High frequency and molecular characterization of porcine hemotrophic mycoplasmas in Brazil

Detalhes bibliográficos
Autor(a) principal: Gatto, Igor Renan Honorato [UNESP]
Data de Publicação: 2019
Outros Autores: Sonálio, Karina [UNESP], Amaral, Renan Bressianini do [UNESP], Morés, Nelson, Dalla Costa, Osmar Antonio, André, Marcos Rogério [UNESP], de Oliveira, Luís Guilherme [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.vetmic.2019.02.024
http://hdl.handle.net/11449/187390
Resumo: Mycoplasma suis and Mycoplasma parvum are the two hemotrophic mycoplasmas species described in pigs. M. suis is involved in infectious anemia, while M parvum infection is commonly subclinical. The objectives of this study were twofold: (i) to investigate the prevalence of porcine hemotrophic mycoplasmas in sows from the southern region of Brazil by quantitative real-time PCR (qPCR) and (ii) to genetically characterize a subset of the samples based on the 16S rRNA gene. A total of 429 blood samples were evaluated from 53 different farm sites. Porcine hemoplasmas was detected at all the 53 tested sites and in 79.72% of the samples (342/429). Two sequences were obtained for Mycoplasma spp. The phylogenetic analysis based on the 16S rRNA gene (900 bp) showed that the Mycoplasma sequences were closely related to the M. suis cluster and that one sequence was positioned in the M. parvum cluster. In conclusion, porcine hemoplasmas have a high rate of prevalence in sows from commercial farms in the southern region of Brazil. This study demonstrated the first molecular detection and characterization of partial 16S rRNA gene of M. parvum in Brazil.
id UNSP_5c1fe1b7aa50493e3f71126e5a0a6e15
oai_identifier_str oai:repositorio.unesp.br:11449/187390
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling High frequency and molecular characterization of porcine hemotrophic mycoplasmas in BrazilHemotrophic mycoplasmasInfectious diseasesMycoplasma parvumMycoplasma suisSowsMycoplasma suis and Mycoplasma parvum are the two hemotrophic mycoplasmas species described in pigs. M. suis is involved in infectious anemia, while M parvum infection is commonly subclinical. The objectives of this study were twofold: (i) to investigate the prevalence of porcine hemotrophic mycoplasmas in sows from the southern region of Brazil by quantitative real-time PCR (qPCR) and (ii) to genetically characterize a subset of the samples based on the 16S rRNA gene. A total of 429 blood samples were evaluated from 53 different farm sites. Porcine hemoplasmas was detected at all the 53 tested sites and in 79.72% of the samples (342/429). Two sequences were obtained for Mycoplasma spp. The phylogenetic analysis based on the 16S rRNA gene (900 bp) showed that the Mycoplasma sequences were closely related to the M. suis cluster and that one sequence was positioned in the M. parvum cluster. In conclusion, porcine hemoplasmas have a high rate of prevalence in sows from commercial farms in the southern region of Brazil. This study demonstrated the first molecular detection and characterization of partial 16S rRNA gene of M. parvum in Brazil.São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, Jaboticabal, São Paulo, Via de Acesso Prof. Paulo Donato Castellane s/nEmbrapa Swine and Poultry Animal Health Laboratory, BR 153, Km 110, P.O. Box 21, Distrito de TamanduáSão Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, Jaboticabal, São Paulo, Via de Acesso Prof. Paulo Donato Castellane s/nUniversidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Gatto, Igor Renan Honorato [UNESP]Sonálio, Karina [UNESP]Amaral, Renan Bressianini do [UNESP]Morés, NelsonDalla Costa, Osmar AntonioAndré, Marcos Rogério [UNESP]de Oliveira, Luís Guilherme [UNESP]2019-10-06T15:34:43Z2019-10-06T15:34:43Z2019-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article33-39http://dx.doi.org/10.1016/j.vetmic.2019.02.024Veterinary Microbiology, v. 231, p. 33-39.1873-25420378-1135http://hdl.handle.net/11449/18739010.1016/j.vetmic.2019.02.0242-s2.0-850619783529139899895580513Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Microbiologyinfo:eu-repo/semantics/openAccess2021-10-23T16:30:49Zoai:repositorio.unesp.br:11449/187390Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:49:25.223818Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv High frequency and molecular characterization of porcine hemotrophic mycoplasmas in Brazil
