P-MAPA and IL-12 Differentially Regulate Proteins Associated with Ovarian Cancer Progression: A Proteomic Study
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1021/acsomega.9b02512 http://hdl.handle.net/11449/199836 |
Resumo: | To investigate the potential role of immunotherapies in the cellular and molecular mechanisms associated with ovarian cancer (OC), we applied a comparative proteomic toll using protein identification combined with mass spectrometry. Herein, the effects of the protein aggregate magnesium-ammonium phospholinoleate-palmitoleate anhydride, known as P-MAPA, and the human recombinant interleukin-12 (hrIL-12) were tested alone or in combination in human SKOV-3 cells. The doses and period were defined based on a previous study, which showed that 25 μg/mL P-MAPA and 1 ng/mL IL-12 are sufficient to reduce cell metabolism after 48 h. Indeed, among 2,881 proteins modulated by the treatments, 532 of them were strictly concordant and common. P-MAPA therapy upregulated proteins involved in tight junction, focal adhesion, ribosome constitution, GTP hydrolysis, semaphorin interactions, and expression of SLIT and ROBO, whereas it downregulated ERBB4 signaling, toll-like receptor signaling, regulation of NOTCH 4, and the ubiquitin proteasome pathway. In addition, IL-12 therapy led to upregulation of leukocyte migration, tight junction, and cell signaling, while cell communication, cell metabolism, and Wnt signaling were significantly downregulated in OC cells. A clear majority of proteins that were overexpressed by the combination of P-MAPA with IL-12 are involved in tight junction, focal adhesion, DNA methylation, metabolism of RNA, and ribosomal function; only a small number of downregulated proteins were involved in cell signaling, energy and mitochondrial processes, cell oxidation and senescence, and Wnt signaling. These findings suggest that P-MAPA and IL-12 efficiently regulated important proteins associated with OC progression; these altered proteins may represent potential targets for OC treatment in addition to its immunoadjuvant effects. |
id |
UNSP_61bded4cd06f62215a992e1daf165b8b |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/199836 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
P-MAPA and IL-12 Differentially Regulate Proteins Associated with Ovarian Cancer Progression: A Proteomic StudyTo investigate the potential role of immunotherapies in the cellular and molecular mechanisms associated with ovarian cancer (OC), we applied a comparative proteomic toll using protein identification combined with mass spectrometry. Herein, the effects of the protein aggregate magnesium-ammonium phospholinoleate-palmitoleate anhydride, known as P-MAPA, and the human recombinant interleukin-12 (hrIL-12) were tested alone or in combination in human SKOV-3 cells. The doses and period were defined based on a previous study, which showed that 25 μg/mL P-MAPA and 1 ng/mL IL-12 are sufficient to reduce cell metabolism after 48 h. Indeed, among 2,881 proteins modulated by the treatments, 532 of them were strictly concordant and common. P-MAPA therapy upregulated proteins involved in tight junction, focal adhesion, ribosome constitution, GTP hydrolysis, semaphorin interactions, and expression of SLIT and ROBO, whereas it downregulated ERBB4 signaling, toll-like receptor signaling, regulation of NOTCH 4, and the ubiquitin proteasome pathway. In addition, IL-12 therapy led to upregulation of leukocyte migration, tight junction, and cell signaling, while cell communication, cell metabolism, and Wnt signaling were significantly downregulated in OC cells. A clear majority of proteins that were overexpressed by the combination of P-MAPA with IL-12 are involved in tight junction, focal adhesion, DNA methylation, metabolism of RNA, and ribosomal function; only a small number of downregulated proteins were involved in cell signaling, energy and mitochondrial processes, cell oxidation and senescence, and Wnt signaling. These findings suggest that P-MAPA and IL-12 efficiently regulated important proteins associated with OC progression; these altered proteins may represent potential targets for OC treatment in addition to its immunoadjuvant effects.Department of Anatomy Institute of BiosciencesCenter for the Study of Venoms and Venomous Animals (CEVAP) UNESP - Universidade Estadual PaulistaFarmabrasilis RandD DivisionDepartment of Structural and Functional Biology UNICAMP - University of CampinasDepartment of Morphology and Pathology Federal University of São CarlosCenter for the Study of Venoms and Venomous Animals (CEVAP) UNESP - Universidade Estadual PaulistaUniversidade Estadual Paulista (Unesp)Farmabrasilis RandD DivisionUniversidade Estadual de Campinas (UNICAMP)Universidade Federal de São Carlos (UFSCar)Júnior, Luiz Antonio LupiCucielo, Maira SmaniottoDomeniconi, Raquel FantinDos Santos, Lucilene Delazari [UNESP]Silveira, Henrique SpaulonciDa Silva Nunes, IseuMartinez, MarceloMartinez, Francisco EduardoFávaro, Wagner JoséChuffa, Luiz Gustavo De Almeida2020-12-12T01:50:36Z2020-12-12T01:50:36Z2019-12-24info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article21761-21777http://dx.doi.org/10.1021/acsomega.9b02512ACS Omega, v. 4, n. 26, p. 21761-21777, 2019.2470-1343http://hdl.handle.net/11449/19983610.1021/acsomega.9b025122-s2.0-8507677051654817565282994690000-0003-2938-010XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengACS Omegainfo:eu-repo/semantics/openAccess2024-04-11T15:28:26Zoai:repositorio.unesp.br:11449/199836Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:30:36.755936Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
