Cost of transport, but not gluteus medius and red blood cells monocarboxylate-transporters density differentiated Brazilian Sport Horses at two performance levels
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.rvsc.2021.12.008 http://hdl.handle.net/11449/230107 |
Resumo: | Cost of transport (COT) and monocarboxylate transporters (MCTs) could affect the ability to perform fast actions during a jumping discipline. This study aimed to compare the COT and evaluate the MCT1, MCT4, and their auxiliary protein CD147 content in the gluteus medius and RBCs of Brazilian sport horses (BH), a breed developed for jumping competitions, with low-level (LL) or intermediate-level (IL) jumping capacities. The physiological difference between the horses was assessed by an incremental jump test (IJT), in which the cost of lactate (COTLAC) and heart rate (COTHR) of running were determined for each animal by the ratio between each variable and the running speed. Western blotting was performed on muscle and RBC membranes to quantify MCT1, MCT4, and CD147. IL showed lower COTLAC and COTHR than LL at all jumping heights. The amount of MCT1, MCT4, and CD147 found in muscle and RBCs were not dependent on performance level. Muscle MCT4 and MCT1 were correlated positively with CD147. We conclude that the relatively small differences between performances did not relevantly influence MCT expression in BH. While MCT analyses are inaccessible for most trainers and veterinarians, the cost of transport measurements is a feasible and sensitive tool to distinguish intermediate and low-level jumping horses. |
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Repositório Institucional da UNESP |
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Cost of transport, but not gluteus medius and red blood cells monocarboxylate-transporters density differentiated Brazilian Sport Horses at two performance levelsEnduranceHeart rateJumping horseLactateTrack-and-fieldCost of transport (COT) and monocarboxylate transporters (MCTs) could affect the ability to perform fast actions during a jumping discipline. This study aimed to compare the COT and evaluate the MCT1, MCT4, and their auxiliary protein CD147 content in the gluteus medius and RBCs of Brazilian sport horses (BH), a breed developed for jumping competitions, with low-level (LL) or intermediate-level (IL) jumping capacities. The physiological difference between the horses was assessed by an incremental jump test (IJT), in which the cost of lactate (COTLAC) and heart rate (COTHR) of running were determined for each animal by the ratio between each variable and the running speed. Western blotting was performed on muscle and RBC membranes to quantify MCT1, MCT4, and CD147. IL showed lower COTLAC and COTHR than LL at all jumping heights. The amount of MCT1, MCT4, and CD147 found in muscle and RBCs were not dependent on performance level. Muscle MCT4 and MCT1 were correlated positively with CD147. We conclude that the relatively small differences between performances did not relevantly influence MCT expression in BH. While MCT analyses are inaccessible for most trainers and veterinarians, the cost of transport measurements is a feasible and sensitive tool to distinguish intermediate and low-level jumping horses.Department of Animal Morphology and Physiology Laboratory of Pharmacology and Equine Exercise Physiology (LAFEQ) São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences (FCAV), JaboticabalDepartment of Technology São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences (FCAV), JaboticabalTeaching and Research Division Army Central Hospital Brazilian Army, Rod. Presidente Dutra, Km. 306Faculdades Metropolitanas Unidas HOVETDepartment of Animal Morphology and Physiology Laboratory of Pharmacology and Equine Exercise Physiology (LAFEQ) São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences (FCAV), JaboticabalDepartment of Technology São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences (FCAV), JaboticabalUniversidade Estadual Paulista (UNESP)Brazilian ArmyHOVETFeringer-Júnior, Walter Heinz [UNESP]de Carvalho, Júlia Ribeiro Garcia [UNESP]Moranza, Henriette Gellert [UNESP]de Almeida, Maria Luiza Mendes [UNESP]Lemos, Eliana Gertrudes Macedo [UNESP]Soares, Otávio Augusto BrioschiRibeiro, Gesianede Camargo Ferraz, Guilherme [UNESP]2022-04-29T08:37:59Z2022-04-29T08:37:59Z2022-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article20-27http://dx.doi.org/10.1016/j.rvsc.2021.12.008Research in Veterinary Science, v. 143, p. 20-27.1532-26610034-5288http://hdl.handle.net/11449/23010710.1016/j.rvsc.2021.12.0082-s2.0-85121629031Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengResearch in Veterinary Scienceinfo:eu-repo/semantics/openAccess2022-04-29T08:37:59Zoai:repositorio.unesp.br:11449/230107Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T08:37:59Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cost of transport, but not gluteus medius and red blood cells monocarboxylate-transporters density differentiated Brazilian Sport Horses at two performance levels |
