Detection of the agr System and Resistance to Antimicrobials in Biofilm-Producing S. epidermidis
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/molecules25235715 http://hdl.handle.net/11449/206958 |
Resumo: | The ability of Staphylococcus epidermidis to produce virulence factors, such as biofilm, added to its increased resistance to antimicrobials can cause infections that are difficult to treat. Many staphylococcal virulence factors are under the control of the accessory gene regulator (agr). The objective of this study was to establish the agr locus and susceptibility of biofilm-producing S. epidermidis specimens to antimicrobial agents, through PCR reactions, reverse transcription polymerase chain reaction (RT-PCR), and the determination of minimum inhibitory concentration (MIC), and to analyze the clonal profile of 300 strains isolated from blood culture specimens from inpatients at a University Hospital in Brazil, over a 20-year period by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) techniques. The ica operon expression was shown in 83.6% strains, bhp gene in 11.5%, and aap gene in 32.8%. Oxacillin resistance was detected in 90.1%, while 4.9% showed tigecycline resistance, and intermediate resistance to quinupristin/dalfopristin was identified in 0.4%. Clonal profile determination showed 11 clusters, with the ST2 type determined as the major cluster. The S. epidermidis biofilm producer demonstrated a predominance of agr I locus, oxacillin resistance, and SCCmec III as well as the potential dissemination of pathogenic clones in hospital settings over long periods. |
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Detection of the agr System and Resistance to Antimicrobials in Biofilm-Producing S. epidermidisagr locusantimicrobial resistancebiofilmoxacillin resistanceST2The ability of Staphylococcus epidermidis to produce virulence factors, such as biofilm, added to its increased resistance to antimicrobials can cause infections that are difficult to treat. Many staphylococcal virulence factors are under the control of the accessory gene regulator (agr). The objective of this study was to establish the agr locus and susceptibility of biofilm-producing S. epidermidis specimens to antimicrobial agents, through PCR reactions, reverse transcription polymerase chain reaction (RT-PCR), and the determination of minimum inhibitory concentration (MIC), and to analyze the clonal profile of 300 strains isolated from blood culture specimens from inpatients at a University Hospital in Brazil, over a 20-year period by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) techniques. The ica operon expression was shown in 83.6% strains, bhp gene in 11.5%, and aap gene in 32.8%. Oxacillin resistance was detected in 90.1%, while 4.9% showed tigecycline resistance, and intermediate resistance to quinupristin/dalfopristin was identified in 0.4%. Clonal profile determination showed 11 clusters, with the ST2 type determined as the major cluster. The S. epidermidis biofilm producer demonstrated a predominance of agr I locus, oxacillin resistance, and SCCmec III as well as the potential dissemination of pathogenic clones in hospital settings over long periods.Sector of Microbiology and Immunology Department of Chemical and Biological Sciences Institute of Biosciences UNESP-University Estadual PaulistaSector of Microbiology and Immunology UNOESTE-University of West PaulistaSector of Microbiology and Immunology Department of Chemical and Biological Sciences Institute of Biosciences UNESP-University Estadual PaulistaUniversidade Estadual Paulista (Unesp)UNOESTE-University of West PaulistaCataneli Pereira, Valéria [UNESP]Pinheiro-Hubinger, Luiza [UNESP]de Oliveira, Adilson [UNESP]Moraes Riboli, Danilo Flávio [UNESP]Benini Martins, Katheryne [UNESP]Calixto Romero, Letícia [UNESP]Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP]2021-06-25T10:46:44Z2021-06-25T10:46:44Z2020-12-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/molecules25235715Molecules (Basel, Switzerland), v. 25, n. 23, 2020.1420-3049http://hdl.handle.net/11449/20695810.3390/molecules252357152-s2.0-85097483853Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecules (Basel, Switzerland)info:eu-repo/semantics/openAccess2021-10-23T15:48:37Zoai:repositorio.unesp.br:11449/206958Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T15:48:37Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Detection of the agr System and Resistance to Antimicrobials in Biofilm-Producing S. epidermidis |
