Inflammasomes in placental explants of women with preeclampsia cultured with monosodium urate may be modulated by vitamin D

Detalhes bibliográficos
Autor(a) principal: Nunes, Priscila Rezeck [UNESP]
Data de Publicação: 2022
Outros Autores: Romao-Veiga, Mariana [UNESP], Ribeiro, Vanessa Rocha [UNESP], de Oliveira, Larissa Ragozo Cardoso [UNESP], de Carvalho Depra, Igor [UNESP], de Oliveira, Leandro Gustavo [UNESP], Peracoli, Jose Carlos [UNESP], Peracoli, Maria Terezinha Serrao [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1080/10641955.2022.2063330
http://hdl.handle.net/11449/241777
Resumo: Objectives: Preeclampsia (PE) is an important syndrome of gestation characterized by placental and systemic inflammation. High plasma concentration of uric acid are frequently associated with inflammation and endothelial dysfunction and may contribute to PE pathogenesis. This study aimed to evaluate the vitamin D (VD) immunomodulatory effect on the NLRP1/NLRP3 inflammasomes in placental explants from preeclamptic (PE) and normotensive (NT) pregnant women. Study design: Placental explants from 10 late-onset PE (LOPE), 10 early-onset PE (EOPE), and 10 NT pregnant women were cultured with or without monosodium urate (MSU) and VD. Main outcome measures: Gene and protein expression of NLRP1, NLRP3, HMGB1, caspase-1, interleukin-1 beta (IL-1β), and IL-18 were determined by quantitative PCR and Western blotting/ELISA. Statistical significance was accepted at p < 0.05. Results: Basal gene and protein expression of NLRP1/NLRP3 and IL-1β, IL-18 and HMGB1 were significantly higher in explants from EOPE compared to LOPE and NT pregnant women. In addition, culture with MSU increased these inflammatory markers, and concomitant treatment with MSU+VD decreased this effect. Conclusions: The results demonstrated that NLRP1 and NLRP3 inflammasomes are upregulated in the placental tissue of EOPE women, associated with high production of inflammatory cytokines. The in vitro treatment with VD downregulated placental inflammasomes induced by MSU, suggesting its immunomodulatory role in the systemic inflammation of PE.
id UNSP_74e9712479dce6a38c2869fc1f40bc0b
oai_identifier_str oai:repositorio.unesp.br:11449/241777
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Inflammasomes in placental explants of women with preeclampsia cultured with monosodium urate may be modulated by vitamin DcytokinesinflammasomeMSUplacental explantsPreeclampsiavitamin DObjectives: Preeclampsia (PE) is an important syndrome of gestation characterized by placental and systemic inflammation. High plasma concentration of uric acid are frequently associated with inflammation and endothelial dysfunction and may contribute to PE pathogenesis. This study aimed to evaluate the vitamin D (VD) immunomodulatory effect on the NLRP1/NLRP3 inflammasomes in placental explants from preeclamptic (PE) and normotensive (NT) pregnant women. Study design: Placental explants from 10 late-onset PE (LOPE), 10 early-onset PE (EOPE), and 10 NT pregnant women were cultured with or without monosodium urate (MSU) and VD. Main outcome measures: Gene and protein expression of NLRP1, NLRP3, HMGB1, caspase-1, interleukin-1 beta (IL-1β), and IL-18 were determined by quantitative PCR and Western blotting/ELISA. Statistical significance was accepted at p < 0.05. Results: Basal gene and protein expression of NLRP1/NLRP3 and IL-1β, IL-18 and HMGB1 were significantly higher in explants from EOPE compared to LOPE and NT pregnant women. In addition, culture with MSU increased these inflammatory markers, and concomitant treatment with MSU+VD decreased this effect. Conclusions: The results demonstrated that NLRP1 and NLRP3 inflammasomes are