First molecular detection of Mycoplasma ovis in horses from Brazil
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.actatropica.2022.106697 http://hdl.handle.net/11449/247729 |
Resumo: | This study aimed to determine the occurrence of hemoplasmas and tick-borne pathogens (TBP) (Theileria equi, Babesia caballi, and Ehrlichia sp.) in horses and ticks’ salivary glands, and determine the factors associated with exposure/infection in a rural settlement in southern Brazil. Blood samples from 22 horses were screened for anti-T. equi and anti-Ehrlichia sp. antibodies by an indirect fluorescent antibody test (IFAT) assays. Samples were also tested by PCR assays for T. equi and B. caballi (18S rRNA and rap-1 genes, respectively), hemoplasmas (16S rRNA gene), and Ehrlichia sp. (dsb gene). Ticks were removed from the animals (inspection) and the environment (flannel trawling and dry ice traps), and morphologically identified. Additionally, salivary glands DNA was extracted from 28 adult ticks infesting the animals and four nymphs from the environment, and further screened for Ehrlichia sp. and hemotropic Mycoplasma sp. Anti-T. equi and anti-Ehrlichia sp. antibodies were detected in 40.91% (nine/22; 95% CI: 23.26-61.27) and 31.81% (seven/22; 95% CI: 16.36-52.68) horses, respectively. Theileria equi, B. caballi, and hemotropic Mycoplasma sp. DNA was detected in 59.09% (13/22), 4.55% (one/22), and 50% (11/22) horses, respectively. All horses tested negative in the PCR for Ehrlichia sp. All sequences showed ≥99% identity with multiple T. equi, B. caballi, and Mycoplasma ovis sequences deposited in GenBank database. Adult ticks were identified as Dermacentor nitens (44/47; 93.62%) and Rhipicephalus microplus (three/47; 6.38%). Ticks’ salivary glands were negative for Ehrlichia sp., while 39.29% from adults (11/28) and 50% from nymphs (two/four) from the environment were positive for hemotropic Mycoplasma sp. This is the first report of M. ovis infection in horses from Brazil and the first detection of hemoplasma DNA in salivary glands of D. nitens and R. microplus ticks. Further studies are needed to elucidate the vector competence of ticks to transmit hemoplasmas. |
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First molecular detection of Mycoplasma ovis in horses from BrazilBabesia caballiDermacentor nitensEhrlichia spHemotropic mycoplasmaTheileria equiThis study aimed to determine the occurrence of hemoplasmas and tick-borne pathogens (TBP) (Theileria equi, Babesia caballi, and Ehrlichia sp.) in horses and ticks’ salivary glands, and determine the factors associated with exposure/infection in a rural settlement in southern Brazil. Blood samples from 22 horses were screened for anti-T. equi and anti-Ehrlichia sp. antibodies by an indirect fluorescent antibody test (IFAT) assays. Samples were also tested by PCR assays for T. equi and B. caballi (18S rRNA and rap-1 genes, respectively), hemoplasmas (16S rRNA gene), and Ehrlichia sp. (dsb gene). Ticks were removed from the animals (inspection) and the environment (flannel trawling and dry ice traps), and morphologically identified. Additionally, salivary glands DNA was extracted from 28 adult ticks infesting the animals and four nymphs from the environment, and further screened for Ehrlichia sp. and hemotropic Mycoplasma sp. Anti-T. equi and anti-Ehrlichia sp. antibodies were detected in 40.91% (nine/22; 95% CI: 23.26-61.27) and 31.81% (seven/22; 95% CI: 16.36-52.68) horses, respectively. Theileria equi, B. caballi, and hemotropic Mycoplasma sp. DNA was detected in 59.09% (13/22), 4.55% (one/22), and 50% (11/22) horses, respectively. All horses tested negative