Loss of cytokeratin 10 indicates malignant transformation in actinic cheilitis
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00784-015-1557-6 http://hdl.handle.net/11449/161435 |
Resumo: | The aim of this study was to investigate the relationship the expression of cytokeratins (CK10 and CK13) and the cell proliferation index determined by Ki-67 of lip squamous cell carcinoma and actinic cheilitis with different degrees of dysplasia. Forty-five paraffin-embedded actinic cheilitis with and without dysplasia and 20 lip squamous cell carcinoma were analyzed by immunohistochemistry using anti-human anti-CK10, anti-CK13, and anti-Ki-67 antibodies. The majority of actinic cheilitis showed immunopositivity for CK10 and CK13 with decrease or loss of expression in dysplastic areas. In lip squamous cell carcinoma of the lip, heterogeneous expression of CK13 and immunonegativity for CK10 were observed. There was a statistically significant difference between CK10 expression in lip squamous cell carcinoma and in actinic cheilitis with or without dysplasia (p < 0.001). The cell proliferation index was higher in actinic cheilitis with dysplasia and lip squamous cell carcinoma than in actinic cheilitis without epithelial dysplasia. A significant correlation was found between the intensity of the epithelial dysplasia and the cell proliferation index (p < 0.001). These results provide evidence that there is a downregulation of CK10 expression in dysplastic areas of patients with actinic cheilitis and in those with lip squamous cell carcinoma (LSCC) and that the index of cell proliferation, determined by Ki-67, is directly correlated with the intensity of the epithelial dysplasia. Altogether, these results suggest that CK10 expression and the epithelial cell proliferation index can help to identify malignant transformation in the lip region. |
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Loss of cytokeratin 10 indicates malignant transformation in actinic cheilitisActinic cheilitisEpithelial dysplasiaSquamous cell carcinoma of the lipCell proliferation indexThe aim of this study was to investigate the relationship the expression of cytokeratins (CK10 and CK13) and the cell proliferation index determined by Ki-67 of lip squamous cell carcinoma and actinic cheilitis with different degrees of dysplasia. Forty-five paraffin-embedded actinic cheilitis with and without dysplasia and 20 lip squamous cell carcinoma were analyzed by immunohistochemistry using anti-human anti-CK10, anti-CK13, and anti-Ki-67 antibodies. The majority of actinic cheilitis showed immunopositivity for CK10 and CK13 with decrease or loss of expression in dysplastic areas. In lip squamous cell carcinoma of the lip, heterogeneous expression of CK13 and immunonegativity for CK10 were observed. There was a statistically significant difference between CK10 expression in lip squamous cell carcinoma and in actinic cheilitis with or without dysplasia (p < 0.001). The cell proliferation index was higher in actinic cheilitis with dysplasia and lip squamous cell carcinoma than in actinic cheilitis without epithelial dysplasia. A significant correlation was found between the intensity of the epithelial dysplasia and the cell proliferation index (p < 0.001). These results provide evidence that there is a downregulation of CK10 expression in dysplastic areas of patients with actinic cheilitis and in those with lip squamous cell carcinoma (LSCC) and that the index of cell proliferation, determined by Ki-67, is directly correlated with the intensity of the epithelial dysplasia. Altogether, these results suggest that CK10 expression and the epithelial cell proliferation index can help to identify malignant transformation in the lip region.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Sao Paulo, Bauru Sch Dent, Area Pathol, Dept Stomatol, Al Dr Octavio Pinheiro Brisolla 9-75, BR-17012090 Bauru, SP, BrazilUniv Sao Paulo, Bauru Sch Dent, Dept Community Dent, Bauru, SP, BrazilState Univ Sao Paulo, Botucatu Sch Med, Dept Pathol, Botucatu, SP, BrazilLauro de Souza Lima Inst, Dept Pathol, Bauru, SP, BrazilState Univ Sao Paulo, Botucatu Sch Med, Dept Pathol, Botucatu, SP, BrazilCNPq: 141641/2013-4SpringerUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Lauro de Souza Lima InstGarcia, Natalia GalvaoOliveira, Denise TostesPereira Lauris, Jose RobertoCustodio Domingues, Maria Aparecida [UNESP]Minicucci, Eliana Maria [UNESP]Soares, Cleverson Teixeira2018-11-26T16:32:44Z2018-11-26T16:32:44Z2016-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article745-752application/pdfhttp://dx.doi.org/10.1007/s00784-015-1557-6Clinical Oral Investigations. Heidelberg: Springer Heidelberg, v. 20, n. 4, p. 745-752, 2016.1432-6981http://hdl.handle.net/11449/16143510.1007/s00784-015-1557-6WOS:000374562300013WOS000374562300013.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigations0,986info:eu-repo/semantics/openAccess2024-09-03T13:14:54Zoai:repositorio.unesp.br:11449/161435Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:14:54Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Loss of cytokeratin 10 indicates malignant transformation in actinic cheilitis |
