Caffeine consumption attenuates ethanol-induced inflammation through the regulation of adenosinergic receptors in the UChB rats cerebellum
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1093/toxres/tfab067 http://hdl.handle.net/11449/233544 |
Resumo: | Caffeine consumption is able to interfere in cellular processes related to inflammatory mechanisms by acting through the adenosinergic system. This study aimed to recognize alterations related to adenosinergic system and inflammatory process in the cerebellum of University of Chile Bibulous (UChB) rats after the consumption of ethanol and caffeine. UChB and Wistar rats, males at 5 months old, were divided into the groups (n = 15/group): (i) Control (Wistar rats receiving water); (ii) Ethanol group (UChB rats receiving ethanol solution at 10%) and (iii) Ethanol+caffeine group (UChB rats receiving ethanol solution at 10% added of 3 g/L of caffeine). The cerebellar tissue was collected and processed for immunohistochemistry, Reverse transcription polymerase chain reaction (RT-PCR) and western blotting techniques for the adenosinergic receptors A1 and A2a and inflammatory markers, including Nuclear factor kappa B (NFkB), TLR4, TLR2, MyD88, TNF-α, COX-2, iNOS and microglial marker Iba-1. Results showed ethanol and caffeine consumption differentially altering the immunolocalization of adenosinergic receptors and inflammatory markers in the cerebellar tissue. The A2a receptor was overexpressed in the Ethanol group and was evident in the glial cells. The Ethanol group had increased protein levels for NFκB and TLR4, expressively in Bergmann glia and Purkinje cells. Caffeine reduced the expression of these markers to levels similar to those found in the Control group. The A1 gene was upregulated the Ethanol group, but not its protein levels, suggesting post-Transcriptional interference. In conclusion, caffeine seems to attenuate ethanol-induced inflammation in the cerebellum of UChB rats through the A1 and A2a modulation, playing a neuroprotective role in the chronic context of ethanol consumption. |
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Caffeine consumption attenuates ethanol-induced inflammation through the regulation of adenosinergic receptors in the UChB rats cerebellumadenosinergic receptorcaffeinecerebellumethanolinflammationUChB ratsCaffeine consumption is able to interfere in cellular processes related to inflammatory mechanisms by acting through the adenosinergic system. This study aimed to recognize alterations related to adenosinergic system and inflammatory process in the cerebellum of University of Chile Bibulous (UChB) rats after the consumption of ethanol and caffeine. UChB and Wistar rats, males at 5 months old, were divided into the groups (n = 15/group): (i) Control (Wistar rats receiving water); (ii) Ethanol group (UChB rats receiving ethanol solution at 10%) and (iii) Ethanol+caffeine group (UChB rats receiving ethanol solution at 10% added of 3 g/L of caffeine). The cerebellar tissue was collected and processed for immunohistochemistry, Reverse transcription polymerase chain reaction (RT-PCR) and western blotting techniques for the adenosinergic receptors A1 and A2a and inflammatory markers, including Nuclear factor kappa B (NFkB), TLR4, TLR2, MyD88, TNF-α, COX-2, iNOS and microglial marker Iba-1. Results showed ethanol and caffeine consumption differentially altering the immunolocalization of adenosinergic receptors and inflammatory markers in the cerebellar tissue. The A2a receptor was overexpressed in the Ethanol group and was evident in the glial cells. The Ethanol group had increased protein levels for NFκB and TLR4, expressively in Bergmann glia and Purkinje cells. Caffeine reduced the expression of these markers to levels similar to those found in the Control group. The A1 gene was upregulated the Ethanol group, but not its protein levels, suggesting post-Transcriptional interference. In conclusion, caffeine seems to attenuate ethanol-induced inflammation in the cerebellum of UChB rats through the A1 and A2a modulation, playing a neuroprotective role in the chronic context of ethanol consumption.Department of Structural and Functional Biology University of Campinas (UNICAMP), 255 Monteiro Lobato StDepartment of Food and Nutrition University of Campinas (UNICAMP), 80 Monteiro Lobato StDepartment of Surgery and Anatomy University of São Paulo (USP), 3900 Bandeirantes AveDepartment of Structural and Functional Biology State University of São Paulo (UNESP), 250 Prof. Dr. Antônio Celso Wagner Zanin StDepartment of Morphology and Pathology Federal University of São Carlos (UFSCar), 13571 Biblioteca Comunitária AveDepartment of Structural and Functional Biology State University of São Paulo (UNESP), 250 Prof. Dr. Antônio Celso Wagner Zanin StUniversidade Estadual de Campinas (UNICAMP)Universidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Universidade Federal de São Carlos (UFSCar)Rossetto, Isabela Maria UrraCagnon, Valéria Helena AlvesKido, Larissa AkemiLizarte Neto, Fermino SanchesTirapelli, Luís FernandoTirapelli, Daniela Pretti Da CunhaDe Almeida Chuffa, Luiz Gustavo [UNESP]Martinez, Francisco Eduardo [UNESP]Martinez, Marcelo2022-05-01T09:30:31Z2022-05-01T09:30:31Z2021-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article835-849http://dx.doi.org/10.1093/toxres/tfab067Toxicology Research, v. 10, n. 4, p. 835-849, 2021.2045-45382045-452Xhttp://hdl.handle.net/11449/23354410.1093/toxres/tfab0672-s2.0-85115016020Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengToxicology Researchinfo:eu-repo/semantics/openAccess2022-05-01T09:30:31Zoai:repositorio.unesp.br:11449/233544Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:55:54.569484Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Caffeine consumption attenuates ethanol-induced inflammation through the regulation of adenosinergic receptors in the UChB rats cerebellum |
