Evidence of bottleneck effect on hepatitis C virus transmission between a couple under interferon based therapy
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.meegid.2016.11.012 http://hdl.handle.net/11449/178439 |
Resumo: | Issues on the correlation of viral genetic diversity and treatment response to the hepatitis C infection remain uncertain. The bottleneck effect dictates the characteristics of the viral population that will establish the infection in a new host and is related to how the immune system and treatment will be effective against the virus. Here we evaluated the phylogenetic characteristics of quasispecies population and the treatment response pattern of a HCV infected couple. We also analyzed whether the viral population of these patients indicated that they were exposed to the same source for primer infection. This study included two patients (P10 and P11) HCV genotype 1b infected. The couple presented horizontal transmission. Viral RNA was isolated from serum samples collected before, during and after treatment, at specific time points. The HCV NS5A gene sequence was amplified, cloned and sequenced. Genetic and evolutionary analyses were performed to compare the quasispecies population of these two patients and local control patients. Genetic distance and diversity were calculated. Phylogenetic analyses were performed by using maximum likelihood and Bayesian methodologies. The analysis of the baseline samples showed that the genetic distance of the viral populations of patients P10 and P11 was significantly lower than when these patients and the control group based on sequences from local patients were analyzed, supporting the horizontal transmission hypothesis. Phylogenetic analysis with sequences from all the time point samples also demonstrated two patterns of evolution depending on the treatment response. The Bayesian analysis showed that one isolate corresponding to the baseline sample of P10 was grouped into the P11 clade, suggesting a way of infection and a bottleneck effect. Our data suggests that the patient P11 viral population may be originated from variants from P10 patient and consequently showing that clinical differences between treatment responses can emerge from the bottleneck effect on viral populations. |
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Evidence of bottleneck effect on hepatitis C virus transmission between a couple under interferon based therapyBottleneckHepatitis CPhylogeniesViral evolutionViral transmissionIssues on the correlation of viral genetic diversity and treatment response to the hepatitis C infection remain uncertain. The bottleneck effect dictates the characteristics of the viral population that will establish the infection in a new host and is related to how the immune system and treatment will be effective against the virus. Here we evaluated the phylogenetic characteristics of quasispecies population and the treatment response pattern of a HCV infected couple. We also analyzed whether the viral population of these patients indicated that they were exposed to the same source for primer infection. This study included two patients (P10 and P11) HCV genotype 1b infected. The couple presented horizontal transmission. Viral RNA was isolated from serum samples collected before, during and after treatment, at specific time points. The HCV NS5A gene sequence was amplified, cloned and sequenced. Genetic and evolutionary analyses were performed to compare the quasispecies population of these two patients and local control patients. Genetic distance and diversity were calculated. Phylogenetic analyses were performed by using maximum likelihood and Bayesian methodologies. The analysis of the baseline samples showed that the genetic distance of the viral populations of patients P10 and P11 was significantly lower than when these patients and the control group based on sequences from local patients were analyzed, supporting the horizontal transmission hypothesis. Phylogenetic analysis with sequences from all the time point samples also demonstrated two patterns of evolution depending on the treatment response. The Bayesian analysis showed that one isolate corresponding to the baseline sample of P10 was grouped into the P11 clade, suggesting a way of infection and a bottleneck effect. Our data suggests that the patient P11 viral population may be originated from variants from P10 patient and consequently showing that clinical differences between treatment responses can emerge from the bottleneck effect on viral populations.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratório de Hepatologia Molecular Aplicada LHEMA Disciplina de Gastroenterologia Departamento de Medicina Universidade Federal de São Paulo, Av. Pedro de Toledo n° 669, 5° AndarLaboratório de Parasitologia Instituto Butantan, Av. Vital Brazil, n° 1500Laboratório de Estudos Genômicos Ibilce UNESPLaboratório de Virologia Instituto de Ciências Biomédicas Universidade Federal de Uberlândia — UFULaboratório de Estudos Genômicos Ibilce UNESPFAPESP: 2007/52073-0FAPESP: 2008/51165-1FAPESP: 2010/06156-4FAPESP: 2012/18168-2Universidade Federal de São Paulo (UNIFESP)Instituto ButantanUniversidade Estadual Paulista (Unesp)Universidade Federal de Uberlândia (UFU)da Silva, Rafael Alvesde Carvalho, Isabel Maria Vicente Guedesde Matos, Renata Prandini Adum [UNESP]Yamasaki, Lilian Hiromi Tomonari [UNESP]Bittar, Cíntia [UNESP]Rahal, Paula [UNESP]Jardim, Ana Carolina Gomes [UNESP]2018-12-11T17:30:21Z2018-12-11T17:30:21Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article87-93application/pdfhttp://dx.doi.org/10.1016/j.meegid.2016.11.012Infection, Genetics and Evolution, v. 47, p. 87-93.1567-72571567-1348http://hdl.handle.net/11449/17843910.1016/j.meegid.2016.11.0122-s2.0-849978319532-s2.0-84997831953.pdf79910823626712120000-0001-5693-6148Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInfection, Genetics and Evolution1,278info:eu-repo/semantics/openAccess2023-12-12T06:18:13Zoai:repositorio.unesp.br:11449/178439Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:06:48.417518Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evidence of bottleneck effect on hepatitis C virus transmission between a couple under interferon based therapy |
