Raw milk cheese as a potential infection source of pathogenic and toxigenic food born pathogens

Detalhes bibliográficos
Autor(a) principal: Cardozo, Marita Vedovelli [UNESP]
Data de Publicação: 2021
Outros Autores: Nespolo, Natalia, Delfino, Tammy Chioda [UNESP], de ALMEIDA, Camila Chioda [UNESP], Pizauro, Lucas José Luduverio [UNESP], Valmorbida, Mylena Karoline [UNESP], Pereira, Natália [UNESP], de ÁVILA, Fernando Antônio [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/fst.37919
http://hdl.handle.net/11449/207553
Resumo: The consumption of raw milk cheese has been growing worldwide with S. aureus and E. coli been the leading agents in food poisoning. The present work aims to evaluate the microbiological quality of raw milk cheeses commercialized in Brazil, regarding microbiology safety and enterotoxin gene presence. Forty-three raw milk cheeses samples from five different suppliers were analyzed. Counting and identification of S. aureus, E. coli and Salmonella spp were performed according to BAM from the FDA. Further S. aureus identification was performed by the cydB and Salmonella spp by the invA gene. S. aureus toxin genes (sea, seb, sec, see, ses, seh and sei) and E. coli gene LT, STa, Stb, stx1, stx2, eae, rmpA, wabG, mrkD, kfu, mcgA, fimH and uge were analysed. From the 43 samples analyzed, 18 presented S. aureus with two isolates positive for the tst gene, two for the sec gene, two for the seh gene and four for the sei gene. Thirty-five E. coli and seven. Salmonella spp isolates were obtained. E. coli isolates harbored sta and stx2 genes. The results revealed that raw milk cheeses sold can cause harm consumer’s health and highlights the importance of adoption good hygienic-sanitary practices and consumers awareness.
id UNSP_6f3b952be9d8fead62c53f60fbf79654
oai_identifier_str oai:repositorio.unesp.br:11449/207553
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Raw milk cheese as a potential infection source of pathogenic and toxigenic food born pathogensEpidemiologyFood safetyPathogensVirulence genesThe consumption of raw milk cheese has been growing worldwide with S. aureus and E. coli been the leading agents in food poisoning. The present work aims to evaluate the microbiological quality of raw milk cheeses commercialized in Brazil, regarding microbiology safety and enterotoxin gene presence. Forty-three raw milk cheeses samples from five different suppliers were analyzed. Counting and identification of S. aureus, E. coli and Salmonella spp were performed according to BAM from the FDA. Further S. aureus identification was performed by the cydB and Salmonella spp by the invA gene. S. aureus toxin genes (sea, seb, sec, see, ses, seh and sei) and E. coli gene LT, STa, Stb, stx1, stx2, eae, rmpA, wabG, mrkD, kfu, mcgA, fimH and uge were analysed. From the 43 samples analyzed, 18 presented S. aureus with two isolates positive for the tst gene, two for the sec gene, two for the seh gene and four for the sei gene. Thirty-five E. coli and seven. Salmonella spp isolates were obtained. E. coli isolates harbored sta and stx2 genes. The results revealed that raw milk cheeses sold can cause harm consumer’s health and highlights the importance of adoption good hygienic-sanitary practices and consumers awareness.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Estadual PaulistaDepartamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista-UNESPNúcleo de Graduação em Medicina Veterinária Universidade Federal de SergipeColégio Técnico Agrícola “José Bonifácio” Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista-UNESPDepartamento de Tecnologia Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista-UNESPDepartamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista-UNESPColégio Técnico Agrícola “José Bonifácio” Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista-UNESPDepartamento de Tecnologia Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista-UNESPUniversidade Estadual Paulista (Unesp)Universidade Federal de Sergipe (UFS)Cardozo, Marita Vedovelli [UNESP]Nespolo, NataliaDelfino, Tammy Chioda [UNESP]de ALMEIDA, Camila Chioda [UNESP]Pizauro, Lucas José Luduverio [UNESP]Valmorbida, Mylena Karoline [UNESP]Pereira, Natália [UNESP]de ÁVILA, Fernando Antônio [UNESP]2021-06-25T10:57:07Z2021-06-25T10:57:07Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article355-358http://dx.doi.org/10.1590/fst.37919Food Science and Technology (Brazil), v. 41, n. 2, p. 355-358, 2021.1678-457Xhttp://hdl.handle.net/11449/20755310.1590/fst.379192-s2.0-85103615024Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFood Science and Technology (Brazil)info:eu-repo/semantics/openAccess2024-06-07T15:31:35Zoai:repositorio.unesp.br:11449/207553Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:20:16.807268Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Raw milk cheese as a potential infection source of pathogenic and toxigenic food born pathogens
