Simulação de um reator batelada para a produção de leite sem lactose utilizando enzima β-Galactosidase livre e imobilizada

• Autor(a) principal: Lupino, João Henrique Barcha
• Data de Publicação: 2022
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/216636
• topic: Enzima; Simulação; Leite sem lactose

Brazilian milk production performs an important role in the economy and nutrition in Brazil. However, half of the world population has lactose intolerance, one of the most common genetic disorders, which is the main reason to people do not consume milk. In this sense, the search for viable, safe, clean and economical processes for the production of lactose-free milk has intensified, requiring mathematical modeling and simulations of this process. Thus, the present work aims to simulate and mathematically model a computational routine for the enzymatic hydrolysis process of lactose, in order to investigate the relation among the variables involved in the reaction process without and with inhibition of the enzyme βgalactosidase and the performance of this process, with the development of different simulated scenarios. At first, the batch time for obtaining lactose-free milk using the free enzyme, with and without inhibition, for a batch reactor was simulated using the Scilab Software. Afterwards, the same procedure was performed using the immobilized enzyme. Kinetic parameters required for the simulations were obtained in papers in scientific literature. Finally, it was possible to observe that the variation of β-galactosidase enzyme concentration changes the batch time of lactose-free milk production and also that different immobilization protocols can change the conformational structure of the enzyme and consequently its activity, but providing advantages, as stability and reusability of the enzymes immobilized in the process, making it economically viable.