Electrocatalytic nanostructured ferric tannate as platform for enzyme conjugation: Electrochemical determination of phenolic compounds
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.bioelechem.2019.107418 http://hdl.handle.net/11449/198237 |
Resumo: | A shell of nanostructured ferric tannates was spontaneously developed on the surface of naked maghemite nanoparticles (SAMNs, the core) by a simple wet reaction with tannic acid (TA). The as obtained core–shell nanomaterial (SAMN@TA) displays specific electrocatalytic and surface properties, which significantly differ from parent maghemite. Thanks to the known proclivity of TA to interact with proteins, SAMN@TA was proposed as a support for the direct immobilization of an enzyme. A ternary functional nanobioconjugate (SAMN@TA@TvL) was successfully self-assembled by incubating laccase from Trametes versicolor (TvL) and SAMN@TA. The SAMN@TA@TvL hybrid was kinetically characterized with respect to the native enzyme and applied for building an easy-to-use analytical device for the detection of polyphenols. The electrochemical biosensor allowed the determination of polyphenols by square wave voltammetry in mixed water-methanol solutions. The system sensitivity was 868.9 ± 1.9nA µM−1, the LOD was 81 nM and the linearity range was comprised between 100 nM and 10 µM. The proposed approach was successfully applied to detect phenolics in blueberry extracts as real samples. Results suggest that SAMN@TA could be a promising, low cost and versatile tool for the creation of nano-bio-conjugates aimed at the development of new electrochemical sensing platforms. |
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Electrocatalytic nanostructured ferric tannate as platform for enzyme conjugation: Electrochemical determination of phenolic compoundsBiosensorEnzyme immobilizationHybrid nanomaterialsLaccaseMagnetic nanoparticlesPhenolic determinationTannic acidA shell of nanostructured ferric tannates was spontaneously developed on the surface of naked maghemite nanoparticles (SAMNs, the core) by a simple wet reaction with tannic acid (TA). The as obtained core–shell nanomaterial (SAMN@TA) displays specific electrocatalytic and surface properties, which significantly differ from parent maghemite. Thanks to the known proclivity of TA to interact with proteins, SAMN@TA was proposed as a support for the direct immobilization of an enzyme. A ternary functional nanobioconjugate (SAMN@TA@TvL) was successfully self-assembled by incubating laccase from Trametes versicolor (TvL) and SAMN@TA. The SAMN@TA@TvL hybrid was kinetically characterized with respect to the native enzyme and applied for building an easy-to-use analytical device for the detection of polyphenols. The electrochemical biosensor allowed the determination of polyphenols by square wave voltammetry in mixed water-methanol solutions. The system sensitivity was 868.9 ± 1.9nA µM−1, the LOD was 81 nM and the linearity range was comprised between 100 nM and 10 µM. The proposed approach was successfully applied to detect phenolics in blueberry extracts as real samples. Results suggest that SAMN@TA could be a promising, low cost and versatile tool for the creation of nano-bio-conjugates aimed at the development of new electrochemical sensing platforms.Università degli Studi di PadovaMinistero dell’Istruzione, dell’Università e della RicercaRegione del VenetoDepartment of Comparative Biomedicine and Food Science University of Padua, Agripolis – Viale dell'Università 16Department of Biomedical Sciences University of Padova, Via Ugo Bassi 58/BDepartment of Agronomy Food Natural Resources Animals and the Environment University of Padua, Viale dell'Università 16Department of Geosciences University of Padua, Via Gradenigo 6Institute of Bioscience Department of Chemistry and Biochemistry UNESPInstitute of Bioscience Department of Chemistry and Biochemistry UNESPUniversità degli Studi di Padova: DOR1989373Ministero dell’Istruzione, dell’Università e della Ricerca: ECCE AQUARegione del Veneto: POR FESR 2014-2020University of PaduaUniversity of PadovaUniversidade Estadual Paulista (Unesp)Magro, MassimilianoBaratella, DavideColò, VeronicaVallese, FrancescaNicoletto, CarloSantagata, SilviaSambo, PaoloMolinari, SimoneSalviulo, GabriellaVenerando, AndreaBasso, Caroline R. [UNESP]Pedrosa, Valber A. [UNESP]Vianello, Fabio2020-12-12T01:07:17Z2020-12-12T01:07:17Z2020-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.bioelechem.2019.107418Bioelectrochemistry, v. 132.1878-562X1567-5394http://hdl.handle.net/11449/19823710.1016/j.bioelechem.2019.1074182-s2.0-85075991946Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioelectrochemistryinfo:eu-repo/semantics/openAccess2021-10-23T10:02:43Zoai:repositorio.unesp.br:11449/198237Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T10:02:43Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Electrocatalytic nanostructured ferric tannate as platform for enzyme conjugation: Electrochemical determination of phenolic compounds |
