Potential Use of Brazilian Green Propolis Extracts as New Photosensitizers for Antimicrobial Photodynamic Therapy against Cariogenic Microorganisms
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/pathogens12020155 http://hdl.handle.net/11449/248414 |
Resumo: | The synergic effect of Streptococcus mutans and Candida albicans increases dental caries severity. Antimicrobial photodynamic therapy (aPDT) is a non-invasive treatment for antimicrobial aspects. However, the current photosensitizers (PS) have many downsides for dental applications. This study aimed to evaluate the efficiency of two different Brazilian green propolis (BGP-AF and BGP-AG) as PS for aPDT against these microorganisms. A single-species biofilm was irradiated with crude extracts and their fractions and controls. Such extracts showed the best results and were evaluated in dual-species biofilms. Photodegradation, reactive oxygen species (ROS), cytotoxicity, and color stability assays were also investigated. Reductions higher than 3 log10 CFU/mL (p < 0.0001) occurred for crude BGP in single- and dual-species biofilms. Singlet oxygen was produced in BGP (p < 0.0001). BGP-mediated aPDT delayed S. mutans and C. albicans regrowth after 24 h of treatment (p < 0.0001). Both BGP did not change the color of dental materials (p > 0.05). BGP-AF-mediated aPDT showed 72.41% of oral keratinocyte viability (p < 0.0001). BGP extracts may be used in aPDT against S. mutans and C. albicans. Specifically, BGP-AF may represent a promising PS for dental applications. |
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Potential Use of Brazilian Green Propolis Extracts as New Photosensitizers for Antimicrobial Photodynamic Therapy against Cariogenic Microorganismsantimicrobial photodynamic therapyBrazilian green propoliscariogenic microorganismsnatural photosensitizerThe synergic effect of Streptococcus mutans and Candida albicans increases dental caries severity. Antimicrobial photodynamic therapy (aPDT) is a non-invasive treatment for antimicrobial aspects. However, the current photosensitizers (PS) have many downsides for dental applications. This study aimed to evaluate the efficiency of two different Brazilian green propolis (BGP-AF and BGP-AG) as PS for aPDT against these microorganisms. A single-species biofilm was irradiated with crude extracts and their fractions and controls. Such extracts showed the best results and were evaluated in dual-species biofilms. Photodegradation, reactive oxygen species (ROS), cytotoxicity, and color stability assays were also investigated. Reductions higher than 3 log10 CFU/mL (p < 0.0001) occurred for crude BGP in single- and dual-species biofilms. Singlet oxygen was produced in BGP (p < 0.0001). BGP-mediated aPDT delayed S. mutans and C. albicans regrowth after 24 h of treatment (p < 0.0001). Both BGP did not change the color of dental materials (p > 0.05). BGP-AF-mediated aPDT showed 72.41% of oral keratinocyte viability (p < 0.0001). BGP extracts may be used in aPDT against S. mutans and C. albicans. Specifically, BGP-AF may represent a promising PS for dental applications.Department of Morphology and Pediatric Dentistry School of Dentistry São Paulo State University (UNESP), SPDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP), SPDepartment of Clinical Analysis School of Pharmaceutical Sciences São Paulo State University (UNESP), SPDepartment of Morphology and Pediatric Dentistry School of Dentistry São Paulo State University (UNESP), SPDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP), SPDepartment of Clinical Analysis School of Pharmaceutical Sciences São Paulo State University (UNESP), SPUniversidade Estadual Paulista (UNESP)de Oliveira, Analú Barros [UNESP]Ferrisse, Túlio Morandin [UNESP]França, Gabriela Gomes [UNESP]de Annunzio, Sarah Raquel [UNESP]Kopp, Willian [UNESP]Fontana, Carla Raquel [UNESP]Brighenti, Fernanda Lourenção [UNESP]2023-07-29T13:43:27Z2023-07-29T13:43:27Z2023-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/pathogens12020155Pathogens, v. 12, n. 2, 2023.2076-0817http://hdl.handle.net/11449/24841410.3390/pathogens120201552-s2.0-85148759659Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPathogensinfo:eu-repo/semantics/openAccess2024-06-21T15:18:56Zoai:repositorio.unesp.br:11449/248414Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:08:01.454228Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Potential Use of Brazilian Green Propolis Extracts as New Photosensitizers for Antimicrobial Photodynamic Therapy against Cariogenic Microorganisms |