in the PCR for Ehrlichia sp. All sequences showed ≥99% identity with multiple T. equi, B. caballi, and Mycoplasma ovis sequences deposited in GenBank database. Adult ticks were identified as Dermacentor nitens (44/47; 93.62%) and Rhipicephalus microplus (three/47; 6.38%). Ticks’ salivary glands were negative for Ehrlichia sp., while 39.29% from adults (11/28) and 50% from nymphs (two/four) from the environment were positive for hemotropic Mycoplasma sp. This is the first report of M. ovis infection in horses from Brazil and the first detection of hemoplasma DNA in salivary glands of D. nitens and R. microplus ticks. Further studies are needed to elucidate the vector competence of ticks to transmit hemoplasmas.Vector-Borne Diseases Laboratory Department of Veterinary Medicine Universidade Federal do Paraná Rua dos Funcionários, Campus Agrárias, 1540, Juvevê, PRDepartment of Preventive Veterinary Medicine Universidade Estadual de LondrinaLaboratory of Immunoparasitology Department of Immunology Microbiology and Parasitology Universidade Federal de Uberlândia, UberlândiaLaboratório de Imunoparasitologia Departamento de Patologia Teriogenologia e Saúde Única Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (FCAV/UNESP)Laboratório de Virologia e Rickettsioses Universidade Federal do Mato Grosso, CuiabáGlobal One Health initiative (GOHi) The Ohio State UniversityLaboratório de Imunoparasitologia Departamento de Patologia Teriogenologia e Saúde Única Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (FCAV/UNESP)Universidade Federal do Paraná (UFPR)Universidade Estadual de Londrina (UEL)Universidade Federal de Uberlândia (UFU)Universidade Estadual Paulista (UNESP)Universidade Federal do Mato GrossoThe Ohio State UniversityKakimori, Monica T.A.Barros, Luiz D.Collere, Flávia C.M.Ferrari, Larissa D.R.de Matos, AldairLucas, Juliana I.Coradi, Vanessa S.Mongruel, Anna C.B. [UNESP]Aguiar, Daniel M.Machado, Rosangela Z. [UNESP]André, Marcos R. [UNESP]Vieira, Thállitha S.W.J.Vieira, Rafael F.C.2023-07-29T13:24:16Z2023-07-29T13:24:16Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.actatropica.2022.106697Acta Tropica, v. 237.1873-62540001-706Xhttp://hdl.handle.net/11449/24772910.1016/j.actatropica.2022.1066972-s2.0-85139624295Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Tropicainfo:eu-repo/semantics/openAccess2024-06-07T13:02:37Zoai:repositorio.unesp.br:11449/247729Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:39:24.816253Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
First molecular detection of Mycoplasma ovis in horses from Brazil |
title |
First molecular detection of Mycoplasma ovis in horses from Brazil |
spellingShingle |
First molecular detection of Mycoplasma ovis in horses from Brazil Kakimori, Monica T.A. Babesia caballi Dermacentor nitens Ehrlichia sp Hemotropic mycoplasma Theileria equi |
title_short |
First molecular detection of Mycoplasma ovis in horses from Brazil |
title_full |
First molecular detection of Mycoplasma ovis in horses from Brazil |
title_fullStr |
First molecular detection of Mycoplasma ovis in horses from Brazil |
title_full_unstemmed |
First molecular detection of Mycoplasma ovis in horses from Brazil |
title_sort |
First molecular detection of Mycoplasma ovis in horses from Brazil |
author |
Kakimori, Monica T.A. |
author_facet |
Kakimori, Monica T.A. Barros, Luiz D. Collere, Flávia C.M. Ferrari, Larissa D.R. de Matos, Aldair Lucas, Juliana I. Coradi, Vanessa S. Mongruel, Anna C.B. [UNESP] Aguiar, Daniel M. Machado, Rosangela Z. [UNESP] André, Marcos R. [UNESP] Vieira, Thállitha S.W.J. Vieira, Rafael F.C. |
author_role |
author |
author2 |
