Interaction between Lactobacillus reuteri and periodontopathogenic bacteria using in vitro and in vivo (G. mellonella) approaches

Detalhes bibliográficos
Autor(a) principal: Santos, Thaís Aguiar
Data de Publicação: 2020
Outros Autores: Scorzoni, Liliana, Correia, Raquel, Junqueira, Juliana Campos, Anbinder, Ana Lia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/202514
Resumo: Periodontitis is a multifactorial inflammatory disease, and the major cause of tooth loss in adults. New therapies have been proposed for its treatment, including the use of probiotics such as Lactobacillus reuteri. The objective of this study was to evaluate the antimicrobial effects of L. reuteri: live, heat-killed and culture filtrate (cell-free supernatant), on periodontopathogenic bacteria (Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans) in vitro, as well as the in vivo survival curve, hemocyte density and microbial recovery using Galleria mellonella. For in vitro assays, all preparations reduced colony forming units of F. nucleatum, while only live L. reuteri reduced the growth of A. actinomycetemcomitans. All treatments reduced periodontopathogenic bacteria growth in vivo. The treatment with the supernatant increased the survival of larvae infected with F. nucleatum more than the treatment with live L. reuteri, and none of the treatments altered the survival of A. actinomycetemcomitans-infected larvae. In addition, the treatment with L. reuteri preparations did not alter the hemocyte count of F. nucleatum- and A. actinomycetemcomitans-infected larvae. This study demonstrated that L. reuteri preparations exerted antimicrobial effects and increased the survival of G. mellonella infected by F. nucleatum, although only live L. reuteri was able to reduce the growth of A. actinomycetemcomitans in vitro.
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spelling Interaction between Lactobacillus reuteri and periodontopathogenic bacteria using in vitro and in vivo (G. mellonella) approachesPeriodontal diseasesProbioticsFusobacterium nucleatumAggregatibacter actinomycetemcomitansLactobacillus reuteriGalleria mellonellaPeriodontitis is a multifactorial inflammatory disease, and the major cause of tooth loss in adults. New therapies have been proposed for its treatment, including the use of probiotics such as Lactobacillus reuteri. The objective of this study was to evaluate the antimicrobial effects of L. reuteri: live, heat-killed and culture filtrate (cell-free supernatant), on periodontopathogenic bacteria (Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans) in vitro, as well as the in vivo survival curve, hemocyte density and microbial recovery using Galleria mellonella. For in vitro assays, all preparations reduced colony forming units of F. nucleatum, while only live L. reuteri reduced the growth of A. actinomycetemcomitans. All treatments reduced periodontopathogenic bacteria growth in vivo. The treatment with the supernatant increased the survival of larvae infected with F. nucleatum more than the treatment with live L. reuteri, and none of the treatments altered the survival of A. actinomycetemcomitans-infected larvae. In addition, the treatment with L. reuteri preparations did not alter the hemocyte count of F. nucleatum- and A. actinomycetemcomitans-infected larvae. This study demonstrated that L. reuteri preparations exerted antimicrobial effects and increased the survival of G. mellonella infected by F. nucleatum, although only live L. reuteri was able to reduce the growth of A. actinomycetemcomitans in vitro.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)PreprintUniversidade Estadual Paulista Julio de Mesquita FilhoFAPESP: 2016/06946-1FAPESP: 2017/26461-5Oxford AcademicUniversidade Estadual Paulista (Unesp)Santos, Thaís AguiarScorzoni, LilianaCorreia, RaquelJunqueira, Juliana CamposAnbinder, Ana Lia2021-01-26T18:58:04Z2021-01-26T18:58:04Z2020-08-26info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdf2049-632Xhttp://hdl.handle.net/11449/20251410.1093/femspd/ftaa04471411548585129106211259014916418032202054105590060979679430082730000-0002-5762-11450000-0003-3930-4274engPathogens and diseaseinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESP2023-11-16T06:09:58Zoai:repositorio.unesp.br:11449/202514Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-16T06:09:58Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Interaction between Lactobacillus reuteri and periodontopathogenic bacteria using in vitro and in vivo (G. mellonella) approaches
