Strategy for reducing cytotoxicity and obtaining esthetic efficacy with 15 min of in-office dental bleaching
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00784-022-04379-z http://hdl.handle.net/11449/230436 |
Resumo: | Objectives: Evaluate in vitro the esthetic efficacy and cytotoxicity of a bleaching gel containing 35% hydrogen peroxide (BG-35%H2O2), applied for different time intervals, on enamel coated or not with polymeric biomaterials. Materials and methods: Nanofiber scaffolds (NSc) and a primer catalyst (PrCa) were used to coat the bovine enamel/dentin discs before the application of BG-35%H2O2, according to the following groups: G1—negative control (NC, without treatment); G2, G3, and G4—BG-35%H2O2 applied for 3 × 15, 2 × 15, and 15 min; G5, G6, and G7—BG-35%H2O2 applied on enamel coated with NSc and PrCa for 3 × 15; 2 × 15, and 15 min, respectively. The culture medium with components of gel diffused through the discs was applied on MDPC-23 cells, which were evaluated regarding to viability (VB), integrity of the membrane (IM), and oxidative stress (OxS). The quantity of H2O2 diffused and esthetic efficacy (ΔE/ΔWI) of the dental tissues were also analyzed (ANOVA/Tukey; p < 0.05). Results: Only G7 was similar to G1 regarding VB (p > 0.05). The lowest value of H2O2 diffusion occurred in G4 and G7, where the cells exhibited the lowest OxS than G2 (p < 0.05). Despite G5 showing the greatest ΔE regarding other groups (p < 0.05), the esthetic efficacy observed in G7 was similar to G2 (p > 0.05). ΔWI indicated a greater bleaching effect for groups G5, G6, and G7 (p < 0.05). Conclusion: Coating the dental enamel with polymeric biomaterials reduced the time and the cytotoxicity of BG-35%H2O2. Clinical significance. Coating the dental enamel with polymeric biomaterials allows safer and faster BG-35%H2O2 application. |
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Strategy for reducing cytotoxicity and obtaining esthetic efficacy with 15 min of in-office dental bleachingCytotoxicityDental bleachingOdontoblastsPolymeric biomaterialsObjectives: Evaluate in vitro the esthetic efficacy and cytotoxicity of a bleaching gel containing 35% hydrogen peroxide (BG-35%H2O2), applied for different time intervals, on enamel coated or not with polymeric biomaterials. Materials and methods: Nanofiber scaffolds (NSc) and a primer catalyst (PrCa) were used to coat the bovine enamel/dentin discs before the application of BG-35%H2O2, according to the following groups: G1—negative control (NC, without treatment); G2, G3, and G4—BG-35%H2O2 applied for 3 × 15, 2 × 15, and 15 min; G5, G6, and G7—BG-35%H2O2 applied on enamel coated with NSc and PrCa for 3 × 15; 2 × 15, and 15 min, respectively. The culture medium with components of gel diffused through the discs was applied on MDPC-23 cells, which were evaluated regarding to viability (VB), integrity of the membrane (IM), and oxidative stress (OxS). The quantity of H2O2 diffused and esthetic efficacy (ΔE/ΔWI) of the dental tissues were also analyzed (ANOVA/Tukey; p < 0.05). Results: Only G7 was similar to G1 regarding VB (p > 0.05). The lowest value of H2O2 diffusion occurred in G4 and G7, where the cells exhibited the lowest OxS than G2 (p < 0.05). Despite G5 showing the greatest ΔE regarding other groups (p < 0.05), the esthetic efficacy observed in G7 was similar to G2 (p > 0.05). ΔWI indicated a greater bleaching effect for groups G5, G6, and G7 (p < 0.05). Conclusion: Coating the dental enamel with polymeric biomaterials reduced the time and the cytotoxicity of BG-35%H2O2. Clinical significance. Coating the dental enamel with polymeric biomaterials allows safer and faster BG-35%H2O2 application.Department of Dental Materials and Prosthodontics Araraquara School of Dentistry São Paulo State University (Unesp), Rua Humaitá, 1680, SPDepartment of Operative Dentistry Endodontics and Dental Materials Bauru School of Dentistry University of São Paulo, Rua Alameda Dr. Octávio Pinheiro Brisolla, 9-75, SPDepartament of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry University São Paulo State (Unesp), Rua Humaitá, 1680, SPDepartment of Restorative Dentistry Araçatuba School of Dentistry University São Paulo State University (Unesp), Rua José Bonifácio 1193Department of Physiology and Pathology Araraquara School of Dentistry São Paulo State University (Unesp), Rua Humaitá, SPDepartment of Dental Materials and Prosthodontics Araraquara School of Dentistry São Paulo State University (Unesp), Rua Humaitá, 1680, SPDepartament of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry University São Paulo State (Unesp), Rua Humaitá, 1680, SPDepartment of Restorative Dentistry Araçatuba School of Dentistry University São Paulo State University (Unesp), Rua José Bonifácio 1193Department of Physiology and Pathology Araraquara School of Dentistry São Paulo State University (Unesp), Rua Humaitá, SPUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Ortecho-Zuta, Uxua [UNESP]de Oliveira Duque, Carla Caroline [UNESP]de Oliveira Ribeiro, Rafael Antonio [UNESP]Leite, Maria Luísa [UNESP]Soares, Diana GabrielaHebling, Josimeri [UNESP]Briso, André Luiz Fraga [UNESP]de Souza Costa, Carlos Alberto [UNESP]2022-04-29T08:39:56Z2022-04-29T08:39:56Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s00784-022-04379-zClinical Oral Investigations.1436-37711432-6981http://hdl.handle.net/11449/23043610.1007/s00784-022-04379-z2-s2.0-85125079660Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigationsinfo:eu-repo/semantics/openAccess2022-04-29T08:39:56Zoai:repositorio.unesp.br:11449/230436Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T08:39:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Strategy for reducing cytotoxicity and obtaining esthetic efficacy with 15 min of in-office dental bleaching |
