Detection of the agr System and Resistance to Antimicrobials in Biofilm-Producing S. epidermidis

Detalhes bibliográficos
Autor(a) principal: Cataneli Pereira, Valéria [UNESP]
Data de Publicação: 2020
Outros Autores: Pinheiro-Hubinger, Luiza [UNESP], de Oliveira, Adilson [UNESP], Moraes Riboli, Danilo Flávio [UNESP], Benini Martins, Katheryne [UNESP], Calixto Romero, Letícia [UNESP], Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/molecules25235715
http://hdl.handle.net/11449/206958
Resumo: The ability of Staphylococcus epidermidis to produce virulence factors, such as biofilm, added to its increased resistance to antimicrobials can cause infections that are difficult to treat. Many staphylococcal virulence factors are under the control of the accessory gene regulator (agr). The objective of this study was to establish the agr locus and susceptibility of biofilm-producing S. epidermidis specimens to antimicrobial agents, through PCR reactions, reverse transcription polymerase chain reaction (RT-PCR), and the determination of minimum inhibitory concentration (MIC), and to analyze the clonal profile of 300 strains isolated from blood culture specimens from inpatients at a University Hospital in Brazil, over a 20-year period by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) techniques. The ica operon expression was shown in 83.6% strains, bhp gene in 11.5%, and aap gene in 32.8%. Oxacillin resistance was detected in 90.1%, while 4.9% showed tigecycline resistance, and intermediate resistance to quinupristin/dalfopristin was identified in 0.4%. Clonal profile determination showed 11 clusters, with the ST2 type determined as the major cluster. The S. epidermidis biofilm producer demonstrated a predominance of agr I locus, oxacillin resistance, and SCCmec III as well as the potential dissemination of pathogenic clones in hospital settings over long periods.
id UNSP_5c3b10024717805ff482ebb6e075397b
oai_identifier_str oai:repositorio.unesp.br:11449/206958
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Detection of the agr System and Resistance to Antimicrobials in Biofilm-Producing S. epidermidisagr locusantimicrobial resistancebiofilmoxacillin resistanceST2The ability of Staphylococcus epidermidis to produce virulence factors, such as biofilm, added to its increased resistance to antimicrobials can cause infections that are difficult to treat. Many staphylococcal virulence factors are under the control of the accessory gene regulator (agr). The objective of this study was to establish the agr locus and susceptibility of biofilm-producing S. epidermidis specimens to antimicrobial agents, through PCR reactions, reverse transcription polymerase chain reaction (RT-PCR), and the determination of minimum inhibitory concentration (MIC), and to analyze the clonal profile of 300 strains isolated from blood culture specimens from inpatients at a University Hospital in Brazil, over a 20-year period by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) techniques. The ica operon expression was shown in 83.6% strains, bhp gene in 11.5%, and aap gene in 32.8%. Oxacillin resistance was detected in 90.1%, while 4.9% showed tigecycline resistance, and intermediate resistance to quinupristin/dalfopristin was identified in 0.4%. Clonal profile determination showed 11 clusters, with the ST2 type determined as the major cluster. The S. epidermidis biofilm producer demonstrated a predominance of agr I locus, oxacillin resistance, and SCCmec III as well as the potential dissemination of pathogenic clones in hospital settings over long periods.Sector of Microbiology and Immunology Department of Chemical and Biological Sciences Institute of Biosciences UNESP-University Estadual PaulistaSector of Microbiology and Immunology UNOESTE-University of West PaulistaSector of Microbiology and Immunology Department of Chemical and Biological Sciences Institute of Biosciences UNESP-University Estadual PaulistaUniversidade Estadual Paulista (Unesp)UNOESTE-University of West PaulistaCataneli Pereira, Valéria [UNESP]Pinheiro-Hubinger, Luiza [UNESP]de Oliveira, Adilson [UNESP]Moraes Riboli, Danilo Flávio [UNESP]Benini Martins, Katheryne [UNESP]Calixto Romero, Letícia [UNESP]Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP]2021-06-25T10:46:44Z2021-06-25T10:46:44Z2020-12-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/molecules25235715Molecules (Basel, Switzerland), v. 25, n. 23, 2020.1420-3049http://hdl.handle.net/11449/20695810.3390/molecules252357152-s2.0-85097483853Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecules (Basel, Switzerland)info:eu-repo/semantics/openAccess2021-10-23T15:48:37Zoai:repositorio.unesp.br:11449/206958Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T15:48:37Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Detection of the agr System and Resistance to Antimicrobials in Biofilm-Producing S. epidermidis