title |
Detection of the agr System and Resistance to Antimicrobials in Biofilm-Producing S. epidermidis |
spellingShingle |
Detection of the agr System and Resistance to Antimicrobials in Biofilm-Producing S. epidermidis Cataneli Pereira, Valéria [UNESP] agr locus antimicrobial resistance biofilm oxacillin resistance ST2 |
title_short |
Detection of the agr System and Resistance to Antimicrobials in Biofilm-Producing S. epidermidis |
title_full |
Detection of the agr System and Resistance to Antimicrobials in Biofilm-Producing S. epidermidis |
title_fullStr |
Detection of the agr System and Resistance to Antimicrobials in Biofilm-Producing S. epidermidis |
title_full_unstemmed |
Detection of the agr System and Resistance to Antimicrobials in Biofilm-Producing S. epidermidis |
title_sort |
Detection of the agr System and Resistance to Antimicrobials in Biofilm-Producing S. epidermidis |
author |
Cataneli Pereira, Valéria [UNESP] |
author_facet |
Cataneli Pereira, Valéria [UNESP] Pinheiro-Hubinger, Luiza [UNESP] de Oliveira, Adilson [UNESP] Moraes Riboli, Danilo Flávio [UNESP] Benini Martins, Katheryne [UNESP] Calixto Romero, Letícia [UNESP] Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP] |
author_role |
author |
author2 |
Pinheiro-Hubinger, Luiza [UNESP] de Oliveira, Adilson [UNESP] Moraes Riboli, Danilo Flávio [UNESP] Benini Martins, Katheryne [UNESP] Calixto Romero, Letícia [UNESP] Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) UNOESTE-University of West Paulista |
dc.contributor.author.fl_str_mv |
Cataneli Pereira, Valéria [UNESP] Pinheiro-Hubinger, Luiza [UNESP] de Oliveira, Adilson [UNESP] Moraes Riboli, Danilo Flávio [UNESP] Benini Martins, Katheryne [UNESP] Calixto Romero, Letícia [UNESP] Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP] |
dc.subject.por.fl_str_mv |
agr locus antimicrobial resistance biofilm oxacillin resistance ST2 |
topic |
agr locus antimicrobial resistance biofilm oxacillin resistance ST2 |
description |
The ability of Staphylococcus epidermidis to produce virulence factors, such as biofilm, added to its increased resistance to antimicrobials can cause infections that are difficult to treat. Many staphylococcal virulence factors are under the control of the accessory gene regulator (agr). The objective of this study was to establish the agr locus and susceptibility of biofilm-producing S. epidermidis specimens to antimicrobial agents, through PCR reactions, reverse transcription polymerase chain reaction (RT-PCR), and the determination of minimum inhibitory concentration (MIC), and to analyze the clonal profile of 300 strains isolated from blood culture specimens from inpatients at a University Hospital in Brazil, over a 20-year period by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) techniques. The ica operon expression was shown in 83.6% strains, bhp gene in 11.5%, and aap gene in 32.8%. Oxacillin resistance was detected in 90.1%, while 4.9% showed tigecycline resistance, and intermediate resistance to quinupristin/dalfopristin was identified in 0.4%. Clonal profile determination showed 11 clusters, with the ST2 type determined as the major cluster. The S. epidermidis biofilm producer demonstrated a predominance of agr I locus, oxacillin resistance, and SCCmec III as well as the potential dissemination of pathogenic clones in hospital settings over long periods. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-03 2021-06-25T10:46:44Z 2021-06-25T10:46:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/molecules25235715 Molecules (Basel, Switzerland), v. 25, n. 23, 2020. 1420-3049 http://hdl.handle.net/11449/206958 10.3390/molecules25235715 2-s2.0-85097483853 |
url |
http://dx.doi.org/10.3390/molecules25235715 http://hdl.handle.net/11449/206958 |
identifier_str_mv |
Molecules (Basel, Switzerland), v. 25, n. 23, 2020. 1420-3049 10.3390/molecules25235715 2-s2.0-85097483853 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Molecules (Basel, Switzerland) |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965695352504320 |