High frequency and molecular characterization of porcine hemotrophic mycoplasmas in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.vetmic.2019.02.024 http://hdl.handle.net/11449/187390 |
Resumo: | Mycoplasma suis and Mycoplasma parvum are the two hemotrophic mycoplasmas species described in pigs. M. suis is involved in infectious anemia, while M parvum infection is commonly subclinical. The objectives of this study were twofold: (i) to investigate the prevalence of porcine hemotrophic mycoplasmas in sows from the southern region of Brazil by quantitative real-time PCR (qPCR) and (ii) to genetically characterize a subset of the samples based on the 16S rRNA gene. A total of 429 blood samples were evaluated from 53 different farm sites. Porcine hemoplasmas was detected at all the 53 tested sites and in 79.72% of the samples (342/429). Two sequences were obtained for Mycoplasma spp. The phylogenetic analysis based on the 16S rRNA gene (900 bp) showed that the Mycoplasma sequences were closely related to the M. suis cluster and that one sequence was positioned in the M. parvum cluster. In conclusion, porcine hemoplasmas have a high rate of prevalence in sows from commercial farms in the southern region of Brazil. This study demonstrated the first molecular detection and characterization of partial 16S rRNA gene of M. parvum in Brazil. |
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High frequency and molecular characterization of porcine hemotrophic mycoplasmas in BrazilHemotrophic mycoplasmasInfectious diseasesMycoplasma parvumMycoplasma suisSowsMycoplasma suis and Mycoplasma parvum are the two hemotrophic mycoplasmas species described in pigs. M. suis is involved in infectious anemia, while M parvum infection is commonly subclinical. The objectives of this study were twofold: (i) to investigate the prevalence of porcine hemotrophic mycoplasmas in sows from the southern region of Brazil by quantitative real-time PCR (qPCR) and (ii) to genetically characterize a subset of the samples based on the 16S rRNA gene. A total of 429 blood samples were evaluated from 53 different farm sites. Porcine hemoplasmas was detected at all the 53 tested sites and in 79.72% of the samples (342/429). Two sequences were obtained for Mycoplasma spp. The phylogenetic analysis based on the 16S rRNA gene (900 bp) showed that the Mycoplasma sequences were closely related to the M. suis cluster and that one sequence was positioned in the M. parvum cluster. In conclusion, porcine hemoplasmas have a high rate of prevalence in sows from commercial farms in the southern region of Brazil. This study demonstrated the first molecular detection and characterization of partial 16S rRNA gene of M. parvum in Brazil.São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, Jaboticabal, São Paulo, Via de Acesso Prof. Paulo Donato Castellane s/nEmbrapa Swine and Poultry Animal Health Laboratory, BR 153, Km 110, P.O. Box 21, Distrito de TamanduáSão Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, Jaboticabal, São Paulo, Via de Acesso Prof. Paulo Donato Castellane s/nUniversidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Gatto, Igor Renan Honorato [UNESP]Sonálio, Karina [UNESP]Amaral, Renan Bressianini do [UNESP]Morés, NelsonDalla Costa, Osmar AntonioAndré, Marcos Rogério [UNESP]de Oliveira, Luís Guilherme [UNESP]2019-10-06T15:34:43Z2019-10-06T15:34:43Z2019-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article33-39http://dx.doi.org/10.1016/j.vetmic.2019.02.024Veterinary Microbiology, v. 231, p. 33-39.1873-25420378-1135http://hdl.handle.net/11449/18739010.1016/j.vetmic.2019.02.0242-s2.0-850619783529139899895580513Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Microbiologyinfo:eu-repo/semantics/openAccess2021-10-23T16:30:49Zoai:repositorio.unesp.br:11449/187390Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:49:25.223818Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
