Apoptosis-related gene expression induced by Colombian propolis samples in canine osteosarcoma cell line
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.14202/vetworld.2021.964-971 http://hdl.handle.net/11449/208646 |
Resumo: | Background and Aim: Osteosarcoma (OSA) is the most common bone tumor in canines and humans. This study aimed to assess the cytotoxic and apoptotic effects of Colombian propolis samples on a canine OSA cell line (OSCA-8) by evaluating the expression of BCL-2, BAX, CASPASE 9, CASPASE 8, and TNFR1 genes involved in the apoptosis pathway. Materials and Methods: After treating the cells with five Colombian propolis samples (Usm, Met, Fus, Sil, and Caj), we evaluated cell viability and lactate dehydrogenase (LDH) release. Early and late apoptosis was determined by flow cytometry using annexin V/propidium iodide. Furthermore, the effects of three selected samples on gene expression were analyzed by real-time polymerase chain reaction. Results: The Colombian propolis samples reduced OSCA-8 cell viability and increased LDH release. All samples induced apoptosis significantly and upregulated BCL-2 and CASPASE 8 expression. Usm and Sil increased BAX expression, Met and Sil induced CASPASE 9 expression, and Usm increased TNFR1. Conclusion: Colombian propolis samples exhibited cytotoxic and apoptotic effects on canine OSA cells, and CASPASE 8 upregulation indicated apoptosis induction by the extrinsic pathway. |
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Repositório Institucional da UNESP |
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spelling |
Apoptosis-related gene expression induced by Colombian propolis samples in canine osteosarcoma cell lineApoptosisCytotoxicityOsteosarcomaPropolisBackground and Aim: Osteosarcoma (OSA) is the most common bone tumor in canines and humans. This study aimed to assess the cytotoxic and apoptotic effects of Colombian propolis samples on a canine OSA cell line (OSCA-8) by evaluating the expression of BCL-2, BAX, CASPASE 9, CASPASE 8, and TNFR1 genes involved in the apoptosis pathway. Materials and Methods: After treating the cells with five Colombian propolis samples (Usm, Met, Fus, Sil, and Caj), we evaluated cell viability and lactate dehydrogenase (LDH) release. Early and late apoptosis was determined by flow cytometry using annexin V/propidium iodide. Furthermore, the effects of three selected samples on gene expression were analyzed by real-time polymerase chain reaction. Results: The Colombian propolis samples reduced OSCA-8 cell viability and increased LDH release. All samples induced apoptosis significantly and upregulated BCL-2 and CASPASE 8 expression. Usm and Sil increased BAX expression, Met and Sil induced CASPASE 9 expression, and Usm increased TNFR1. Conclusion: Colombian propolis samples exhibited cytotoxic and apoptotic effects on canine OSA cells, and CASPASE 8 upregulation indicated apoptosis induction by the extrinsic pathway.Department of Animal Health Facultad de Medicina Veterinaria Universidad Antonio NariñoDepartment of Morphology Facultad de Medicina Universidad Nacional de ColombiaDepartment of Chemical and Biological Sciences São Paulo State University (UNESP) Institute of BiosciencesDepartment of Chemical and Biological Sciences São Paulo State University (UNESP) Institute of BiosciencesUniversidad Antonio NariñoUniversidad Nacional de ColombiaUniversidade Estadual Paulista (Unesp)Pardo-Mora, Dolly PatriciaMurillo, Oscar JuliánRey-Buitrago, MauricioLosada-Barragán, MónicaUribe, Jaime Fabian CruzSantiago, Karina Basso [UNESP]Conti, Bruno José [UNESP]de Oliveira Cardoso, Eliza [UNESP]Conte, Fernanda Lopes [UNESP]Gutiérrez, Rafael MaríaGarcía, Orlando TorresSforcin, José Maurício [UNESP]2021-06-25T11:15:36Z2021-06-25T11:15:36Z2021-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article964-971http://dx.doi.org/10.14202/vetworld.2021.964-971Veterinary World, v. 14, n. 4, p. 964-971, 2021.2231-09160972-8988http://hdl.handle.net/11449/20864610.14202/vetworld.2021.964-9712-s2.0-85105266911Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Worldinfo:eu-repo/semantics/openAccess2021-10-23T19:02:22Zoai:repositorio.unesp.br:11449/208646Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:26:35.410647Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Apoptosis-related gene expression induced by Colombian propolis samples in canine osteosarcoma cell line |
