Microarray analysis of canine osteosarcoma cells exposed to Colombian propolis

Detalhes bibliográficos
Autor(a) principal: Pardo-Mora, Dolly Patricia
Data de Publicação: 2022
Outros Autores: Murillo, Oscar Julián, Buitrago, Mauricio Rey, Rodríguez, Anny, Uribe, Jaime Fabian Cruz, Sforcin, José Maurício [UNESP], García, Orlando Torres
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.phyplu.2022.100356
http://hdl.handle.net/11449/249233
Resumo: Background: Propolis is a bee product exhibiting a cytotoxic activity against tumor cells. This study aimed at investigating the gene expression profile of canine osteosarcoma cells exposed to Colombian propolis by microarray analysis, in an attempt to identify differentially expressed genes and the pathways involved in propolis cytotoxic action. Methods: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase assays were used to assess the viability and the half-maximal inhibitory concentration for OSCA-8 cells and fibroblasts after incubation with propolis samples, its solvent (70% ethanol) or doxorubicin (positive control). The gene expression profile was determined by the Canine Gene 1.0 ST Array (Affymetrix) and the microarray data was validated by real time PCR. Enrichment and annotation analysis were applied for the differentially expressed genes. Results: Propolis reduced cell viability and exerted a cytotoxic effect on OSCA-8 cells, exhibiting a selective effect in relation to non-tumor cells. The microarray analysis showed changes in more than 253 genes involved in intracellular pathways related to the inflammatory response, growth factor activity and chemotaxis, which are associated with cancer development. Propolis exerted doxorubicin-like changes in gene expression. Conclusion: Propolis and Dox exerted a similar action, altering some pathways and indicating possible mechanisms of action involved in the cytotoxic effect on OSCA-8 cells.
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spelling Microarray analysis of canine osteosarcoma cells exposed to Colombian propolisGene expressionMicroarray analysisOsteosarcomaPropolisBackground: Propolis is a bee product exhibiting a cytotoxic activity against tumor cells. This study aimed at investigating the gene expression profile of canine osteosarcoma cells exposed to Colombian propolis by microarray analysis, in an attempt to identify differentially expressed genes and the pathways involved in propolis cytotoxic action. Methods: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase assays were used to assess the viability and the half-maximal inhibitory concentration for OSCA-8 cells and fibroblasts after incubation with propolis samples, its solvent (70% ethanol) or doxorubicin (positive control). The gene expression profile was determined by the Canine Gene 1.0 ST Array (Affymetrix) and the microarray data was validated by real time PCR. Enrichment and annotation analysis were applied for the differentially expressed genes. Results: Propolis reduced cell viability and exerted a cytotoxic effect on OSCA-8 cells, exhibiting a selective effect in relation to non-tumor cells. The microarray analysis showed changes in more than 253 genes involved in intracellular pathways related to the inflammatory response, growth factor activity and chemotaxis, which are associated with cancer development. Propolis exerted doxorubicin-like changes in gene expression. Conclusion: Propolis and Dox exerted a similar action, altering some pathways and indicating possible mechanisms of action involved in the cytotoxic effect on OSCA-8 cells.Faculty of Medicine Veterinary and Faculty of Sciences Antonio Nariño UniversityFaculty of Medicine Universidad Nacional de ColombiaSão Paulo State University (UNESP) Institute of Biosciences, Campus BotucatuSão Paulo State University (UNESP) Institute of Biosciences, Campus BotucatuAntonio Nariño UniversityUniversidad Nacional de ColombiaUniversidade Estadual Paulista (UNESP)Pardo-Mora, Dolly PatriciaMurillo, Oscar JuliánBuitrago, Mauricio ReyRodríguez, AnnyUribe, Jaime Fabian CruzSforcin, José Maurício [UNESP]García, Orlando Torres2023-07-29T14:32:27Z2023-07-29T14:32:27Z2022-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.phyplu.2022.100356Phytomedicine Plus, v. 2, n. 4, 2022.2667-0313http://hdl.handle.net/11449/24923310.1016/j.phyplu.2022.1003562-s2.0-85139360753Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhytomedicine Plusinfo:eu-repo/semantics/openAccess2023-07-29T14:32:27Zoai:repositorio.unesp.br:11449/249233Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:58:16.578311Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Microarray analysis of canine osteosarcoma cells exposed to Colombian propolis