title Raw milk cheese as a potential infection source of pathogenic and toxigenic food born pathogens
spellingShingle Raw milk cheese as a potential infection source of pathogenic and toxigenic food born pathogens
Cardozo, Marita Vedovelli [UNESP]
Epidemiology
Food safety
Pathogens
Virulence genes
title_short Raw milk cheese as a potential infection source of pathogenic and toxigenic food born pathogens
title_full Raw milk cheese as a potential infection source of pathogenic and toxigenic food born pathogens
title_fullStr Raw milk cheese as a potential infection source of pathogenic and toxigenic food born pathogens
title_full_unstemmed Raw milk cheese as a potential infection source of pathogenic and toxigenic food born pathogens
title_sort Raw milk cheese as a potential infection source of pathogenic and toxigenic food born pathogens
author Cardozo, Marita Vedovelli [UNESP]
author_facet Cardozo, Marita Vedovelli [UNESP]
Nespolo, Natalia
Delfino, Tammy Chioda [UNESP]
de ALMEIDA, Camila Chioda [UNESP]
Pizauro, Lucas José Luduverio [UNESP]
Valmorbida, Mylena Karoline [UNESP]
Pereira, Natália [UNESP]
de ÁVILA, Fernando Antônio [UNESP]
author_role author
author2 Nespolo, Natalia
Delfino, Tammy Chioda [UNESP]
de ALMEIDA, Camila Chioda [UNESP]
Pizauro, Lucas José Luduverio [UNESP]
Valmorbida, Mylena Karoline [UNESP]
Pereira, Natália [UNESP]
de ÁVILA, Fernando Antônio [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal de Sergipe (UFS)
dc.contributor.author.fl_str_mv Cardozo, Marita Vedovelli [UNESP]
Nespolo, Natalia
Delfino, Tammy Chioda [UNESP]
de ALMEIDA, Camila Chioda [UNESP]
Pizauro, Lucas José Luduverio [UNESP]
Valmorbida, Mylena Karoline [UNESP]
Pereira, Natália [UNESP]
de ÁVILA, Fernando Antônio [UNESP]
dc.subject.por.fl_str_mv Epidemiology
Food safety
Pathogens
Virulence genes
topic Epidemiology
Food safety
Pathogens
Virulence genes
description The consumption of raw milk cheese has been growing worldwide with S. aureus and E. coli been the leading agents in food poisoning. The present work aims to evaluate the microbiological quality of raw milk cheeses commercialized in Brazil, regarding microbiology safety and enterotoxin gene presence. Forty-three raw milk cheeses samples from five different suppliers were analyzed. Counting and identification of S. aureus, E. coli and Salmonella spp were performed according to BAM from the FDA. Further S. aureus identification was performed by the cydB and Salmonella spp by the invA gene. S. aureus toxin genes (sea, seb, sec, see, ses, seh and sei) and E. coli gene LT, STa, Stb, stx1, stx2, eae, rmpA, wabG, mrkD, kfu, mcgA, fimH and uge were analysed. From the 43 samples analyzed, 18 presented S. aureus with two isolates positive for the tst gene, two for the sec gene, two for the seh gene and four for the sei gene. Thirty-five E. coli and seven. Salmonella spp isolates were obtained. E. coli isolates harbored sta and stx2 genes. The results revealed that raw milk cheeses sold can cause harm consumer’s health and highlights the importance of adoption good hygienic-sanitary practices and consumers awareness.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T10:57:07Z
2021-06-25T10:57:07Z
2021-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/fst.37919
Food Science and Technology (Brazil), v. 41, n. 2, p. 355-358, 2021.
1678-457X
http://hdl.handle.net/11449/207553
10.1590/fst.37919
2-s2.0-85103615024
url http://dx.doi.org/10.1590/fst.37919
http://hdl.handle.net/11449/207553
identifier_str_mv Food Science and Technology (Brazil), v. 41, n. 2, p. 355-358, 2021.
1678-457X
10.1590/fst.37919
2-s2.0-85103615024
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Food Science and Technology (Brazil)
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 355-358
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128635269283840