title |
Apoptosis-related gene expression induced by Colombian propolis samples in canine osteosarcoma cell line |
spellingShingle |
Apoptosis-related gene expression induced by Colombian propolis samples in canine osteosarcoma cell line Pardo-Mora, Dolly Patricia Apoptosis Cytotoxicity Osteosarcoma Propolis |
title_short |
Apoptosis-related gene expression induced by Colombian propolis samples in canine osteosarcoma cell line |
title_full |
Apoptosis-related gene expression induced by Colombian propolis samples in canine osteosarcoma cell line |
title_fullStr |
Apoptosis-related gene expression induced by Colombian propolis samples in canine osteosarcoma cell line |
title_full_unstemmed |
Apoptosis-related gene expression induced by Colombian propolis samples in canine osteosarcoma cell line |
title_sort |
Apoptosis-related gene expression induced by Colombian propolis samples in canine osteosarcoma cell line |
author |
Pardo-Mora, Dolly Patricia |
author_facet |
Pardo-Mora, Dolly Patricia Murillo, Oscar Julián Rey-Buitrago, Mauricio Losada-Barragán, Mónica Uribe, Jaime Fabian Cruz Santiago, Karina Basso [UNESP] Conti, Bruno José [UNESP] de Oliveira Cardoso, Eliza [UNESP] Conte, Fernanda Lopes [UNESP] Gutiérrez, Rafael María García, Orlando Torres Sforcin, José Maurício [UNESP] |
author_role |
author |
author2 |
Murillo, Oscar Julián Rey-Buitrago, Mauricio Losada-Barragán, Mónica Uribe, Jaime Fabian Cruz Santiago, Karina Basso [UNESP] Conti, Bruno José [UNESP] de Oliveira Cardoso, Eliza [UNESP] Conte, Fernanda Lopes [UNESP] Gutiérrez, Rafael María García, Orlando Torres Sforcin, José Maurício [UNESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidad Antonio Nariño Universidad Nacional de Colombia Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Pardo-Mora, Dolly Patricia Murillo, Oscar Julián Rey-Buitrago, Mauricio Losada-Barragán, Mónica Uribe, Jaime Fabian Cruz Santiago, Karina Basso [UNESP] Conti, Bruno José [UNESP] de Oliveira Cardoso, Eliza [UNESP] Conte, Fernanda Lopes [UNESP] Gutiérrez, Rafael María García, Orlando Torres Sforcin, José Maurício [UNESP] |
dc.subject.por.fl_str_mv |
Apoptosis Cytotoxicity Osteosarcoma Propolis |
topic |
Apoptosis Cytotoxicity Osteosarcoma Propolis |
description |
Background and Aim: Osteosarcoma (OSA) is the most common bone tumor in canines and humans. This study aimed to assess the cytotoxic and apoptotic effects of Colombian propolis samples on a canine OSA cell line (OSCA-8) by evaluating the expression of BCL-2, BAX, CASPASE 9, CASPASE 8, and TNFR1 genes involved in the apoptosis pathway. Materials and Methods: After treating the cells with five Colombian propolis samples (Usm, Met, Fus, Sil, and Caj), we evaluated cell viability and lactate dehydrogenase (LDH) release. Early and late apoptosis was determined by flow cytometry using annexin V/propidium iodide. Furthermore, the effects of three selected samples on gene expression were analyzed by real-time polymerase chain reaction. Results: The Colombian propolis samples reduced OSCA-8 cell viability and increased LDH release. All samples induced apoptosis significantly and upregulated BCL-2 and CASPASE 8 expression. Usm and Sil increased BAX expression, Met and Sil induced CASPASE 9 expression, and Usm increased TNFR1. Conclusion: Colombian propolis samples exhibited cytotoxic and apoptotic effects on canine OSA cells, and CASPASE 8 upregulation indicated apoptosis induction by the extrinsic pathway. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:15:36Z 2021-06-25T11:15:36Z 2021-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.14202/vetworld.2021.964-971 Veterinary World, v. 14, n. 4, p. 964-971, 2021. 2231-0916 0972-8988 http://hdl.handle.net/11449/208646 10.14202/vetworld.2021.964-971 2-s2.0-85105266911 |
url |
http://dx.doi.org/10.14202/vetworld.2021.964-971 http://hdl.handle.net/11449/208646 |
identifier_str_mv |
Veterinary World, v. 14, n. 4, p. 964-971, 2021. 2231-0916 0972-8988 10.14202/vetworld.2021.964-971 2-s2.0-85105266911 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Veterinary World |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
964-971 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128360521400320 |