Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in rats
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/omi.12402 http://hdl.handle.net/11449/248034 |
Resumo: | Aim: The aim of this study was to evaluate the effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis (EP) in rats. Methods: Thirty-two rats were divided into four groups: control, C-HD100 (B. bacteriovorus), EP, and EP-HD100. On day 0, EP was induced by the placement of cotton ligatures around the mandibular first molars (MFMs) in the EP and EP-HD100 groups. In the C-HD100 and EP-HD100 groups, suspensions containing 1 × 109 PUF/ml of B. bacteriovorus HD100 were topically administered to the subgingival region of MFMs on days 0, 3, and 7. Animals were euthanized on day 14. Morphometrics analyses were performed in hemimandibles. The levels of tumor necrosis factor alpha (TNF-α), interleukin (IL)-6, monocyte chemoattractant protein (MCP)-1, IL-10, IL-1β, transforming growth factor beta (TGF-β), macrophage colony-stimulating factor (M-CSF) and regulated on activation and normal T cell expressed and secreted (RANTES) were determined by enzymatic immunoassays in gingival tissues. Beta defensin (BD)-1, BD-2, and BD-3, Toll-like receptors (TLR)-2 and TLR-4, and a cluster of differentiation (CD)-4, CD-8 and CD-57 were analyzed by immunohistochemistry in hemimandibles. Data were statistically analyzed. Results: The EP group showed greater alveolar bone loss than EP-HD100 (p <.05). The EP-HD100 group showed higher levels of MCP-1, RANTES, IL-10, and TGF-β, lower levels of TNF-α than the EP group (p <.05). No differences were observed in IL-1β, IL-6, and M-CSF levels between EP and EP-HD100 groups. The C-HD100 group had higher IL-6, TNF-α, RANTES, and MCP-1 levels than the control group (p <.05). Regarding BD, the EP-HD100 group showed a larger immunolabeling pattern for BD-1, BD-2, and BD-3 than the EP group (p <.05). No significant differences in the immunolabeling pattern were observed for TLR-2, TLR-4, CD-4, CD-8, and CD-57 between EP and EP-HD100 groups. Conclusion: The topical use of B. bacteriovorus HD100 reduces alveolar bone loss, increases expression of BD, and modulates the cytokines levels on periodontal tissues in rats with EP. |
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Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in ratsbacteriotherapyBdellovibrioimmunologyperiodontitisratsAim: The aim of this study was to evaluate the effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis (EP) in rats. Methods: Thirty-two rats were divided into four groups: control, C-HD100 (B. bacteriovorus), EP, and EP-HD100. On day 0, EP was induced by the placement of cotton ligatures around the mandibular first molars (MFMs) in the EP and EP-HD100 groups. In the C-HD100 and EP-HD100 groups, suspensions containing 1 × 109 PUF/ml of B. bacteriovorus HD100 were topically administered to the subgingival region of MFMs on days 0, 3, and 7. Animals were euthanized on day 14. Morphometrics analyses were performed in hemimandibles. The levels of tumor necrosis factor alpha (TNF-α), interleukin (IL)-6, monocyte chemoattractant protein (MCP)-1, IL-10, IL-1β, transforming growth factor beta (TGF-β), macrophage colony-stimulating factor (M-CSF) and regulated on activation and normal T cell expressed and secreted (RANTES) were determined by enzymatic immunoassays in gingival tissues. Beta defensin (BD)-1, BD-2, and BD-3, Toll-like receptors (TLR)-2 and TLR-4, and a cluster of differentiation (CD)-4, CD-8 and CD-57 were analyzed by immunohistochemistry in hemimandibles. Data were statistically analyzed. Results: The EP group showed greater alveolar bone loss than EP-HD100 (p <.05). The EP-HD100 group showed higher levels of MCP-1, RANTES, IL-10, and TGF-β, lower levels of TNF-α than the EP group (p <.05). No differences were observed in IL-1β, IL-6, and M-CSF levels between EP and EP-HD100 groups. The C-HD100 group had higher IL-6, TNF-α, RANTES, and MCP-1 levels than the control group (p <.05). Regarding BD, the EP-HD100 group showed a larger immunolabeling pattern for BD-1, BD-2, and BD-3 than the EP group (p <.05). No significant differences in the immunolabeling pattern were observed for TLR-2, TLR-4, CD-4, CD-8, and CD-57 between EP and EP-HD100 groups. Conclusion: The topical use of B. bacteriovorus HD100 reduces alveolar bone loss, increases expression of BD, and modulates the cytokines levels on periodontal tissues in rats with EP.Department of Oral and Maxillofacial Surgery and Periodontology School of Dentistry of Ribeirão Preto University of São Paulo – USP, São PauloDepartment of Prosthodontics and Periodontics School of Dentistry Campinas State UniversityDepartment of Basic Sciences Division of Histology Dental School of Araçatuba São Paulo State UniversityDepartment of Clinical Analyses School of Pharmaceutical Sciences of Ribeirão Preto University of São Paulo – USP, São PauloDepartment of Basic Sciences Division of Histology Dental School of Araçatuba São Paulo State UniversityUniversidade de São Paulo (USP)Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (UNESP)Silva, Pedro Henrique FelixOliveira, Luiz Fernando FerreiraCardoso, Renata SilvaSantana, Sandro IsaiasCasarin, Renato CorreaErvolino, Edilson [UNESP]Salvador, Sergio LuizPalioto, Daniela BazanFurlaneto, Flávia Aparecida ChavesMessora, Michel Reis2023-07-29T13:32:41Z2023-07-29T13:32:41Z2023-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article158-170http://dx.doi.org/10.1111/omi.12402Molecular Oral Microbiology, v. 38, n. 2, p. 158-170, 2023.2041-10142041-1006http://hdl.handle.net/11449/24803410.1111/omi.124022-s2.0-85144023367Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecular Oral Microbiologyinfo:eu-repo/semantics/openAccess2024-09-19T14:03:05Zoai:repositorio.unesp.br:11449/248034Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T14:03:05Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in rats |
