Caffeine consumption attenuates ethanol-induced inflammation through the regulation of adenosinergic receptors in the UChB rats cerebellum

Detalhes bibliográficos
Autor(a) principal: Rossetto, Isabela Maria Urra
Data de Publicação: 2021
Outros Autores: Cagnon, Valéria Helena Alves, Kido, Larissa Akemi, Lizarte Neto, Fermino Sanches, Tirapelli, Luís Fernando, Tirapelli, Daniela Pretti Da Cunha, De Almeida Chuffa, Luiz Gustavo [UNESP], Martinez, Francisco Eduardo [UNESP], Martinez, Marcelo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1093/toxres/tfab067
http://hdl.handle.net/11449/233544
Resumo: Caffeine consumption is able to interfere in cellular processes related to inflammatory mechanisms by acting through the adenosinergic system. This study aimed to recognize alterations related to adenosinergic system and inflammatory process in the cerebellum of University of Chile Bibulous (UChB) rats after the consumption of ethanol and caffeine. UChB and Wistar rats, males at 5 months old, were divided into the groups (n = 15/group): (i) Control (Wistar rats receiving water); (ii) Ethanol group (UChB rats receiving ethanol solution at 10%) and (iii) Ethanol+caffeine group (UChB rats receiving ethanol solution at 10% added of 3 g/L of caffeine). The cerebellar tissue was collected and processed for immunohistochemistry, Reverse transcription polymerase chain reaction (RT-PCR) and western blotting techniques for the adenosinergic receptors A1 and A2a and inflammatory markers, including Nuclear factor kappa B (NFkB), TLR4, TLR2, MyD88, TNF-α, COX-2, iNOS and microglial marker Iba-1. Results showed ethanol and caffeine consumption differentially altering the immunolocalization of adenosinergic receptors and inflammatory markers in the cerebellar tissue. The A2a receptor was overexpressed in the Ethanol group and was evident in the glial cells. The Ethanol group had increased protein levels for NFκB and TLR4, expressively in Bergmann glia and Purkinje cells. Caffeine reduced the expression of these markers to levels similar to those found in the Control group. The A1 gene was upregulated the Ethanol group, but not its protein levels, suggesting post-Transcriptional interference. In conclusion, caffeine seems to attenuate ethanol-induced inflammation in the cerebellum of UChB rats through the A1 and A2a modulation, playing a neuroprotective role in the chronic context of ethanol consumption.
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spelling Caffeine consumption attenuates ethanol-induced inflammation through the regulation of adenosinergic receptors in the UChB rats cerebellumadenosinergic receptorcaffeinecerebellumethanolinflammationUChB ratsCaffeine consumption is able to interfere in cellular processes related to inflammatory mechanisms by acting through the adenosinergic system. This study aimed to recognize alterations related to adenosinergic system and inflammatory process in the cerebellum of University of Chile Bibulous (UChB) rats after the consumption of ethanol and caffeine. UChB and Wistar rats, males at 5 months old, were divided into the groups (n = 15/group): (i) Control (Wistar rats receiving water); (ii) Ethanol group (UChB rats receiving ethanol solution at 10%) and (iii) Ethanol+caffeine group (UChB rats receiving ethanol solution at 10% added of 3 g/L of caffeine). The cerebellar tissue was collected and processed for immunohistochemistry, Reverse transcription polymerase chain reaction (RT-PCR) and western blotting techniques for the adenosinergic receptors A1 and A2a and inflammatory markers, including Nuclear factor kappa B (NFkB), TLR4, TLR2, MyD88, TNF-α, COX-2, iNOS and microglial marker Iba-1. Results showed ethanol and caffeine consumption differentially altering the immunolocalization of adenosinergic receptors and inflammatory markers in the cerebellar tissue. The A2a receptor was overexpressed in the Ethanol group and was evident in the glial cells. The Ethanol group had increased protein levels for NFκB and TLR4, expressively in Bergmann glia and Purkinje cells. Caffeine reduced the expression of these markers to levels similar to those found in the Control group. The A1 gene was upregulated the Ethanol group, but not its protein levels, suggesting post-Transcriptional interference. In conclusion, caffeine seems to attenuate ethanol-induced inflammation in the cerebellum of UChB rats through the A1 and A2a modulation, playing a neuroprotective role in the chronic context of ethanol consumption.Department of Structural and Functional Biology University of Campinas (UNICAMP), 255 Monteiro Lobato StDepartment of Food and Nutrition University of Campinas (UNICAMP), 80 Monteiro Lobato StDepartment of Surgery and Anatomy University of São Paulo (USP), 3900 Bandeirantes AveDepartment of Structural and Functional Biology State University of São Paulo (UNESP), 250 Prof. Dr. Antônio Celso Wagner Zanin StDepartment of Morphology and Pathology Federal University of São Carlos (UFSCar), 13571 Biblioteca Comunitária AveDepartment of Structural and Functional Biology State University of São Paulo (UNESP), 250 Prof. Dr. Antônio Celso Wagner Zanin StUniversidade Estadual de Campinas (UNICAMP)Universidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Universidade Federal de São Carlos (UFSCar)Rossetto, Isabela Maria UrraCagnon, Valéria Helena AlvesKido, Larissa AkemiLizarte Neto, Fermino SanchesTirapelli, Luís FernandoTirapelli, Daniela Pretti Da CunhaDe Almeida Chuffa, Luiz Gustavo [UNESP]Martinez, Francisco Eduardo [UNESP]Martinez, Marcelo2022-05-01T09:30:31Z2022-05-01T09:30:31Z2021-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article835-849http://dx.doi.org/10.1093/toxres/tfab067Toxicology Research, v. 10, n. 4, p. 835-849, 2021.2045-45382045-452Xhttp://hdl.handle.net/11449/23354410.1093/toxres/tfab0672-s2.0-85115016020Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengToxicology Researchinfo:eu-repo/semantics/openAccess2022-05-01T09:30:31Zoai:repositorio.unesp.br:11449/233544Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:55:54.569484Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Caffeine consumption attenuates ethanol-induced inflammation through the regulation of adenosinergic receptors in the UChB rats cerebellum
