Comparison of the biocompatibility of different root canal irrigants
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1678-77572008000200011 http://hdl.handle.net/11449/15603 |
Resumo: | The purpose of this study was to compare the reaction of rat subcutaneous connective tissue to 0.9% sterile saline, 2.5% sodium hypochlorite (NaOCl), 5.25% NaOCl and 2% chlorhexidine gluconate solution or gel. Six circles were demarcated on the dorsal skin of 24 male Wistar rats, leaving 2 cm between each circle. Using a syringe, 0.1 mL of each root canal irrigant was injected subcutaneously into 5 circles. In the 6th circle, the needle of an empty syringe was introduced into the skin, but no irrigant was injected (control group). Evaluations were undertaken at 2 h, 48 h, 14 days and 30 days post-procedure. Tissue samples were excised, embedded in paraffin blocks and 3-mu m-thick sections were obtained and stained with hematoxylin and eosin. The areas of inflammatory reaction were evaluated and analyzed statistically by ANOVA and Tukey's test. The control group showed few or no inflammatory reaction areas in the subcutaneous tissue. 0.9% saline solution, 2.0% chlorhexidine solution and 2.5% NaOCl showed a good biocompatibility, as very mild inflammatory reaction was detected at 14 days and tissue repair occurred at 30 days. 5.25% NaOCl was the most toxic irrigant, as the number of inflammatory cells remained elevated at 14 and 30 days. The group treated with 2.0% chlorhexidine gluconate gel presented a moderate inflammatory response at 14 days, which decreased at 30 days, being considered similar to that of the control group, 0.9% saline solution, 2.0% chlorhexidine solution and 2.5% NaOCl at this experimental period. |
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Comparison of the biocompatibility of different root canal irrigantsroot canal irrigantssodium hypochloritechlorhexidinebiocompatibilityThe purpose of this study was to compare the reaction of rat subcutaneous connective tissue to 0.9% sterile saline, 2.5% sodium hypochlorite (NaOCl), 5.25% NaOCl and 2% chlorhexidine gluconate solution or gel. Six circles were demarcated on the dorsal skin of 24 male Wistar rats, leaving 2 cm between each circle. Using a syringe, 0.1 mL of each root canal irrigant was injected subcutaneously into 5 circles. In the 6th circle, the needle of an empty syringe was introduced into the skin, but no irrigant was injected (control group). Evaluations were undertaken at 2 h, 48 h, 14 days and 30 days post-procedure. Tissue samples were excised, embedded in paraffin blocks and 3-mu m-thick sections were obtained and stained with hematoxylin and eosin. The areas of inflammatory reaction were evaluated and analyzed statistically by ANOVA and Tukey's test. The control group showed few or no inflammatory reaction areas in the subcutaneous tissue. 0.9% saline solution, 2.0% chlorhexidine solution and 2.5% NaOCl showed a good biocompatibility, as very mild inflammatory reaction was detected at 14 days and tissue repair occurred at 30 days. 5.25% NaOCl was the most toxic irrigant, as the number of inflammatory cells remained elevated at 14 and 30 days. The group treated with 2.0% chlorhexidine gluconate gel presented a moderate inflammatory response at 14 days, which decreased at 30 days, being considered similar to that of the control group, 0.9% saline solution, 2.0% chlorhexidine solution and 2.5% NaOCl at this experimental period.São Paulo State Univ, Dent Sch Aracatuba, Dept Restorat Dent, BR-16015050 Aracatuba, SP, BrazilSão Paulo State Univ, Dent Sch Aracatuba, Dept Restorat Dent, BR-16015050 Aracatuba, SP, BrazilUniversidade de São Paulo (USP), Faculdade de Odontologia de BauruUniversidade Estadual Paulista (Unesp)Gomes-Filho, Joao Eduardo [UNESP]Aurelio, Kamila Guilherme [UNESP]Teixeira de Moraes Costa, Mariana Machado [UNESP]Bernabe, Pedro Felicio Estrada [UNESP]2013-09-30T18:31:02Z2014-05-20T13:44:31Z2013-09-30T18:31:02Z2014-05-20T13:44:31Z2008-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article137-144application/pdfhttp://dx.doi.org/10.1590/S1678-77572008000200011Journal of Applied Oral Science. Bauru-sp: Univ São Paulo Fac Odontologia Bauru, v. 16, n. 2, p. 137-144, 2008.1678-7757http://hdl.handle.net/11449/15603S1678-77572008000200011WOS:000256795700011S1678-77572008000200011.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Applied Oral Science1.7090,645info:eu-repo/semantics/openAccess2024-09-19T18:32:00Zoai:repositorio.unesp.br:11449/15603Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T18:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Comparison of the biocompatibility of different root canal irrigants |
