Favourable effects of Bacillus subtilis and Bacillus licheniformis on experimental periodontitis in rats
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.archoralbio.2016.02.014 http://hdl.handle.net/11449/172631 |
Resumo: | Objectives The purposes of this study were to evaluate, in rats: i) the effects of Bacillus species on the development of experimental periodontitis (EP) via microtomographic, immunological and hematological assays (Experiment 1 - E1); ii) the effects of Bacillus species as adjuncts to scaling and root planing (SRP) for the treatment of EP via histomorphometric and immunohistochemical analyses (Experiment 2 - E2). Methods In E1, 24 rats were divided into groups C1 (control), PROB1, EP1 and EP-PROB1. In groups with EP, the mandibular first molar of each animal received a ligature for 14 days. In groups PROB1, animals received Bacillus species for 44 days, starting 30 days before EP induction in Group EP-PROB1. In E2, 24 rats were assigned to groups C2 (control), PROB2, EP-SRP2 and EP-SRP-PROB2. In groups with SRP, EP was induced as described in E1. The ligatures were removed after 14 days and SRP was performed. In groups PROB2, animals received Bacillus species for 15 days, starting after SRP in Group EP-SRP-PROB2. Results In E1, Group EP1 presented bone loss (BL) and eosinophil numbers greater than Group EP-PROB1 (P < 0.05). In Group EP-PROB1, the receptor activator of nuclear factor-kB ligand (RANKL)/osteoprotegerin (OPG) ratio was similar to that of groups without EP. In E2, Group EP-SRP-PROB2 presented fewer TRAP-positive osteoclasts, lower immunolabeling pattern for a proinflammatory cytokine and decreased BL and attachment loss than Group EP-SRP2 (P < 0.05). Conclusions Bacillus species supplementation provided a protective effect against BL and enhanced the effects of SRP in the treatment of EP in rats. |
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Favourable effects of Bacillus subtilis and Bacillus licheniformis on experimental periodontitis in ratsPeriodontitisProbioticsRatsRoot planingObjectives The purposes of this study were to evaluate, in rats: i) the effects of Bacillus species on the development of experimental periodontitis (EP) via microtomographic, immunological and hematological assays (Experiment 1 - E1); ii) the effects of Bacillus species as adjuncts to scaling and root planing (SRP) for the treatment of EP via histomorphometric and immunohistochemical analyses (Experiment 2 - E2). Methods In E1, 24 rats were divided into groups C1 (control), PROB1, EP1 and EP-PROB1. In groups with EP, the mandibular first molar of each animal received a ligature for 14 days. In groups PROB1, animals received Bacillus species for 44 days, starting 30 days before EP induction in Group EP-PROB1. In E2, 24 rats were assigned to groups C2 (control), PROB2, EP-SRP2 and EP-SRP-PROB2. In groups with SRP, EP was induced as described in E1. The ligatures were removed after 14 days and SRP was performed. In groups PROB2, animals received Bacillus species for 15 days, starting after SRP in Group EP-SRP-PROB2. Results In E1, Group EP1 presented bone loss (BL) and eosinophil numbers greater than Group EP-PROB1 (P < 0.05). In Group EP-PROB1, the receptor activator of nuclear factor-kB ligand (RANKL)/osteoprotegerin (OPG) ratio was similar to that of groups without EP. In E2, Group EP-SRP-PROB2 presented fewer TRAP-positive osteoclasts, lower immunolabeling pattern for a proinflammatory cytokine and decreased BL and attachment loss than Group EP-SRP2 (P < 0.05). Conclusions Bacillus species supplementation provided a protective effect against BL and enhanced the effects of SRP in the treatment of EP in rats.Department of Oral and Maxillofacial Surgery and Periodontology School of Dentistry of Ribeirao Preto University of Sao Paulo - USP, Av. CaféPhysiology and Pharmacology Department Federal University of Lavras, Av. Doutor Sylvio Menicucci, 1001Laboratory of Immunology and Molecular Biology Sao Leopoldo Mandic, Street Dr. José Rocha Junqueira, 13Department of Surgery Integrated Clinic Dental School of Aracatuba UNESP-Univ Estadual Paulista, Street José Bonifácio, 1193Department of Basic Sciences Division of Histology Dental School of Aracatuba UNESP-Univ Estadual Paulista, Rodovia Marechal Rondon, km 527Department of Surgery Integrated Clinic Dental School of Aracatuba UNESP-Univ Estadual Paulista, Street José Bonifácio, 1193Department of Basic Sciences Division of Histology Dental School of Aracatuba UNESP-Univ Estadual Paulista, Rodovia Marechal Rondon, km 527Universidade de São Paulo (USP)Federal University of LavrasSao Leopoldo MandicUniversidade Estadual Paulista (Unesp)Messora, Michel R.Pereira, Luciano J.Foureaux, RenataOliveira, Luiz F.F.Sordi, Caio G.Alves, Ana J.N.Napimoga, Marcelo H.Nagata, Maria J.H. [UNESP]Ervolino, Edilson [UNESP]Furlaneto, Flávia A.C.2018-12-11T17:01:31Z2018-12-11T17:01:31Z2016-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article108-119application/pdfhttp://dx.doi.org/10.1016/j.archoralbio.2016.02.014Archives of Oral Biology, v. 66, p. 108-119.1879-15060003-9969http://hdl.handle.net/11449/17263110.1016/j.archoralbio.2016.02.0142-s2.0-849595444832-s2.0-84959544483.pdf44080955173468460000-0003-4859-0583Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biology0,752info:eu-repo/semantics/openAccess2024-09-19T14:02:46Zoai:repositorio.unesp.br:11449/172631Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T14:02:46Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Favourable effects of Bacillus subtilis and Bacillus licheniformis on experimental periodontitis in rats |
