Comparison of the biocompatibility of different root canal irrigants
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1678-77572008000200011 http://hdl.handle.net/11449/225103 |
Resumo: | The purpose of this study was to compare the reaction of rat subcutaneous connective tissue to 0.9% sterile saline, 2.5% sodium hypochlorite (NaOCl), 5.25% NaOCl and 2% chlorhexidine gluconate solution or gel. Six circles were demarcated on the dorsal skin of 24 male Wistar rats, leaving 2 cm between each circle. Using a syringe, 0.1 mL of each root canal irrigant was injected subcutaneously into 5 circles. In the 6th circle, the needle of an empty syringe was introduced into the skin, but no irrigant was injected (control group). Evaluations were undertaken at 2 h, 48 h, 14 days and 30 days post-procedure. Tissue samples were excised, embedded in paraffin blocks and 3-μm-thick sections were obtained and stained with hematoxylin and eosin. The areas of inflammatory reaction were evaluated and analyzed statistically by ANOVA and Tukey's test. The control group showed few or no inflammatory reaction areas in the subcutaneous tissue. 0.9% saline solution, 2.0% chlorhexidine solution and 2.5% NaOCl showed a good biocompatibility, as very mild inflammatory reaction was detected at 14 days and tissue repair occurred at 30 days. 5.25% NaOCl was the most toxic irrigant, as the number of inflammatory cells remained elevated at 14 and 30 days. The group treated with 2.0% chlorhexidine gluconate gel presented a moderate inflammatory response at 14 days, which decreased at 30 days, being considered similar to that of the control group, 0.9% saline solution, 2.0% chlorhexidine solution and 2.5% NaOCl at this experimental period. |
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Comparison of the biocompatibility of different root canal irrigantsBiocompatibilityChlorhexidineRoot canal irrigantsSodium hypochloriteThe purpose of this study was to compare the reaction of rat subcutaneous connective tissue to 0.9% sterile saline, 2.5% sodium hypochlorite (NaOCl), 5.25% NaOCl and 2% chlorhexidine gluconate solution or gel. Six circles were demarcated on the dorsal skin of 24 male Wistar rats, leaving 2 cm between each circle. Using a syringe, 0.1 mL of each root canal irrigant was injected subcutaneously into 5 circles. In the 6th circle, the needle of an empty syringe was introduced into the skin, but no irrigant was injected (control group). Evaluations were undertaken at 2 h, 48 h, 14 days and 30 days post-procedure. Tissue samples were excised, embedded in paraffin blocks and 3-μm-thick sections were obtained and stained with hematoxylin and eosin. The areas of inflammatory reaction were evaluated and analyzed statistically by ANOVA and Tukey's test. The control group showed few or no inflammatory reaction areas in the subcutaneous tissue. 0.9% saline solution, 2.0% chlorhexidine solution and 2.5% NaOCl showed a good biocompatibility, as very mild inflammatory reaction was detected at 14 days and tissue repair occurred at 30 days. 5.25% NaOCl was the most toxic irrigant, as the number of inflammatory cells remained elevated at 14 and 30 days. The group treated with 2.0% chlorhexidine gluconate gel presented a moderate inflammatory response at 14 days, which decreased at 30 days, being considered similar to that of the control group, 0.9% saline solution, 2.0% chlorhexidine solution and 2.5% NaOCl at this experimental period.Department of Restorative Dentistry Dental School of Araçatuba São Paulo State University, Araçatuba, SPDepartment of Restorative Dentistry School of Dentistry São Paulo State University, Rua José Bonifácio, 1193, 16015-050 Araçatuba, SPDepartment of Restorative Dentistry Dental School of Araçatuba São Paulo State University, Araçatuba, SPDepartment of Restorative Dentistry School of Dentistry São Paulo State University, Rua José Bonifácio, 1193, 16015-050 Araçatuba, SPUniversidade Estadual Paulista (UNESP)Gomes-Filho, João Eduardo [UNESP]Aurélio, Kamila Guilherme [UNESP]Costa, Mariana Machado Teixeira De Moraes [UNESP]Bernabé, Pedro Felício Estrada [UNESP]2022-04-28T20:39:38Z2022-04-28T20:39:38Z2008-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article137-144http://dx.doi.org/10.1590/S1678-77572008000200011Journal of Applied Oral Science, v. 16, n. 2, p. 137-144, 2008.1678-77651678-7757http://hdl.handle.net/11449/22510310.1590/S1678-775720080002000112-s2.0-42049105515Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Applied Oral Scienceinfo:eu-repo/semantics/openAccess2024-09-19T18:31:22Zoai:repositorio.unesp.br:11449/225103Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T18:31:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Comparison of the biocompatibility of different root canal irrigants |
