Gels containing statherin-derived peptide protect against enamel and dentin erosive tooth wear in vitro

Detalhes bibliográficos
Autor(a) principal: Reis, Fabiana Navas
Data de Publicação: 2023
Outros Autores: Francese, Monique Malta, Silva, Natara Dias Gomes da, Pelá, Vinicius Taioqui, Câmara, João Victor Frazão, Trevizol, Juliana Sanches, Pieretti, Joana Claudio, Seabra, Amedea Barozzi, Pessan, Juliano Pelim [UNESP], Honório, Heitor Marques, Crusca, Edson [UNESP], Marchetto, Reinaldo [UNESP], Buzalaf, Marília Afonso Rabelo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.jmbbm.2022.105549
http://hdl.handle.net/11449/247849
Resumo: The effect of gels containing a statherin-derived peptide (Stn) on the protection against enamel and dentin erosive tooth wear (ETW) in vitro was evaluated. Bovine enamel and dentin specimens were divided into 2 groups (n = 15 and 18/group for enamel and dentin, respectively) that were treated with Chitosan or Carboxymethylcellulose (CMC) gels containing Stn15pSpS at 1.88 × 10-5 M or 3.76 × 10-5 M. Chitosan or CMC gels without active ingredients served as negative controls, while chitosan gel containing 1.23% F (as NaF) and acidulated phosphate fluoride gel (1.23% F) served as positive controls. The gels were applied on the specimens for 4 min. Stimulated saliva was collected from 3 donors and used to form a 2-h acquired pellicle on the specimens. Then, the specimens were submitted to an erosive pH cycling protocol 4 times/day for 7 days (0.01 M HCl pH 2.0/45 s, artificial saliva/2 h, and artificial saliva overnight). The gels were applied again during pH cycling, 2 times/day for 4 min after the first and last erosive challenges. Enamel and dentin loss (μm) were assessed by contact profilometry. Scanning electron microscopy (SEM) was analyzed using a cold field emission. Data were analyzed by two-way ANOVA (for chitosan and CMC gels, separately) and Tukey's multiple comparison test. SEM images showed changes to enamel topography after application oft the gels containing Stn or F. Regarding CMC-based gels, for enamel, none of the treatments significantly reduced ETW in comparison with placebo; for dentin, however, gels containing Stn, regardless the concentration, significantly reduced the ETW. Moreover, Chitosan-based gels, regardless the Stn concentration, were able to protect enamel and dentin against ETW. Gels containing Stn might be a new approach to protect against ETW.
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spelling Gels containing statherin-derived peptide protect against enamel and dentin erosive tooth wear in vitroDental pellicleHumanStatherin proteinTooth erosionThe effect of gels containing a statherin-derived peptide (Stn) on the protection against enamel and dentin erosive tooth wear (ETW) in vitro was evaluated. Bovine enamel and dentin specimens were divided into 2 groups (n = 15 and 18/group for enamel and dentin, respectively) that were treated with Chitosan or Carboxymethylcellulose (CMC) gels containing Stn15pSpS at 1.88 × 10-5 M or 3.76 × 10-5 M. Chitosan or CMC gels without active ingredients served as negative controls, while chitosan gel containing 1.23% F (as NaF) and acidulated phosphate fluoride gel (1.23% F) served as positive controls. The gels were applied on the specimens for 4 min. Stimulated saliva was collected from 3 donors and used to form a 2-h acquired pellicle on the specimens. Then, the specimens were submitted to an erosive pH cycling protocol 4 times/day for 7 days (0.01 M HCl pH 2.0/45 s, artificial saliva/2 h, and artificial saliva overnight). The gels were applied again during pH cycling, 2 times/day for 4 min after the first and last erosive challenges. Enamel and dentin loss (μm) were assessed by contact profilometry. Scanning electron microscopy (SEM) was analyzed using a cold field emission. Data were analyzed by two-way ANOVA (for chitosan and CMC gels, separately) and Tukey's multiple comparison test. SEM images showed changes to enamel topography after application oft the gels containing Stn or F. Regarding CMC-based gels, for enamel, none of the treatments significantly reduced ETW in comparison with placebo; for dentin, however, gels containing Stn, regardless the concentration, significantly reduced the ETW. Moreover, Chitosan-based gels, regardless the Stn concentration, were able to protect enamel and dentin against ETW. Gels containing Stn might be a new approach to protect against ETW.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Biological Sciences Bauru School of Dentistry University of São PauloCenter for Natural and Human Sciences Federal University of ABCDepartment of Pediatric Dentistry and Public Health Araçatuba Dental School São Paulo State University UNESP, AraçatubaChemistry Institute Paulista State UniversityDepartment of Pediatric Dentistry and Public Health Araçatuba Dental School São Paulo State University UNESP, AraçatubaChemistry Institute Paulista State UniversityFAPESP: 2019/26070–1Universidade de São Paulo (USP)Federal University of ABCUniversidade Estadual Paulista (UNESP)Reis, Fabiana NavasFrancese, Monique MaltaSilva, Natara Dias Gomes daPelá, Vinicius TaioquiCâmara, João Victor FrazãoTrevizol, Juliana SanchesPieretti, Joana ClaudioSeabra, Amedea BarozziPessan, Juliano Pelim [UNESP]Honório, Heitor MarquesCrusca, Edson [UNESP]Marchetto, Reinaldo [UNESP]Buzalaf, Marília Afonso Rabelo2023-07-29T13:27:36Z2023-07-29T13:27:36Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jmbbm.2022.105549Journal of the Mechanical Behavior of Biomedical Materials, v. 137.1878-01801751-6161http://hdl.handle.net/11449/24784910.1016/j.jmbbm.2022.1055492-s2.0-85141529742Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of the Mechanical Behavior of Biomedical Materialsinfo:eu-repo/semantics/openAccess2023-07-29T13:27:37Zoai:repositorio.unesp.br:11449/247849Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T13:27:37Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Gels containing statherin-derived peptide protect against enamel and dentin erosive tooth wear in vitro
