Sex chromosomes and internal telomeric sequences in dormitator latifrons (Richardson 1844) (eleotridae: Eleotrinae): An insight into their origin in the genus

Detalhes bibliográficos
Autor(a) principal: Paim, Fabilene Gomes [UNESP]
Data de Publicação: 2020
Outros Autores: Nirchio, Mauro, Oliveira, Claudio [UNESP], Rossi, Anna Rita
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/genes11060659
http://hdl.handle.net/11449/199002
Resumo: The freshwater fish species Dormitator latifrons, commonly named the Pacific fat sleeper, is an important food resource in CentralSouth America, yet almost no genetic information on it is available. A cytogenetic analysis of this species was undertaken by standard and molecular techniques (chromosomal mapping of 18S rDNA, 5S rDNA, and telomeric repeats), aiming to describe the karyotype features, verify the presence of sex chromosomes described in congeneric species, and make inferences on chromosome evolution in the genus. The karyotype (2n = 46) is mainly composed of metacentric and submetacentic chromosomes, with nucleolar organizer regions (NORs) localized on the short arms of submetacentric pair 10. The presence of XX/XY sex chromosomes was observed, with the X chromosome carrying the 5S rDNA sequences. These heterochromosomes likely appeared before 1 million years ago, since they are shared with another derived Dormitator species (Dormitator maculatus) distributed in the Western Atlantic. Telomeric repeats hybridize to the terminal portions of almost all chromosomes; additional interstitial sites are present in the centromeric region, suggesting pericentromeric inversions as the main rearrangement mechanisms that has driven karyotypic evolution in the genus. The data provided here contribute to improving the cytogenetics knowledge of D. latifrons, offering basic information that could be useful in aquaculture farming of this neotropical fish.
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spelling Sex chromosomes and internal telomeric sequences in dormitator latifrons (Richardson 1844) (eleotridae: Eleotrinae): An insight into their origin in the genus(TTAGG)nFISHITSPacific fat sleeperRDNAXX/XY sex systemThe freshwater fish species Dormitator latifrons, commonly named the Pacific fat sleeper, is an important food resource in CentralSouth America, yet almost no genetic information on it is available. A cytogenetic analysis of this species was undertaken by standard and molecular techniques (chromosomal mapping of 18S rDNA, 5S rDNA, and telomeric repeats), aiming to describe the karyotype features, verify the presence of sex chromosomes described in congeneric species, and make inferences on chromosome evolution in the genus. The karyotype (2n = 46) is mainly composed of metacentric and submetacentic chromosomes, with nucleolar organizer regions (NORs) localized on the short arms of submetacentric pair 10. The presence of XX/XY sex chromosomes was observed, with the X chromosome carrying the 5S rDNA sequences. These heterochromosomes likely appeared before 1 million years ago, since they are shared with another derived Dormitator species (Dormitator maculatus) distributed in the Western Atlantic. Telomeric repeats hybridize to the terminal portions of almost all chromosomes; additional interstitial sites are present in the centromeric region, suggesting pericentromeric inversions as the main rearrangement mechanisms that has driven karyotypic evolution in the genus. The data provided here contribute to improving the cytogenetics knowledge of D. latifrons, offering basic information that could be useful in aquaculture farming of this neotropical fish.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Departamento de Biologia Estrutural e Funcional Instituto de Biociências Universidade Estadual Paulista-UNESPUniversidad Técnica de Machala, Av. Panamericana km 5½, Vía PasajeEscuela de Ciencias Aplicadas del Mar Núcleo de Nueva Esparta Universidad de OrienteDipartimento di Biologia e Biotecnologie “C. Darwin” Sapienza-Università di Roma, Via Alfonso Borelli 50Departamento de Biologia Estrutural e Funcional Instituto de Biociências Universidade Estadual Paulista-UNESPFAPESP: 2014/26508-3FAPESP: 2016/09204-6FAPESP: 2018/20610-1CNPq: 306054/2006-0Universidade Estadual Paulista (Unesp)Universidad Técnica de MachalaUniversidad de OrienteSapienza-Università di RomaPaim, Fabilene Gomes [UNESP]Nirchio, MauroOliveira, Claudio [UNESP]Rossi, Anna Rita2020-12-12T01:28:01Z2020-12-12T01:28:01Z2020-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-12http://dx.doi.org/10.3390/genes11060659Genes, v. 11, n. 6, p. 1-12, 2020.2073-4425http://hdl.handle.net/11449/19900210.3390/genes110606592-s2.0-85086644978Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenesinfo:eu-repo/semantics/openAccess2021-10-22T22:17:21Zoai:repositorio.unesp.br:11449/199002Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:11:22.186768Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Sex chromosomes and internal telomeric sequences in dormitator latifrons (Richardson 1844) (eleotridae: Eleotrinae): An insight into their origin in the genus