title Caffeine consumption attenuates ethanol-induced inflammation through the regulation of adenosinergic receptors in the UChB rats cerebellum
spellingShingle Caffeine consumption attenuates ethanol-induced inflammation through the regulation of adenosinergic receptors in the UChB rats cerebellum
Rossetto, Isabela Maria Urra
adenosinergic receptor
caffeine
cerebellum
ethanol
inflammation
UChB rats
title_short Caffeine consumption attenuates ethanol-induced inflammation through the regulation of adenosinergic receptors in the UChB rats cerebellum
title_full Caffeine consumption attenuates ethanol-induced inflammation through the regulation of adenosinergic receptors in the UChB rats cerebellum
title_fullStr Caffeine consumption attenuates ethanol-induced inflammation through the regulation of adenosinergic receptors in the UChB rats cerebellum
title_full_unstemmed Caffeine consumption attenuates ethanol-induced inflammation through the regulation of adenosinergic receptors in the UChB rats cerebellum
title_sort Caffeine consumption attenuates ethanol-induced inflammation through the regulation of adenosinergic receptors in the UChB rats cerebellum
author Rossetto, Isabela Maria Urra
author_facet Rossetto, Isabela Maria Urra
Cagnon, Valéria Helena Alves
Kido, Larissa Akemi
Lizarte Neto, Fermino Sanches
Tirapelli, Luís Fernando
Tirapelli, Daniela Pretti Da Cunha
De Almeida Chuffa, Luiz Gustavo [UNESP]
Martinez, Francisco Eduardo [UNESP]
Martinez, Marcelo
author_role author
author2 Cagnon, Valéria Helena Alves
Kido, Larissa Akemi
Lizarte Neto, Fermino Sanches
Tirapelli, Luís Fernando
Tirapelli, Daniela Pretti Da Cunha
De Almeida Chuffa, Luiz Gustavo [UNESP]
Martinez, Francisco Eduardo [UNESP]
Martinez, Marcelo
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Campinas (UNICAMP)
Universidade de São Paulo (USP)
Universidade Estadual Paulista (UNESP)
Universidade Federal de São Carlos (UFSCar)
dc.contributor.author.fl_str_mv Rossetto, Isabela Maria Urra
Cagnon, Valéria Helena Alves
Kido, Larissa Akemi
Lizarte Neto, Fermino Sanches
Tirapelli, Luís Fernando
Tirapelli, Daniela Pretti Da Cunha
De Almeida Chuffa, Luiz Gustavo [UNESP]
Martinez, Francisco Eduardo [UNESP]
Martinez, Marcelo
dc.subject.por.fl_str_mv adenosinergic receptor
caffeine
cerebellum
ethanol
inflammation
UChB rats
topic adenosinergic receptor
caffeine
cerebellum
ethanol
inflammation
UChB rats
description Caffeine consumption is able to interfere in cellular processes related to inflammatory mechanisms by acting through the adenosinergic system. This study aimed to recognize alterations related to adenosinergic system and inflammatory process in the cerebellum of University of Chile Bibulous (UChB) rats after the consumption of ethanol and caffeine. UChB and Wistar rats, males at 5 months old, were divided into the groups (n = 15/group): (i) Control (Wistar rats receiving water); (ii) Ethanol group (UChB rats receiving ethanol solution at 10%) and (iii) Ethanol+caffeine group (UChB rats receiving ethanol solution at 10% added of 3 g/L of caffeine). The cerebellar tissue was collected and processed for immunohistochemistry, Reverse transcription polymerase chain reaction (RT-PCR) and western blotting techniques for the adenosinergic receptors A1 and A2a and inflammatory markers, including Nuclear factor kappa B (NFkB), TLR4, TLR2, MyD88, TNF-α, COX-2, iNOS and microglial marker Iba-1. Results showed ethanol and caffeine consumption differentially altering the immunolocalization of adenosinergic receptors and inflammatory markers in the cerebellar tissue. The A2a receptor was overexpressed in the Ethanol group and was evident in the glial cells. The Ethanol group had increased protein levels for NFκB and TLR4, expressively in Bergmann glia and Purkinje cells. Caffeine reduced the expression of these markers to levels similar to those found in the Control group. The A1 gene was upregulated the Ethanol group, but not its protein levels, suggesting post-Transcriptional interference. In conclusion, caffeine seems to attenuate ethanol-induced inflammation in the cerebellum of UChB rats through the A1 and A2a modulation, playing a neuroprotective role in the chronic context of ethanol consumption.
publishDate 2021
dc.date.none.fl_str_mv 2021-08-01
2022-05-01T09:30:31Z
2022-05-01T09:30:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1093/toxres/tfab067
Toxicology Research, v. 10, n. 4, p. 835-849, 2021.
2045-4538
2045-452X
http://hdl.handle.net/11449/233544
10.1093/toxres/tfab067
2-s2.0-85115016020
url http://dx.doi.org/10.1093/toxres/tfab067
http://hdl.handle.net/11449/233544
identifier_str_mv Toxicology Research, v. 10, n. 4, p. 835-849, 2021.
2045-4538
2045-452X
10.1093/toxres/tfab067
2-s2.0-85115016020
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Toxicology Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 835-849
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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