title |
Potential Use of Brazilian Green Propolis Extracts as New Photosensitizers for Antimicrobial Photodynamic Therapy against Cariogenic Microorganisms |
spellingShingle |
Potential Use of Brazilian Green Propolis Extracts as New Photosensitizers for Antimicrobial Photodynamic Therapy against Cariogenic Microorganisms de Oliveira, Analú Barros [UNESP] antimicrobial photodynamic therapy Brazilian green propolis cariogenic microorganisms natural photosensitizer |
title_short |
Potential Use of Brazilian Green Propolis Extracts as New Photosensitizers for Antimicrobial Photodynamic Therapy against Cariogenic Microorganisms |
title_full |
Potential Use of Brazilian Green Propolis Extracts as New Photosensitizers for Antimicrobial Photodynamic Therapy against Cariogenic Microorganisms |
title_fullStr |
Potential Use of Brazilian Green Propolis Extracts as New Photosensitizers for Antimicrobial Photodynamic Therapy against Cariogenic Microorganisms |
title_full_unstemmed |
Potential Use of Brazilian Green Propolis Extracts as New Photosensitizers for Antimicrobial Photodynamic Therapy against Cariogenic Microorganisms |
title_sort |
Potential Use of Brazilian Green Propolis Extracts as New Photosensitizers for Antimicrobial Photodynamic Therapy against Cariogenic Microorganisms |
author |
de Oliveira, Analú Barros [UNESP] |
author_facet |
de Oliveira, Analú Barros [UNESP] Ferrisse, Túlio Morandin [UNESP] França, Gabriela Gomes [UNESP] de Annunzio, Sarah Raquel [UNESP] Kopp, Willian [UNESP] Fontana, Carla Raquel [UNESP] Brighenti, Fernanda Lourenção [UNESP] |
author_role |
author |
author2 |
Ferrisse, Túlio Morandin [UNESP] França, Gabriela Gomes [UNESP] de Annunzio, Sarah Raquel [UNESP] Kopp, Willian [UNESP] Fontana, Carla Raquel [UNESP] Brighenti, Fernanda Lourenção [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
de Oliveira, Analú Barros [UNESP] Ferrisse, Túlio Morandin [UNESP] França, Gabriela Gomes [UNESP] de Annunzio, Sarah Raquel [UNESP] Kopp, Willian [UNESP] Fontana, Carla Raquel [UNESP] Brighenti, Fernanda Lourenção [UNESP] |
dc.subject.por.fl_str_mv |
antimicrobial photodynamic therapy Brazilian green propolis cariogenic microorganisms natural photosensitizer |
topic |
antimicrobial photodynamic therapy Brazilian green propolis cariogenic microorganisms natural photosensitizer |
description |
The synergic effect of Streptococcus mutans and Candida albicans increases dental caries severity. Antimicrobial photodynamic therapy (aPDT) is a non-invasive treatment for antimicrobial aspects. However, the current photosensitizers (PS) have many downsides for dental applications. This study aimed to evaluate the efficiency of two different Brazilian green propolis (BGP-AF and BGP-AG) as PS for aPDT against these microorganisms. A single-species biofilm was irradiated with crude extracts and their fractions and controls. Such extracts showed the best results and were evaluated in dual-species biofilms. Photodegradation, reactive oxygen species (ROS), cytotoxicity, and color stability assays were also investigated. Reductions higher than 3 log10 CFU/mL (p < 0.0001) occurred for crude BGP in single- and dual-species biofilms. Singlet oxygen was produced in BGP (p < 0.0001). BGP-mediated aPDT delayed S. mutans and C. albicans regrowth after 24 h of treatment (p < 0.0001). Both BGP did not change the color of dental materials (p > 0.05). BGP-AF-mediated aPDT showed 72.41% of oral keratinocyte viability (p < 0.0001). BGP extracts may be used in aPDT against S. mutans and C. albicans. Specifically, BGP-AF may represent a promising PS for dental applications. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:43:27Z 2023-07-29T13:43:27Z 2023-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/pathogens12020155 Pathogens, v. 12, n. 2, 2023. 2076-0817 http://hdl.handle.net/11449/248414 10.3390/pathogens12020155 2-s2.0-85148759659 |
url |
http://dx.doi.org/10.3390/pathogens12020155 http://hdl.handle.net/11449/248414 |
identifier_str_mv |
Pathogens, v. 12, n. 2, 2023. 2076-0817 10.3390/pathogens12020155 2-s2.0-85148759659 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pathogens |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128899193765888 |