title Sex chromosomes and internal telomeric sequences in dormitator latifrons (Richardson 1844) (eleotridae: Eleotrinae): An insight into their origin in the genus
spellingShingle Sex chromosomes and internal telomeric sequences in dormitator latifrons (Richardson 1844) (eleotridae: Eleotrinae): An insight into their origin in the genus
Paim, Fabilene Gomes [UNESP]
(TTAGG)n
FISH
ITS
Pacific fat sleeper
RDNA
XX/XY sex system
title_short Sex chromosomes and internal telomeric sequences in dormitator latifrons (Richardson 1844) (eleotridae: Eleotrinae): An insight into their origin in the genus
title_full Sex chromosomes and internal telomeric sequences in dormitator latifrons (Richardson 1844) (eleotridae: Eleotrinae): An insight into their origin in the genus
title_fullStr Sex chromosomes and internal telomeric sequences in dormitator latifrons (Richardson 1844) (eleotridae: Eleotrinae): An insight into their origin in the genus
title_full_unstemmed Sex chromosomes and internal telomeric sequences in dormitator latifrons (Richardson 1844) (eleotridae: Eleotrinae): An insight into their origin in the genus
title_sort Sex chromosomes and internal telomeric sequences in dormitator latifrons (Richardson 1844) (eleotridae: Eleotrinae): An insight into their origin in the genus
author Paim, Fabilene Gomes [UNESP]
author_facet Paim, Fabilene Gomes [UNESP]
Nirchio, Mauro
Oliveira, Claudio [UNESP]
Rossi, Anna Rita
author_role author
author2 Nirchio, Mauro
Oliveira, Claudio [UNESP]
Rossi, Anna Rita
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidad Técnica de Machala
Universidad de Oriente
Sapienza-Università di Roma
dc.contributor.author.fl_str_mv Paim, Fabilene Gomes [UNESP]
Nirchio, Mauro
Oliveira, Claudio [UNESP]
Rossi, Anna Rita
dc.subject.por.fl_str_mv (TTAGG)n
FISH
ITS
Pacific fat sleeper
RDNA
XX/XY sex system
topic (TTAGG)n
FISH
ITS
Pacific fat sleeper
RDNA
XX/XY sex system
description The freshwater fish species Dormitator latifrons, commonly named the Pacific fat sleeper, is an important food resource in CentralSouth America, yet almost no genetic information on it is available. A cytogenetic analysis of this species was undertaken by standard and molecular techniques (chromosomal mapping of 18S rDNA, 5S rDNA, and telomeric repeats), aiming to describe the karyotype features, verify the presence of sex chromosomes described in congeneric species, and make inferences on chromosome evolution in the genus. The karyotype (2n = 46) is mainly composed of metacentric and submetacentic chromosomes, with nucleolar organizer regions (NORs) localized on the short arms of submetacentric pair 10. The presence of XX/XY sex chromosomes was observed, with the X chromosome carrying the 5S rDNA sequences. These heterochromosomes likely appeared before 1 million years ago, since they are shared with another derived Dormitator species (Dormitator maculatus) distributed in the Western Atlantic. Telomeric repeats hybridize to the terminal portions of almost all chromosomes; additional interstitial sites are present in the centromeric region, suggesting pericentromeric inversions as the main rearrangement mechanisms that has driven karyotypic evolution in the genus. The data provided here contribute to improving the cytogenetics knowledge of D. latifrons, offering basic information that could be useful in aquaculture farming of this neotropical fish.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T01:28:01Z
2020-12-12T01:28:01Z
2020-06-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/genes11060659
Genes, v. 11, n. 6, p. 1-12, 2020.
2073-4425
http://hdl.handle.net/11449/199002
10.3390/genes11060659
2-s2.0-85086644978
url http://dx.doi.org/10.3390/genes11060659
http://hdl.handle.net/11449/199002
identifier_str_mv Genes, v. 11, n. 6, p. 1-12, 2020.
2073-4425
10.3390/genes11060659
2-s2.0-85086644978
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Genes
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1-12
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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