Application of methylotrophic yeast Pichia pastoris in the field of food industry - A review
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/225485 |
Resumo: | Since ancient times, the utilization of yeasts by the man has a great impact on the socio-economic development. After the advent of the technology of recombinant DNA, great advances have occurred due to the acquisition of strains of mutant yeasts in the field of applied research, and Saccharomyces cerevisiae has soon been outstanding as an interesting candidate for the expression of heterologous proteins of biotechnological interest. As the time goes by other alternative systems of expression have been shown because they have advantages over Saccharomyces cerevisiae. Among those new systems, Pichia pastoris is outstanding as methylotrophic yeast capable of growing in a culture medium containing methanol as the only source of carbon and energy. The induction of production of glycerol-3-phosphate dehydrogenase (GPD, NAD+: oxido-redutase EC 1.1.1.8) by Pichia pastoris was accomplished in the medium containing methanol. One of the most important key parameters in Pichia pastoris expression system is the methanol concentration. Bibliographic reviews on the Pichia pastoris production system have shown that the best culture conditions vary according to the strain used and/or kind of heterologous protein desired to be expressed. Therefore, we have sought to develop a system, involving expression of glycerol-3-phosphate dehydrogenase in the yeast Pichia pastoris, for generating sufficient quantities of the enzyme in order to asses its potential value for use in various food bioanalytical determination. Dehydrogenases have been widely used in the enzymatic assays of diverse composites of industrial interest, being enclosed among them glycerol and a number of important bioanalytical applications. |
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Application of methylotrophic yeast Pichia pastoris in the field of food industry - A reviewCulture mediumExpressionGlycerol-3-phosphate dehydrogenaseOptimizationPichia pastorisYeastSince ancient times, the utilization of yeasts by the man has a great impact on the socio-economic development. After the advent of the technology of recombinant DNA, great advances have occurred due to the acquisition of strains of mutant yeasts in the field of applied research, and Saccharomyces cerevisiae has soon been outstanding as an interesting candidate for the expression of heterologous proteins of biotechnological interest. As the time goes by other alternative systems of expression have been shown because they have advantages over Saccharomyces cerevisiae. Among those new systems, Pichia pastoris is outstanding as methylotrophic yeast capable of growing in a culture medium containing methanol as the only source of carbon and energy. The induction of production of glycerol-3-phosphate dehydrogenase (GPD, NAD+: oxido-redutase EC 1.1.1.8) by Pichia pastoris was accomplished in the medium containing methanol. One of the most important key parameters in Pichia pastoris expression system is the methanol concentration. Bibliographic reviews on the Pichia pastoris production system have shown that the best culture conditions vary according to the strain used and/or kind of heterologous protein desired to be expressed. Therefore, we have sought to develop a system, involving expression of glycerol-3-phosphate dehydrogenase in the yeast Pichia pastoris, for generating sufficient quantities of the enzyme in order to asses its potential value for use in various food bioanalytical determination. Dehydrogenases have been widely used in the enzymatic assays of diverse composites of industrial interest, being enclosed among them glycerol and a number of important bioanalytical applications.Departament of Food and Nutrition Faculdade de Ciências Farmacêuticas UNESP - São Paulo State University, Rodovia Araraquara-Jaú, Km 1, 14801-902, São PauloDepartament of Food and Nutrition Faculdade de Ciências Farmacêuticas UNESP - São Paulo State University, Rodovia Araraquara-Jaú, Km 1, 14801-902, São PauloUniversidade Estadual Paulista (UNESP)Silva, Viviane C. [UNESP]Peres, Maristela F. S. [UNESP]Gattás, Edwil A. L. [UNESP]2022-04-28T20:52:15Z2022-04-28T20:52:15Z2009-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article268-273Journal of Food, Agriculture and Environment, v. 7, n. 2, p. 268-273, 2009.1459-02631459-0255http://hdl.handle.net/11449/2254852-s2.0-66349118614Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Food, Agriculture and Environmentinfo:eu-repo/semantics/openAccess2024-06-21T12:47:01Zoai:repositorio.unesp.br:11449/225485Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:39:03.721747Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Application of methylotrophic yeast Pichia pastoris in the field of food industry - A review |