title |
Cost of transport, but not gluteus medius and red blood cells monocarboxylate-transporters density differentiated Brazilian Sport Horses at two performance levels |
spellingShingle |
Cost of transport, but not gluteus medius and red blood cells monocarboxylate-transporters density differentiated Brazilian Sport Horses at two performance levels Feringer-Júnior, Walter Heinz [UNESP] Endurance Heart rate Jumping horse Lactate Track-and-field |
title_short |
Cost of transport, but not gluteus medius and red blood cells monocarboxylate-transporters density differentiated Brazilian Sport Horses at two performance levels |
title_full |
Cost of transport, but not gluteus medius and red blood cells monocarboxylate-transporters density differentiated Brazilian Sport Horses at two performance levels |
title_fullStr |
Cost of transport, but not gluteus medius and red blood cells monocarboxylate-transporters density differentiated Brazilian Sport Horses at two performance levels |
title_full_unstemmed |
Cost of transport, but not gluteus medius and red blood cells monocarboxylate-transporters density differentiated Brazilian Sport Horses at two performance levels |
title_sort |
Cost of transport, but not gluteus medius and red blood cells monocarboxylate-transporters density differentiated Brazilian Sport Horses at two performance levels |
author |
Feringer-Júnior, Walter Heinz [UNESP] |
author_facet |
Feringer-Júnior, Walter Heinz [UNESP] de Carvalho, Júlia Ribeiro Garcia [UNESP] Moranza, Henriette Gellert [UNESP] de Almeida, Maria Luiza Mendes [UNESP] Lemos, Eliana Gertrudes Macedo [UNESP] Soares, Otávio Augusto Brioschi Ribeiro, Gesiane de Camargo Ferraz, Guilherme [UNESP] |
author_role |
author |
author2 |
de Carvalho, Júlia Ribeiro Garcia [UNESP] Moranza, Henriette Gellert [UNESP] de Almeida, Maria Luiza Mendes [UNESP] Lemos, Eliana Gertrudes Macedo [UNESP] Soares, Otávio Augusto Brioschi Ribeiro, Gesiane de Camargo Ferraz, Guilherme [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Brazilian Army HOVET |
dc.contributor.author.fl_str_mv |
Feringer-Júnior, Walter Heinz [UNESP] de Carvalho, Júlia Ribeiro Garcia [UNESP] Moranza, Henriette Gellert [UNESP] de Almeida, Maria Luiza Mendes [UNESP] Lemos, Eliana Gertrudes Macedo [UNESP] Soares, Otávio Augusto Brioschi Ribeiro, Gesiane de Camargo Ferraz, Guilherme [UNESP] |
dc.subject.por.fl_str_mv |
Endurance Heart rate Jumping horse Lactate Track-and-field |
topic |
Endurance Heart rate Jumping horse Lactate Track-and-field |
description |
Cost of transport (COT) and monocarboxylate transporters (MCTs) could affect the ability to perform fast actions during a jumping discipline. This study aimed to compare the COT and evaluate the MCT1, MCT4, and their auxiliary protein CD147 content in the gluteus medius and RBCs of Brazilian sport horses (BH), a breed developed for jumping competitions, with low-level (LL) or intermediate-level (IL) jumping capacities. The physiological difference between the horses was assessed by an incremental jump test (IJT), in which the cost of lactate (COTLAC) and heart rate (COTHR) of running were determined for each animal by the ratio between each variable and the running speed. Western blotting was performed on muscle and RBC membranes to quantify MCT1, MCT4, and CD147. IL showed lower COTLAC and COTHR than LL at all jumping heights. The amount of MCT1, MCT4, and CD147 found in muscle and RBCs were not dependent on performance level. Muscle MCT4 and MCT1 were correlated positively with CD147. We conclude that the relatively small differences between performances did not relevantly influence MCT expression in BH. While MCT analyses are inaccessible for most trainers and veterinarians, the cost of transport measurements is a feasible and sensitive tool to distinguish intermediate and low-level jumping horses. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-29T08:37:59Z 2022-04-29T08:37:59Z 2022-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.rvsc.2021.12.008 Research in Veterinary Science, v. 143, p. 20-27. 1532-2661 0034-5288 http://hdl.handle.net/11449/230107 10.1016/j.rvsc.2021.12.008 2-s2.0-85121629031 |
url |
http://dx.doi.org/10.1016/j.rvsc.2021.12.008 http://hdl.handle.net/11449/230107 |
identifier_str_mv |
Research in Veterinary Science, v. 143, p. 20-27. 1532-2661 0034-5288 10.1016/j.rvsc.2021.12.008 2-s2.0-85121629031 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Research in Veterinary Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
20-27 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965675323654144 |