Favourable effects of Bacillus subtilis and Bacillus licheniformis on experimental periodontitis in rats

Detalhes bibliográficos
Autor(a) principal: Messora, Michel R.
Data de Publicação: 2016
Outros Autores: Pereira, Luciano J., Foureaux, Renata, Oliveira, Luiz F.F., Sordi, Caio G., Alves, Ana J.N., Napimoga, Marcelo H., Nagata, Maria J.H. [UNESP], Ervolino, Edilson [UNESP], Furlaneto, Flávia A.C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.archoralbio.2016.02.014
http://hdl.handle.net/11449/172631
Resumo: Objectives The purposes of this study were to evaluate, in rats: i) the effects of Bacillus species on the development of experimental periodontitis (EP) via microtomographic, immunological and hematological assays (Experiment 1 - E1); ii) the effects of Bacillus species as adjuncts to scaling and root planing (SRP) for the treatment of EP via histomorphometric and immunohistochemical analyses (Experiment 2 - E2). Methods In E1, 24 rats were divided into groups C1 (control), PROB1, EP1 and EP-PROB1. In groups with EP, the mandibular first molar of each animal received a ligature for 14 days. In groups PROB1, animals received Bacillus species for 44 days, starting 30 days before EP induction in Group EP-PROB1. In E2, 24 rats were assigned to groups C2 (control), PROB2, EP-SRP2 and EP-SRP-PROB2. In groups with SRP, EP was induced as described in E1. The ligatures were removed after 14 days and SRP was performed. In groups PROB2, animals received Bacillus species for 15 days, starting after SRP in Group EP-SRP-PROB2. Results In E1, Group EP1 presented bone loss (BL) and eosinophil numbers greater than Group EP-PROB1 (P < 0.05). In Group EP-PROB1, the receptor activator of nuclear factor-kB ligand (RANKL)/osteoprotegerin (OPG) ratio was similar to that of groups without EP. In E2, Group EP-SRP-PROB2 presented fewer TRAP-positive osteoclasts, lower immunolabeling pattern for a proinflammatory cytokine and decreased BL and attachment loss than Group EP-SRP2 (P < 0.05). Conclusions Bacillus species supplementation provided a protective effect against BL and enhanced the effects of SRP in the treatment of EP in rats.
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spelling Favourable effects of Bacillus subtilis and Bacillus licheniformis on experimental periodontitis in ratsPeriodontitisProbioticsRatsRoot planingObjectives The purposes of this study were to evaluate, in rats: i) the effects of Bacillus species on the development of experimental periodontitis (EP) via microtomographic, immunological and hematological assays (Experiment 1 - E1); ii) the effects of Bacillus species as adjuncts to scaling and root planing (SRP) for the treatment of EP via histomorphometric and immunohistochemical analyses (Experiment 2 - E2). Methods In E1, 24 rats were divided into groups C1 (control), PROB1, EP1 and EP-PROB1. In groups with EP, the mandibular first molar of each animal received a ligature for 14 days. In groups PROB1, animals received Bacillus species for 44 days, starting 30 days before EP induction in Group EP-PROB1. In E2, 24 rats were assigned to groups C2 (control), PROB2, EP-SRP2 and EP-SRP-PROB2. In groups with SRP, EP was induced as described in E1. The ligatures were removed after 14 days and SRP was performed. In groups PROB2, animals received Bacillus species for 15 days, starting after SRP in Group EP-SRP-PROB2. Results In E1, Group EP1 presented bone loss (BL) and eosinophil numbers greater than Group EP-PROB1 (P < 0.05). In Group EP-PROB1, the receptor activator of nuclear factor-kB ligand (RANKL)/osteoprotegerin (OPG) ratio was similar to that of groups without EP. In E2, Group EP-SRP-PROB2 presented fewer TRAP-positive osteoclasts, lower immunolabeling pattern for a proinflammatory cytokine and decreased BL and attachment loss than Group EP-SRP2 (P < 0.05). Conclusions Bacillus species supplementation provided a protective effect against BL and enhanced the effects of SRP in the treatment of EP in rats.Department of Oral and Maxillofacial Surgery and Periodontology School of Dentistry of Ribeirao Preto University of Sao Paulo - USP, Av. CaféPhysiology and Pharmacology Department Federal University of Lavras, Av. Doutor Sylvio Menicucci, 1001Laboratory of Immunology and Molecular Biology Sao Leopoldo Mandic, Street Dr. José Rocha Junqueira, 13Department of Surgery Integrated Clinic Dental School of Aracatuba UNESP-Univ Estadual Paulista, Street José Bonifácio, 1193Department of Basic Sciences Division of Histology Dental School of Aracatuba UNESP-Univ Estadual Paulista, Rodovia Marechal Rondon, km 527Department of Surgery Integrated Clinic Dental School of Aracatuba UNESP-Univ Estadual Paulista, Street José Bonifácio, 1193Department of Basic Sciences Division of Histology Dental School of Aracatuba UNESP-Univ Estadual Paulista, Rodovia Marechal Rondon, km 527Universidade de São Paulo (USP)Federal University of LavrasSao Leopoldo MandicUniversidade Estadual Paulista (Unesp)Messora, Michel R.Pereira, Luciano J.Foureaux, RenataOliveira, Luiz F.F.Sordi, Caio G.Alves, Ana J.N.Napimoga, Marcelo H.Nagata, Maria J.H. [UNESP]Ervolino, Edilson [UNESP]Furlaneto, Flávia A.C.2018-12-11T17:01:31Z2018-12-11T17:01:31Z2016-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article108-119application/pdfhttp://dx.doi.org/10.1016/j.archoralbio.2016.02.014Archives of Oral Biology, v. 66, p. 108-119.1879-15060003-9969http://hdl.handle.net/11449/17263110.1016/j.archoralbio.2016.02.0142-s2.0-849595444832-s2.0-84959544483.pdf44080955173468460000-0003-4859-0583Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biology0,752info:eu-repo/semantics/openAccess2024-09-19T14:02:46Zoai:repositorio.unesp.br:11449/172631Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T14:02:46Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Favourable effects of Bacillus subtilis and Bacillus licheniformis on experimental periodontitis in rats
title Favourable effects of Bacillus subtilis and Bacillus licheniformis on experimental periodontitis in rats
spellingShingle Favourable effects of Bacillus subtilis and Bacillus licheniformis on experimental periodontitis in rats
Messora, Michel R.