P-MAPA and IL-12 Differentially Regulate Proteins Associated with Ovarian Cancer Progression: A Proteomic Study |
title |
P-MAPA and IL-12 Differentially Regulate Proteins Associated with Ovarian Cancer Progression: A Proteomic Study |
spellingShingle |
P-MAPA and IL-12 Differentially Regulate Proteins Associated with Ovarian Cancer Progression: A Proteomic Study Júnior, Luiz Antonio Lupi |
title_short |
P-MAPA and IL-12 Differentially Regulate Proteins Associated with Ovarian Cancer Progression: A Proteomic Study |
title_full |
P-MAPA and IL-12 Differentially Regulate Proteins Associated with Ovarian Cancer Progression: A Proteomic Study |
title_fullStr |
P-MAPA and IL-12 Differentially Regulate Proteins Associated with Ovarian Cancer Progression: A Proteomic Study |
title_full_unstemmed |
P-MAPA and IL-12 Differentially Regulate Proteins Associated with Ovarian Cancer Progression: A Proteomic Study |
title_sort |
P-MAPA and IL-12 Differentially Regulate Proteins Associated with Ovarian Cancer Progression: A Proteomic Study |
author |
Júnior, Luiz Antonio Lupi |
author_facet |
Júnior, Luiz Antonio Lupi Cucielo, Maira Smaniotto Domeniconi, Raquel Fantin Dos Santos, Lucilene Delazari [UNESP] Silveira, Henrique Spaulonci Da Silva Nunes, Iseu Martinez, Marcelo Martinez, Francisco Eduardo Fávaro, Wagner José Chuffa, Luiz Gustavo De Almeida |
author_role |
author |
author2 |
Cucielo, Maira Smaniotto Domeniconi, Raquel Fantin Dos Santos, Lucilene Delazari [UNESP] Silveira, Henrique Spaulonci Da Silva Nunes, Iseu Martinez, Marcelo Martinez, Francisco Eduardo Fávaro, Wagner José Chuffa, Luiz Gustavo De Almeida |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Farmabrasilis RandD Division Universidade Estadual de Campinas (UNICAMP) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Júnior, Luiz Antonio Lupi Cucielo, Maira Smaniotto Domeniconi, Raquel Fantin Dos Santos, Lucilene Delazari [UNESP] Silveira, Henrique Spaulonci Da Silva Nunes, Iseu Martinez, Marcelo Martinez, Francisco Eduardo Fávaro, Wagner José Chuffa, Luiz Gustavo De Almeida |
description |
To investigate the potential role of immunotherapies in the cellular and molecular mechanisms associated with ovarian cancer (OC), we applied a comparative proteomic toll using protein identification combined with mass spectrometry. Herein, the effects of the protein aggregate magnesium-ammonium phospholinoleate-palmitoleate anhydride, known as P-MAPA, and the human recombinant interleukin-12 (hrIL-12) were tested alone or in combination in human SKOV-3 cells. The doses and period were defined based on a previous study, which showed that 25 μg/mL P-MAPA and 1 ng/mL IL-12 are sufficient to reduce cell metabolism after 48 h. Indeed, among 2,881 proteins modulated by the treatments, 532 of them were strictly concordant and common. P-MAPA therapy upregulated proteins involved in tight junction, focal adhesion, ribosome constitution, GTP hydrolysis, semaphorin interactions, and expression of SLIT and ROBO, whereas it downregulated ERBB4 signaling, toll-like receptor signaling, regulation of NOTCH 4, and the ubiquitin proteasome pathway. In addition, IL-12 therapy led to upregulation of leukocyte migration, tight junction, and cell signaling, while cell communication, cell metabolism, and Wnt signaling were significantly downregulated in OC cells. A clear majority of proteins that were overexpressed by the combination of P-MAPA with IL-12 are involved in tight junction, focal adhesion, DNA methylation, metabolism of RNA, and ribosomal function; only a small number of downregulated proteins were involved in cell signaling, energy and mitochondrial processes, cell oxidation and senescence, and Wnt signaling. These findings suggest that P-MAPA and IL-12 efficiently regulated important proteins associated with OC progression; these altered proteins may represent potential targets for OC treatment in addition to its immunoadjuvant effects. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-24 2020-12-12T01:50:36Z 2020-12-12T01:50:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1021/acsomega.9b02512 ACS Omega, v. 4, n. 26, p. 21761-21777, 2019. 2470-1343 http://hdl.handle.net/11449/199836 10.1021/acsomega.9b02512 2-s2.0-85076770516 5481756528299469 0000-0003-2938-010X |
url |
http://dx.doi.org/10.1021/acsomega.9b02512 http://hdl.handle.net/11449/199836 |
identifier_str_mv |
ACS Omega, v. 4, n. 26, p. 21761-21777, 2019. 2470-1343 10.1021/acsomega.9b02512 2-s2.0-85076770516 5481756528299469 0000-0003-2938-010X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
ACS Omega |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
21761-21777 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129079359045632 |