title |
Application of methylotrophic yeast Pichia pastoris in the field of food industry - A review |
spellingShingle |
Application of methylotrophic yeast Pichia pastoris in the field of food industry - A review Silva, Viviane C. [UNESP] Culture medium Expression Glycerol-3-phosphate dehydrogenase Optimization Pichia pastoris Yeast |
title_short |
Application of methylotrophic yeast Pichia pastoris in the field of food industry - A review |
title_full |
Application of methylotrophic yeast Pichia pastoris in the field of food industry - A review |
title_fullStr |
Application of methylotrophic yeast Pichia pastoris in the field of food industry - A review |
title_full_unstemmed |
Application of methylotrophic yeast Pichia pastoris in the field of food industry - A review |
title_sort |
Application of methylotrophic yeast Pichia pastoris in the field of food industry - A review |
author |
Silva, Viviane C. [UNESP] |
author_facet |
Silva, Viviane C. [UNESP] Peres, Maristela F. S. [UNESP] Gattás, Edwil A. L. [UNESP] |
author_role |
author |
author2 |
Peres, Maristela F. S. [UNESP] Gattás, Edwil A. L. [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Silva, Viviane C. [UNESP] Peres, Maristela F. S. [UNESP] Gattás, Edwil A. L. [UNESP] |
dc.subject.por.fl_str_mv |
Culture medium Expression Glycerol-3-phosphate dehydrogenase Optimization Pichia pastoris Yeast |
topic |
Culture medium Expression Glycerol-3-phosphate dehydrogenase Optimization Pichia pastoris Yeast |
description |
Since ancient times, the utilization of yeasts by the man has a great impact on the socio-economic development. After the advent of the technology of recombinant DNA, great advances have occurred due to the acquisition of strains of mutant yeasts in the field of applied research, and Saccharomyces cerevisiae has soon been outstanding as an interesting candidate for the expression of heterologous proteins of biotechnological interest. As the time goes by other alternative systems of expression have been shown because they have advantages over Saccharomyces cerevisiae. Among those new systems, Pichia pastoris is outstanding as methylotrophic yeast capable of growing in a culture medium containing methanol as the only source of carbon and energy. The induction of production of glycerol-3-phosphate dehydrogenase (GPD, NAD+: oxido-redutase EC 1.1.1.8) by Pichia pastoris was accomplished in the medium containing methanol. One of the most important key parameters in Pichia pastoris expression system is the methanol concentration. Bibliographic reviews on the Pichia pastoris production system have shown that the best culture conditions vary according to the strain used and/or kind of heterologous protein desired to be expressed. Therefore, we have sought to develop a system, involving expression of glycerol-3-phosphate dehydrogenase in the yeast Pichia pastoris, for generating sufficient quantities of the enzyme in order to asses its potential value for use in various food bioanalytical determination. Dehydrogenases have been widely used in the enzymatic assays of diverse composites of industrial interest, being enclosed among them glycerol and a number of important bioanalytical applications. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-01-01 2022-04-28T20:52:15Z 2022-04-28T20:52:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Journal of Food, Agriculture and Environment, v. 7, n. 2, p. 268-273, 2009. 1459-0263 1459-0255 http://hdl.handle.net/11449/225485 2-s2.0-66349118614 |
identifier_str_mv |
Journal of Food, Agriculture and Environment, v. 7, n. 2, p. 268-273, 2009. 1459-0263 1459-0255 2-s2.0-66349118614 |
url |
http://hdl.handle.net/11449/225485 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Food, Agriculture and Environment |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
268-273 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129100362022912 |