Loss of cytokeratin 10 indicates malignant transformation in actinic cheilitis

Detalhes bibliográficos
Autor(a) principal: Garcia, Natalia Galvao
Data de Publicação: 2016
Outros Autores: Oliveira, Denise Tostes, Pereira Lauris, Jose Roberto, Custodio Domingues, Maria Aparecida [UNESP], Minicucci, Eliana Maria [UNESP], Soares, Cleverson Teixeira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s00784-015-1557-6
http://hdl.handle.net/11449/161435
Resumo: The aim of this study was to investigate the relationship the expression of cytokeratins (CK10 and CK13) and the cell proliferation index determined by Ki-67 of lip squamous cell carcinoma and actinic cheilitis with different degrees of dysplasia. Forty-five paraffin-embedded actinic cheilitis with and without dysplasia and 20 lip squamous cell carcinoma were analyzed by immunohistochemistry using anti-human anti-CK10, anti-CK13, and anti-Ki-67 antibodies. The majority of actinic cheilitis showed immunopositivity for CK10 and CK13 with decrease or loss of expression in dysplastic areas. In lip squamous cell carcinoma of the lip, heterogeneous expression of CK13 and immunonegativity for CK10 were observed. There was a statistically significant difference between CK10 expression in lip squamous cell carcinoma and in actinic cheilitis with or without dysplasia (p < 0.001). The cell proliferation index was higher in actinic cheilitis with dysplasia and lip squamous cell carcinoma than in actinic cheilitis without epithelial dysplasia. A significant correlation was found between the intensity of the epithelial dysplasia and the cell proliferation index (p < 0.001). These results provide evidence that there is a downregulation of CK10 expression in dysplastic areas of patients with actinic cheilitis and in those with lip squamous cell carcinoma (LSCC) and that the index of cell proliferation, determined by Ki-67, is directly correlated with the intensity of the epithelial dysplasia. Altogether, these results suggest that CK10 expression and the epithelial cell proliferation index can help to identify malignant transformation in the lip region.
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spelling Loss of cytokeratin 10 indicates malignant transformation in actinic cheilitisActinic cheilitisEpithelial dysplasiaSquamous cell carcinoma of the lipCell proliferation indexThe aim of this study was to investigate the relationship the expression of cytokeratins (CK10 and CK13) and the cell proliferation index determined by Ki-67 of lip squamous cell carcinoma and actinic cheilitis with different degrees of dysplasia. Forty-five paraffin-embedded actinic cheilitis with and without dysplasia and 20 lip squamous cell carcinoma were analyzed by immunohistochemistry using anti-human anti-CK10, anti-CK13, and anti-Ki-67 antibodies. The majority of actinic cheilitis showed immunopositivity for CK10 and CK13 with decrease or loss of expression in dysplastic areas. In lip squamous cell carcinoma of the lip, heterogeneous expression of CK13 and immunonegativity for CK10 were observed. There was a statistically significant difference between CK10 expression in lip squamous cell carcinoma and in actinic cheilitis with or without dysplasia (p < 0.001). The cell proliferation index was higher in actinic cheilitis with dysplasia and lip squamous cell carcinoma than in actinic cheilitis without epithelial dysplasia. A significant correlation was found between the intensity of the epithelial dysplasia and the cell proliferation index (p < 0.001). These results provide evidence that there is a downregulation of CK10 expression in dysplastic areas of patients with actinic cheilitis and in those with lip squamous cell carcinoma (LSCC) and that the index of cell proliferation, determined by Ki-67, is directly correlated with the intensity of the epithelial dysplasia. Altogether, these results suggest that CK10 expression and the epithelial cell proliferation index can help to identify malignant transformation in the lip region.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Sao Paulo, Bauru Sch Dent, Area Pathol, Dept Stomatol, Al Dr Octavio Pinheiro Brisolla 9-75, BR-17012090 Bauru, SP, BrazilUniv Sao Paulo, Bauru Sch Dent, Dept Community Dent, Bauru, SP, BrazilState Univ Sao Paulo, Botucatu Sch Med, Dept Pathol, Botucatu, SP, BrazilLauro de Souza Lima Inst, Dept Pathol, Bauru, SP, BrazilState Univ Sao Paulo, Botucatu Sch Med, Dept Pathol, Botucatu, SP, BrazilCNPq: 141641/2013-4SpringerUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Lauro de Souza Lima InstGarcia, Natalia GalvaoOliveira, Denise TostesPereira Lauris, Jose RobertoCustodio Domingues, Maria Aparecida [UNESP]Minicucci, Eliana Maria [UNESP]Soares, Cleverson Teixeira2018-11-26T16:32:44Z2018-11-26T16:32:44Z2016-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article745-752application/pdfhttp://dx.doi.org/10.1007/s00784-015-1557-6Clinical Oral Investigations. Heidelberg: Springer Heidelberg, v. 20, n. 4, p. 745-752, 2016.1432-6981http://hdl.handle.net/11449/16143510.1007/s00784-015-1557-6WOS:000374562300013WOS000374562300013.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigations0,986info:eu-repo/semantics/openAccess2024-09-03T13:14:54Zoai:repositorio.unesp.br:11449/161435Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:14:54Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Loss of cytokeratin 10 indicates malignant transformation in actinic cheilitis
