Black-pigmented anaerobic bacteria associated with ovine periodontitis
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.vetmic.2017.03.032 http://hdl.handle.net/11449/165650 |
Resumo: | Periodontitis is a polymicrobial infectious disease that causes occlusion change, tooth loss, difficulty in rumination, and premature culling of animals. This study aimed to detect species of the genera Porphyromonas and Prevotella present in the periodontal pocket of sheep with lesions deeper than 5 mm (n = 14) and in the gingival sulcus of animals considered periodontally healthy (n = 20). The presence of microorganisms was evaluated by polymerase chain reaction (PCR) using specific primers for Porphyromonas asaccharolytica, Porphyromonas endodontalis, Porphyromonas gingivalis, Porphyromonas gulae, Prevotella buccae, Prevotella intermedia, Prevotella loescheii, Prevotella melaninogenica, Prevotella nigrescens, Prevotella oralis, and Prevotella tannerae. Prevalence and risk analysis were performed using Student's t-test and Spearman's correlation. Among the Prevotella and Porphyromonas species detected in the periodontal lesions of sheep, P. melaninogenica (85.7%), P. buccae (64.3%), P. gingivalis (50%), and P. endodontalis (50%) were most prevalent. P. gingivalis (15%) and P. oralis (10%) prevailed in the gingival sulcus. P. gulae and P. tannerae were not detected in the 34 samples studied. Data evaluation by t-test verified that occurrence of P. asaccharolytica, P. endodontalis, P. gingivalis, P. buccae, P. intermedia, P. melcdinogenica, and P. nigrescens correlated with sheep periodontitis. The findings of this study will be an important contribution to research on pathogenesis of sheep periodontitis and development of its control measures. |
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Black-pigmented anaerobic bacteria associated with ovine periodontitisPeriodontal diseasePeriodontitisSheepPrevotellaPorphyromonasPeriodontitis is a polymicrobial infectious disease that causes occlusion change, tooth loss, difficulty in rumination, and premature culling of animals. This study aimed to detect species of the genera Porphyromonas and Prevotella present in the periodontal pocket of sheep with lesions deeper than 5 mm (n = 14) and in the gingival sulcus of animals considered periodontally healthy (n = 20). The presence of microorganisms was evaluated by polymerase chain reaction (PCR) using specific primers for Porphyromonas asaccharolytica, Porphyromonas endodontalis, Porphyromonas gingivalis, Porphyromonas gulae, Prevotella buccae, Prevotella intermedia, Prevotella loescheii, Prevotella melaninogenica, Prevotella nigrescens, Prevotella oralis, and Prevotella tannerae. Prevalence and risk analysis were performed using Student's t-test and Spearman's correlation. Among the Prevotella and Porphyromonas species detected in the periodontal lesions of sheep, P. melaninogenica (85.7%), P. buccae (64.3%), P. gingivalis (50%), and P. endodontalis (50%) were most prevalent. P. gingivalis (15%) and P. oralis (10%) prevailed in the gingival sulcus. P. gulae and P. tannerae were not detected in the 34 samples studied. Data evaluation by t-test verified that occurrence of P. asaccharolytica, P. endodontalis, P. gingivalis, P. buccae, P. intermedia, P. melcdinogenica, and P. nigrescens correlated with sheep periodontitis. The findings of this study will be an important contribution to research on pathogenesis of sheep periodontitis and development of its control measures.Univ Estadual Paulista, Sch Vet Med, Aracatuba, BrazilUniv Estadual Paulista, Dent Sch, Aracatuba, BrazilUniv Estadual Paulista, Sch Engn, Ilha Solteira, BrazilUniv Glasgow, Sch Vet Med, Glasgow, Lanark, ScotlandUniv Glasgow, Dent Sch, Glasgow, Lanark, ScotlandUniv Estadual Paulista, Sch Vet Med, Aracatuba, BrazilUniv Estadual Paulista, Dent Sch, Aracatuba, BrazilUniv Estadual Paulista, Sch Engn, Ilha Solteira, BrazilElsevier B.V.Universidade Estadual Paulista (Unesp)Univ GlasgowBorsanelli, Ana C. [UNESP]Gaetti-Jardim, Elerson [UNESP]Schweitzer, Christiane M. [UNESP]Viora, LorenzoBusin, ValentinaRiggio, Marcello P.Dutra, Iveraldo S. [UNESP]2018-11-28T14:21:46Z2018-11-28T14:21:46Z2017-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article271-274application/pdfhttp://dx.doi.org/10.1016/j.vetmic.2017.03.032Veterinary Microbiology. Amsterdam: Elsevier Science Bv, v. 203, p. 271-274, 2017.0378-1135http://hdl.handle.net/11449/16565010.1016/j.vetmic.2017.03.032WOS:000404710300038WOS000404710300038.pdf00011553552103270000-0001-9561-8281Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Microbiology1,175info:eu-repo/semantics/openAccess2023-12-31T06:21:35Zoai:repositorio.unesp.br:11449/165650Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-31T06:21:35Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Black-pigmented anaerobic bacteria associated with ovine periodontitis |
