Application of methylotrophic yeast Pichia pastoris in the field of food industry - A review

Detalhes bibliográficos
Autor(a) principal: Silva, Viviane C. [UNESP]
Data de Publicação: 2009
Outros Autores: Peres, Maristela F. S. [UNESP], Gattás, Edwil A. L. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/225485
Resumo: Since ancient times, the utilization of yeasts by the man has a great impact on the socio-economic development. After the advent of the technology of recombinant DNA, great advances have occurred due to the acquisition of strains of mutant yeasts in the field of applied research, and Saccharomyces cerevisiae has soon been outstanding as an interesting candidate for the expression of heterologous proteins of biotechnological interest. As the time goes by other alternative systems of expression have been shown because they have advantages over Saccharomyces cerevisiae. Among those new systems, Pichia pastoris is outstanding as methylotrophic yeast capable of growing in a culture medium containing methanol as the only source of carbon and energy. The induction of production of glycerol-3-phosphate dehydrogenase (GPD, NAD+: oxido-redutase EC 1.1.1.8) by Pichia pastoris was accomplished in the medium containing methanol. One of the most important key parameters in Pichia pastoris expression system is the methanol concentration. Bibliographic reviews on the Pichia pastoris production system have shown that the best culture conditions vary according to the strain used and/or kind of heterologous protein desired to be expressed. Therefore, we have sought to develop a system, involving expression of glycerol-3-phosphate dehydrogenase in the yeast Pichia pastoris, for generating sufficient quantities of the enzyme in order to asses its potential value for use in various food bioanalytical determination. Dehydrogenases have been widely used in the enzymatic assays of diverse composites of industrial interest, being enclosed among them glycerol and a number of important bioanalytical applications.
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spelling Application of methylotrophic yeast Pichia pastoris in the field of food industry - A reviewCulture mediumExpressionGlycerol-3-phosphate dehydrogenaseOptimizationPichia pastorisYeastSince ancient times, the utilization of yeasts by the man has a great impact on the socio-economic development. After the advent of the technology of recombinant DNA, great advances have occurred due to the acquisition of strains of mutant yeasts in the field of applied research, and Saccharomyces cerevisiae has soon been outstanding as an interesting candidate for the expression of heterologous proteins of biotechnological interest. As the time goes by other alternative systems of expression have been shown because they have advantages over Saccharomyces cerevisiae. Among those new systems, Pichia pastoris is outstanding as methylotrophic yeast capable of growing in a culture medium containing methanol as the only source of carbon and energy. The induction of production of glycerol-3-phosphate dehydrogenase (GPD, NAD+: oxido-redutase EC 1.1.1.8) by Pichia pastoris was accomplished in the medium containing methanol. One of the most important key parameters in Pichia pastoris expression system is the methanol concentration. Bibliographic reviews on the Pichia pastoris production system have shown that the best culture conditions vary according to the strain used and/or kind of heterologous protein desired to be expressed. Therefore, we have sought to develop a system, involving expression of glycerol-3-phosphate dehydrogenase in the yeast Pichia pastoris, for generating sufficient quantities of the enzyme in order to asses its potential value for use in various food bioanalytical determination. Dehydrogenases have been widely used in the enzymatic assays of diverse composites of industrial interest, being enclosed among them glycerol and a number of important bioanalytical applications.Departament of Food and Nutrition Faculdade de Ciências Farmacêuticas UNESP - São Paulo State University, Rodovia Araraquara-Jaú, Km 1, 14801-902, São PauloDepartament of Food and Nutrition Faculdade de Ciências Farmacêuticas UNESP - São Paulo State University, Rodovia Araraquara-Jaú, Km 1, 14801-902, São PauloUniversidade Estadual Paulista (UNESP)Silva, Viviane C. [UNESP]Peres, Maristela F. S. [UNESP]Gattás, Edwil A. L. [UNESP]2022-04-28T20:52:15Z2022-04-28T20:52:15Z2009-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article268-273Journal of Food, Agriculture and Environment, v. 7, n. 2, p. 268-273, 2009.1459-02631459-0255http://hdl.handle.net/11449/2254852-s2.0-66349118614Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Food, Agriculture and Environmentinfo:eu-repo/semantics/openAccess2024-06-21T12:47:01Zoai:repositorio.unesp.br:11449/225485Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:39:03.721747Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Application of methylotrophic yeast Pichia pastoris in the field of food industry - A review
