Microarray analysis of canine osteosarcoma cells exposed to Colombian propolis
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.phyplu.2022.100356 http://hdl.handle.net/11449/249233 |
Resumo: | Background: Propolis is a bee product exhibiting a cytotoxic activity against tumor cells. This study aimed at investigating the gene expression profile of canine osteosarcoma cells exposed to Colombian propolis by microarray analysis, in an attempt to identify differentially expressed genes and the pathways involved in propolis cytotoxic action. Methods: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase assays were used to assess the viability and the half-maximal inhibitory concentration for OSCA-8 cells and fibroblasts after incubation with propolis samples, its solvent (70% ethanol) or doxorubicin (positive control). The gene expression profile was determined by the Canine Gene 1.0 ST Array (Affymetrix) and the microarray data was validated by real time PCR. Enrichment and annotation analysis were applied for the differentially expressed genes. Results: Propolis reduced cell viability and exerted a cytotoxic effect on OSCA-8 cells, exhibiting a selective effect in relation to non-tumor cells. The microarray analysis showed changes in more than 253 genes involved in intracellular pathways related to the inflammatory response, growth factor activity and chemotaxis, which are associated with cancer development. Propolis exerted doxorubicin-like changes in gene expression. Conclusion: Propolis and Dox exerted a similar action, altering some pathways and indicating possible mechanisms of action involved in the cytotoxic effect on OSCA-8 cells. |
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Repositório Institucional da UNESP |
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Microarray analysis of canine osteosarcoma cells exposed to Colombian propolisGene expressionMicroarray analysisOsteosarcomaPropolisBackground: Propolis is a bee product exhibiting a cytotoxic activity against tumor cells. This study aimed at investigating the gene expression profile of canine osteosarcoma cells exposed to Colombian propolis by microarray analysis, in an attempt to identify differentially expressed genes and the pathways involved in propolis cytotoxic action. Methods: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase assays were used to assess the viability and the half-maximal inhibitory concentration for OSCA-8 cells and fibroblasts after incubation with propolis samples, its solvent (70% ethanol) or doxorubicin (positive control). The gene expression profile was determined by the Canine Gene 1.0 ST Array (Affymetrix) and the microarray data was validated by real time PCR. Enrichment and annotation analysis were applied for the differentially expressed genes. Results: Propolis reduced cell viability and exerted a cytotoxic effect on OSCA-8 cells, exhibiting a selective effect in relation to non-tumor cells. The microarray analysis showed changes in more than 253 genes involved in intracellular pathways related to the inflammatory response, growth factor activity and chemotaxis, which are associated with cancer development. Propolis exerted doxorubicin-like changes in gene expression. Conclusion: Propolis and Dox exerted a similar action, altering some pathways and indicating possible mechanisms of action involved in the cytotoxic effect on OSCA-8 cells.Faculty of Medicine Veterinary and Faculty of Sciences Antonio Nariño UniversityFaculty of Medicine Universidad Nacional de ColombiaSão Paulo State University (UNESP) Institute of Biosciences, Campus BotucatuSão Paulo State University (UNESP) Institute of Biosciences, Campus BotucatuAntonio Nariño UniversityUniversidad Nacional de ColombiaUniversidade Estadual Paulista (UNESP)Pardo-Mora, Dolly PatriciaMurillo, Oscar JuliánBuitrago, Mauricio ReyRodríguez, AnnyUribe, Jaime Fabian CruzSforcin, José Maurício [UNESP]García, Orlando Torres2023-07-29T14:32:27Z2023-07-29T14:32:27Z2022-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.phyplu.2022.100356Phytomedicine Plus, v. 2, n. 4, 2022.2667-0313http://hdl.handle.net/11449/24923310.1016/j.phyplu.2022.1003562-s2.0-85139360753Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhytomedicine Plusinfo:eu-repo/semantics/openAccess2023-07-29T14:32:27Zoai:repositorio.unesp.br:11449/249233Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:58:16.578311Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Microarray analysis of canine osteosarcoma cells exposed to Colombian propolis |
