Minimal Enzymes Cocktail Development by Filamentous Fungi Consortia in Solid-State Cultivation and Valorization of Pineapple Crown Waste by Enzymatic Saccharification
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s12649-020-01199-8 http://hdl.handle.net/11449/221532 |
Resumo: | Agroindustrial wastes is an interesting opportunity for enzymes production, reducing operational costs and strengthening the biorefineries concept. The conversion of lignocellulosic waste to fermentable sugars is current challenge for biorefineries industries. Enzyme cocktail used in delignification and saccharification platform are produced by a combinatory of several lignocellulolytic enzymes. The aim of this study was to develop filamentous fungi compatible-consortia isolated from pineapple wastes for cellulose-degrading enzymes production using pineapple crown waste in solid-state cultivation and its potential for saccharification of pineapple crown waste. Isolates were screened in agar-plate and in solid-state cultivation for cellulases, xylanase and pectinases productions. Six compatible consortia of Trichoderma strains with Aspergillus niger or Pleurotus ostreatus were evaluated for SSC using pineapple crown. All consortia increased the enzymes production compared to monoculture. Consortia of Trichoderma asperellum PEC-6 and P. ostreatus increased 1.60-fold the xylanase and 1.42-fold the β-glucosidase productions. T. asperellum PEC-17 and A. niger increased 1.16-fold endoglucanase and 1.28-fold pectinase. Saccharification of pineapple crown waste using enzyme cocktail produced by filamentous fungi consortia increased the total reducing sugar released in 12.50–13.93% compared to enzymes produced by monocultures. This study provides an alternative model to the cocktail enzyme production by mixed cultures development with lower cost of on-site enzyme manufacture by use of agroindustrial wastes eliminating an enzyme blended step. Moreover, the procedure used in this work can be potentially cost saving and environmentally friendly and should be explored on other bioenergy feedstocks and feed production. Graphic Abstract: [Figure not available: see fulltext.] |
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Minimal Enzymes Cocktail Development by Filamentous Fungi Consortia in Solid-State Cultivation and Valorization of Pineapple Crown Waste by Enzymatic Saccharificationbiomass saccharificationEnzyme productionMicrobial consortiaSolid-state cultivationTrichodermaAgroindustrial wastes is an interesting opportunity for enzymes production, reducing operational costs and strengthening the biorefineries concept. The conversion of lignocellulosic waste to fermentable sugars is current challenge for biorefineries industries. Enzyme cocktail used in delignification and saccharification platform are produced by a combinatory of several lignocellulolytic enzymes. The aim of this study was to develop filamentous fungi compatible-consortia isolated from pineapple wastes for cellulose-degrading enzymes production using pineapple crown waste in solid-state cultivation and its potential for saccharification of pineapple crown waste. Isolates were screened in agar-plate and in solid-state cultivation for cellulases, xylanase and pectinases productions. Six compatible consortia of Trichoderma strains with Aspergillus niger or Pleurotus ostreatus were evaluated for SSC using pineapple crown. All consortia increased the enzymes production compared to monoculture. Consortia of Trichoderma asperellum PEC-6 and P. ostreatus increased 1.60-fold the xylanase and 1.42-fold the β-glucosidase productions. T. asperellum PEC-17 and A. niger increased 1.16-fold endoglucanase and 1.28-fold pectinase. Saccharification of pineapple crown waste using enzyme cocktail produced by filamentous fungi consortia increased the total reducing sugar released in 12.50–13.93% compared to enzymes produced by monocultures. This study provides an alternative model to the cocktail enzyme production by mixed cultures development with lower cost of on-site enzyme manufacture by use of agroindustrial wastes eliminating an enzyme blended step. Moreover, the procedure used in this work can be potentially cost saving and environmentally friendly and should be explored on other bioenergy feedstocks and feed production. Graphic Abstract: [Figure not available: see fulltext.]Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Bioprocess Engineering and Biotechnology Federal University of TocantinsPost-Graduate Food Science and Technology Federal University of TocantinsHabite - Biotechnology-Based Incubator Companies Federal University of TocantinsBiochemical and Microbiology Department Univ. Est. PaulistaFederal University of Bahia Vitória da ConquistaEmbrapa AgroenergiaCAPES: Finance Code 001Federal University of TocantinsUniv. Est. PaulistaUniversidade Federal da Bahia (UFBA)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Teixeira, Wéllen Fabrícia AvizBatista, Ryhára Diasdo Amaral Santos, Claudia Cristina AulerJúnior, Aloísio Chagas FreitasTerrasan, César Rafael Fanchinide Santana, Marcio Waluce Pinheiro R.de Siqueira, Félix Gonçalvesde Paula-Elias, Fabrício Coutinhode Almeida, Alex Fernando2022-04-28T19:29:15Z2022-04-28T19:29:15Z2021-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2521-2539http://dx.doi.org/10.1007/s12649-020-01199-8Waste and Biomass Valorization, v. 12, n. 5, p. 2521-2539, 2021.1877-265X1877-2641http://hdl.handle.net/11449/22153210.1007/s12649-020-01199-82-s2.0-85089476777Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengWaste and Biomass Valorizationinfo:eu-repo/semantics/openAccess2022-04-28T19:29:15Zoai:repositorio.unesp.br:11449/221532Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T19:29:15Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Minimal Enzymes Cocktail Development by Filamentous Fungi Consortia in Solid-State Cultivation and Valorization of Pineapple Crown Waste by Enzymatic Saccharification |
