Evidence of bottleneck effect on hepatitis C virus transmission between a couple under interferon based therapy

Detalhes bibliográficos
Autor(a) principal: da Silva, Rafael Alves
Data de Publicação: 2017
Outros Autores: de Carvalho, Isabel Maria Vicente Guedes, de Matos, Renata Prandini Adum [UNESP], Yamasaki, Lilian Hiromi Tomonari [UNESP], Bittar, Cíntia [UNESP], Rahal, Paula [UNESP], Jardim, Ana Carolina Gomes [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.meegid.2016.11.012
http://hdl.handle.net/11449/178439
Resumo: Issues on the correlation of viral genetic diversity and treatment response to the hepatitis C infection remain uncertain. The bottleneck effect dictates the characteristics of the viral population that will establish the infection in a new host and is related to how the immune system and treatment will be effective against the virus. Here we evaluated the phylogenetic characteristics of quasispecies population and the treatment response pattern of a HCV infected couple. We also analyzed whether the viral population of these patients indicated that they were exposed to the same source for primer infection. This study included two patients (P10 and P11) HCV genotype 1b infected. The couple presented horizontal transmission. Viral RNA was isolated from serum samples collected before, during and after treatment, at specific time points. The HCV NS5A gene sequence was amplified, cloned and sequenced. Genetic and evolutionary analyses were performed to compare the quasispecies population of these two patients and local control patients. Genetic distance and diversity were calculated. Phylogenetic analyses were performed by using maximum likelihood and Bayesian methodologies. The analysis of the baseline samples showed that the genetic distance of the viral populations of patients P10 and P11 was significantly lower than when these patients and the control group based on sequences from local patients were analyzed, supporting the horizontal transmission hypothesis. Phylogenetic analysis with sequences from all the time point samples also demonstrated two patterns of evolution depending on the treatment response. The Bayesian analysis showed that one isolate corresponding to the baseline sample of P10 was grouped into the P11 clade, suggesting a way of infection and a bottleneck effect. Our data suggests that the patient P11 viral population may be originated from variants from P10 patient and consequently showing that clinical differences between treatment responses can emerge from the bottleneck effect on viral populations.
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spelling Evidence of bottleneck effect on hepatitis C virus transmission between a couple under interferon based therapyBottleneckHepatitis CPhylogeniesViral evolutionViral transmissionIssues on the correlation of viral genetic diversity and treatment response to the hepatitis C infection remain uncertain. The bottleneck effect dictates the characteristics of the viral population that will establish the infection in a new host and is related to how the immune system and treatment will be effective against the virus. Here we evaluated the phylogenetic characteristics of quasispecies population and the treatment response pattern of a HCV infected couple. We also analyzed whether the viral population of these patients indicated that they were exposed to the same source for primer infection. This study included two patients (P10 and P11) HCV genotype 1b infected. The couple presented horizontal transmission. Viral RNA was isolated from serum samples collected before, during and after treatment, at specific time points. The HCV NS5A gene sequence was amplified, cloned and sequenced. Genetic and evolutionary analyses were performed to compare the quasispecies population of these two patients and local control patients. Genetic distance and diversity were calculated. Phylogenetic analyses were performed by using maximum likelihood and Bayesian methodologies. The analysis of the baseline samples showed that the genetic distance of the viral populations of patients P10 and P11 was significantly lower than when these patients and the control group based on sequences from local patients were analyzed, supporting the horizontal transmission hypothesis. Phylogenetic analysis with sequences from all the time point samples also demonstrated two patterns of evolution depending on the treatment response. The Bayesian analysis showed that one isolate corresponding to the baseline sample of P10 was grouped into the P11 clade, suggesting a way of infection and a bottleneck effect. Our data suggests that the patient P11 viral population may be originated from variants from P10 patient and consequently showing that clinical differences between treatment responses can emerge from the bottleneck effect on viral populations.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratório de Hepatologia Molecular Aplicada LHEMA Disciplina de Gastroenterologia Departamento de Medicina Universidade Federal de São Paulo, Av. Pedro de Toledo n° 669, 5° AndarLaboratório de Parasitologia Instituto Butantan, Av. Vital Brazil, n° 1500Laboratório de Estudos Genômicos Ibilce UNESPLaboratório de Virologia Instituto de Ciências Biomédicas Universidade Federal de Uberlândia — UFULaboratório de Estudos Genômicos Ibilce UNESPFAPESP: 2007/52073-0FAPESP: 2008/51165-1FAPESP: 2010/06156-4FAPESP: 2012/18168-2Universidade Federal de São Paulo (UNIFESP)Instituto ButantanUniversidade Estadual Paulista (Unesp)Universidade Federal de Uberlândia (UFU)da Silva, Rafael Alvesde Carvalho, Isabel Maria Vicente Guedesde Matos, Renata Prandini Adum [UNESP]Yamasaki, Lilian Hiromi Tomonari [UNESP]Bittar, Cíntia [UNESP]Rahal, Paula [UNESP]Jardim, Ana Carolina Gomes [UNESP]2018-12-11T17:30:21Z2018-12-11T17:30:21Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article87-93application/pdfhttp://dx.doi.org/10.1016/j.meegid.2016.11.012Infection, Genetics and Evolution, v. 47, p. 87-93.1567-72571567-1348http://hdl.handle.net/11449/17843910.1016/j.meegid.2016.11.0122-s2.0-849978319532-s2.0-84997831953.pdf79910823626712120000-0001-5693-6148Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInfection, Genetics and Evolution1,278info:eu-repo/semantics/openAccess2023-12-12T06:18:13Zoai:repositorio.unesp.br:11449/178439Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:06:48.417518Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Evidence of bottleneck effect on hepatitis C virus transmission between a couple under interferon based therapy