title |
Favourable effects of Bacillus subtilis and Bacillus licheniformis on experimental periodontitis in rats |
spellingShingle |
Favourable effects of Bacillus subtilis and Bacillus licheniformis on experimental periodontitis in rats Messora, Michel R. Periodontitis Probiotics Rats Root planing |
title_short |
Favourable effects of Bacillus subtilis and Bacillus licheniformis on experimental periodontitis in rats |
title_full |
Favourable effects of Bacillus subtilis and Bacillus licheniformis on experimental periodontitis in rats |
title_fullStr |
Favourable effects of Bacillus subtilis and Bacillus licheniformis on experimental periodontitis in rats |
title_full_unstemmed |
Favourable effects of Bacillus subtilis and Bacillus licheniformis on experimental periodontitis in rats |
title_sort |
Favourable effects of Bacillus subtilis and Bacillus licheniformis on experimental periodontitis in rats |
author |
Messora, Michel R. |
author_facet |
Messora, Michel R. Pereira, Luciano J. Foureaux, Renata Oliveira, Luiz F.F. Sordi, Caio G. Alves, Ana J.N. Napimoga, Marcelo H. Nagata, Maria J.H. [UNESP] Ervolino, Edilson [UNESP] Furlaneto, Flávia A.C. |
author_role |
author |
author2 |
Pereira, Luciano J. Foureaux, Renata Oliveira, Luiz F.F. Sordi, Caio G. Alves, Ana J.N. Napimoga, Marcelo H. Nagata, Maria J.H. [UNESP] Ervolino, Edilson [UNESP] Furlaneto, Flávia A.C. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Federal University of Lavras Sao Leopoldo Mandic Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Messora, Michel R. Pereira, Luciano J. Foureaux, Renata Oliveira, Luiz F.F. Sordi, Caio G. Alves, Ana J.N. Napimoga, Marcelo H. Nagata, Maria J.H. [UNESP] Ervolino, Edilson [UNESP] Furlaneto, Flávia A.C. |
dc.subject.por.fl_str_mv |
Periodontitis Probiotics Rats Root planing |
topic |
Periodontitis Probiotics Rats Root planing |
description |
Objectives The purposes of this study were to evaluate, in rats: i) the effects of Bacillus species on the development of experimental periodontitis (EP) via microtomographic, immunological and hematological assays (Experiment 1 - E1); ii) the effects of Bacillus species as adjuncts to scaling and root planing (SRP) for the treatment of EP via histomorphometric and immunohistochemical analyses (Experiment 2 - E2). Methods In E1, 24 rats were divided into groups C1 (control), PROB1, EP1 and EP-PROB1. In groups with EP, the mandibular first molar of each animal received a ligature for 14 days. In groups PROB1, animals received Bacillus species for 44 days, starting 30 days before EP induction in Group EP-PROB1. In E2, 24 rats were assigned to groups C2 (control), PROB2, EP-SRP2 and EP-SRP-PROB2. In groups with SRP, EP was induced as described in E1. The ligatures were removed after 14 days and SRP was performed. In groups PROB2, animals received Bacillus species for 15 days, starting after SRP in Group EP-SRP-PROB2. Results In E1, Group EP1 presented bone loss (BL) and eosinophil numbers greater than Group EP-PROB1 (P < 0.05). In Group EP-PROB1, the receptor activator of nuclear factor-kB ligand (RANKL)/osteoprotegerin (OPG) ratio was similar to that of groups without EP. In E2, Group EP-SRP-PROB2 presented fewer TRAP-positive osteoclasts, lower immunolabeling pattern for a proinflammatory cytokine and decreased BL and attachment loss than Group EP-SRP2 (P < 0.05). Conclusions Bacillus species supplementation provided a protective effect against BL and enhanced the effects of SRP in the treatment of EP in rats. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-06-01 2018-12-11T17:01:31Z 2018-12-11T17:01:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.archoralbio.2016.02.014 Archives of Oral Biology, v. 66, p. 108-119. 1879-1506 0003-9969 http://hdl.handle.net/11449/172631 10.1016/j.archoralbio.2016.02.014 2-s2.0-84959544483 2-s2.0-84959544483.pdf 4408095517346846 0000-0003-4859-0583 |
url |
http://dx.doi.org/10.1016/j.archoralbio.2016.02.014 http://hdl.handle.net/11449/172631 |
identifier_str_mv |
Archives of Oral Biology, v. 66, p. 108-119. 1879-1506 0003-9969 10.1016/j.archoralbio.2016.02.014 2-s2.0-84959544483 2-s2.0-84959544483.pdf 4408095517346846 0000-0003-4859-0583 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Oral Biology 0,752 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
108-119 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546441469067264 |