Periodontitis
Probiotics
Rats
Root planing
title_short Favourable effects of Bacillus subtilis and Bacillus licheniformis on experimental periodontitis in rats
title_full Favourable effects of Bacillus subtilis and Bacillus licheniformis on experimental periodontitis in rats
title_fullStr Favourable effects of Bacillus subtilis and Bacillus licheniformis on experimental periodontitis in rats
title_full_unstemmed Favourable effects of Bacillus subtilis and Bacillus licheniformis on experimental periodontitis in rats
title_sort Favourable effects of Bacillus subtilis and Bacillus licheniformis on experimental periodontitis in rats
author Messora, Michel R.
author_facet Messora, Michel R.
Pereira, Luciano J.
Foureaux, Renata
Oliveira, Luiz F.F.
Sordi, Caio G.
Alves, Ana J.N.
Napimoga, Marcelo H.
Nagata, Maria J.H. [UNESP]
Ervolino, Edilson [UNESP]
Furlaneto, Flávia A.C.
author_role author
author2 Pereira, Luciano J.
Foureaux, Renata
Oliveira, Luiz F.F.
Sordi, Caio G.
Alves, Ana J.N.
Napimoga, Marcelo H.
Nagata, Maria J.H. [UNESP]
Ervolino, Edilson [UNESP]
Furlaneto, Flávia A.C.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Federal University of Lavras
Sao Leopoldo Mandic
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Messora, Michel R.
Pereira, Luciano J.
Foureaux, Renata
Oliveira, Luiz F.F.
Sordi, Caio G.
Alves, Ana J.N.
Napimoga, Marcelo H.
Nagata, Maria J.H. [UNESP]
Ervolino, Edilson [UNESP]
Furlaneto, Flávia A.C.
dc.subject.por.fl_str_mv Periodontitis
Probiotics
Rats
Root planing
topic Periodontitis
Probiotics
Rats
Root planing
description Objectives The purposes of this study were to evaluate, in rats: i) the effects of Bacillus species on the development of experimental periodontitis (EP) via microtomographic, immunological and hematological assays (Experiment 1 - E1); ii) the effects of Bacillus species as adjuncts to scaling and root planing (SRP) for the treatment of EP via histomorphometric and immunohistochemical analyses (Experiment 2 - E2). Methods In E1, 24 rats were divided into groups C1 (control), PROB1, EP1 and EP-PROB1. In groups with EP, the mandibular first molar of each animal received a ligature for 14 days. In groups PROB1, animals received Bacillus species for 44 days, starting 30 days before EP induction in Group EP-PROB1. In E2, 24 rats were assigned to groups C2 (control), PROB2, EP-SRP2 and EP-SRP-PROB2. In groups with SRP, EP was induced as described in E1. The ligatures were removed after 14 days and SRP was performed. In groups PROB2, animals received Bacillus species for 15 days, starting after SRP in Group EP-SRP-PROB2. Results In E1, Group EP1 presented bone loss (BL) and eosinophil numbers greater than Group EP-PROB1 (P < 0.05). In Group EP-PROB1, the receptor activator of nuclear factor-kB ligand (RANKL)/osteoprotegerin (OPG) ratio was similar to that of groups without EP. In E2, Group EP-SRP-PROB2 presented fewer TRAP-positive osteoclasts, lower immunolabeling pattern for a proinflammatory cytokine and decreased BL and attachment loss than Group EP-SRP2 (P < 0.05). Conclusions Bacillus species supplementation provided a protective effect against BL and enhanced the effects of SRP in the treatment of EP in rats.
publishDate 2016
dc.date.none.fl_str_mv 2016-06-01
2018-12-11T17:01:31Z
2018-12-11T17:01:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.archoralbio.2016.02.014
Archives of Oral Biology, v. 66, p. 108-119.
1879-1506
0003-9969
http://hdl.handle.net/11449/172631
10.1016/j.archoralbio.2016.02.014
2-s2.0-84959544483
2-s2.0-84959544483.pdf
4408095517346846
0000-0003-4859-0583
url http://dx.doi.org/10.1016/j.archoralbio.2016.02.014
http://hdl.handle.net/11449/172631
identifier_str_mv Archives of Oral Biology, v. 66, p. 108-119.
1879-1506
0003-9969
10.1016/j.archoralbio.2016.02.014
2-s2.0-84959544483
2-s2.0-84959544483.pdf
4408095517346846
0000-0003-4859-0583
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Archives of Oral Biology
0,752
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 108-119
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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