Comparison of the biocompatibility of different root canal irrigants

Detalhes bibliográficos
Autor(a) principal: Gomes-Filho, João Eduardo [UNESP]
Data de Publicação: 2008
Outros Autores: Aurélio, Kamila Guilherme [UNESP], Costa, Mariana Machado Teixeira De Moraes [UNESP], Bernabé, Pedro Felício Estrada [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1678-77572008000200011
http://hdl.handle.net/11449/225103
Resumo: The purpose of this study was to compare the reaction of rat subcutaneous connective tissue to 0.9% sterile saline, 2.5% sodium hypochlorite (NaOCl), 5.25% NaOCl and 2% chlorhexidine gluconate solution or gel. Six circles were demarcated on the dorsal skin of 24 male Wistar rats, leaving 2 cm between each circle. Using a syringe, 0.1 mL of each root canal irrigant was injected subcutaneously into 5 circles. In the 6th circle, the needle of an empty syringe was introduced into the skin, but no irrigant was injected (control group). Evaluations were undertaken at 2 h, 48 h, 14 days and 30 days post-procedure. Tissue samples were excised, embedded in paraffin blocks and 3-μm-thick sections were obtained and stained with hematoxylin and eosin. The areas of inflammatory reaction were evaluated and analyzed statistically by ANOVA and Tukey's test. The control group showed few or no inflammatory reaction areas in the subcutaneous tissue. 0.9% saline solution, 2.0% chlorhexidine solution and 2.5% NaOCl showed a good biocompatibility, as very mild inflammatory reaction was detected at 14 days and tissue repair occurred at 30 days. 5.25% NaOCl was the most toxic irrigant, as the number of inflammatory cells remained elevated at 14 and 30 days. The group treated with 2.0% chlorhexidine gluconate gel presented a moderate inflammatory response at 14 days, which decreased at 30 days, being considered similar to that of the control group, 0.9% saline solution, 2.0% chlorhexidine solution and 2.5% NaOCl at this experimental period.
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spelling Comparison of the biocompatibility of different root canal irrigantsBiocompatibilityChlorhexidineRoot canal irrigantsSodium hypochloriteThe purpose of this study was to compare the reaction of rat subcutaneous connective tissue to 0.9% sterile saline, 2.5% sodium hypochlorite (NaOCl), 5.25% NaOCl and 2% chlorhexidine gluconate solution or gel. Six circles were demarcated on the dorsal skin of 24 male Wistar rats, leaving 2 cm between each circle. Using a syringe, 0.1 mL of each root canal irrigant was injected subcutaneously into 5 circles. In the 6th circle, the needle of an empty syringe was introduced into the skin, but no irrigant was injected (control group). Evaluations were undertaken at 2 h, 48 h, 14 days and 30 days post-procedure. Tissue samples were excised, embedded in paraffin blocks and 3-μm-thick sections were obtained and stained with hematoxylin and eosin. The areas of inflammatory reaction were evaluated and analyzed statistically by ANOVA and Tukey's test. The control group showed few or no inflammatory reaction areas in the subcutaneous tissue. 0.9% saline solution, 2.0% chlorhexidine solution and 2.5% NaOCl showed a good biocompatibility, as very mild inflammatory reaction was detected at 14 days and tissue repair occurred at 30 days. 5.25% NaOCl was the most toxic irrigant, as the number of inflammatory cells remained elevated at 14 and 30 days. The group treated with 2.0% chlorhexidine gluconate gel presented a moderate inflammatory response at 14 days, which decreased at 30 days, being considered similar to that of the control group, 0.9% saline solution, 2.0% chlorhexidine solution and 2.5% NaOCl at this experimental period.Department of Restorative Dentistry Dental School of Araçatuba São Paulo State University, Araçatuba, SPDepartment of Restorative Dentistry School of Dentistry São Paulo State University, Rua José Bonifácio, 1193, 16015-050 Araçatuba, SPDepartment of Restorative Dentistry Dental School of Araçatuba São Paulo State University, Araçatuba, SPDepartment of Restorative Dentistry School of Dentistry São Paulo State University, Rua José Bonifácio, 1193, 16015-050 Araçatuba, SPUniversidade Estadual Paulista (UNESP)Gomes-Filho, João Eduardo [UNESP]Aurélio, Kamila Guilherme [UNESP]Costa, Mariana Machado Teixeira De Moraes [UNESP]Bernabé, Pedro Felício Estrada [UNESP]2022-04-28T20:39:38Z2022-04-28T20:39:38Z2008-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article137-144http://dx.doi.org/10.1590/S1678-77572008000200011Journal of Applied Oral Science, v. 16, n. 2, p. 137-144, 2008.1678-77651678-7757http://hdl.handle.net/11449/22510310.1590/S1678-775720080002000112-s2.0-42049105515Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Applied Oral Scienceinfo:eu-repo/semantics/openAccess2024-09-19T18:31:22Zoai:repositorio.unesp.br:11449/225103Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T18:31:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Comparison of the biocompatibility of different root canal irrigants