title |
Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in rats |
spellingShingle |
Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in rats Silva, Pedro Henrique Felix bacteriotherapy Bdellovibrio immunology periodontitis rats |
title_short |
Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in rats |
title_full |
Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in rats |
title_fullStr |
Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in rats |
title_full_unstemmed |
Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in rats |
title_sort |
Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in rats |
author |
Silva, Pedro Henrique Felix |
author_facet |
Silva, Pedro Henrique Felix Oliveira, Luiz Fernando Ferreira Cardoso, Renata Silva Santana, Sandro Isaias Casarin, Renato Correa Ervolino, Edilson [UNESP] Salvador, Sergio Luiz Palioto, Daniela Bazan Furlaneto, Flávia Aparecida Chaves Messora, Michel Reis |
author_role |
author |
author2 |
Oliveira, Luiz Fernando Ferreira Cardoso, Renata Silva Santana, Sandro Isaias Casarin, Renato Correa Ervolino, Edilson [UNESP] Salvador, Sergio Luiz Palioto, Daniela Bazan Furlaneto, Flávia Aparecida Chaves Messora, Michel Reis |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Silva, Pedro Henrique Felix Oliveira, Luiz Fernando Ferreira Cardoso, Renata Silva Santana, Sandro Isaias Casarin, Renato Correa Ervolino, Edilson [UNESP] Salvador, Sergio Luiz Palioto, Daniela Bazan Furlaneto, Flávia Aparecida Chaves Messora, Michel Reis |
dc.subject.por.fl_str_mv |
bacteriotherapy Bdellovibrio immunology periodontitis rats |
topic |
bacteriotherapy Bdellovibrio immunology periodontitis rats |
description |
Aim: The aim of this study was to evaluate the effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis (EP) in rats. Methods: Thirty-two rats were divided into four groups: control, C-HD100 (B. bacteriovorus), EP, and EP-HD100. On day 0, EP was induced by the placement of cotton ligatures around the mandibular first molars (MFMs) in the EP and EP-HD100 groups. In the C-HD100 and EP-HD100 groups, suspensions containing 1 × 109 PUF/ml of B. bacteriovorus HD100 were topically administered to the subgingival region of MFMs on days 0, 3, and 7. Animals were euthanized on day 14. Morphometrics analyses were performed in hemimandibles. The levels of tumor necrosis factor alpha (TNF-α), interleukin (IL)-6, monocyte chemoattractant protein (MCP)-1, IL-10, IL-1β, transforming growth factor beta (TGF-β), macrophage colony-stimulating factor (M-CSF) and regulated on activation and normal T cell expressed and secreted (RANTES) were determined by enzymatic immunoassays in gingival tissues. Beta defensin (BD)-1, BD-2, and BD-3, Toll-like receptors (TLR)-2 and TLR-4, and a cluster of differentiation (CD)-4, CD-8 and CD-57 were analyzed by immunohistochemistry in hemimandibles. Data were statistically analyzed. Results: The EP group showed greater alveolar bone loss than EP-HD100 (p <.05). The EP-HD100 group showed higher levels of MCP-1, RANTES, IL-10, and TGF-β, lower levels of TNF-α than the EP group (p <.05). No differences were observed in IL-1β, IL-6, and M-CSF levels between EP and EP-HD100 groups. The C-HD100 group had higher IL-6, TNF-α, RANTES, and MCP-1 levels than the control group (p <.05). Regarding BD, the EP-HD100 group showed a larger immunolabeling pattern for BD-1, BD-2, and BD-3 than the EP group (p <.05). No significant differences in the immunolabeling pattern were observed for TLR-2, TLR-4, CD-4, CD-8, and CD-57 between EP and EP-HD100 groups. Conclusion: The topical use of B. bacteriovorus HD100 reduces alveolar bone loss, increases expression of BD, and modulates the cytokines levels on periodontal tissues in rats with EP. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:32:41Z 2023-07-29T13:32:41Z 2023-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/omi.12402 Molecular Oral Microbiology, v. 38, n. 2, p. 158-170, 2023. 2041-1014 2041-1006 http://hdl.handle.net/11449/248034 10.1111/omi.12402 2-s2.0-85144023367 |
url |
http://dx.doi.org/10.1111/omi.12402 http://hdl.handle.net/11449/248034 |
identifier_str_mv |
Molecular Oral Microbiology, v. 38, n. 2, p. 158-170, 2023. 2041-1014 2041-1006 10.1111/omi.12402 2-s2.0-85144023367 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Molecular Oral Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
158-170 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546504651014144 |