title Detection of the agr System and Resistance to Antimicrobials in Biofilm-Producing S. epidermidis
spellingShingle Detection of the agr System and Resistance to Antimicrobials in Biofilm-Producing S. epidermidis
Cataneli Pereira, Valéria [UNESP]
agr locus
antimicrobial resistance
biofilm
oxacillin resistance
ST2
title_short Detection of the agr System and Resistance to Antimicrobials in Biofilm-Producing S. epidermidis
title_full Detection of the agr System and Resistance to Antimicrobials in Biofilm-Producing S. epidermidis
title_fullStr Detection of the agr System and Resistance to Antimicrobials in Biofilm-Producing S. epidermidis
title_full_unstemmed Detection of the agr System and Resistance to Antimicrobials in Biofilm-Producing S. epidermidis
title_sort Detection of the agr System and Resistance to Antimicrobials in Biofilm-Producing S. epidermidis
author Cataneli Pereira, Valéria [UNESP]
author_facet Cataneli Pereira, Valéria [UNESP]
Pinheiro-Hubinger, Luiza [UNESP]
de Oliveira, Adilson [UNESP]
Moraes Riboli, Danilo Flávio [UNESP]
Benini Martins, Katheryne [UNESP]
Calixto Romero, Letícia [UNESP]
Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP]
author_role author
author2 Pinheiro-Hubinger, Luiza [UNESP]
de Oliveira, Adilson [UNESP]
Moraes Riboli, Danilo Flávio [UNESP]
Benini Martins, Katheryne [UNESP]
Calixto Romero, Letícia [UNESP]
Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
UNOESTE-University of West Paulista
dc.contributor.author.fl_str_mv Cataneli Pereira, Valéria [UNESP]
Pinheiro-Hubinger, Luiza [UNESP]
de Oliveira, Adilson [UNESP]
Moraes Riboli, Danilo Flávio [UNESP]
Benini Martins, Katheryne [UNESP]
Calixto Romero, Letícia [UNESP]
Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP]
dc.subject.por.fl_str_mv agr locus
antimicrobial resistance
biofilm
oxacillin resistance
ST2
topic agr locus
antimicrobial resistance
biofilm
oxacillin resistance
ST2
description The ability of Staphylococcus epidermidis to produce virulence factors, such as biofilm, added to its increased resistance to antimicrobials can cause infections that are difficult to treat. Many staphylococcal virulence factors are under the control of the accessory gene regulator (agr). The objective of this study was to establish the agr locus and susceptibility of biofilm-producing S. epidermidis specimens to antimicrobial agents, through PCR reactions, reverse transcription polymerase chain reaction (RT-PCR), and the determination of minimum inhibitory concentration (MIC), and to analyze the clonal profile of 300 strains isolated from blood culture specimens from inpatients at a University Hospital in Brazil, over a 20-year period by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) techniques. The ica operon expression was shown in 83.6% strains, bhp gene in 11.5%, and aap gene in 32.8%. Oxacillin resistance was detected in 90.1%, while 4.9% showed tigecycline resistance, and intermediate resistance to quinupristin/dalfopristin was identified in 0.4%. Clonal profile determination showed 11 clusters, with the ST2 type determined as the major cluster. The S. epidermidis biofilm producer demonstrated a predominance of agr I locus, oxacillin resistance, and SCCmec III as well as the potential dissemination of pathogenic clones in hospital settings over long periods.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-03
2021-06-25T10:46:44Z
2021-06-25T10:46:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/molecules25235715
Molecules (Basel, Switzerland), v. 25, n. 23, 2020.
1420-3049
http://hdl.handle.net/11449/206958
10.3390/molecules25235715
2-s2.0-85097483853
url http://dx.doi.org/10.3390/molecules25235715
http://hdl.handle.net/11449/206958
identifier_str_mv Molecules (Basel, Switzerland), v. 25, n. 23, 2020.
1420-3049
10.3390/molecules25235715
2-s2.0-85097483853
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Molecules (Basel, Switzerland)
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1799965695352504320