title |
Minimal Enzymes Cocktail Development by Filamentous Fungi Consortia in Solid-State Cultivation and Valorization of Pineapple Crown Waste by Enzymatic Saccharification |
spellingShingle |
Minimal Enzymes Cocktail Development by Filamentous Fungi Consortia in Solid-State Cultivation and Valorization of Pineapple Crown Waste by Enzymatic Saccharification Teixeira, Wéllen Fabrícia Aviz biomass saccharification Enzyme production Microbial consortia Solid-state cultivation Trichoderma |
title_short |
Minimal Enzymes Cocktail Development by Filamentous Fungi Consortia in Solid-State Cultivation and Valorization of Pineapple Crown Waste by Enzymatic Saccharification |
title_full |
Minimal Enzymes Cocktail Development by Filamentous Fungi Consortia in Solid-State Cultivation and Valorization of Pineapple Crown Waste by Enzymatic Saccharification |
title_fullStr |
Minimal Enzymes Cocktail Development by Filamentous Fungi Consortia in Solid-State Cultivation and Valorization of Pineapple Crown Waste by Enzymatic Saccharification |
title_full_unstemmed |
Minimal Enzymes Cocktail Development by Filamentous Fungi Consortia in Solid-State Cultivation and Valorization of Pineapple Crown Waste by Enzymatic Saccharification |
title_sort |
Minimal Enzymes Cocktail Development by Filamentous Fungi Consortia in Solid-State Cultivation and Valorization of Pineapple Crown Waste by Enzymatic Saccharification |
author |
Teixeira, Wéllen Fabrícia Aviz |
author_facet |
Teixeira, Wéllen Fabrícia Aviz Batista, Ryhára Dias do Amaral Santos, Claudia Cristina Auler Júnior, Aloísio Chagas Freitas Terrasan, César Rafael Fanchini de Santana, Marcio Waluce Pinheiro R. de Siqueira, Félix Gonçalves de Paula-Elias, Fabrício Coutinho de Almeida, Alex Fernando |
author_role |
author |
author2 |
Batista, Ryhára Dias do Amaral Santos, Claudia Cristina Auler Júnior, Aloísio Chagas Freitas Terrasan, César Rafael Fanchini de Santana, Marcio Waluce Pinheiro R. de Siqueira, Félix Gonçalves de Paula-Elias, Fabrício Coutinho de Almeida, Alex Fernando |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Federal University of Tocantins Univ. Est. Paulista Universidade Federal da Bahia (UFBA) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Teixeira, Wéllen Fabrícia Aviz Batista, Ryhára Dias do Amaral Santos, Claudia Cristina Auler Júnior, Aloísio Chagas Freitas Terrasan, César Rafael Fanchini de Santana, Marcio Waluce Pinheiro R. de Siqueira, Félix Gonçalves de Paula-Elias, Fabrício Coutinho de Almeida, Alex Fernando |
dc.subject.por.fl_str_mv |
biomass saccharification Enzyme production Microbial consortia Solid-state cultivation Trichoderma |
topic |
biomass saccharification Enzyme production Microbial consortia Solid-state cultivation Trichoderma |
description |
Agroindustrial wastes is an interesting opportunity for enzymes production, reducing operational costs and strengthening the biorefineries concept. The conversion of lignocellulosic waste to fermentable sugars is current challenge for biorefineries industries. Enzyme cocktail used in delignification and saccharification platform are produced by a combinatory of several lignocellulolytic enzymes. The aim of this study was to develop filamentous fungi compatible-consortia isolated from pineapple wastes for cellulose-degrading enzymes production using pineapple crown waste in solid-state cultivation and its potential for saccharification of pineapple crown waste. Isolates were screened in agar-plate and in solid-state cultivation for cellulases, xylanase and pectinases productions. Six compatible consortia of Trichoderma strains with Aspergillus niger or Pleurotus ostreatus were evaluated for SSC using pineapple crown. All consortia increased the enzymes production compared to monoculture. Consortia of Trichoderma asperellum PEC-6 and P. ostreatus increased 1.60-fold the xylanase and 1.42-fold the β-glucosidase productions. T. asperellum PEC-17 and A. niger increased 1.16-fold endoglucanase and 1.28-fold pectinase. Saccharification of pineapple crown waste using enzyme cocktail produced by filamentous fungi consortia increased the total reducing sugar released in 12.50–13.93% compared to enzymes produced by monocultures. This study provides an alternative model to the cocktail enzyme production by mixed cultures development with lower cost of on-site enzyme manufacture by use of agroindustrial wastes eliminating an enzyme blended step. Moreover, the procedure used in this work can be potentially cost saving and environmentally friendly and should be explored on other bioenergy feedstocks and feed production. Graphic Abstract: [Figure not available: see fulltext.] |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-05-01 2022-04-28T19:29:15Z 2022-04-28T19:29:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s12649-020-01199-8 Waste and Biomass Valorization, v. 12, n. 5, p. 2521-2539, 2021. 1877-265X 1877-2641 http://hdl.handle.net/11449/221532 10.1007/s12649-020-01199-8 2-s2.0-85089476777 |
url |
http://dx.doi.org/10.1007/s12649-020-01199-8 http://hdl.handle.net/11449/221532 |
identifier_str_mv |
Waste and Biomass Valorization, v. 12, n. 5, p. 2521-2539, 2021. 1877-265X 1877-2641 10.1007/s12649-020-01199-8 2-s2.0-85089476777 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Waste and Biomass Valorization |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2521-2539 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964882767970304 |