title |
Strategy for reducing cytotoxicity and obtaining esthetic efficacy with 15 min of in-office dental bleaching |
spellingShingle |
Strategy for reducing cytotoxicity and obtaining esthetic efficacy with 15 min of in-office dental bleaching Ortecho-Zuta, Uxua [UNESP] Cytotoxicity Dental bleaching Odontoblasts Polymeric biomaterials |
title_short |
Strategy for reducing cytotoxicity and obtaining esthetic efficacy with 15 min of in-office dental bleaching |
title_full |
Strategy for reducing cytotoxicity and obtaining esthetic efficacy with 15 min of in-office dental bleaching |
title_fullStr |
Strategy for reducing cytotoxicity and obtaining esthetic efficacy with 15 min of in-office dental bleaching |
title_full_unstemmed |
Strategy for reducing cytotoxicity and obtaining esthetic efficacy with 15 min of in-office dental bleaching |
title_sort |
Strategy for reducing cytotoxicity and obtaining esthetic efficacy with 15 min of in-office dental bleaching |
author |
Ortecho-Zuta, Uxua [UNESP] |
author_facet |
Ortecho-Zuta, Uxua [UNESP] de Oliveira Duque, Carla Caroline [UNESP] de Oliveira Ribeiro, Rafael Antonio [UNESP] Leite, Maria Luísa [UNESP] Soares, Diana Gabriela Hebling, Josimeri [UNESP] Briso, André Luiz Fraga [UNESP] de Souza Costa, Carlos Alberto [UNESP] |
author_role |
author |
author2 |
de Oliveira Duque, Carla Caroline [UNESP] de Oliveira Ribeiro, Rafael Antonio [UNESP] Leite, Maria Luísa [UNESP] Soares, Diana Gabriela Hebling, Josimeri [UNESP] Briso, André Luiz Fraga [UNESP] de Souza Costa, Carlos Alberto [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Ortecho-Zuta, Uxua [UNESP] de Oliveira Duque, Carla Caroline [UNESP] de Oliveira Ribeiro, Rafael Antonio [UNESP] Leite, Maria Luísa [UNESP] Soares, Diana Gabriela Hebling, Josimeri [UNESP] Briso, André Luiz Fraga [UNESP] de Souza Costa, Carlos Alberto [UNESP] |
dc.subject.por.fl_str_mv |
Cytotoxicity Dental bleaching Odontoblasts Polymeric biomaterials |
topic |
Cytotoxicity Dental bleaching Odontoblasts Polymeric biomaterials |
description |
Objectives: Evaluate in vitro the esthetic efficacy and cytotoxicity of a bleaching gel containing 35% hydrogen peroxide (BG-35%H2O2), applied for different time intervals, on enamel coated or not with polymeric biomaterials. Materials and methods: Nanofiber scaffolds (NSc) and a primer catalyst (PrCa) were used to coat the bovine enamel/dentin discs before the application of BG-35%H2O2, according to the following groups: G1—negative control (NC, without treatment); G2, G3, and G4—BG-35%H2O2 applied for 3 × 15, 2 × 15, and 15 min; G5, G6, and G7—BG-35%H2O2 applied on enamel coated with NSc and PrCa for 3 × 15; 2 × 15, and 15 min, respectively. The culture medium with components of gel diffused through the discs was applied on MDPC-23 cells, which were evaluated regarding to viability (VB), integrity of the membrane (IM), and oxidative stress (OxS). The quantity of H2O2 diffused and esthetic efficacy (ΔE/ΔWI) of the dental tissues were also analyzed (ANOVA/Tukey; p < 0.05). Results: Only G7 was similar to G1 regarding VB (p > 0.05). The lowest value of H2O2 diffusion occurred in G4 and G7, where the cells exhibited the lowest OxS than G2 (p < 0.05). Despite G5 showing the greatest ΔE regarding other groups (p < 0.05), the esthetic efficacy observed in G7 was similar to G2 (p > 0.05). ΔWI indicated a greater bleaching effect for groups G5, G6, and G7 (p < 0.05). Conclusion: Coating the dental enamel with polymeric biomaterials reduced the time and the cytotoxicity of BG-35%H2O2. Clinical significance. Coating the dental enamel with polymeric biomaterials allows safer and faster BG-35%H2O2 application. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-29T08:39:56Z 2022-04-29T08:39:56Z 2022-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00784-022-04379-z Clinical Oral Investigations. 1436-3771 1432-6981 http://hdl.handle.net/11449/230436 10.1007/s00784-022-04379-z 2-s2.0-85125079660 |
url |
http://dx.doi.org/10.1007/s00784-022-04379-z http://hdl.handle.net/11449/230436 |
identifier_str_mv |
Clinical Oral Investigations. 1436-3771 1432-6981 10.1007/s00784-022-04379-z 2-s2.0-85125079660 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Clinical Oral Investigations |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046755535683584 |