Detection of immunoglobulin G antibodies to Neospora caninum in humans: High seropositivity rates in patients who are infected by human immunodeficiency virus or have neurological disorders
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1128/CVI.13.1.84-89.2006 http://hdl.handle.net/11449/39665 |
Resumo: | Considering that little is known about the epidemiology of Neospora caninum infection in humans, particularly in populations with high Toxoplasma gondii infection rates, the present study aimed to investigate the presence of antibodies to N. caninum in T. gondii-seropositive and -seronegative individuals. A total of 256 serum samples divided into four groups (61 samples from human immunodeficiency virus [HIV]-positive patients, 50 samples from patients with neurological disorders, 91 samples from newborns, and 54 samples from healthy subjects) were assessed for N. caninum and T. gondii serologies by indirect fluorescent-antibody test, enzyme-linked immunosorbent assay, and immunoblotting (IB). Immunoglobulin G antibodies to N. caninum were predominantly detected in HIV-infected patients (38%) and patients with neurological disorders (18%), while newborns and healthy subjects showed lower seropositivity rates (5% and 6%, respectively). Seropositivity to N. caninum was significantly associated with seropositivity to T. gondii in both HIV-infected patients and patients with neurological disorders. Seroreactivity to N. caninum was confirmed by IB, with positive sera predominantly recognizing the 29-kDa antigen of N. caninum. The results of this study indicate the presence of N. caninum infection or exposure in humans, particularly in HIV-infected patients or patients with neurological disorders, who could have opportunistic and concurrent infections with T. gondii. These findings may bring a new concern for the unstable clinical health of HIV-infected patients and the actual role of N. caninum infection in immunocompromised patients. |
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Detection of immunoglobulin G antibodies to Neospora caninum in humans: High seropositivity rates in patients who are infected by human immunodeficiency virus or have neurological disordersConsidering that little is known about the epidemiology of Neospora caninum infection in humans, particularly in populations with high Toxoplasma gondii infection rates, the present study aimed to investigate the presence of antibodies to N. caninum in T. gondii-seropositive and -seronegative individuals. A total of 256 serum samples divided into four groups (61 samples from human immunodeficiency virus [HIV]-positive patients, 50 samples from patients with neurological disorders, 91 samples from newborns, and 54 samples from healthy subjects) were assessed for N. caninum and T. gondii serologies by indirect fluorescent-antibody test, enzyme-linked immunosorbent assay, and immunoblotting (IB). Immunoglobulin G antibodies to N. caninum were predominantly detected in HIV-infected patients (38%) and patients with neurological disorders (18%), while newborns and healthy subjects showed lower seropositivity rates (5% and 6%, respectively). Seropositivity to N. caninum was significantly associated with seropositivity to T. gondii in both HIV-infected patients and patients with neurological disorders. Seroreactivity to N. caninum was confirmed by IB, with positive sera predominantly recognizing the 29-kDa antigen of N. caninum. The results of this study indicate the presence of N. caninum infection or exposure in humans, particularly in HIV-infected patients or patients with neurological disorders, who could have opportunistic and concurrent infections with T. gondii. These findings may bring a new concern for the unstable clinical health of HIV-infected patients and the actual role of N. caninum infection in immunocompromised patients.Universidade Federal de Uberlândia (UFU), Immunol Lab, BR-38400902 Uberlandia, MG, BrazilUniversidade Federal de Uberlândia (UFU), Parasitol Lab, BR-38400902 Uberlandia, MG, BrazilUniversidade Federal de Uberlândia (UFU), Clin Hosp, Sect Infect Dis, BR-38400902 Uberlandia, MG, BrazilUNESP, FCAV, Lab Immunoparasitol, Jaboticabal, SP, BrazilUNESP, FCAV, Lab Immunoparasitol, Jaboticabal, SP, BrazilAmer Soc MicrobiologyUniversidade Federal de Uberlândia (UFU)Universidade Estadual Paulista (Unesp)Lobato, J.Silva, DAOMineo, TWPAmaral, JDHFSegundo, GRSCosta-Cruz, J. M.Ferreira, M. S.Borges, A. S.Mineo, JR2014-05-20T15:30:14Z2014-05-20T15:30:14Z2006-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article84-89application/pdfhttp://dx.doi.org/10.1128/CVI.13.1.84-89.2006Clinical and Vaccine Immunology. Washington: Amer Soc Microbiology, v. 13, n. 1, p. 84-89, 2006.1556-6811http://hdl.handle.net/11449/3966510.1128/CVI.13.1.84-89.2006WOS:000235369300010WOS000235369300010.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical and Vaccine Immunology2.8721,320info:eu-repo/semantics/openAccess2024-06-07T13:03:09Zoai:repositorio.unesp.br:11449/39665Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-06-07T13:03:09Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Detection of immunoglobulin G antibodies to Neospora caninum in humans: High seropositivity rates in patients who are infected by human immunodeficiency virus or have neurological disorders |