title Microarray analysis of canine osteosarcoma cells exposed to Colombian propolis
spellingShingle Microarray analysis of canine osteosarcoma cells exposed to Colombian propolis
Pardo-Mora, Dolly Patricia
Gene expression
Microarray analysis
Osteosarcoma
Propolis
title_short Microarray analysis of canine osteosarcoma cells exposed to Colombian propolis
title_full Microarray analysis of canine osteosarcoma cells exposed to Colombian propolis
title_fullStr Microarray analysis of canine osteosarcoma cells exposed to Colombian propolis
title_full_unstemmed Microarray analysis of canine osteosarcoma cells exposed to Colombian propolis
title_sort Microarray analysis of canine osteosarcoma cells exposed to Colombian propolis
author Pardo-Mora, Dolly Patricia
author_facet Pardo-Mora, Dolly Patricia
Murillo, Oscar Julián
Buitrago, Mauricio Rey
Rodríguez, Anny
Uribe, Jaime Fabian Cruz
Sforcin, José Maurício [UNESP]
García, Orlando Torres
author_role author
author2 Murillo, Oscar Julián
Buitrago, Mauricio Rey
Rodríguez, Anny
Uribe, Jaime Fabian Cruz
Sforcin, José Maurício [UNESP]
García, Orlando Torres
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Antonio Nariño University
Universidad Nacional de Colombia
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Pardo-Mora, Dolly Patricia
Murillo, Oscar Julián
Buitrago, Mauricio Rey
Rodríguez, Anny
Uribe, Jaime Fabian Cruz
Sforcin, José Maurício [UNESP]
García, Orlando Torres
dc.subject.por.fl_str_mv Gene expression
Microarray analysis
Osteosarcoma
Propolis
topic Gene expression
Microarray analysis
Osteosarcoma
Propolis
description Background: Propolis is a bee product exhibiting a cytotoxic activity against tumor cells. This study aimed at investigating the gene expression profile of canine osteosarcoma cells exposed to Colombian propolis by microarray analysis, in an attempt to identify differentially expressed genes and the pathways involved in propolis cytotoxic action. Methods: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase assays were used to assess the viability and the half-maximal inhibitory concentration for OSCA-8 cells and fibroblasts after incubation with propolis samples, its solvent (70% ethanol) or doxorubicin (positive control). The gene expression profile was determined by the Canine Gene 1.0 ST Array (Affymetrix) and the microarray data was validated by real time PCR. Enrichment and annotation analysis were applied for the differentially expressed genes. Results: Propolis reduced cell viability and exerted a cytotoxic effect on OSCA-8 cells, exhibiting a selective effect in relation to non-tumor cells. The microarray analysis showed changes in more than 253 genes involved in intracellular pathways related to the inflammatory response, growth factor activity and chemotaxis, which are associated with cancer development. Propolis exerted doxorubicin-like changes in gene expression. Conclusion: Propolis and Dox exerted a similar action, altering some pathways and indicating possible mechanisms of action involved in the cytotoxic effect on OSCA-8 cells.
publishDate 2022
dc.date.none.fl_str_mv 2022-11-01
2023-07-29T14:32:27Z
2023-07-29T14:32:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.phyplu.2022.100356
Phytomedicine Plus, v. 2, n. 4, 2022.
2667-0313
http://hdl.handle.net/11449/249233
10.1016/j.phyplu.2022.100356
2-s2.0-85139360753
url http://dx.doi.org/10.1016/j.phyplu.2022.100356
http://hdl.handle.net/11449/249233
identifier_str_mv Phytomedicine Plus, v. 2, n. 4, 2022.
2667-0313
10.1016/j.phyplu.2022.100356
2-s2.0-85139360753
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Phytomedicine Plus
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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