title |
Microarray analysis of canine osteosarcoma cells exposed to Colombian propolis |
spellingShingle |
Microarray analysis of canine osteosarcoma cells exposed to Colombian propolis Pardo-Mora, Dolly Patricia Gene expression Microarray analysis Osteosarcoma Propolis |
title_short |
Microarray analysis of canine osteosarcoma cells exposed to Colombian propolis |
title_full |
Microarray analysis of canine osteosarcoma cells exposed to Colombian propolis |
title_fullStr |
Microarray analysis of canine osteosarcoma cells exposed to Colombian propolis |
title_full_unstemmed |
Microarray analysis of canine osteosarcoma cells exposed to Colombian propolis |
title_sort |
Microarray analysis of canine osteosarcoma cells exposed to Colombian propolis |
author |
Pardo-Mora, Dolly Patricia |
author_facet |
Pardo-Mora, Dolly Patricia Murillo, Oscar Julián Buitrago, Mauricio Rey Rodríguez, Anny Uribe, Jaime Fabian Cruz Sforcin, José Maurício [UNESP] García, Orlando Torres |
author_role |
author |
author2 |
Murillo, Oscar Julián Buitrago, Mauricio Rey Rodríguez, Anny Uribe, Jaime Fabian Cruz Sforcin, José Maurício [UNESP] García, Orlando Torres |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Antonio Nariño University Universidad Nacional de Colombia Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Pardo-Mora, Dolly Patricia Murillo, Oscar Julián Buitrago, Mauricio Rey Rodríguez, Anny Uribe, Jaime Fabian Cruz Sforcin, José Maurício [UNESP] García, Orlando Torres |
dc.subject.por.fl_str_mv |
Gene expression Microarray analysis Osteosarcoma Propolis |
topic |
Gene expression Microarray analysis Osteosarcoma Propolis |
description |
Background: Propolis is a bee product exhibiting a cytotoxic activity against tumor cells. This study aimed at investigating the gene expression profile of canine osteosarcoma cells exposed to Colombian propolis by microarray analysis, in an attempt to identify differentially expressed genes and the pathways involved in propolis cytotoxic action. Methods: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase assays were used to assess the viability and the half-maximal inhibitory concentration for OSCA-8 cells and fibroblasts after incubation with propolis samples, its solvent (70% ethanol) or doxorubicin (positive control). The gene expression profile was determined by the Canine Gene 1.0 ST Array (Affymetrix) and the microarray data was validated by real time PCR. Enrichment and annotation analysis were applied for the differentially expressed genes. Results: Propolis reduced cell viability and exerted a cytotoxic effect on OSCA-8 cells, exhibiting a selective effect in relation to non-tumor cells. The microarray analysis showed changes in more than 253 genes involved in intracellular pathways related to the inflammatory response, growth factor activity and chemotaxis, which are associated with cancer development. Propolis exerted doxorubicin-like changes in gene expression. Conclusion: Propolis and Dox exerted a similar action, altering some pathways and indicating possible mechanisms of action involved in the cytotoxic effect on OSCA-8 cells. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-01 2023-07-29T14:32:27Z 2023-07-29T14:32:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.phyplu.2022.100356 Phytomedicine Plus, v. 2, n. 4, 2022. 2667-0313 http://hdl.handle.net/11449/249233 10.1016/j.phyplu.2022.100356 2-s2.0-85139360753 |
url |
http://dx.doi.org/10.1016/j.phyplu.2022.100356 http://hdl.handle.net/11449/249233 |
identifier_str_mv |
Phytomedicine Plus, v. 2, n. 4, 2022. 2667-0313 10.1016/j.phyplu.2022.100356 2-s2.0-85139360753 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Phytomedicine Plus |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129005819265024 |