Presence of Porphyromonas and Prevotella species in the oral microflora of cattle with periodontitis

Detalhes bibliográficos
Autor(a) principal: Borsanelli, Ana Carolina [UNESP]
Data de Publicação: 2015
Outros Autores: Júnior, Elerson Gaetti-Jardim [UNESP], Schweitzer, Christiane Marie [UNESP], Döbereiner, Jürgen, Dutra, Iveraldo S. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/s0100-736x2015001000002
http://hdl.handle.net/11449/172293
Resumo: Bovine periodontitis is a progressive purulent infectious process associated with the presence of strictly and facultative anaerobic subgingival biofilm and epidemiologically related to soil management in large geographic areas of Brazil. This study aimed to detect species of the genera Porphyromonas and Prevotella, which occurr in periodontal pockets of cattle with lesions deeper than 5mm (n=26) and in gingival sulcus of animals considered periodontally healthy (n=25). Presence of the microorganisms was evaluated by independent- culture medium diagnostic method, using polymerase chain reaction (PCR) with specific primers of Porphyromonas asaccharolytica, P. endodontalis, P. gingivalis, P. gulae, Prevotella buccae, P. intermedia, P. loescheii, P. melaninogenica, P. nigrescens, P. oralis and P. tannerae. The species P. endodontalis (80.7%), P. melaninogenica (73.1%) and P. intermedia (61.5%) were the most predominant in samples of cattle with periodontitis. Regarding non -injured gingival sulcus of cattle, P. endodontalis (40%) and P. loeschei (40%) prevailed. Porphyromonas gingivalis, P. gulae and Prevotella tannerae were not detected in the 51 samples studied. Data evaluation by T test, enabled to verify that ocorrence of Porphyromonas asaccharolytica (p=0.000003), P. endodontalis (p=0.0023), Prevotella buccae (p=0.0017), P. intermedia (p=0.0020), P. melaninogenica (p=0.00006) and P. oralis (p=0.0028) is correlated with bovine periodontitis.
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spelling Presence of Porphyromonas and Prevotella species in the oral microflora of cattle with periodontitisCattlePeriodontitisPorphyromonas spp.Prevotella spp.Bovine periodontitis is a progressive purulent infectious process associated with the presence of strictly and facultative anaerobic subgingival biofilm and epidemiologically related to soil management in large geographic areas of Brazil. This study aimed to detect species of the genera Porphyromonas and Prevotella, which occurr in periodontal pockets of cattle with lesions deeper than 5mm (n=26) and in gingival sulcus of animals considered periodontally healthy (n=25). Presence of the microorganisms was evaluated by independent- culture medium diagnostic method, using polymerase chain reaction (PCR) with specific primers of Porphyromonas asaccharolytica, P. endodontalis, P. gingivalis, P. gulae, Prevotella buccae, P. intermedia, P. loescheii, P. melaninogenica, P. nigrescens, P. oralis and P. tannerae. The species P. endodontalis (80.7%), P. melaninogenica (73.1%) and P. intermedia (61.5%) were the most predominant in samples of cattle with periodontitis. Regarding non -injured gingival sulcus of cattle, P. endodontalis (40%) and P. loeschei (40%) prevailed. Porphyromonas gingivalis, P. gulae and Prevotella tannerae were not detected in the 51 samples studied. Data evaluation by T test, enabled to verify that ocorrence of Porphyromonas asaccharolytica (p=0.000003), P. endodontalis (p=0.0023), Prevotella buccae (p=0.0017), P. intermedia (p=0.0020), P. melaninogenica (p=0.00006) and P. oralis (p=0.0028) is correlated with bovine periodontitis.Programa de Pós-Graduação em Medicina Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (Unesp), Via de Acesso Professor Paulo Donato Castellane s/nDepartamento de Patologia e Propedêutica Clínica Faculdade de Odontologia de Araçatuba Unesp, Rua José Bonifácio 1193Departamento de Matemática Faculdade de Engenharia de Ilha Solteira Unesp, Alameda Rio de Janeiro 266Ex-Pesquisador da Empresa Brasileira de Pesquisa Agropecuária (Embrapa) General Editor of 'Pesquisa Veterinária BrasileiraDepartamento de Apoio Produção e Saúde Animal Faculdade de Medicina Veterinária de Araçatuba Unesp Campus de Araçatuba, Rua Clóvis Pestana 793Programa de Pós-Graduação em Medicina Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (Unesp), Via de Acesso Professor Paulo Donato Castellane s/nDepartamento de Patologia e Propedêutica Clínica Faculdade de Odontologia de Araçatuba Unesp, Rua José Bonifácio 1193Departamento de Matemática Faculdade de Engenharia de Ilha Solteira Unesp, Alameda Rio de Janeiro 266Departamento de Apoio Produção e Saúde Animal Faculdade de Medicina Veterinária de Araçatuba Unesp Campus de Araçatuba, Rua Clóvis Pestana 793Universidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Borsanelli, Ana Carolina [UNESP]Júnior, Elerson Gaetti-Jardim [UNESP]Schweitzer, Christiane Marie [UNESP]Döbereiner, JürgenDutra, Iveraldo S. [UNESP]2018-12-11T16:59:34Z2018-12-11T16:59:34Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article829-834application/pdfhttp://dx.doi.org/10.1590/s0100-736x2015001000002Pesquisa Veterinaria Brasileira, v. 35, n. 10, p. 829-834, 2015.1678-51500100-736Xhttp://hdl.handle.net/11449/17229310.1590/s0100-736x2015001000002S0100-736X20150010008292-s2.0-84949745362S0100-736X2015001000829.pdf00011553552103270000-0001-9561-8281Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPesquisa Veterinaria Brasileirainfo:eu-repo/semantics/openAccess2024-01-06T06:27:53Zoai:repositorio.unesp.br:11449/172293Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-06T06:27:53Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Presence of Porphyromonas and Prevotella species in the oral microflora of cattle with periodontitis
title Presence of Porphyromonas and Prevotella species in the oral microflora of cattle with periodontitis
spellingShingle Presence of Porphyromonas and Prevotella species in the oral microflora of cattle with periodontitis
Borsanelli, Ana Carolina [UNESP]
Cattle
Periodontitis
Porphyromonas spp.
