Proteomic analysis reveals differentially expressed proteins in macrophages infected with Leishmania amazonensis or Leishmania major.

Detalhes bibliográficos
Autor(a) principal: Menezes, Juliana Perrone Bezerra de
Data de Publicação: 2013
Outros Autores: Almeida, Taís Fontoura de, Petersen, Antonio Luis de Oliveira Almeida, Guedes, Carlos Eduardo Sampaio, Mota, M. S., Lima, José Geraldo Bomfim, Palma, Luana Carneiro, Buck, Gregory A., Krieger, Marco Aurélio, Probst, Christian M., Veras, Patrícia Sampaio Tavares
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7725
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
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spelling Menezes, Juliana Perrone Bezerra deAlmeida, Taís Fontoura dePetersen, Antonio Luis de Oliveira AlmeidaGuedes, Carlos Eduardo SampaioMota, M. S.Lima, José Geraldo BomfimPalma, Luana CarneiroBuck, Gregory A.Krieger, Marco AurélioProbst, Christian M.Veras, Patrícia Sampaio Tavares2014-05-29T19:29:39Z2014-05-29T19:29:39Z2013MENEZES, J. P. B. et al. Proteomic analysis reveals differentially expressed proteins in macrophages infected with Leishmania amazonensis or Leishmania major. Microbes and Infection, v. 15, n. 8-9, p. 579-591, 2013.1769-714Xhttps://www.arca.fiocruz.br/handle/icict/7725dx.doi.org/10.1016/j.micinf.2013.04.005engElsevier Masson SASProteomic analysis reveals differentially expressed proteins in macrophages infected with Leishmania amazonensis or Leishmania major.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil / Federal University of Bahia. Salvador, Bahia, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil / Federal University of Bahia. Salvador, Bahia, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil / Federal University of Bahia. Salvador, Bahia, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil / Federal University of Bahia. Salvador, Bahia, BrasilCenter for the Study of Biological Complexity, Virginia Commonwealth University. Richmond, VA, USAFundação Oswaldo Cruz. Functional Genomic Laboratory. Carlos Chagas Institute (ICC). Paraná, SC, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilCBA macrophages effectively control Leishmania major infection, yet are permissive to Leishmania amazonensis. Employing a transcriptomic approach, we previously showed the up-regulation of the genes involved in the classical pathway of macrophage activation in resistant mice. However, microarray analyses do not evaluate changes in gene expression that occur after translation. To circumvent this analytical limitation, we employed a proteomics approach to increase our understanding of the modulations that occur during infection and identify novel targets for the control of Leishmania infection. To identify proteins whose expression changes in CBA macrophages infected with L. major or L. amazonensis, protein extracts were obtained and digested and the peptides were characterized using multi-dimensional liquid chromatography coupled with tandem mass spectrometry analyses. A total of 162 proteins were selected as potentially modulated. Using biological network analyses, these proteins were classified as primarily involved in cellular metabolism and grouped into cellular development biological networks. This study is the first to use a proteomics approach to describe the protein modulations involved in cellular metabolism during the initial events of Leishmania-macrophage interaction. Based on these findings, we hypothesize that these differentially expressed proteins likely play a pivotal role in determining the course of infection.Leishmania amazonensisLeishmania majorProteomicsMacrophagesInterações Hospedeiro-PatógenoLeishmania major/imunologiaLeishmania mexicana/imunologiaMacrófagos/químicaMacrófagos/parasitologiaProteoma/análiseAnimaisCromatografia LíquidaFemininoLeishmania major/patogenicidadeMacrófagos/imunologiaMasculinoCamundongosCamundongos Endogâmicos CBAEspectrometria de Massas em Tandeminfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALMenezes JPB Proteomic analysis....pdfMenezes JPB Proteomic analysis....pdfapplication/pdf1459187https://www.arca.fiocruz.br/bitstream/icict/7725/1/Menezes%20JPB%20Proteomic%20analysis....pdf8d7186a26071a6aedf0690a3c922fa6aMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7725/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTMenezes JPB Proteomic analysis....pdf.txtMenezes JPB Proteomic analysis....pdf.txtExtracted texttext/plain61740https://www.arca.fiocruz.br/bitstream/icict/7725/5/Menezes%20JPB%20Proteomic%20analysis....pdf.txt62729174b896ca2601177a4edcc0188dMD55THUMBNAILMenezes JPB Proteomic analysis....pdf.jpgMenezes JPB Proteomic analysis....pdf.jpgGenerated Thumbnailimage/jpeg2242https://www.arca.fiocruz.br/bitstream/icict/7725/4/Menezes%20JPB%20Proteomic%20analysis....pdf.jpgd3072f0ee41fa6ca23ae86c501256816MD54icict/77252023-03-15 14:33:16.979oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:16Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Proteomic analysis reveals differentially expressed proteins in macrophages infected with Leishmania amazonensis or Leishmania major.
title Proteomic analysis reveals differentially expressed proteins in macrophages infected with Leishmania amazonensis or Leishmania major.
spellingShingle Proteomic analysis reveals differentially expressed proteins in macrophages infected with Leishmania amazonensis or Leishmania major.