title |
Caffeine consumption attenuates ethanol-induced inflammation through the regulation of adenosinergic receptors in the UChB rats cerebellum |
spellingShingle |
Caffeine consumption attenuates ethanol-induced inflammation through the regulation of adenosinergic receptors in the UChB rats cerebellum Rossetto, Isabela Maria Urra adenosinergic receptor caffeine cerebellum ethanol inflammation UChB rats |
title_short |
Caffeine consumption attenuates ethanol-induced inflammation through the regulation of adenosinergic receptors in the UChB rats cerebellum |
title_full |
Caffeine consumption attenuates ethanol-induced inflammation through the regulation of adenosinergic receptors in the UChB rats cerebellum |
title_fullStr |
Caffeine consumption attenuates ethanol-induced inflammation through the regulation of adenosinergic receptors in the UChB rats cerebellum |
title_full_unstemmed |
Caffeine consumption attenuates ethanol-induced inflammation through the regulation of adenosinergic receptors in the UChB rats cerebellum |
title_sort |
Caffeine consumption attenuates ethanol-induced inflammation through the regulation of adenosinergic receptors in the UChB rats cerebellum |
author |
Rossetto, Isabela Maria Urra |
author_facet |
Rossetto, Isabela Maria Urra Cagnon, Valéria Helena Alves Kido, Larissa Akemi Lizarte Neto, Fermino Sanches Tirapelli, Luís Fernando Tirapelli, Daniela Pretti Da Cunha De Almeida Chuffa, Luiz Gustavo [UNESP] Martinez, Francisco Eduardo [UNESP] Martinez, Marcelo |
author_role |
author |
author2 |
Cagnon, Valéria Helena Alves Kido, Larissa Akemi Lizarte Neto, Fermino Sanches Tirapelli, Luís Fernando Tirapelli, Daniela Pretti Da Cunha De Almeida Chuffa, Luiz Gustavo [UNESP] Martinez, Francisco Eduardo [UNESP] Martinez, Marcelo |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Rossetto, Isabela Maria Urra Cagnon, Valéria Helena Alves Kido, Larissa Akemi Lizarte Neto, Fermino Sanches Tirapelli, Luís Fernando Tirapelli, Daniela Pretti Da Cunha De Almeida Chuffa, Luiz Gustavo [UNESP] Martinez, Francisco Eduardo [UNESP] Martinez, Marcelo |
dc.subject.por.fl_str_mv |
adenosinergic receptor caffeine cerebellum ethanol inflammation UChB rats |
topic |
adenosinergic receptor caffeine cerebellum ethanol inflammation UChB rats |
description |
Caffeine consumption is able to interfere in cellular processes related to inflammatory mechanisms by acting through the adenosinergic system. This study aimed to recognize alterations related to adenosinergic system and inflammatory process in the cerebellum of University of Chile Bibulous (UChB) rats after the consumption of ethanol and caffeine. UChB and Wistar rats, males at 5 months old, were divided into the groups (n = 15/group): (i) Control (Wistar rats receiving water); (ii) Ethanol group (UChB rats receiving ethanol solution at 10%) and (iii) Ethanol+caffeine group (UChB rats receiving ethanol solution at 10% added of 3 g/L of caffeine). The cerebellar tissue was collected and processed for immunohistochemistry, Reverse transcription polymerase chain reaction (RT-PCR) and western blotting techniques for the adenosinergic receptors A1 and A2a and inflammatory markers, including Nuclear factor kappa B (NFkB), TLR4, TLR2, MyD88, TNF-α, COX-2, iNOS and microglial marker Iba-1. Results showed ethanol and caffeine consumption differentially altering the immunolocalization of adenosinergic receptors and inflammatory markers in the cerebellar tissue. The A2a receptor was overexpressed in the Ethanol group and was evident in the glial cells. The Ethanol group had increased protein levels for NFκB and TLR4, expressively in Bergmann glia and Purkinje cells. Caffeine reduced the expression of these markers to levels similar to those found in the Control group. The A1 gene was upregulated the Ethanol group, but not its protein levels, suggesting post-Transcriptional interference. In conclusion, caffeine seems to attenuate ethanol-induced inflammation in the cerebellum of UChB rats through the A1 and A2a modulation, playing a neuroprotective role in the chronic context of ethanol consumption. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-01 2022-05-01T09:30:31Z 2022-05-01T09:30:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1093/toxres/tfab067 Toxicology Research, v. 10, n. 4, p. 835-849, 2021. 2045-4538 2045-452X http://hdl.handle.net/11449/233544 10.1093/toxres/tfab067 2-s2.0-85115016020 |
url |
http://dx.doi.org/10.1093/toxres/tfab067 http://hdl.handle.net/11449/233544 |
identifier_str_mv |
Toxicology Research, v. 10, n. 4, p. 835-849, 2021. 2045-4538 2045-452X 10.1093/toxres/tfab067 2-s2.0-85115016020 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Toxicology Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
835-849 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128437140848640 |