High frequency and molecular characterization of porcine hemotrophic mycoplasmas in Brazil |
title |
High frequency and molecular characterization of porcine hemotrophic mycoplasmas in Brazil |
spellingShingle |
High frequency and molecular characterization of porcine hemotrophic mycoplasmas in Brazil Gatto, Igor Renan Honorato [UNESP] Hemotrophic mycoplasmas Infectious diseases Mycoplasma parvum Mycoplasma suis Sows |
title_short |
High frequency and molecular characterization of porcine hemotrophic mycoplasmas in Brazil |
title_full |
High frequency and molecular characterization of porcine hemotrophic mycoplasmas in Brazil |
title_fullStr |
High frequency and molecular characterization of porcine hemotrophic mycoplasmas in Brazil |
title_full_unstemmed |
High frequency and molecular characterization of porcine hemotrophic mycoplasmas in Brazil |
title_sort |
High frequency and molecular characterization of porcine hemotrophic mycoplasmas in Brazil |
author |
Gatto, Igor Renan Honorato [UNESP] |
author_facet |
Gatto, Igor Renan Honorato [UNESP] Sonálio, Karina [UNESP] Amaral, Renan Bressianini do [UNESP] Morés, Nelson Dalla Costa, Osmar Antonio André, Marcos Rogério [UNESP] de Oliveira, Luís Guilherme [UNESP] |
author_role |
author |
author2 |
Sonálio, Karina [UNESP] Amaral, Renan Bressianini do [UNESP] Morés, Nelson Dalla Costa, Osmar Antonio André, Marcos Rogério [UNESP] de Oliveira, Luís Guilherme [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Gatto, Igor Renan Honorato [UNESP] Sonálio, Karina [UNESP] Amaral, Renan Bressianini do [UNESP] Morés, Nelson Dalla Costa, Osmar Antonio André, Marcos Rogério [UNESP] de Oliveira, Luís Guilherme [UNESP] |
dc.subject.por.fl_str_mv |
Hemotrophic mycoplasmas Infectious diseases Mycoplasma parvum Mycoplasma suis Sows |
topic |
Hemotrophic mycoplasmas Infectious diseases Mycoplasma parvum Mycoplasma suis Sows |
description |
Mycoplasma suis and Mycoplasma parvum are the two hemotrophic mycoplasmas species described in pigs. M. suis is involved in infectious anemia, while M parvum infection is commonly subclinical. The objectives of this study were twofold: (i) to investigate the prevalence of porcine hemotrophic mycoplasmas in sows from the southern region of Brazil by quantitative real-time PCR (qPCR) and (ii) to genetically characterize a subset of the samples based on the 16S rRNA gene. A total of 429 blood samples were evaluated from 53 different farm sites. Porcine hemoplasmas was detected at all the 53 tested sites and in 79.72% of the samples (342/429). Two sequences were obtained for Mycoplasma spp. The phylogenetic analysis based on the 16S rRNA gene (900 bp) showed that the Mycoplasma sequences were closely related to the M. suis cluster and that one sequence was positioned in the M. parvum cluster. In conclusion, porcine hemoplasmas have a high rate of prevalence in sows from commercial farms in the southern region of Brazil. This study demonstrated the first molecular detection and characterization of partial 16S rRNA gene of M. parvum in Brazil. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T15:34:43Z 2019-10-06T15:34:43Z 2019-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.vetmic.2019.02.024 Veterinary Microbiology, v. 231, p. 33-39. 1873-2542 0378-1135 http://hdl.handle.net/11449/187390 10.1016/j.vetmic.2019.02.024 2-s2.0-85061978352 9139899895580513 |
url |
http://dx.doi.org/10.1016/j.vetmic.2019.02.024 http://hdl.handle.net/11449/187390 |
identifier_str_mv |
Veterinary Microbiology, v. 231, p. 33-39. 1873-2542 0378-1135 10.1016/j.vetmic.2019.02.024 2-s2.0-85061978352 9139899895580513 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Veterinary Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
33-39 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128279473815552 |