title Gels containing statherin-derived peptide protect against enamel and dentin erosive tooth wear in vitro
spellingShingle Gels containing statherin-derived peptide protect against enamel and dentin erosive tooth wear in vitro
Reis, Fabiana Navas
Dental pellicle
Human
Statherin protein
Tooth erosion
title_short Gels containing statherin-derived peptide protect against enamel and dentin erosive tooth wear in vitro
title_full Gels containing statherin-derived peptide protect against enamel and dentin erosive tooth wear in vitro
title_fullStr Gels containing statherin-derived peptide protect against enamel and dentin erosive tooth wear in vitro
title_full_unstemmed Gels containing statherin-derived peptide protect against enamel and dentin erosive tooth wear in vitro
title_sort Gels containing statherin-derived peptide protect against enamel and dentin erosive tooth wear in vitro
author Reis, Fabiana Navas
author_facet Reis, Fabiana Navas
Francese, Monique Malta
Silva, Natara Dias Gomes da
Pelá, Vinicius Taioqui
Câmara, João Victor Frazão
Trevizol, Juliana Sanches
Pieretti, Joana Claudio
Seabra, Amedea Barozzi
Pessan, Juliano Pelim [UNESP]
Honório, Heitor Marques
Crusca, Edson [UNESP]
Marchetto, Reinaldo [UNESP]
Buzalaf, Marília Afonso Rabelo
author_role author
author2 Francese, Monique Malta
Silva, Natara Dias Gomes da
Pelá, Vinicius Taioqui
Câmara, João Victor Frazão
Trevizol, Juliana Sanches
Pieretti, Joana Claudio
Seabra, Amedea Barozzi
Pessan, Juliano Pelim [UNESP]
Honório, Heitor Marques
Crusca, Edson [UNESP]
Marchetto, Reinaldo [UNESP]
Buzalaf, Marília Afonso Rabelo
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Federal University of ABC
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Reis, Fabiana Navas
Francese, Monique Malta
Silva, Natara Dias Gomes da
Pelá, Vinicius Taioqui
Câmara, João Victor Frazão
Trevizol, Juliana Sanches
Pieretti, Joana Claudio
Seabra, Amedea Barozzi
Pessan, Juliano Pelim [UNESP]
Honório, Heitor Marques
Crusca, Edson [UNESP]
Marchetto, Reinaldo [UNESP]
Buzalaf, Marília Afonso Rabelo
dc.subject.por.fl_str_mv Dental pellicle
Human
Statherin protein
Tooth erosion
topic Dental pellicle
Human
Statherin protein
Tooth erosion
description The effect of gels containing a statherin-derived peptide (Stn) on the protection against enamel and dentin erosive tooth wear (ETW) in vitro was evaluated. Bovine enamel and dentin specimens were divided into 2 groups (n = 15 and 18/group for enamel and dentin, respectively) that were treated with Chitosan or Carboxymethylcellulose (CMC) gels containing Stn15pSpS at 1.88 × 10-5 M or 3.76 × 10-5 M. Chitosan or CMC gels without active ingredients served as negative controls, while chitosan gel containing 1.23% F (as NaF) and acidulated phosphate fluoride gel (1.23% F) served as positive controls. The gels were applied on the specimens for 4 min. Stimulated saliva was collected from 3 donors and used to form a 2-h acquired pellicle on the specimens. Then, the specimens were submitted to an erosive pH cycling protocol 4 times/day for 7 days (0.01 M HCl pH 2.0/45 s, artificial saliva/2 h, and artificial saliva overnight). The gels were applied again during pH cycling, 2 times/day for 4 min after the first and last erosive challenges. Enamel and dentin loss (μm) were assessed by contact profilometry. Scanning electron microscopy (SEM) was analyzed using a cold field emission. Data were analyzed by two-way ANOVA (for chitosan and CMC gels, separately) and Tukey's multiple comparison test. SEM images showed changes to enamel topography after application oft the gels containing Stn or F. Regarding CMC-based gels, for enamel, none of the treatments significantly reduced ETW in comparison with placebo; for dentin, however, gels containing Stn, regardless the concentration, significantly reduced the ETW. Moreover, Chitosan-based gels, regardless the Stn concentration, were able to protect enamel and dentin against ETW. Gels containing Stn might be a new approach to protect against ETW.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T13:27:36Z
2023-07-29T13:27:36Z
2023-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.jmbbm.2022.105549
Journal of the Mechanical Behavior of Biomedical Materials, v. 137.
1878-0180
1751-6161
http://hdl.handle.net/11449/247849
10.1016/j.jmbbm.2022.105549
2-s2.0-85141529742
url http://dx.doi.org/10.1016/j.jmbbm.2022.105549
http://hdl.handle.net/11449/247849
identifier_str_mv Journal of the Mechanical Behavior of Biomedical Materials, v. 137.
1878-0180
1751-6161
10.1016/j.jmbbm.2022.105549
2-s2.0-85141529742
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of the Mechanical Behavior of Biomedical Materials
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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