title Loss of cytokeratin 10 indicates malignant transformation in actinic cheilitis
spellingShingle Loss of cytokeratin 10 indicates malignant transformation in actinic cheilitis
Garcia, Natalia Galvao
Actinic cheilitis
Epithelial dysplasia
Squamous cell carcinoma of the lip
Cell proliferation index
title_short Loss of cytokeratin 10 indicates malignant transformation in actinic cheilitis
title_full Loss of cytokeratin 10 indicates malignant transformation in actinic cheilitis
title_fullStr Loss of cytokeratin 10 indicates malignant transformation in actinic cheilitis
title_full_unstemmed Loss of cytokeratin 10 indicates malignant transformation in actinic cheilitis
title_sort Loss of cytokeratin 10 indicates malignant transformation in actinic cheilitis
author Garcia, Natalia Galvao
author_facet Garcia, Natalia Galvao
Oliveira, Denise Tostes
Pereira Lauris, Jose Roberto
Custodio Domingues, Maria Aparecida [UNESP]
Minicucci, Eliana Maria [UNESP]
Soares, Cleverson Teixeira
author_role author
author2 Oliveira, Denise Tostes
Pereira Lauris, Jose Roberto
Custodio Domingues, Maria Aparecida [UNESP]
Minicucci, Eliana Maria [UNESP]
Soares, Cleverson Teixeira
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
Lauro de Souza Lima Inst
dc.contributor.author.fl_str_mv Garcia, Natalia Galvao
Oliveira, Denise Tostes
Pereira Lauris, Jose Roberto
Custodio Domingues, Maria Aparecida [UNESP]
Minicucci, Eliana Maria [UNESP]
Soares, Cleverson Teixeira
dc.subject.por.fl_str_mv Actinic cheilitis
Epithelial dysplasia
Squamous cell carcinoma of the lip
Cell proliferation index
topic Actinic cheilitis
Epithelial dysplasia
Squamous cell carcinoma of the lip
Cell proliferation index
description The aim of this study was to investigate the relationship the expression of cytokeratins (CK10 and CK13) and the cell proliferation index determined by Ki-67 of lip squamous cell carcinoma and actinic cheilitis with different degrees of dysplasia. Forty-five paraffin-embedded actinic cheilitis with and without dysplasia and 20 lip squamous cell carcinoma were analyzed by immunohistochemistry using anti-human anti-CK10, anti-CK13, and anti-Ki-67 antibodies. The majority of actinic cheilitis showed immunopositivity for CK10 and CK13 with decrease or loss of expression in dysplastic areas. In lip squamous cell carcinoma of the lip, heterogeneous expression of CK13 and immunonegativity for CK10 were observed. There was a statistically significant difference between CK10 expression in lip squamous cell carcinoma and in actinic cheilitis with or without dysplasia (p < 0.001). The cell proliferation index was higher in actinic cheilitis with dysplasia and lip squamous cell carcinoma than in actinic cheilitis without epithelial dysplasia. A significant correlation was found between the intensity of the epithelial dysplasia and the cell proliferation index (p < 0.001). These results provide evidence that there is a downregulation of CK10 expression in dysplastic areas of patients with actinic cheilitis and in those with lip squamous cell carcinoma (LSCC) and that the index of cell proliferation, determined by Ki-67, is directly correlated with the intensity of the epithelial dysplasia. Altogether, these results suggest that CK10 expression and the epithelial cell proliferation index can help to identify malignant transformation in the lip region.
publishDate 2016
dc.date.none.fl_str_mv 2016-05-01
2018-11-26T16:32:44Z
2018-11-26T16:32:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00784-015-1557-6
Clinical Oral Investigations. Heidelberg: Springer Heidelberg, v. 20, n. 4, p. 745-752, 2016.
1432-6981
http://hdl.handle.net/11449/161435
10.1007/s00784-015-1557-6
WOS:000374562300013
WOS000374562300013.pdf
url http://dx.doi.org/10.1007/s00784-015-1557-6
http://hdl.handle.net/11449/161435
identifier_str_mv Clinical Oral Investigations. Heidelberg: Springer Heidelberg, v. 20, n. 4, p. 745-752, 2016.
1432-6981
10.1007/s00784-015-1557-6
WOS:000374562300013
WOS000374562300013.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Clinical Oral Investigations
0,986
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 745-752
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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