handle.net/11449/229892 10.1590/S1984-29612021082 2-s2.0-85119126357 |
url |
http://dx.doi.org/10.1590/S1984-29612021082 http://hdl.handle.net/11449/229892 |
identifier_str_mv |
Revista Brasileira de Parasitologia Veterinaria, v. 30, n. 4, 2021. 1984-2961 0103-846X 10.1590/S1984-29612021082 2-s2.0-85119126357 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinaria |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128987411513344 |