Gels containing statherin-derived peptide protect against enamel and dentin erosive tooth wear in vitro
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jmbbm.2022.105549 http://hdl.handle.net/11449/247849 |
Resumo: | The effect of gels containing a statherin-derived peptide (Stn) on the protection against enamel and dentin erosive tooth wear (ETW) in vitro was evaluated. Bovine enamel and dentin specimens were divided into 2 groups (n = 15 and 18/group for enamel and dentin, respectively) that were treated with Chitosan or Carboxymethylcellulose (CMC) gels containing Stn15pSpS at 1.88 × 10-5 M or 3.76 × 10-5 M. Chitosan or CMC gels without active ingredients served as negative controls, while chitosan gel containing 1.23% F (as NaF) and acidulated phosphate fluoride gel (1.23% F) served as positive controls. The gels were applied on the specimens for 4 min. Stimulated saliva was collected from 3 donors and used to form a 2-h acquired pellicle on the specimens. Then, the specimens were submitted to an erosive pH cycling protocol 4 times/day for 7 days (0.01 M HCl pH 2.0/45 s, artificial saliva/2 h, and artificial saliva overnight). The gels were applied again during pH cycling, 2 times/day for 4 min after the first and last erosive challenges. Enamel and dentin loss (μm) were assessed by contact profilometry. Scanning electron microscopy (SEM) was analyzed using a cold field emission. Data were analyzed by two-way ANOVA (for chitosan and CMC gels, separately) and Tukey's multiple comparison test. SEM images showed changes to enamel topography after application oft the gels containing Stn or F. Regarding CMC-based gels, for enamel, none of the treatments significantly reduced ETW in comparison with placebo; for dentin, however, gels containing Stn, regardless the concentration, significantly reduced the ETW. Moreover, Chitosan-based gels, regardless the Stn concentration, were able to protect enamel and dentin against ETW. Gels containing Stn might be a new approach to protect against ETW. |
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Gels containing statherin-derived peptide protect against enamel and dentin erosive tooth wear in vitroDental pellicleHumanStatherin proteinTooth erosionThe effect of gels containing a statherin-derived peptide (Stn) on the protection against enamel and dentin erosive tooth wear (ETW) in vitro was evaluated. Bovine enamel and dentin specimens were divided into 2 groups (n = 15 and 18/group for enamel and dentin, respectively) that were treated with Chitosan or Carboxymethylcellulose (CMC) gels containing Stn15pSpS at 1.88 × 10-5 M or 3.76 × 10-5 M. Chitosan or CMC gels without active ingredients served as negative controls, while chitosan gel containing 1.23% F (as NaF) and acidulated phosphate fluoride gel (1.23% F) served as positive controls. The gels were applied on the specimens for 4 min. Stimulated saliva was collected from 3 donors and used to form a 2-h acquired pellicle on the specimens. Then, the specimens were submitted to an erosive pH cycling protocol 4 times/day for 7 days (0.01 M HCl pH 2.0/45 s, artificial saliva/2 h, and artificial saliva overnight). The gels were applied again during pH cycling, 2 times/day for 4 min after the first and last erosive challenges. Enamel and dentin loss (μm) were assessed by contact profilometry. Scanning electron microscopy (SEM) was analyzed using a cold field emission. Data were analyzed by two-way ANOVA (for chitosan and CMC gels, separately) and Tukey's multiple comparison test. SEM images showed changes to enamel topography after application oft the gels containing Stn or F. Regarding CMC-based gels, for enamel, none of the treatments significantly reduced ETW in comparison with placebo; for dentin, however, gels containing Stn, regardless the concentration, significantly reduced the ETW. Moreover, Chitosan-based gels, regardless the Stn concentration, were able to protect enamel and dentin against ETW. Gels containing Stn might be a new approach to protect against ETW.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Biological Sciences Bauru School of Dentistry University of São PauloCenter for Natural and Human Sciences Federal University of ABCDepartment of Pediatric Dentistry and Public Health Araçatuba Dental School São Paulo State University UNESP, AraçatubaChemistry Institute Paulista State UniversityDepartment of Pediatric Dentistry and Public Health Araçatuba Dental School São Paulo State University UNESP, AraçatubaChemistry Institute Paulista State UniversityFAPESP: 2019/26070–1Universidade de São Paulo (USP)Federal University of ABCUniversidade Estadual Paulista (UNESP)Reis, Fabiana NavasFrancese, Monique MaltaSilva, Natara Dias Gomes daPelá, Vinicius TaioquiCâmara, João Victor FrazãoTrevizol, Juliana SanchesPieretti, Joana ClaudioSeabra, Amedea BarozziPessan, Juliano Pelim [UNESP]Honório, Heitor MarquesCrusca, Edson [UNESP]Marchetto, Reinaldo [UNESP]Buzalaf, Marília Afonso Rabelo2023-07-29T13:27:36Z2023-07-29T13:27:36Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jmbbm.2022.105549Journal of the Mechanical Behavior of Biomedical Materials, v. 137.1878-01801751-6161http://hdl.handle.net/11449/24784910.1016/j.jmbbm.2022.1055492-s2.0-85141529742Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of the Mechanical Behavior of Biomedical Materialsinfo:eu-repo/semantics/openAccess2023-07-29T13:27:37Zoai:repositorio.unesp.br:11449/247849Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:47:17.012678Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Gels containing statherin-derived peptide protect against enamel and dentin erosive tooth wear in vitro |