title High frequency and molecular characterization of porcine hemotrophic mycoplasmas in Brazil
spellingShingle High frequency and molecular characterization of porcine hemotrophic mycoplasmas in Brazil
Gatto, Igor Renan Honorato [UNESP]
Hemotrophic mycoplasmas
Infectious diseases
Mycoplasma parvum
Mycoplasma suis
Sows
title_short High frequency and molecular characterization of porcine hemotrophic mycoplasmas in Brazil
title_full High frequency and molecular characterization of porcine hemotrophic mycoplasmas in Brazil
title_fullStr High frequency and molecular characterization of porcine hemotrophic mycoplasmas in Brazil
title_full_unstemmed High frequency and molecular characterization of porcine hemotrophic mycoplasmas in Brazil
title_sort High frequency and molecular characterization of porcine hemotrophic mycoplasmas in Brazil
author Gatto, Igor Renan Honorato [UNESP]
author_facet Gatto, Igor Renan Honorato [UNESP]
Sonálio, Karina [UNESP]
Amaral, Renan Bressianini do [UNESP]
Morés, Nelson
Dalla Costa, Osmar Antonio
André, Marcos Rogério [UNESP]
de Oliveira, Luís Guilherme [UNESP]
author_role author
author2 Sonálio, Karina [UNESP]
Amaral, Renan Bressianini do [UNESP]
Morés, Nelson
Dalla Costa, Osmar Antonio
André, Marcos Rogério [UNESP]
de Oliveira, Luís Guilherme [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.author.fl_str_mv Gatto, Igor Renan Honorato [UNESP]
Sonálio, Karina [UNESP]
Amaral, Renan Bressianini do [UNESP]
Morés, Nelson
Dalla Costa, Osmar Antonio
André, Marcos Rogério [UNESP]
de Oliveira, Luís Guilherme [UNESP]
dc.subject.por.fl_str_mv Hemotrophic mycoplasmas
Infectious diseases
Mycoplasma parvum
Mycoplasma suis
Sows
topic Hemotrophic mycoplasmas
Infectious diseases
Mycoplasma parvum
Mycoplasma suis
Sows
description Mycoplasma suis and Mycoplasma parvum are the two hemotrophic mycoplasmas species described in pigs. M. suis is involved in infectious anemia, while M parvum infection is commonly subclinical. The objectives of this study were twofold: (i) to investigate the prevalence of porcine hemotrophic mycoplasmas in sows from the southern region of Brazil by quantitative real-time PCR (qPCR) and (ii) to genetically characterize a subset of the samples based on the 16S rRNA gene. A total of 429 blood samples were evaluated from 53 different farm sites. Porcine hemoplasmas was detected at all the 53 tested sites and in 79.72% of the samples (342/429). Two sequences were obtained for Mycoplasma spp. The phylogenetic analysis based on the 16S rRNA gene (900 bp) showed that the Mycoplasma sequences were closely related to the M. suis cluster and that one sequence was positioned in the M. parvum cluster. In conclusion, porcine hemoplasmas have a high rate of prevalence in sows from commercial farms in the southern region of Brazil. This study demonstrated the first molecular detection and characterization of partial 16S rRNA gene of M. parvum in Brazil.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T15:34:43Z
2019-10-06T15:34:43Z
2019-04-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.vetmic.2019.02.024
Veterinary Microbiology, v. 231, p. 33-39.
1873-2542
0378-1135
http://hdl.handle.net/11449/187390
10.1016/j.vetmic.2019.02.024
2-s2.0-85061978352
9139899895580513
url http://dx.doi.org/10.1016/j.vetmic.2019.02.024
http://hdl.handle.net/11449/187390
identifier_str_mv Veterinary Microbiology, v. 231, p. 33-39.
1873-2542
0378-1135
10.1016/j.vetmic.2019.02.024
2-s2.0-85061978352
9139899895580513
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Veterinary Microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 33-39
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128279473815552