title |
Comparison of the biocompatibility of different root canal irrigants |
spellingShingle |
Comparison of the biocompatibility of different root canal irrigants Gomes-Filho, Joao Eduardo [UNESP] root canal irrigants sodium hypochlorite chlorhexidine biocompatibility |
title_short |
Comparison of the biocompatibility of different root canal irrigants |
title_full |
Comparison of the biocompatibility of different root canal irrigants |
title_fullStr |
Comparison of the biocompatibility of different root canal irrigants |
title_full_unstemmed |
Comparison of the biocompatibility of different root canal irrigants |
title_sort |
Comparison of the biocompatibility of different root canal irrigants |
author |
Gomes-Filho, Joao Eduardo [UNESP] |
author_facet |
Gomes-Filho, Joao Eduardo [UNESP] Aurelio, Kamila Guilherme [UNESP] Teixeira de Moraes Costa, Mariana Machado [UNESP] Bernabe, Pedro Felicio Estrada [UNESP] |
author_role |
author |
author2 |
Aurelio, Kamila Guilherme [UNESP] Teixeira de Moraes Costa, Mariana Machado [UNESP] Bernabe, Pedro Felicio Estrada [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Gomes-Filho, Joao Eduardo [UNESP] Aurelio, Kamila Guilherme [UNESP] Teixeira de Moraes Costa, Mariana Machado [UNESP] Bernabe, Pedro Felicio Estrada [UNESP] |
dc.subject.por.fl_str_mv |
root canal irrigants sodium hypochlorite chlorhexidine biocompatibility |
topic |
root canal irrigants sodium hypochlorite chlorhexidine biocompatibility |
description |
The purpose of this study was to compare the reaction of rat subcutaneous connective tissue to 0.9% sterile saline, 2.5% sodium hypochlorite (NaOCl), 5.25% NaOCl and 2% chlorhexidine gluconate solution or gel. Six circles were demarcated on the dorsal skin of 24 male Wistar rats, leaving 2 cm between each circle. Using a syringe, 0.1 mL of each root canal irrigant was injected subcutaneously into 5 circles. In the 6th circle, the needle of an empty syringe was introduced into the skin, but no irrigant was injected (control group). Evaluations were undertaken at 2 h, 48 h, 14 days and 30 days post-procedure. Tissue samples were excised, embedded in paraffin blocks and 3-mu m-thick sections were obtained and stained with hematoxylin and eosin. The areas of inflammatory reaction were evaluated and analyzed statistically by ANOVA and Tukey's test. The control group showed few or no inflammatory reaction areas in the subcutaneous tissue. 0.9% saline solution, 2.0% chlorhexidine solution and 2.5% NaOCl showed a good biocompatibility, as very mild inflammatory reaction was detected at 14 days and tissue repair occurred at 30 days. 5.25% NaOCl was the most toxic irrigant, as the number of inflammatory cells remained elevated at 14 and 30 days. The group treated with 2.0% chlorhexidine gluconate gel presented a moderate inflammatory response at 14 days, which decreased at 30 days, being considered similar to that of the control group, 0.9% saline solution, 2.0% chlorhexidine solution and 2.5% NaOCl at this experimental period. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-03-01 2013-09-30T18:31:02Z 2013-09-30T18:31:02Z 2014-05-20T13:44:31Z 2014-05-20T13:44:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1678-77572008000200011 Journal of Applied Oral Science. Bauru-sp: Univ São Paulo Fac Odontologia Bauru, v. 16, n. 2, p. 137-144, 2008. 1678-7757 http://hdl.handle.net/11449/15603 S1678-77572008000200011 WOS:000256795700011 S1678-77572008000200011.pdf |
url |
http://dx.doi.org/10.1590/S1678-77572008000200011 http://hdl.handle.net/11449/15603 |
identifier_str_mv |
Journal of Applied Oral Science. Bauru-sp: Univ São Paulo Fac Odontologia Bauru, v. 16, n. 2, p. 137-144, 2008. 1678-7757 S1678-77572008000200011 WOS:000256795700011 S1678-77572008000200011.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Applied Oral Science 1.709 0,645 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
137-144 application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo (USP), Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo (USP), Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1813546519800840192 |