Prevotella spp.
title_short Presence of Porphyromonas and Prevotella species in the oral microflora of cattle with periodontitis
title_full Presence of Porphyromonas and Prevotella species in the oral microflora of cattle with periodontitis
title_fullStr Presence of Porphyromonas and Prevotella species in the oral microflora of cattle with periodontitis
title_full_unstemmed Presence of Porphyromonas and Prevotella species in the oral microflora of cattle with periodontitis
title_sort Presence of Porphyromonas and Prevotella species in the oral microflora of cattle with periodontitis
author Borsanelli, Ana Carolina [UNESP]
author_facet Borsanelli, Ana Carolina [UNESP]
Júnior, Elerson Gaetti-Jardim [UNESP]
Schweitzer, Christiane Marie [UNESP]
Döbereiner, Jürgen
Dutra, Iveraldo S. [UNESP]
author_role author
author2 Júnior, Elerson Gaetti-Jardim [UNESP]
Schweitzer, Christiane Marie [UNESP]
Döbereiner, Jürgen
Dutra, Iveraldo S. [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.author.fl_str_mv Borsanelli, Ana Carolina [UNESP]
Júnior, Elerson Gaetti-Jardim [UNESP]
Schweitzer, Christiane Marie [UNESP]
Döbereiner, Jürgen
Dutra, Iveraldo S. [UNESP]
dc.subject.por.fl_str_mv Cattle
Periodontitis
Porphyromonas spp.
Prevotella spp.
topic Cattle
Periodontitis
Porphyromonas spp.
Prevotella spp.
description Bovine periodontitis is a progressive purulent infectious process associated with the presence of strictly and facultative anaerobic subgingival biofilm and epidemiologically related to soil management in large geographic areas of Brazil. This study aimed to detect species of the genera Porphyromonas and Prevotella, which occurr in periodontal pockets of cattle with lesions deeper than 5mm (n=26) and in gingival sulcus of animals considered periodontally healthy (n=25). Presence of the microorganisms was evaluated by independent- culture medium diagnostic method, using polymerase chain reaction (PCR) with specific primers of Porphyromonas asaccharolytica, P. endodontalis, P. gingivalis, P. gulae, Prevotella buccae, P. intermedia, P. loescheii, P. melaninogenica, P. nigrescens, P. oralis and P. tannerae. The species P. endodontalis (80.7%), P. melaninogenica (73.1%) and P. intermedia (61.5%) were the most predominant in samples of cattle with periodontitis. Regarding non -injured gingival sulcus of cattle, P. endodontalis (40%) and P. loeschei (40%) prevailed. Porphyromonas gingivalis, P. gulae and Prevotella tannerae were not detected in the 51 samples studied. Data evaluation by T test, enabled to verify that ocorrence of Porphyromonas asaccharolytica (p=0.000003), P. endodontalis (p=0.0023), Prevotella buccae (p=0.0017), P. intermedia (p=0.0020), P. melaninogenica (p=0.00006) and P. oralis (p=0.0028) is correlated with bovine periodontitis.
publishDate 2015
dc.date.none.fl_str_mv 2015-01-01
2018-12-11T16:59:34Z
2018-12-11T16:59:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/s0100-736x2015001000002
Pesquisa Veterinaria Brasileira, v. 35, n. 10, p. 829-834, 2015.
1678-5150
0100-736X
http://hdl.handle.net/11449/172293
10.1590/s0100-736x2015001000002
S0100-736X2015001000829
2-s2.0-84949745362
S0100-736X2015001000829.pdf
0001155355210327
0000-0001-9561-8281
url http://dx.doi.org/10.1590/s0100-736x2015001000002
http://hdl.handle.net/11449/172293
identifier_str_mv Pesquisa Veterinaria Brasileira, v. 35, n. 10, p. 829-834, 2015.
1678-5150
0100-736X
10.1590/s0100-736x2015001000002
S0100-736X2015001000829
2-s2.0-84949745362
S0100-736X2015001000829.pdf
0001155355210327
0000-0001-9561-8281
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pesquisa Veterinaria Brasileira
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 829-834
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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