title Comparison of the biocompatibility of different root canal irrigants
spellingShingle Comparison of the biocompatibility of different root canal irrigants
Gomes-Filho, João Eduardo [UNESP]
Biocompatibility
Chlorhexidine
Root canal irrigants
Sodium hypochlorite
title_short Comparison of the biocompatibility of different root canal irrigants
title_full Comparison of the biocompatibility of different root canal irrigants
title_fullStr Comparison of the biocompatibility of different root canal irrigants
title_full_unstemmed Comparison of the biocompatibility of different root canal irrigants
title_sort Comparison of the biocompatibility of different root canal irrigants
author Gomes-Filho, João Eduardo [UNESP]
author_facet Gomes-Filho, João Eduardo [UNESP]
Aurélio, Kamila Guilherme [UNESP]
Costa, Mariana Machado Teixeira De Moraes [UNESP]
Bernabé, Pedro Felício Estrada [UNESP]
author_role author
author2 Aurélio, Kamila Guilherme [UNESP]
Costa, Mariana Machado Teixeira De Moraes [UNESP]
Bernabé, Pedro Felício Estrada [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Gomes-Filho, João Eduardo [UNESP]
Aurélio, Kamila Guilherme [UNESP]
Costa, Mariana Machado Teixeira De Moraes [UNESP]
Bernabé, Pedro Felício Estrada [UNESP]
dc.subject.por.fl_str_mv Biocompatibility
Chlorhexidine
Root canal irrigants
Sodium hypochlorite
topic Biocompatibility
Chlorhexidine
Root canal irrigants
Sodium hypochlorite
description The purpose of this study was to compare the reaction of rat subcutaneous connective tissue to 0.9% sterile saline, 2.5% sodium hypochlorite (NaOCl), 5.25% NaOCl and 2% chlorhexidine gluconate solution or gel. Six circles were demarcated on the dorsal skin of 24 male Wistar rats, leaving 2 cm between each circle. Using a syringe, 0.1 mL of each root canal irrigant was injected subcutaneously into 5 circles. In the 6th circle, the needle of an empty syringe was introduced into the skin, but no irrigant was injected (control group). Evaluations were undertaken at 2 h, 48 h, 14 days and 30 days post-procedure. Tissue samples were excised, embedded in paraffin blocks and 3-μm-thick sections were obtained and stained with hematoxylin and eosin. The areas of inflammatory reaction were evaluated and analyzed statistically by ANOVA and Tukey's test. The control group showed few or no inflammatory reaction areas in the subcutaneous tissue. 0.9% saline solution, 2.0% chlorhexidine solution and 2.5% NaOCl showed a good biocompatibility, as very mild inflammatory reaction was detected at 14 days and tissue repair occurred at 30 days. 5.25% NaOCl was the most toxic irrigant, as the number of inflammatory cells remained elevated at 14 and 30 days. The group treated with 2.0% chlorhexidine gluconate gel presented a moderate inflammatory response at 14 days, which decreased at 30 days, being considered similar to that of the control group, 0.9% saline solution, 2.0% chlorhexidine solution and 2.5% NaOCl at this experimental period.
publishDate 2008
dc.date.none.fl_str_mv 2008-01-01
2022-04-28T20:39:38Z
2022-04-28T20:39:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1678-77572008000200011
Journal of Applied Oral Science, v. 16, n. 2, p. 137-144, 2008.
1678-7765
1678-7757
http://hdl.handle.net/11449/225103
10.1590/S1678-77572008000200011
2-s2.0-42049105515
url http://dx.doi.org/10.1590/S1678-77572008000200011
http://hdl.handle.net/11449/225103
identifier_str_mv Journal of Applied Oral Science, v. 16, n. 2, p. 137-144, 2008.
1678-7765
1678-7757
10.1590/S1678-77572008000200011
2-s2.0-42049105515
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Applied Oral Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 137-144
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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