Molecular detection ofHistoplasma capsulatumin bats of the Amazon biome in Para state, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/tbed.13740 http://hdl.handle.net/11449/197134 |
Resumo: | Histoplasma capsulatum, the fungus causing histoplasmosis, has a strong impact on public health. Histoplasmosis is one of the most prevalent systemic mycoses in the Americas and occurs in several mammalian species. Bats are important in the epidemiological cycle of histoplasmosis because they disseminate the fungus throughout the environment. The aim of the present study was to investigate naturalH. capsulatuminfection in bats located in forested areas, which have undergone anthropogenic perturbations, as well as in the urban areas of the state of Para. Twenty-two species of bats were captured in 18 municipalities of Para; the samples obtained from these animals were subjected to nested PCR for amplification ofH. capsulatumDNA. The HCI/HCII and HCIII/HCIV primers were used, and the final 210-pb fragment was amplified. Of the 100 bats analysed, two were confirmed to be positive forH. capsulatum. Samples amplified by nested PCR were sequenced and found to share identity and have 100% match withH. capsulatumDNA.H. capsulatumwas detected in the area of study: the state of Para has a wide diversity of bat species, and the region under investigation is situated in the north of the state, which suffers the most severe environmental and climatic changes. Therefore, it is essential to elucidate the distribution ofH. capsulatumhosts in this region to facilitate the implementation of effective disease surveillance. |
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Molecular detection ofHistoplasma capsulatumin bats of the Amazon biome in Para state, BrazilBrazilian Amazonchiropterafragmented areashistoplasmosismolecular biologyHistoplasma capsulatum, the fungus causing histoplasmosis, has a strong impact on public health. Histoplasmosis is one of the most prevalent systemic mycoses in the Americas and occurs in several mammalian species. Bats are important in the epidemiological cycle of histoplasmosis because they disseminate the fungus throughout the environment. The aim of the present study was to investigate naturalH. capsulatuminfection in bats located in forested areas, which have undergone anthropogenic perturbations, as well as in the urban areas of the state of Para. Twenty-two species of bats were captured in 18 municipalities of Para; the samples obtained from these animals were subjected to nested PCR for amplification ofH. capsulatumDNA. The HCI/HCII and HCIII/HCIV primers were used, and the final 210-pb fragment was amplified. Of the 100 bats analysed, two were confirmed to be positive forH. capsulatum. Samples amplified by nested PCR were sequenced and found to share identity and have 100% match withH. capsulatumDNA.H. capsulatumwas detected in the area of study: the state of Para has a wide diversity of bat species, and the region under investigation is situated in the north of the state, which suffers the most severe environmental and climatic changes. Therefore, it is essential to elucidate the distribution ofH. capsulatumhosts in this region to facilitate the implementation of effective disease surveillance.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)PROPESP / UFPAFed Univ Para UFPA, Inst Vet Med, Fac Vet Med, Ave Univ S-N, BR-68746360 Castanhal, PA, BrazilInst Tecnol Vale Desenvolvimento Sustentavel, Belem, Para, BrazilUniv Estadual Paulista, Dept Higiene Vet & Saude Publ, Campus Botucatu, Botucatu, SP, BrazilUniv Estadual Paulista, Dept Higiene Vet & Saude Publ, Campus Botucatu, Botucatu, SP, BrazilCNPq: 482743/2013-1CAPES: 001Wiley-BlackwellUniversidade Federal do Pará (UFPA)Inst Tecnol Vale Desenvolvimento SustentavelUniversidade Estadual Paulista (Unesp)Silva, Josileide Araujo daScofield, AlessandraBarros, Flavia de NazareFarias, Diana Maria deRiet-Correa, GabrielaBezerra Junior, Pedro SoaresSantos, Tiago Felipe SouzaTavares, Gabriel Savio FernandesTrevelin, Leonardo CarreiraPaz, Giselle Souza da [UNESP]Cerqueira, Valiria Duarte2020-12-10T20:07:17Z2020-12-10T20:07:17Z2020-08-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9http://dx.doi.org/10.1111/tbed.13740Transboundary And Emerging Diseases. Hoboken: Wiley, 9 p., 2020.1865-1674http://hdl.handle.net/11449/19713410.1111/tbed.13740WOS:000555278500001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTransboundary And Emerging Diseasesinfo:eu-repo/semantics/openAccess2021-10-23T11:59:39Zoai:repositorio.unesp.br:11449/197134Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T11:59:39Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Molecular detection ofHistoplasma capsulatumin bats of the Amazon biome in Para state, Brazil |