Menezes, Juliana Perrone Bezerra de
Leishmania amazonensis
Leishmania major
Proteomics
Macrophages
Interações Hospedeiro-Patógeno
Leishmania major/imunologia
Leishmania mexicana/imunologia
Macrófagos/química
Macrófagos/parasitologia
Proteoma/análise
Animais
Cromatografia Líquida
Feminino
Leishmania major/patogenicidade
Macrófagos/imunologia
Masculino
Camundongos
Camundongos Endogâmicos CBA
Espectrometria de Massas em Tandem
title_short Proteomic analysis reveals differentially expressed proteins in macrophages infected with Leishmania amazonensis or Leishmania major.
title_full Proteomic analysis reveals differentially expressed proteins in macrophages infected with Leishmania amazonensis or Leishmania major.
title_fullStr Proteomic analysis reveals differentially expressed proteins in macrophages infected with Leishmania amazonensis or Leishmania major.
title_full_unstemmed Proteomic analysis reveals differentially expressed proteins in macrophages infected with Leishmania amazonensis or Leishmania major.
title_sort Proteomic analysis reveals differentially expressed proteins in macrophages infected with Leishmania amazonensis or Leishmania major.
author Menezes, Juliana Perrone Bezerra de
author_facet Menezes, Juliana Perrone Bezerra de
Almeida, Taís Fontoura de
Petersen, Antonio Luis de Oliveira Almeida
Guedes, Carlos Eduardo Sampaio
Mota, M. S.
Lima, José Geraldo Bomfim
Palma, Luana Carneiro
Buck, Gregory A.
Krieger, Marco Aurélio
Probst, Christian M.
Veras, Patrícia Sampaio Tavares
author_role author
author2 Almeida, Taís Fontoura de
Petersen, Antonio Luis de Oliveira Almeida
Guedes, Carlos Eduardo Sampaio
Mota, M. S.
Lima, José Geraldo Bomfim
Palma, Luana Carneiro
Buck, Gregory A.
Krieger, Marco Aurélio
Probst, Christian M.
Veras, Patrícia Sampaio Tavares
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Menezes, Juliana Perrone Bezerra de
Almeida, Taís Fontoura de
Petersen, Antonio Luis de Oliveira Almeida
Guedes, Carlos Eduardo Sampaio
Mota, M. S.
Lima, José Geraldo Bomfim
Palma, Luana Carneiro
Buck, Gregory A.
Krieger, Marco Aurélio
Probst, Christian M.
Veras, Patrícia Sampaio Tavares
dc.subject.en.pt_BR.fl_str_mv Leishmania amazonensis
Leishmania major
Proteomics
Macrophages
topic Leishmania amazonensis
Leishmania major
Proteomics
Macrophages
Interações Hospedeiro-Patógeno
Leishmania major/imunologia
Leishmania mexicana/imunologia
Macrófagos/química
Macrófagos/parasitologia
Proteoma/análise
Animais
Cromatografia Líquida
Feminino
Leishmania major/patogenicidade
Macrófagos/imunologia
Masculino
Camundongos
Camundongos Endogâmicos CBA
Espectrometria de Massas em Tandem
dc.subject.decs.pt_BR.fl_str_mv Interações Hospedeiro-Patógeno
Leishmania major/imunologia
Leishmania mexicana/imunologia
Macrófagos/química
Macrófagos/parasitologia
Proteoma/análise
Animais
Cromatografia Líquida
Feminino
Leishmania major/patogenicidade
Macrófagos/imunologia
Masculino
Camundongos
Camundongos Endogâmicos CBA
Espectrometria de Massas em Tandem
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-05-29T19:29:39Z
dc.date.available.fl_str_mv 2014-05-29T19:29:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MENEZES, J. P. B. et al. Proteomic analysis reveals differentially expressed proteins in macrophages infected with Leishmania amazonensis or Leishmania major. Microbes and Infection, v. 15, n. 8-9, p. 579-591, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7725
dc.identifier.issn.none.fl_str_mv 1769-714X
dc.identifier.doi.none.fl_str_mv dx.doi.org/10.1016/j.micinf.2013.04.005
identifier_str_mv MENEZES, J. P. B. et al. Proteomic analysis reveals differentially expressed proteins in macrophages infected with Leishmania amazonensis or Leishmania major. Microbes and Infection, v. 15, n. 8-9, p. 579-591, 2013.
1769-714X
dx.doi.org/10.1016/j.micinf.2013.04.005
url https://www.arca.fiocruz.br/handle/icict/7725
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier Masson SAS
publisher.none.fl_str_mv Elsevier Masson SAS
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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