Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in rats

Detalhes bibliográficos
Autor(a) principal: Silva, Pedro Henrique Felix
Data de Publicação: 2023
Outros Autores: Oliveira, Luiz Fernando Ferreira, Cardoso, Renata Silva, Santana, Sandro Isaias, Casarin, Renato Correa, Ervolino, Edilson [UNESP], Salvador, Sergio Luiz, Palioto, Daniela Bazan, Furlaneto, Flávia Aparecida Chaves, Messora, Michel Reis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1111/omi.12402
http://hdl.handle.net/11449/248034
Resumo: Aim: The aim of this study was to evaluate the effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis (EP) in rats. Methods: Thirty-two rats were divided into four groups: control, C-HD100 (B. bacteriovorus), EP, and EP-HD100. On day 0, EP was induced by the placement of cotton ligatures around the mandibular first molars (MFMs) in the EP and EP-HD100 groups. In the C-HD100 and EP-HD100 groups, suspensions containing 1 × 109 PUF/ml of B. bacteriovorus HD100 were topically administered to the subgingival region of MFMs on days 0, 3, and 7. Animals were euthanized on day 14. Morphometrics analyses were performed in hemimandibles. The levels of tumor necrosis factor alpha (TNF-α), interleukin (IL)-6, monocyte chemoattractant protein (MCP)-1, IL-10, IL-1β, transforming growth factor beta (TGF-β), macrophage colony-stimulating factor (M-CSF) and regulated on activation and normal T cell expressed and secreted (RANTES) were determined by enzymatic immunoassays in gingival tissues. Beta defensin (BD)-1, BD-2, and BD-3, Toll-like receptors (TLR)-2 and TLR-4, and a cluster of differentiation (CD)-4, CD-8 and CD-57 were analyzed by immunohistochemistry in hemimandibles. Data were statistically analyzed. Results: The EP group showed greater alveolar bone loss than EP-HD100 (p <.05). The EP-HD100 group showed higher levels of MCP-1, RANTES, IL-10, and TGF-β, lower levels of TNF-α than the EP group (p <.05). No differences were observed in IL-1β, IL-6, and M-CSF levels between EP and EP-HD100 groups. The C-HD100 group had higher IL-6, TNF-α, RANTES, and MCP-1 levels than the control group (p <.05). Regarding BD, the EP-HD100 group showed a larger immunolabeling pattern for BD-1, BD-2, and BD-3 than the EP group (p <.05). No significant differences in the immunolabeling pattern were observed for TLR-2, TLR-4, CD-4, CD-8, and CD-57 between EP and EP-HD100 groups. Conclusion: The topical use of B. bacteriovorus HD100 reduces alveolar bone loss, increases expression of BD, and modulates the cytokines levels on periodontal tissues in rats with EP.
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spelling Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in ratsbacteriotherapyBdellovibrioimmunologyperiodontitisratsAim: The aim of this study was to evaluate the effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis (EP) in rats. Methods: Thirty-two rats were divided into four groups: control, C-HD100 (B. bacteriovorus), EP, and EP-HD100. On day 0, EP was induced by the placement of cotton ligatures around the mandibular first molars (MFMs) in the EP and EP-HD100 groups. In the C-HD100 and EP-HD100 groups, suspensions containing 1 × 109 PUF/ml of B. bacteriovorus HD100 were topically administered to the subgingival region of MFMs on days 0, 3, and 7. Animals were euthanized on day 14. Morphometrics analyses were performed in hemimandibles. The levels of tumor necrosis factor alpha (TNF-α), interleukin (IL)-6, monocyte chemoattractant protein (MCP)-1, IL-10, IL-1β, transforming growth factor beta (TGF-β), macrophage colony-stimulating factor (M-CSF) and regulated on activation and normal T cell expressed and secreted (RANTES) were determined by enzymatic immunoassays in gingival tissues. Beta defensin (BD)-1, BD-2, and BD-3, Toll-like receptors (TLR)-2 and TLR-4, and a cluster of differentiation (CD)-4, CD-8 and CD-57 were analyzed by immunohistochemistry in hemimandibles. Data were statistically analyzed. Results: The EP group showed greater alveolar bone loss than EP-HD100 (p <.05). The EP-HD100 group showed higher levels of MCP-1, RANTES, IL-10, and TGF-β, lower levels of TNF-α than the EP group (p <.05). No differences were observed in IL-1β, IL-6, and M-CSF levels between EP and EP-HD100 groups. The C-HD100 group had higher IL-6, TNF-α, RANTES, and MCP-1 levels than the control group (p <.05). Regarding BD, the EP-HD100 group showed a larger immunolabeling pattern for BD-1, BD-2, and BD-3 than the EP group (p <.05). No significant differences in the immunolabeling pattern were observed for TLR-2, TLR-4, CD-4, CD-8, and CD-57 between EP and EP-HD100 groups. Conclusion: The topical use of B. bacteriovorus HD100 reduces alveolar bone loss, increases expression of BD, and modulates the cytokines levels on periodontal tissues in rats with EP.Department of Oral and Maxillofacial Surgery and Periodontology School of Dentistry of Ribeirão Preto University of São Paulo – USP, São PauloDepartment of Prosthodontics and Periodontics School of Dentistry Campinas State UniversityDepartment of Basic Sciences Division of Histology Dental School of Araçatuba São Paulo State UniversityDepartment of Clinical Analyses School of Pharmaceutical Sciences of Ribeirão Preto University of São Paulo – USP, São PauloDepartment of Basic Sciences Division of Histology Dental School of Araçatuba São Paulo State UniversityUniversidade de São Paulo (USP)Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (UNESP)Silva, Pedro Henrique FelixOliveira, Luiz Fernando FerreiraCardoso, Renata SilvaSantana, Sandro IsaiasCasarin, Renato CorreaErvolino, Edilson [UNESP]Salvador, Sergio LuizPalioto, Daniela BazanFurlaneto, Flávia Aparecida ChavesMessora, Michel Reis2023-07-29T13:32:41Z2023-07-29T13:32:41Z2023-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article158-170http://dx.doi.org/10.1111/omi.12402Molecular Oral Microbiology, v. 38, n. 2, p. 158-170, 2023.2041-10142041-1006http://hdl.handle.net/11449/24803410.1111/omi.124022-s2.0-85144023367Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecular Oral Microbiologyinfo:eu-repo/semantics/openAccess2024-09-19T14:03:05Zoai:repositorio.unesp.br:11449/248034Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T14:03:05Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in rats
title Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in rats
spellingShingle Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in rats
Silva, Pedro Henrique Felix
bacteriotherapy
Bdellovibrio
immunology
periodontitis
rats
title_short Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in rats
title_full Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in rats
title_fullStr Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in rats
title_full_unstemmed Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in rats
title_sort Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in rats
author Silva, Pedro Henrique Felix
author_facet Silva, Pedro Henrique Felix
Oliveira, Luiz Fernando Ferreira
Cardoso, Renata Silva
Santana, Sandro Isaias
Casarin, Renato Correa
Ervolino, Edilson [UNESP]
Salvador, Sergio Luiz
Palioto, Daniela Bazan
Furlaneto, Flávia Aparecida Chaves
Messora, Michel Reis
author_role author
author2 Oliveira, Luiz Fernando Ferreira
Cardoso, Renata Silva
Santana, Sandro Isaias
Casarin, Renato Correa
Ervolino, Edilson [UNESP]
Salvador, Sergio Luiz
Palioto, Daniela Bazan
Furlaneto, Flávia Aparecida Chaves
Messora, Michel Reis
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual de Campinas (UNICAMP)
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Silva, Pedro Henrique Felix
Oliveira, Luiz Fernando Ferreira
Cardoso, Renata Silva
Santana, Sandro Isaias
Casarin, Renato Correa
Ervolino, Edilson [UNESP]
Salvador, Sergio Luiz
Palioto, Daniela Bazan
Furlaneto, Flávia Aparecida Chaves
Messora, Michel Reis
dc.subject.por.fl_str_mv bacteriotherapy
Bdellovibrio
immunology
periodontitis
rats
topic bacteriotherapy
Bdellovibrio
immunology
periodontitis
rats
description Aim: The aim of this study was to evaluate the effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis (EP) in rats. Methods: Thirty-two rats were divided into four groups: control, C-HD100 (B. bacteriovorus), EP, and EP-HD100. On day 0, EP was induced by the placement of cotton ligatures around the mandibular first molars (MFMs) in the EP and EP-HD100 groups. In the C-HD100 and EP-HD100 groups, suspensions containing 1 × 109 PUF/ml of B. bacteriovorus HD100 were topically administered to the subgingival region of MFMs on days 0, 3, and 7. Animals were euthanized on day 14. Morphometrics analyses were performed in hemimandibles. The levels of tumor necrosis factor alpha (TNF-α), interleukin (IL)-6, monocyte chemoattractant protein (MCP)-1, IL-10, IL-1β, transforming growth factor beta (TGF-β), macrophage colony-stimulating factor (M-CSF) and regulated on activation and normal T cell expressed and secreted (RANTES) were determined by enzymatic immunoassays in gingival tissues. Beta defensin (BD)-1, BD-2, and BD-3, Toll-like receptors (TLR)-2 and TLR-4, and a cluster of differentiation (CD)-4, CD-8 and CD-57 were analyzed by immunohistochemistry in hemimandibles. Data were statistically analyzed. Results: The EP group showed greater alveolar bone loss than EP-HD100 (p <.05). The EP-HD100 group showed higher levels of MCP-1, RANTES, IL-10, and TGF-β, lower levels of TNF-α than the EP group (p <.05). No differences were observed in IL-1β, IL-6, and M-CSF levels between EP and EP-HD100 groups. The C-HD100 group had higher IL-6, TNF-α, RANTES, and MCP-1 levels than the control group (p <.05). Regarding BD, the EP-HD100 group showed a larger immunolabeling pattern for BD-1, BD-2, and BD-3 than the EP group (p <.05). No significant differences in the immunolabeling pattern were observed for TLR-2, TLR-4, CD-4, CD-8, and CD-57 between EP and EP-HD100 groups. Conclusion: The topical use of B. bacteriovorus HD100 reduces alveolar bone loss, increases expression of BD, and modulates the cytokines levels on periodontal tissues in rats with EP.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T13:32:41Z
2023-07-29T13:32:41Z
2023-04-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1111/omi.12402
Molecular Oral Microbiology, v. 38, n. 2, p. 158-170, 2023.
2041-1014
2041-1006
http://hdl.handle.net/11449/248034
10.1111/omi.12402
2-s2.0-85144023367
url http://dx.doi.org/10.1111/omi.12402
http://hdl.handle.net/11449/248034
identifier_str_mv Molecular Oral Microbiology, v. 38, n. 2, p. 158-170, 2023.
2041-1014
2041-1006
10.1111/omi.12402
2-s2.0-85144023367
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Molecular Oral Microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 158-170
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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