title |
Comparison of the biocompatibility of different root canal irrigants |
spellingShingle |
Comparison of the biocompatibility of different root canal irrigants Gomes-Filho, João Eduardo [UNESP] Biocompatibility Chlorhexidine Root canal irrigants Sodium hypochlorite |
title_short |
Comparison of the biocompatibility of different root canal irrigants |
title_full |
Comparison of the biocompatibility of different root canal irrigants |
title_fullStr |
Comparison of the biocompatibility of different root canal irrigants |
title_full_unstemmed |
Comparison of the biocompatibility of different root canal irrigants |
title_sort |
Comparison of the biocompatibility of different root canal irrigants |
author |
Gomes-Filho, João Eduardo [UNESP] |
author_facet |
Gomes-Filho, João Eduardo [UNESP] Aurélio, Kamila Guilherme [UNESP] Costa, Mariana Machado Teixeira De Moraes [UNESP] Bernabé, Pedro Felício Estrada [UNESP] |
author_role |
author |
author2 |
Aurélio, Kamila Guilherme [UNESP] Costa, Mariana Machado Teixeira De Moraes [UNESP] Bernabé, Pedro Felício Estrada [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Gomes-Filho, João Eduardo [UNESP] Aurélio, Kamila Guilherme [UNESP] Costa, Mariana Machado Teixeira De Moraes [UNESP] Bernabé, Pedro Felício Estrada [UNESP] |
dc.subject.por.fl_str_mv |
Biocompatibility Chlorhexidine Root canal irrigants Sodium hypochlorite |
topic |
Biocompatibility Chlorhexidine Root canal irrigants Sodium hypochlorite |
description |
The purpose of this study was to compare the reaction of rat subcutaneous connective tissue to 0.9% sterile saline, 2.5% sodium hypochlorite (NaOCl), 5.25% NaOCl and 2% chlorhexidine gluconate solution or gel. Six circles were demarcated on the dorsal skin of 24 male Wistar rats, leaving 2 cm between each circle. Using a syringe, 0.1 mL of each root canal irrigant was injected subcutaneously into 5 circles. In the 6th circle, the needle of an empty syringe was introduced into the skin, but no irrigant was injected (control group). Evaluations were undertaken at 2 h, 48 h, 14 days and 30 days post-procedure. Tissue samples were excised, embedded in paraffin blocks and 3-μm-thick sections were obtained and stained with hematoxylin and eosin. The areas of inflammatory reaction were evaluated and analyzed statistically by ANOVA and Tukey's test. The control group showed few or no inflammatory reaction areas in the subcutaneous tissue. 0.9% saline solution, 2.0% chlorhexidine solution and 2.5% NaOCl showed a good biocompatibility, as very mild inflammatory reaction was detected at 14 days and tissue repair occurred at 30 days. 5.25% NaOCl was the most toxic irrigant, as the number of inflammatory cells remained elevated at 14 and 30 days. The group treated with 2.0% chlorhexidine gluconate gel presented a moderate inflammatory response at 14 days, which decreased at 30 days, being considered similar to that of the control group, 0.9% saline solution, 2.0% chlorhexidine solution and 2.5% NaOCl at this experimental period. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-01 2022-04-28T20:39:38Z 2022-04-28T20:39:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1678-77572008000200011 Journal of Applied Oral Science, v. 16, n. 2, p. 137-144, 2008. 1678-7765 1678-7757 http://hdl.handle.net/11449/225103 10.1590/S1678-77572008000200011 2-s2.0-42049105515 |
url |
http://dx.doi.org/10.1590/S1678-77572008000200011 http://hdl.handle.net/11449/225103 |
identifier_str_mv |
Journal of Applied Oral Science, v. 16, n. 2, p. 137-144, 2008. 1678-7765 1678-7757 10.1590/S1678-77572008000200011 2-s2.0-42049105515 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Applied Oral Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
137-144 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1813546402004860928 |