title |
Black-pigmented anaerobic bacteria associated with ovine periodontitis |
spellingShingle |
Black-pigmented anaerobic bacteria associated with ovine periodontitis Borsanelli, Ana C. [UNESP] Periodontal disease Periodontitis Sheep Prevotella Porphyromonas |
title_short |
Black-pigmented anaerobic bacteria associated with ovine periodontitis |
title_full |
Black-pigmented anaerobic bacteria associated with ovine periodontitis |
title_fullStr |
Black-pigmented anaerobic bacteria associated with ovine periodontitis |
title_full_unstemmed |
Black-pigmented anaerobic bacteria associated with ovine periodontitis |
title_sort |
Black-pigmented anaerobic bacteria associated with ovine periodontitis |
author |
Borsanelli, Ana C. [UNESP] |
author_facet |
Borsanelli, Ana C. [UNESP] Gaetti-Jardim, Elerson [UNESP] Schweitzer, Christiane M. [UNESP] Viora, Lorenzo Busin, Valentina Riggio, Marcello P. Dutra, Iveraldo S. [UNESP] |
author_role |
author |
author2 |
Gaetti-Jardim, Elerson [UNESP] Schweitzer, Christiane M. [UNESP] Viora, Lorenzo Busin, Valentina Riggio, Marcello P. Dutra, Iveraldo S. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Glasgow |
dc.contributor.author.fl_str_mv |
Borsanelli, Ana C. [UNESP] Gaetti-Jardim, Elerson [UNESP] Schweitzer, Christiane M. [UNESP] Viora, Lorenzo Busin, Valentina Riggio, Marcello P. Dutra, Iveraldo S. [UNESP] |
dc.subject.por.fl_str_mv |
Periodontal disease Periodontitis Sheep Prevotella Porphyromonas |
topic |
Periodontal disease Periodontitis Sheep Prevotella Porphyromonas |
description |
Periodontitis is a polymicrobial infectious disease that causes occlusion change, tooth loss, difficulty in rumination, and premature culling of animals. This study aimed to detect species of the genera Porphyromonas and Prevotella present in the periodontal pocket of sheep with lesions deeper than 5 mm (n = 14) and in the gingival sulcus of animals considered periodontally healthy (n = 20). The presence of microorganisms was evaluated by polymerase chain reaction (PCR) using specific primers for Porphyromonas asaccharolytica, Porphyromonas endodontalis, Porphyromonas gingivalis, Porphyromonas gulae, Prevotella buccae, Prevotella intermedia, Prevotella loescheii, Prevotella melaninogenica, Prevotella nigrescens, Prevotella oralis, and Prevotella tannerae. Prevalence and risk analysis were performed using Student's t-test and Spearman's correlation. Among the Prevotella and Porphyromonas species detected in the periodontal lesions of sheep, P. melaninogenica (85.7%), P. buccae (64.3%), P. gingivalis (50%), and P. endodontalis (50%) were most prevalent. P. gingivalis (15%) and P. oralis (10%) prevailed in the gingival sulcus. P. gulae and P. tannerae were not detected in the 34 samples studied. Data evaluation by t-test verified that occurrence of P. asaccharolytica, P. endodontalis, P. gingivalis, P. buccae, P. intermedia, P. melcdinogenica, and P. nigrescens correlated with sheep periodontitis. The findings of this study will be an important contribution to research on pathogenesis of sheep periodontitis and development of its control measures. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-05-01 2018-11-28T14:21:46Z 2018-11-28T14:21:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.vetmic.2017.03.032 Veterinary Microbiology. Amsterdam: Elsevier Science Bv, v. 203, p. 271-274, 2017. 0378-1135 http://hdl.handle.net/11449/165650 10.1016/j.vetmic.2017.03.032 WOS:000404710300038 WOS000404710300038.pdf 0001155355210327 0000-0001-9561-8281 |
url |
http://dx.doi.org/10.1016/j.vetmic.2017.03.032 http://hdl.handle.net/11449/165650 |
identifier_str_mv |
Veterinary Microbiology. Amsterdam: Elsevier Science Bv, v. 203, p. 271-274, 2017. 0378-1135 10.1016/j.vetmic.2017.03.032 WOS:000404710300038 WOS000404710300038.pdf 0001155355210327 0000-0001-9561-8281 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Veterinary Microbiology 1,175 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
271-274 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965471753109504 |