title |
Detection of immunoglobulin G antibodies to Neospora caninum in humans: High seropositivity rates in patients who are infected by human immunodeficiency virus or have neurological disorders |
spellingShingle |
Detection of immunoglobulin G antibodies to Neospora caninum in humans: High seropositivity rates in patients who are infected by human immunodeficiency virus or have neurological disorders Lobato, J. |
title_short |
Detection of immunoglobulin G antibodies to Neospora caninum in humans: High seropositivity rates in patients who are infected by human immunodeficiency virus or have neurological disorders |
title_full |
Detection of immunoglobulin G antibodies to Neospora caninum in humans: High seropositivity rates in patients who are infected by human immunodeficiency virus or have neurological disorders |
title_fullStr |
Detection of immunoglobulin G antibodies to Neospora caninum in humans: High seropositivity rates in patients who are infected by human immunodeficiency virus or have neurological disorders |
title_full_unstemmed |
Detection of immunoglobulin G antibodies to Neospora caninum in humans: High seropositivity rates in patients who are infected by human immunodeficiency virus or have neurological disorders |
title_sort |
Detection of immunoglobulin G antibodies to Neospora caninum in humans: High seropositivity rates in patients who are infected by human immunodeficiency virus or have neurological disorders |
author |
Lobato, J. |
author_facet |
Lobato, J. Silva, DAO Mineo, TWP Amaral, JDHF Segundo, GRS Costa-Cruz, J. M. Ferreira, M. S. Borges, A. S. Mineo, JR |
author_role |
author |
author2 |
Silva, DAO Mineo, TWP Amaral, JDHF Segundo, GRS Costa-Cruz, J. M. Ferreira, M. S. Borges, A. S. Mineo, JR |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Uberlândia (UFU) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Lobato, J. Silva, DAO Mineo, TWP Amaral, JDHF Segundo, GRS Costa-Cruz, J. M. Ferreira, M. S. Borges, A. S. Mineo, JR |
description |
Considering that little is known about the epidemiology of Neospora caninum infection in humans, particularly in populations with high Toxoplasma gondii infection rates, the present study aimed to investigate the presence of antibodies to N. caninum in T. gondii-seropositive and -seronegative individuals. A total of 256 serum samples divided into four groups (61 samples from human immunodeficiency virus [HIV]-positive patients, 50 samples from patients with neurological disorders, 91 samples from newborns, and 54 samples from healthy subjects) were assessed for N. caninum and T. gondii serologies by indirect fluorescent-antibody test, enzyme-linked immunosorbent assay, and immunoblotting (IB). Immunoglobulin G antibodies to N. caninum were predominantly detected in HIV-infected patients (38%) and patients with neurological disorders (18%), while newborns and healthy subjects showed lower seropositivity rates (5% and 6%, respectively). Seropositivity to N. caninum was significantly associated with seropositivity to T. gondii in both HIV-infected patients and patients with neurological disorders. Seroreactivity to N. caninum was confirmed by IB, with positive sera predominantly recognizing the 29-kDa antigen of N. caninum. The results of this study indicate the presence of N. caninum infection or exposure in humans, particularly in HIV-infected patients or patients with neurological disorders, who could have opportunistic and concurrent infections with T. gondii. These findings may bring a new concern for the unstable clinical health of HIV-infected patients and the actual role of N. caninum infection in immunocompromised patients. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-01-01 2014-05-20T15:30:14Z 2014-05-20T15:30:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1128/CVI.13.1.84-89.2006 Clinical and Vaccine Immunology. Washington: Amer Soc Microbiology, v. 13, n. 1, p. 84-89, 2006. 1556-6811 http://hdl.handle.net/11449/39665 10.1128/CVI.13.1.84-89.2006 WOS:000235369300010 WOS000235369300010.pdf |
url |
http://dx.doi.org/10.1128/CVI.13.1.84-89.2006 http://hdl.handle.net/11449/39665 |
identifier_str_mv |
Clinical and Vaccine Immunology. Washington: Amer Soc Microbiology, v. 13, n. 1, p. 84-89, 2006. 1556-6811 10.1128/CVI.13.1.84-89.2006 WOS:000235369300010 WOS000235369300010.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Clinical and Vaccine Immunology 2.872 1,320 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
84-89 application/pdf |
dc.publisher.none.fl_str_mv |
Amer Soc Microbiology |
publisher.none.fl_str_mv |
Amer Soc Microbiology |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1803045564469739520 |