title |
Gels containing statherin-derived peptide protect against enamel and dentin erosive tooth wear in vitro |
spellingShingle |
Gels containing statherin-derived peptide protect against enamel and dentin erosive tooth wear in vitro Reis, Fabiana Navas Dental pellicle Human Statherin protein Tooth erosion |
title_short |
Gels containing statherin-derived peptide protect against enamel and dentin erosive tooth wear in vitro |
title_full |
Gels containing statherin-derived peptide protect against enamel and dentin erosive tooth wear in vitro |
title_fullStr |
Gels containing statherin-derived peptide protect against enamel and dentin erosive tooth wear in vitro |
title_full_unstemmed |
Gels containing statherin-derived peptide protect against enamel and dentin erosive tooth wear in vitro |
title_sort |
Gels containing statherin-derived peptide protect against enamel and dentin erosive tooth wear in vitro |
author |
Reis, Fabiana Navas |
author_facet |
Reis, Fabiana Navas Francese, Monique Malta Silva, Natara Dias Gomes da Pelá, Vinicius Taioqui Câmara, João Victor Frazão Trevizol, Juliana Sanches Pieretti, Joana Claudio Seabra, Amedea Barozzi Pessan, Juliano Pelim [UNESP] Honório, Heitor Marques Crusca, Edson [UNESP] Marchetto, Reinaldo [UNESP] Buzalaf, Marília Afonso Rabelo |
author_role |
author |
author2 |
Francese, Monique Malta Silva, Natara Dias Gomes da Pelá, Vinicius Taioqui Câmara, João Victor Frazão Trevizol, Juliana Sanches Pieretti, Joana Claudio Seabra, Amedea Barozzi Pessan, Juliano Pelim [UNESP] Honório, Heitor Marques Crusca, Edson [UNESP] Marchetto, Reinaldo [UNESP] Buzalaf, Marília Afonso Rabelo |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Federal University of ABC Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Reis, Fabiana Navas Francese, Monique Malta Silva, Natara Dias Gomes da Pelá, Vinicius Taioqui Câmara, João Victor Frazão Trevizol, Juliana Sanches Pieretti, Joana Claudio Seabra, Amedea Barozzi Pessan, Juliano Pelim [UNESP] Honório, Heitor Marques Crusca, Edson [UNESP] Marchetto, Reinaldo [UNESP] Buzalaf, Marília Afonso Rabelo |
dc.subject.por.fl_str_mv |
Dental pellicle Human Statherin protein Tooth erosion |
topic |
Dental pellicle Human Statherin protein Tooth erosion |
description |
The effect of gels containing a statherin-derived peptide (Stn) on the protection against enamel and dentin erosive tooth wear (ETW) in vitro was evaluated. Bovine enamel and dentin specimens were divided into 2 groups (n = 15 and 18/group for enamel and dentin, respectively) that were treated with Chitosan or Carboxymethylcellulose (CMC) gels containing Stn15pSpS at 1.88 × 10-5 M or 3.76 × 10-5 M. Chitosan or CMC gels without active ingredients served as negative controls, while chitosan gel containing 1.23% F (as NaF) and acidulated phosphate fluoride gel (1.23% F) served as positive controls. The gels were applied on the specimens for 4 min. Stimulated saliva was collected from 3 donors and used to form a 2-h acquired pellicle on the specimens. Then, the specimens were submitted to an erosive pH cycling protocol 4 times/day for 7 days (0.01 M HCl pH 2.0/45 s, artificial saliva/2 h, and artificial saliva overnight). The gels were applied again during pH cycling, 2 times/day for 4 min after the first and last erosive challenges. Enamel and dentin loss (μm) were assessed by contact profilometry. Scanning electron microscopy (SEM) was analyzed using a cold field emission. Data were analyzed by two-way ANOVA (for chitosan and CMC gels, separately) and Tukey's multiple comparison test. SEM images showed changes to enamel topography after application oft the gels containing Stn or F. Regarding CMC-based gels, for enamel, none of the treatments significantly reduced ETW in comparison with placebo; for dentin, however, gels containing Stn, regardless the concentration, significantly reduced the ETW. Moreover, Chitosan-based gels, regardless the Stn concentration, were able to protect enamel and dentin against ETW. Gels containing Stn might be a new approach to protect against ETW. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:27:36Z 2023-07-29T13:27:36Z 2023-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jmbbm.2022.105549 Journal of the Mechanical Behavior of Biomedical Materials, v. 137. 1878-0180 1751-6161 http://hdl.handle.net/11449/247849 10.1016/j.jmbbm.2022.105549 2-s2.0-85141529742 |
url |
http://dx.doi.org/10.1016/j.jmbbm.2022.105549 http://hdl.handle.net/11449/247849 |
identifier_str_mv |
Journal of the Mechanical Behavior of Biomedical Materials, v. 137. 1878-0180 1751-6161 10.1016/j.jmbbm.2022.105549 2-s2.0-85141529742 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of the Mechanical Behavior of Biomedical Materials |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128858289864704 |