title Interaction between Lactobacillus reuteri and periodontopathogenic bacteria using in vitro and in vivo (G. mellonella) approaches
spellingShingle Interaction between Lactobacillus reuteri and periodontopathogenic bacteria using in vitro and in vivo (G. mellonella) approaches
Santos, Thaís Aguiar
Periodontal diseases
Probiotics
Fusobacterium nucleatum
Aggregatibacter actinomycetemcomitans
Lactobacillus reuteri
Galleria mellonella
title_short Interaction between Lactobacillus reuteri and periodontopathogenic bacteria using in vitro and in vivo (G. mellonella) approaches
title_full Interaction between Lactobacillus reuteri and periodontopathogenic bacteria using in vitro and in vivo (G. mellonella) approaches
title_fullStr Interaction between Lactobacillus reuteri and periodontopathogenic bacteria using in vitro and in vivo (G. mellonella) approaches
title_full_unstemmed Interaction between Lactobacillus reuteri and periodontopathogenic bacteria using in vitro and in vivo (G. mellonella) approaches
title_sort Interaction between Lactobacillus reuteri and periodontopathogenic bacteria using in vitro and in vivo (G. mellonella) approaches
author Santos, Thaís Aguiar
author_facet Santos, Thaís Aguiar
Scorzoni, Liliana
Correia, Raquel
Junqueira, Juliana Campos
Anbinder, Ana Lia
author_role author
author2 Scorzoni, Liliana
Correia, Raquel
Junqueira, Juliana Campos
Anbinder, Ana Lia
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Santos, Thaís Aguiar
Scorzoni, Liliana
Correia, Raquel
Junqueira, Juliana Campos
Anbinder, Ana Lia
dc.subject.por.fl_str_mv Periodontal diseases
Probiotics
Fusobacterium nucleatum
Aggregatibacter actinomycetemcomitans
Lactobacillus reuteri
Galleria mellonella
topic Periodontal diseases
Probiotics
Fusobacterium nucleatum
Aggregatibacter actinomycetemcomitans
Lactobacillus reuteri
Galleria mellonella
description Periodontitis is a multifactorial inflammatory disease, and the major cause of tooth loss in adults. New therapies have been proposed for its treatment, including the use of probiotics such as Lactobacillus reuteri. The objective of this study was to evaluate the antimicrobial effects of L. reuteri: live, heat-killed and culture filtrate (cell-free supernatant), on periodontopathogenic bacteria (Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans) in vitro, as well as the in vivo survival curve, hemocyte density and microbial recovery using Galleria mellonella. For in vitro assays, all preparations reduced colony forming units of F. nucleatum, while only live L. reuteri reduced the growth of A. actinomycetemcomitans. All treatments reduced periodontopathogenic bacteria growth in vivo. The treatment with the supernatant increased the survival of larvae infected with F. nucleatum more than the treatment with live L. reuteri, and none of the treatments altered the survival of A. actinomycetemcomitans-infected larvae. In addition, the treatment with L. reuteri preparations did not alter the hemocyte count of F. nucleatum- and A. actinomycetemcomitans-infected larvae. This study demonstrated that L. reuteri preparations exerted antimicrobial effects and increased the survival of G. mellonella infected by F. nucleatum, although only live L. reuteri was able to reduce the growth of A. actinomycetemcomitans in vitro.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-26
2021-01-26T18:58:04Z
2021-01-26T18:58:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv 2049-632X
http://hdl.handle.net/11449/202514
10.1093/femspd/ftaa044
7141154858512910
6211259014916418
0322020541055900
6097967943008273
0000-0002-5762-1145
0000-0003-3930-4274
identifier_str_mv 2049-632X
10.1093/femspd/ftaa044
7141154858512910
6211259014916418
0322020541055900
6097967943008273
0000-0002-5762-1145
0000-0003-3930-4274
url http://hdl.handle.net/11449/202514
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pathogens and disease
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford Academic
publisher.none.fl_str_mv Oxford Academic
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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