title |
Evidence of bottleneck effect on hepatitis C virus transmission between a couple under interferon based therapy |
spellingShingle |
Evidence of bottleneck effect on hepatitis C virus transmission between a couple under interferon based therapy da Silva, Rafael Alves Bottleneck Hepatitis C Phylogenies Viral evolution Viral transmission |
title_short |
Evidence of bottleneck effect on hepatitis C virus transmission between a couple under interferon based therapy |
title_full |
Evidence of bottleneck effect on hepatitis C virus transmission between a couple under interferon based therapy |
title_fullStr |
Evidence of bottleneck effect on hepatitis C virus transmission between a couple under interferon based therapy |
title_full_unstemmed |
Evidence of bottleneck effect on hepatitis C virus transmission between a couple under interferon based therapy |
title_sort |
Evidence of bottleneck effect on hepatitis C virus transmission between a couple under interferon based therapy |
author |
da Silva, Rafael Alves |
author_facet |
da Silva, Rafael Alves de Carvalho, Isabel Maria Vicente Guedes de Matos, Renata Prandini Adum [UNESP] Yamasaki, Lilian Hiromi Tomonari [UNESP] Bittar, Cíntia [UNESP] Rahal, Paula [UNESP] Jardim, Ana Carolina Gomes [UNESP] |
author_role |
author |
author2 |
de Carvalho, Isabel Maria Vicente Guedes de Matos, Renata Prandini Adum [UNESP] Yamasaki, Lilian Hiromi Tomonari [UNESP] Bittar, Cíntia [UNESP] Rahal, Paula [UNESP] Jardim, Ana Carolina Gomes [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Instituto Butantan Universidade Estadual Paulista (Unesp) Universidade Federal de Uberlândia (UFU) |
dc.contributor.author.fl_str_mv |
da Silva, Rafael Alves de Carvalho, Isabel Maria Vicente Guedes de Matos, Renata Prandini Adum [UNESP] Yamasaki, Lilian Hiromi Tomonari [UNESP] Bittar, Cíntia [UNESP] Rahal, Paula [UNESP] Jardim, Ana Carolina Gomes [UNESP] |
dc.subject.por.fl_str_mv |
Bottleneck Hepatitis C Phylogenies Viral evolution Viral transmission |
topic |
Bottleneck Hepatitis C Phylogenies Viral evolution Viral transmission |
description |
Issues on the correlation of viral genetic diversity and treatment response to the hepatitis C infection remain uncertain. The bottleneck effect dictates the characteristics of the viral population that will establish the infection in a new host and is related to how the immune system and treatment will be effective against the virus. Here we evaluated the phylogenetic characteristics of quasispecies population and the treatment response pattern of a HCV infected couple. We also analyzed whether the viral population of these patients indicated that they were exposed to the same source for primer infection. This study included two patients (P10 and P11) HCV genotype 1b infected. The couple presented horizontal transmission. Viral RNA was isolated from serum samples collected before, during and after treatment, at specific time points. The HCV NS5A gene sequence was amplified, cloned and sequenced. Genetic and evolutionary analyses were performed to compare the quasispecies population of these two patients and local control patients. Genetic distance and diversity were calculated. Phylogenetic analyses were performed by using maximum likelihood and Bayesian methodologies. The analysis of the baseline samples showed that the genetic distance of the viral populations of patients P10 and P11 was significantly lower than when these patients and the control group based on sequences from local patients were analyzed, supporting the horizontal transmission hypothesis. Phylogenetic analysis with sequences from all the time point samples also demonstrated two patterns of evolution depending on the treatment response. The Bayesian analysis showed that one isolate corresponding to the baseline sample of P10 was grouped into the P11 clade, suggesting a way of infection and a bottleneck effect. Our data suggests that the patient P11 viral population may be originated from variants from P10 patient and consequently showing that clinical differences between treatment responses can emerge from the bottleneck effect on viral populations. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 2018-12-11T17:30:21Z 2018-12-11T17:30:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.meegid.2016.11.012 Infection, Genetics and Evolution, v. 47, p. 87-93. 1567-7257 1567-1348 http://hdl.handle.net/11449/178439 10.1016/j.meegid.2016.11.012 2-s2.0-84997831953 2-s2.0-84997831953.pdf 7991082362671212 0000-0001-5693-6148 |
url |
http://dx.doi.org/10.1016/j.meegid.2016.11.012 http://hdl.handle.net/11449/178439 |
identifier_str_mv |
Infection, Genetics and Evolution, v. 47, p. 87-93. 1567-7257 1567-1348 10.1016/j.meegid.2016.11.012 2-s2.0-84997831953 2-s2.0-84997831953.pdf 7991082362671212 0000-0001-5693-6148 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Infection, Genetics and Evolution 1,278 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
87-93 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129161573695488 |