title Evidence of bottleneck effect on hepatitis C virus transmission between a couple under interferon based therapy
spellingShingle Evidence of bottleneck effect on hepatitis C virus transmission between a couple under interferon based therapy
da Silva, Rafael Alves
Bottleneck
Hepatitis C
Phylogenies
Viral evolution
Viral transmission
title_short Evidence of bottleneck effect on hepatitis C virus transmission between a couple under interferon based therapy
title_full Evidence of bottleneck effect on hepatitis C virus transmission between a couple under interferon based therapy
title_fullStr Evidence of bottleneck effect on hepatitis C virus transmission between a couple under interferon based therapy
title_full_unstemmed Evidence of bottleneck effect on hepatitis C virus transmission between a couple under interferon based therapy
title_sort Evidence of bottleneck effect on hepatitis C virus transmission between a couple under interferon based therapy
author da Silva, Rafael Alves
author_facet da Silva, Rafael Alves
de Carvalho, Isabel Maria Vicente Guedes
de Matos, Renata Prandini Adum [UNESP]
Yamasaki, Lilian Hiromi Tomonari [UNESP]
Bittar, Cíntia [UNESP]
Rahal, Paula [UNESP]
Jardim, Ana Carolina Gomes [UNESP]
author_role author
author2 de Carvalho, Isabel Maria Vicente Guedes
de Matos, Renata Prandini Adum [UNESP]
Yamasaki, Lilian Hiromi Tomonari [UNESP]
Bittar, Cíntia [UNESP]
Rahal, Paula [UNESP]
Jardim, Ana Carolina Gomes [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Instituto Butantan
Universidade Estadual Paulista (Unesp)
Universidade Federal de Uberlândia (UFU)
dc.contributor.author.fl_str_mv da Silva, Rafael Alves
de Carvalho, Isabel Maria Vicente Guedes
de Matos, Renata Prandini Adum [UNESP]
Yamasaki, Lilian Hiromi Tomonari [UNESP]
Bittar, Cíntia [UNESP]
Rahal, Paula [UNESP]
Jardim, Ana Carolina Gomes [UNESP]
dc.subject.por.fl_str_mv Bottleneck
Hepatitis C
Phylogenies
Viral evolution
Viral transmission
topic Bottleneck
Hepatitis C
Phylogenies
Viral evolution
Viral transmission
description Issues on the correlation of viral genetic diversity and treatment response to the hepatitis C infection remain uncertain. The bottleneck effect dictates the characteristics of the viral population that will establish the infection in a new host and is related to how the immune system and treatment will be effective against the virus. Here we evaluated the phylogenetic characteristics of quasispecies population and the treatment response pattern of a HCV infected couple. We also analyzed whether the viral population of these patients indicated that they were exposed to the same source for primer infection. This study included two patients (P10 and P11) HCV genotype 1b infected. The couple presented horizontal transmission. Viral RNA was isolated from serum samples collected before, during and after treatment, at specific time points. The HCV NS5A gene sequence was amplified, cloned and sequenced. Genetic and evolutionary analyses were performed to compare the quasispecies population of these two patients and local control patients. Genetic distance and diversity were calculated. Phylogenetic analyses were performed by using maximum likelihood and Bayesian methodologies. The analysis of the baseline samples showed that the genetic distance of the viral populations of patients P10 and P11 was significantly lower than when these patients and the control group based on sequences from local patients were analyzed, supporting the horizontal transmission hypothesis. Phylogenetic analysis with sequences from all the time point samples also demonstrated two patterns of evolution depending on the treatment response. The Bayesian analysis showed that one isolate corresponding to the baseline sample of P10 was grouped into the P11 clade, suggesting a way of infection and a bottleneck effect. Our data suggests that the patient P11 viral population may be originated from variants from P10 patient and consequently showing that clinical differences between treatment responses can emerge from the bottleneck effect on viral populations.
publishDate 2017
dc.date.none.fl_str_mv 2017-01-01
2018-12-11T17:30:21Z
2018-12-11T17:30:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.meegid.2016.11.012
Infection, Genetics and Evolution, v. 47, p. 87-93.
1567-7257
1567-1348
http://hdl.handle.net/11449/178439
10.1016/j.meegid.2016.11.012
2-s2.0-84997831953
2-s2.0-84997831953.pdf
7991082362671212
0000-0001-5693-6148
url http://dx.doi.org/10.1016/j.meegid.2016.11.012
http://hdl.handle.net/11449/178439
identifier_str_mv Infection, Genetics and Evolution, v. 47, p. 87-93.
1567-7257
1567-1348
10.1016/j.meegid.2016.11.012
2-s2.0-84997831953
2-s2.0-84997831953.pdf
7991082362671212
0000-0001-5693-6148
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Infection, Genetics and Evolution
1,278
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 87-93
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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