title |
Loss of cytokeratin 10 indicates malignant transformation in actinic cheilitis |
spellingShingle |
Loss of cytokeratin 10 indicates malignant transformation in actinic cheilitis Garcia, Natalia Galvao Actinic cheilitis Epithelial dysplasia Squamous cell carcinoma of the lip Cell proliferation index |
title_short |
Loss of cytokeratin 10 indicates malignant transformation in actinic cheilitis |
title_full |
Loss of cytokeratin 10 indicates malignant transformation in actinic cheilitis |
title_fullStr |
Loss of cytokeratin 10 indicates malignant transformation in actinic cheilitis |
title_full_unstemmed |
Loss of cytokeratin 10 indicates malignant transformation in actinic cheilitis |
title_sort |
Loss of cytokeratin 10 indicates malignant transformation in actinic cheilitis |
author |
Garcia, Natalia Galvao |
author_facet |
Garcia, Natalia Galvao Oliveira, Denise Tostes Pereira Lauris, Jose Roberto Custodio Domingues, Maria Aparecida [UNESP] Minicucci, Eliana Maria [UNESP] Soares, Cleverson Teixeira |
author_role |
author |
author2 |
Oliveira, Denise Tostes Pereira Lauris, Jose Roberto Custodio Domingues, Maria Aparecida [UNESP] Minicucci, Eliana Maria [UNESP] Soares, Cleverson Teixeira |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Lauro de Souza Lima Inst |
dc.contributor.author.fl_str_mv |
Garcia, Natalia Galvao Oliveira, Denise Tostes Pereira Lauris, Jose Roberto Custodio Domingues, Maria Aparecida [UNESP] Minicucci, Eliana Maria [UNESP] Soares, Cleverson Teixeira |
dc.subject.por.fl_str_mv |
Actinic cheilitis Epithelial dysplasia Squamous cell carcinoma of the lip Cell proliferation index |
topic |
Actinic cheilitis Epithelial dysplasia Squamous cell carcinoma of the lip Cell proliferation index |
description |
The aim of this study was to investigate the relationship the expression of cytokeratins (CK10 and CK13) and the cell proliferation index determined by Ki-67 of lip squamous cell carcinoma and actinic cheilitis with different degrees of dysplasia. Forty-five paraffin-embedded actinic cheilitis with and without dysplasia and 20 lip squamous cell carcinoma were analyzed by immunohistochemistry using anti-human anti-CK10, anti-CK13, and anti-Ki-67 antibodies. The majority of actinic cheilitis showed immunopositivity for CK10 and CK13 with decrease or loss of expression in dysplastic areas. In lip squamous cell carcinoma of the lip, heterogeneous expression of CK13 and immunonegativity for CK10 were observed. There was a statistically significant difference between CK10 expression in lip squamous cell carcinoma and in actinic cheilitis with or without dysplasia (p < 0.001). The cell proliferation index was higher in actinic cheilitis with dysplasia and lip squamous cell carcinoma than in actinic cheilitis without epithelial dysplasia. A significant correlation was found between the intensity of the epithelial dysplasia and the cell proliferation index (p < 0.001). These results provide evidence that there is a downregulation of CK10 expression in dysplastic areas of patients with actinic cheilitis and in those with lip squamous cell carcinoma (LSCC) and that the index of cell proliferation, determined by Ki-67, is directly correlated with the intensity of the epithelial dysplasia. Altogether, these results suggest that CK10 expression and the epithelial cell proliferation index can help to identify malignant transformation in the lip region. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-05-01 2018-11-26T16:32:44Z 2018-11-26T16:32:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00784-015-1557-6 Clinical Oral Investigations. Heidelberg: Springer Heidelberg, v. 20, n. 4, p. 745-752, 2016. 1432-6981 http://hdl.handle.net/11449/161435 10.1007/s00784-015-1557-6 WOS:000374562300013 WOS000374562300013.pdf |
url |
http://dx.doi.org/10.1007/s00784-015-1557-6 http://hdl.handle.net/11449/161435 |
identifier_str_mv |
Clinical Oral Investigations. Heidelberg: Springer Heidelberg, v. 20, n. 4, p. 745-752, 2016. 1432-6981 10.1007/s00784-015-1557-6 WOS:000374562300013 WOS000374562300013.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Clinical Oral Investigations 0,986 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
745-752 application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021377064828928 |