Development of a new high-performance liquid chromatographic method for the determination of ceftazidime
Autor(a) principal: | |
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Data de Publicação: | 2008 |
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Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1093/jaoac/91.4.739 http://hdl.handle.net/11449/231845 |
Resumo: | A rapid, accurate, and sensitive high-performance liquid chromatographic (HPLC) method was developed and validated for the determination of ceftazidime in pharmaceuticals. The method validation parameters yielded good results and included range, linearity, precision, accuracy, specificity, and recovery. The excipients in the commercial powder for injection did not interfere with the assay. Reversed-phase chromatography was used for the HPLC separation on a Waters C18 (WAT 054275; Milford, MA) column with methanol-water (70 + 30, v/v) as the mobile phase pumped isocratically at a flow rate of 1.0 mL/min. The effluent was monitored at 245 nm. The calibration graph for ceftazidime was linear from 50.0 to 300.0 μg/mL. The values for interday and intraday precision (relative standard deviation) were <1%. The results obtained by the HPLC method were calculated statistically by analysis of variance. We concluded that the HPLC method is satisfactory for the determination of ceftazidime in the raw material and pharmaceuticals. |
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Development of a new high-performance liquid chromatographic method for the determination of ceftazidimeA rapid, accurate, and sensitive high-performance liquid chromatographic (HPLC) method was developed and validated for the determination of ceftazidime in pharmaceuticals. The method validation parameters yielded good results and included range, linearity, precision, accuracy, specificity, and recovery. The excipients in the commercial powder for injection did not interfere with the assay. Reversed-phase chromatography was used for the HPLC separation on a Waters C18 (WAT 054275; Milford, MA) column with methanol-water (70 + 30, v/v) as the mobile phase pumped isocratically at a flow rate of 1.0 mL/min. The effluent was monitored at 245 nm. The calibration graph for ceftazidime was linear from 50.0 to 300.0 μg/mL. The values for interday and intraday precision (relative standard deviation) were <1%. The results obtained by the HPLC method were calculated statistically by analysis of variance. We concluded that the HPLC method is satisfactory for the determination of ceftazidime in the raw material and pharmaceuticals.Universidade Estadual Paulista Faculdade de Ciências Farmacêuticas Programa de Pós-Graduação em Ciências Farmacêuticas, Rodovia Araraquara-Jaú, km 1, CEP 14801-902, Araraquara-SPUniversidade Estadual Paulista Faculdade de Ciências Farmacêuticas Programa de Pós-Graduação em Ciências Farmacêuticas, Rodovia Araraquara-Jaú, km 1, CEP 14801-902, Araraquara-SPUniversidade Estadual Paulista (UNESP)De Haro Moreno, Andréia [UNESP]Salgado, Hérida Regina Nunes [UNESP]2022-04-29T08:47:47Z2022-04-29T08:47:47Z2008-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article739-743http://dx.doi.org/10.1093/jaoac/91.4.739Journal of AOAC International, v. 91, n. 4, p. 739-743, 2008.1060-3271http://hdl.handle.net/11449/23184510.1093/jaoac/91.4.7392-s2.0-50649083235Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of AOAC Internationalinfo:eu-repo/semantics/openAccess2024-06-24T13:45:50Zoai:repositorio.unesp.br:11449/231845Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-06-24T13:45:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Development of a new high-performance liquid chromatographic method for the determination of ceftazidime |
title |
Development of a new high-performance liquid chromatographic method for the determination of ceftazidime |
spellingShingle |
Development of a new high-performance liquid chromatographic method for the determination of ceftazidime De Haro Moreno, Andréia [UNESP] |
title_short |
Development of a new high-performance liquid chromatographic method for the determination of ceftazidime |
title_full |
Development of a new high-performance liquid chromatographic method for the determination of ceftazidime |
title_fullStr |
Development of a new high-performance liquid chromatographic method for the determination of ceftazidime |
title_full_unstemmed |
Development of a new high-performance liquid chromatographic method for the determination of ceftazidime |
title_sort |
Development of a new high-performance liquid chromatographic method for the determination of ceftazidime |
author |
De Haro Moreno, Andréia [UNESP] |
author_facet |
De Haro Moreno, Andréia [UNESP] Salgado, Hérida Regina Nunes [UNESP] |
author_role |
author |
author2 |
Salgado, Hérida Regina Nunes [UNESP] |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
De Haro Moreno, Andréia [UNESP] Salgado, Hérida Regina Nunes [UNESP] |
description |
A rapid, accurate, and sensitive high-performance liquid chromatographic (HPLC) method was developed and validated for the determination of ceftazidime in pharmaceuticals. The method validation parameters yielded good results and included range, linearity, precision, accuracy, specificity, and recovery. The excipients in the commercial powder for injection did not interfere with the assay. Reversed-phase chromatography was used for the HPLC separation on a Waters C18 (WAT 054275; Milford, MA) column with methanol-water (70 + 30, v/v) as the mobile phase pumped isocratically at a flow rate of 1.0 mL/min. The effluent was monitored at 245 nm. The calibration graph for ceftazidime was linear from 50.0 to 300.0 μg/mL. The values for interday and intraday precision (relative standard deviation) were <1%. The results obtained by the HPLC method were calculated statistically by analysis of variance. We concluded that the HPLC method is satisfactory for the determination of ceftazidime in the raw material and pharmaceuticals. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-01 2022-04-29T08:47:47Z 2022-04-29T08:47:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1093/jaoac/91.4.739 Journal of AOAC International, v. 91, n. 4, p. 739-743, 2008. 1060-3271 http://hdl.handle.net/11449/231845 10.1093/jaoac/91.4.739 2-s2.0-50649083235 |
url |
http://dx.doi.org/10.1093/jaoac/91.4.739 http://hdl.handle.net/11449/231845 |
identifier_str_mv |
Journal of AOAC International, v. 91, n. 4, p. 739-743, 2008. 1060-3271 10.1093/jaoac/91.4.739 2-s2.0-50649083235 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of AOAC International |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
739-743 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1803045395626983424 |