Barros, Luiz D. Collere, Flávia C.M. Ferrari, Larissa D.R. de Matos, Aldair Lucas, Juliana I. Coradi, Vanessa S. Mongruel, Anna C.B. [UNESP] Aguiar, Daniel M. Machado, Rosangela Z. [UNESP] André, Marcos R. [UNESP] Vieira, Thállitha S.W.J. Vieira, Rafael F.C. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Paraná (UFPR) Universidade Estadual de Londrina (UEL) Universidade Federal de Uberlândia (UFU) Universidade Estadual Paulista (UNESP) Universidade Federal do Mato Grosso The Ohio State University |
dc.contributor.author.fl_str_mv |
Kakimori, Monica T.A. Barros, Luiz D. Collere, Flávia C.M. Ferrari, Larissa D.R. de Matos, Aldair Lucas, Juliana I. Coradi, Vanessa S. Mongruel, Anna C.B. [UNESP] Aguiar, Daniel M. Machado, Rosangela Z. [UNESP] André, Marcos R. [UNESP] Vieira, Thállitha S.W.J. Vieira, Rafael F.C. |
dc.subject.por.fl_str_mv |
Babesia caballi Dermacentor nitens Ehrlichia sp Hemotropic mycoplasma Theileria equi |
topic |
Babesia caballi Dermacentor nitens Ehrlichia sp Hemotropic mycoplasma Theileria equi |
description |
This study aimed to determine the occurrence of hemoplasmas and tick-borne pathogens (TBP) (Theileria equi, Babesia caballi, and Ehrlichia sp.) in horses and ticks’ salivary glands, and determine the factors associated with exposure/infection in a rural settlement in southern Brazil. Blood samples from 22 horses were screened for anti-T. equi and anti-Ehrlichia sp. antibodies by an indirect fluorescent antibody test (IFAT) assays. Samples were also tested by PCR assays for T. equi and B. caballi (18S rRNA and rap-1 genes, respectively), hemoplasmas (16S rRNA gene), and Ehrlichia sp. (dsb gene). Ticks were removed from the animals (inspection) and the environment (flannel trawling and dry ice traps), and morphologically identified. Additionally, salivary glands DNA was extracted from 28 adult ticks infesting the animals and four nymphs from the environment, and further screened for Ehrlichia sp. and hemotropic Mycoplasma sp. Anti-T. equi and anti-Ehrlichia sp. antibodies were detected in 40.91% (nine/22; 95% CI: 23.26-61.27) and 31.81% (seven/22; 95% CI: 16.36-52.68) horses, respectively. Theileria equi, B. caballi, and hemotropic Mycoplasma sp. DNA was detected in 59.09% (13/22), 4.55% (one/22), and 50% (11/22) horses, respectively. All horses tested negative in the PCR for Ehrlichia sp. All sequences showed ≥99% identity with multiple T. equi, B. caballi, and Mycoplasma ovis sequences deposited in GenBank database. Adult ticks were identified as Dermacentor nitens (44/47; 93.62%) and Rhipicephalus microplus (three/47; 6.38%). Ticks’ salivary glands were negative for Ehrlichia sp., while 39.29% from adults (11/28) and 50% from nymphs (two/four) from the environment were positive for hemotropic Mycoplasma sp. This is the first report of M. ovis infection in horses from Brazil and the first detection of hemoplasma DNA in salivary glands of D. nitens and R. microplus ticks. Further studies are needed to elucidate the vector competence of ticks to transmit hemoplasmas. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:24:16Z 2023-07-29T13:24:16Z 2023-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.actatropica.2022.106697 Acta Tropica, v. 237. 1873-6254 0001-706X http://hdl.handle.net/11449/247729 10.1016/j.actatropica.2022.106697 2-s2.0-85139624295 |
url |
http://dx.doi.org/10.1016/j.actatropica.2022.106697 http://hdl.handle.net/11449/247729 |
identifier_str_mv |
Acta Tropica, v. 237. 1873-6254 0001-706X 10.1016/j.actatropica.2022.106697 2-s2.0-85139624295 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Tropica |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129101129580544 |