title Application of methylotrophic yeast Pichia pastoris in the field of food industry - A review
spellingShingle Application of methylotrophic yeast Pichia pastoris in the field of food industry - A review
Silva, Viviane C. [UNESP]
Culture medium
Expression
Glycerol-3-phosphate dehydrogenase
Optimization
Pichia pastoris
Yeast
title_short Application of methylotrophic yeast Pichia pastoris in the field of food industry - A review
title_full Application of methylotrophic yeast Pichia pastoris in the field of food industry - A review
title_fullStr Application of methylotrophic yeast Pichia pastoris in the field of food industry - A review
title_full_unstemmed Application of methylotrophic yeast Pichia pastoris in the field of food industry - A review
title_sort Application of methylotrophic yeast Pichia pastoris in the field of food industry - A review
author Silva, Viviane C. [UNESP]
author_facet Silva, Viviane C. [UNESP]
Peres, Maristela F. S. [UNESP]
Gattás, Edwil A. L. [UNESP]
author_role author
author2 Peres, Maristela F. S. [UNESP]
Gattás, Edwil A. L. [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Silva, Viviane C. [UNESP]
Peres, Maristela F. S. [UNESP]
Gattás, Edwil A. L. [UNESP]
dc.subject.por.fl_str_mv Culture medium
Expression
Glycerol-3-phosphate dehydrogenase
Optimization
Pichia pastoris
Yeast
topic Culture medium
Expression
Glycerol-3-phosphate dehydrogenase
Optimization
Pichia pastoris
Yeast
description Since ancient times, the utilization of yeasts by the man has a great impact on the socio-economic development. After the advent of the technology of recombinant DNA, great advances have occurred due to the acquisition of strains of mutant yeasts in the field of applied research, and Saccharomyces cerevisiae has soon been outstanding as an interesting candidate for the expression of heterologous proteins of biotechnological interest. As the time goes by other alternative systems of expression have been shown because they have advantages over Saccharomyces cerevisiae. Among those new systems, Pichia pastoris is outstanding as methylotrophic yeast capable of growing in a culture medium containing methanol as the only source of carbon and energy. The induction of production of glycerol-3-phosphate dehydrogenase (GPD, NAD+: oxido-redutase EC 1.1.1.8) by Pichia pastoris was accomplished in the medium containing methanol. One of the most important key parameters in Pichia pastoris expression system is the methanol concentration. Bibliographic reviews on the Pichia pastoris production system have shown that the best culture conditions vary according to the strain used and/or kind of heterologous protein desired to be expressed. Therefore, we have sought to develop a system, involving expression of glycerol-3-phosphate dehydrogenase in the yeast Pichia pastoris, for generating sufficient quantities of the enzyme in order to asses its potential value for use in various food bioanalytical determination. Dehydrogenases have been widely used in the enzymatic assays of diverse composites of industrial interest, being enclosed among them glycerol and a number of important bioanalytical applications.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-01
2022-04-28T20:52:15Z
2022-04-28T20:52:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Journal of Food, Agriculture and Environment, v. 7, n. 2, p. 268-273, 2009.
1459-0263
1459-0255
http://hdl.handle.net/11449/225485
2-s2.0-66349118614
identifier_str_mv Journal of Food, Agriculture and Environment, v. 7, n. 2, p. 268-273, 2009.
1459-0263
1459-0255
2-s2.0-66349118614
url http://hdl.handle.net/11449/225485
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Food, Agriculture and Environment
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 268-273
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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