title |
Electrocatalytic nanostructured ferric tannate as platform for enzyme conjugation: Electrochemical determination of phenolic compounds |
spellingShingle |
Electrocatalytic nanostructured ferric tannate as platform for enzyme conjugation: Electrochemical determination of phenolic compounds Magro, Massimiliano Biosensor Enzyme immobilization Hybrid nanomaterials Laccase Magnetic nanoparticles Phenolic determination Tannic acid |
title_short |
Electrocatalytic nanostructured ferric tannate as platform for enzyme conjugation: Electrochemical determination of phenolic compounds |
title_full |
Electrocatalytic nanostructured ferric tannate as platform for enzyme conjugation: Electrochemical determination of phenolic compounds |
title_fullStr |
Electrocatalytic nanostructured ferric tannate as platform for enzyme conjugation: Electrochemical determination of phenolic compounds |
title_full_unstemmed |
Electrocatalytic nanostructured ferric tannate as platform for enzyme conjugation: Electrochemical determination of phenolic compounds |
title_sort |
Electrocatalytic nanostructured ferric tannate as platform for enzyme conjugation: Electrochemical determination of phenolic compounds |
author |
Magro, Massimiliano |
author_facet |
Magro, Massimiliano Baratella, Davide Colò, Veronica Vallese, Francesca Nicoletto, Carlo Santagata, Silvia Sambo, Paolo Molinari, Simone Salviulo, Gabriella Venerando, Andrea Basso, Caroline R. [UNESP] Pedrosa, Valber A. [UNESP] Vianello, Fabio |
author_role |
author |
author2 |
Baratella, Davide Colò, Veronica Vallese, Francesca Nicoletto, Carlo Santagata, Silvia Sambo, Paolo Molinari, Simone Salviulo, Gabriella Venerando, Andrea Basso, Caroline R. [UNESP] Pedrosa, Valber A. [UNESP] Vianello, Fabio |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
University of Padua University of Padova Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Magro, Massimiliano Baratella, Davide Colò, Veronica Vallese, Francesca Nicoletto, Carlo Santagata, Silvia Sambo, Paolo Molinari, Simone Salviulo, Gabriella Venerando, Andrea Basso, Caroline R. [UNESP] Pedrosa, Valber A. [UNESP] Vianello, Fabio |
dc.subject.por.fl_str_mv |
Biosensor Enzyme immobilization Hybrid nanomaterials Laccase Magnetic nanoparticles Phenolic determination Tannic acid |
topic |
Biosensor Enzyme immobilization Hybrid nanomaterials Laccase Magnetic nanoparticles Phenolic determination Tannic acid |
description |
A shell of nanostructured ferric tannates was spontaneously developed on the surface of naked maghemite nanoparticles (SAMNs, the core) by a simple wet reaction with tannic acid (TA). The as obtained core–shell nanomaterial (SAMN@TA) displays specific electrocatalytic and surface properties, which significantly differ from parent maghemite. Thanks to the known proclivity of TA to interact with proteins, SAMN@TA was proposed as a support for the direct immobilization of an enzyme. A ternary functional nanobioconjugate (SAMN@TA@TvL) was successfully self-assembled by incubating laccase from Trametes versicolor (TvL) and SAMN@TA. The SAMN@TA@TvL hybrid was kinetically characterized with respect to the native enzyme and applied for building an easy-to-use analytical device for the detection of polyphenols. The electrochemical biosensor allowed the determination of polyphenols by square wave voltammetry in mixed water-methanol solutions. The system sensitivity was 868.9 ± 1.9nA µM−1, the LOD was 81 nM and the linearity range was comprised between 100 nM and 10 µM. The proposed approach was successfully applied to detect phenolics in blueberry extracts as real samples. Results suggest that SAMN@TA could be a promising, low cost and versatile tool for the creation of nano-bio-conjugates aimed at the development of new electrochemical sensing platforms. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:07:17Z 2020-12-12T01:07:17Z 2020-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bioelechem.2019.107418 Bioelectrochemistry, v. 132. 1878-562X 1567-5394 http://hdl.handle.net/11449/198237 10.1016/j.bioelechem.2019.107418 2-s2.0-85075991946 |
url |
http://dx.doi.org/10.1016/j.bioelechem.2019.107418 http://hdl.handle.net/11449/198237 |
identifier_str_mv |
Bioelectrochemistry, v. 132. 1878-562X 1567-5394 10.1016/j.bioelechem.2019.107418 2-s2.0-85075991946 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bioelectrochemistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790308818223104 |