title |
Molecular detection ofHistoplasma capsulatumin bats of the Amazon biome in Para state, Brazil |
spellingShingle |
Molecular detection ofHistoplasma capsulatumin bats of the Amazon biome in Para state, Brazil Silva, Josileide Araujo da Brazilian Amazon chiroptera fragmented areas histoplasmosis molecular biology |
title_short |
Molecular detection ofHistoplasma capsulatumin bats of the Amazon biome in Para state, Brazil |
title_full |
Molecular detection ofHistoplasma capsulatumin bats of the Amazon biome in Para state, Brazil |
title_fullStr |
Molecular detection ofHistoplasma capsulatumin bats of the Amazon biome in Para state, Brazil |
title_full_unstemmed |
Molecular detection ofHistoplasma capsulatumin bats of the Amazon biome in Para state, Brazil |
title_sort |
Molecular detection ofHistoplasma capsulatumin bats of the Amazon biome in Para state, Brazil |
author |
Silva, Josileide Araujo da |
author_facet |
Silva, Josileide Araujo da Scofield, Alessandra Barros, Flavia de Nazare Farias, Diana Maria de Riet-Correa, Gabriela Bezerra Junior, Pedro Soares Santos, Tiago Felipe Souza Tavares, Gabriel Savio Fernandes Trevelin, Leonardo Carreira Paz, Giselle Souza da [UNESP] Cerqueira, Valiria Duarte |
author_role |
author |
author2 |
Scofield, Alessandra Barros, Flavia de Nazare Farias, Diana Maria de Riet-Correa, Gabriela Bezerra Junior, Pedro Soares Santos, Tiago Felipe Souza Tavares, Gabriel Savio Fernandes Trevelin, Leonardo Carreira Paz, Giselle Souza da [UNESP] Cerqueira, Valiria Duarte |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Pará (UFPA) Inst Tecnol Vale Desenvolvimento Sustentavel Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Silva, Josileide Araujo da Scofield, Alessandra Barros, Flavia de Nazare Farias, Diana Maria de Riet-Correa, Gabriela Bezerra Junior, Pedro Soares Santos, Tiago Felipe Souza Tavares, Gabriel Savio Fernandes Trevelin, Leonardo Carreira Paz, Giselle Souza da [UNESP] Cerqueira, Valiria Duarte |
dc.subject.por.fl_str_mv |
Brazilian Amazon chiroptera fragmented areas histoplasmosis molecular biology |
topic |
Brazilian Amazon chiroptera fragmented areas histoplasmosis molecular biology |
description |
Histoplasma capsulatum, the fungus causing histoplasmosis, has a strong impact on public health. Histoplasmosis is one of the most prevalent systemic mycoses in the Americas and occurs in several mammalian species. Bats are important in the epidemiological cycle of histoplasmosis because they disseminate the fungus throughout the environment. The aim of the present study was to investigate naturalH. capsulatuminfection in bats located in forested areas, which have undergone anthropogenic perturbations, as well as in the urban areas of the state of Para. Twenty-two species of bats were captured in 18 municipalities of Para; the samples obtained from these animals were subjected to nested PCR for amplification ofH. capsulatumDNA. The HCI/HCII and HCIII/HCIV primers were used, and the final 210-pb fragment was amplified. Of the 100 bats analysed, two were confirmed to be positive forH. capsulatum. Samples amplified by nested PCR were sequenced and found to share identity and have 100% match withH. capsulatumDNA.H. capsulatumwas detected in the area of study: the state of Para has a wide diversity of bat species, and the region under investigation is situated in the north of the state, which suffers the most severe environmental and climatic changes. Therefore, it is essential to elucidate the distribution ofH. capsulatumhosts in this region to facilitate the implementation of effective disease surveillance. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-10T20:07:17Z 2020-12-10T20:07:17Z 2020-08-04 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/tbed.13740 Transboundary And Emerging Diseases. Hoboken: Wiley, 9 p., 2020. 1865-1674 http://hdl.handle.net/11449/197134 10.1111/tbed.13740 WOS:000555278500001 |
url |
http://dx.doi.org/10.1111/tbed.13740 http://hdl.handle.net/11449/197134 |
identifier_str_mv |
Transboundary And Emerging Diseases. Hoboken: Wiley, 9 p., 2020. 1865-1674 10.1111/tbed.13740 WOS:000555278500001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Transboundary And Emerging Diseases |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1803650338977218560 |