upregulated in the placental tissue of EOPE women, associated with high production of inflammatory cytokines. The in vitro treatment with VD downregulated placental inflammasomes induced by MSU, suggesting its immunomodulatory role in the systemic inflammation of PE.Botucatu Medical School Sao Paulo State University (Unesp)Institute of Biosciences Sao Paulo State University (unesp)Experimental Research Unit Sao Paulo State University (Unesp)Botucatu Medical School Sao Paulo State University (Unesp)Institute of Biosciences Sao Paulo State University (unesp)Experimental Research Unit Sao Paulo State University (Unesp)Universidade Estadual Paulista (UNESP)Nunes, Priscila Rezeck [UNESP]Romao-Veiga, Mariana [UNESP]Ribeiro, Vanessa Rocha [UNESP]de Oliveira, Larissa Ragozo Cardoso [UNESP]de Carvalho Depra, Igor [UNESP]de Oliveira, Leandro Gustavo [UNESP]Peracoli, Jose Carlos [UNESP]Peracoli, Maria Terezinha Serrao [UNESP]2023-03-02T00:08:20Z2023-03-02T00:08:20Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article139-148http://dx.doi.org/10.1080/10641955.2022.2063330Hypertension in Pregnancy, v. 41, n. 2, p. 139-148, 2022.1525-60651064-1955http://hdl.handle.net/11449/24177710.1080/10641955.2022.20633302-s2.0-85128935898Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengHypertension in Pregnancyinfo:eu-repo/semantics/openAccess2024-08-16T14:13:01Zoai:repositorio.unesp.br:11449/241777Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T14:13:01Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Inflammasomes in placental explants of women with preeclampsia cultured with monosodium urate may be modulated by vitamin D
title Inflammasomes in placental explants of women with preeclampsia cultured with monosodium urate may be modulated by vitamin D
spellingShingle Inflammasomes in placental explants of women with preeclampsia cultured with monosodium urate may be modulated by vitamin D
Nunes, Priscila Rezeck [UNESP]
cytokines
inflammasome
MSU
placental explants
Preeclampsia
vitamin D
title_short Inflammasomes in placental explants of women with preeclampsia cultured with monosodium urate may be modulated by vitamin D
title_full Inflammasomes in placental explants of women with preeclampsia cultured with monosodium urate may be modulated by vitamin D
title_fullStr Inflammasomes in placental explants of women with preeclampsia cultured with monosodium urate may be modulated by vitamin D
title_full_unstemmed Inflammasomes in placental explants of women with preeclampsia cultured with monosodium urate may be modulated by vitamin D
title_sort Inflammasomes in placental explants of women with preeclampsia cultured with monosodium urate may be modulated by vitamin D
author Nunes, Priscila Rezeck [UNESP]
author_facet Nunes, Priscila Rezeck [UNESP]
Romao-Veiga, Mariana [UNESP]
Ribeiro, Vanessa Rocha [UNESP]
de Oliveira, Larissa Ragozo Cardoso [UNESP]
de Carvalho Depra, Igor [UNESP]
de Oliveira, Leandro Gustavo [UNESP]
Peracoli, Jose Carlos [UNESP]
Peracoli, Maria Terezinha Serrao [UNESP]
author_role author
author2 Romao-Veiga, Mariana [UNESP]
Ribeiro, Vanessa Rocha [UNESP]
de Oliveira, Larissa Ragozo Cardoso [UNESP]
de Carvalho Depra, Igor [UNESP]
de Oliveira, Leandro Gustavo [UNESP]
Peracoli, Jose Carlos [UNESP]
Peracoli, Maria Terezinha Serrao [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Nunes, Priscila Rezeck [UNESP]
Romao-Veiga, Mariana [UNESP]
Ribeiro, Vanessa Rocha [UNESP]
de Oliveira, Larissa Ragozo Cardoso [UNESP]
de Carvalho Depra, Igor [UNESP]
de Oliveira, Leandro Gustavo [UNESP]
Peracoli, Jose Carlos [UNESP]
Peracoli, Maria Terezinha Serrao [UNESP]
dc.subject.por.fl_str_mv cytokines
inflammasome
MSU
placental explants
Preeclampsia
vitamin D
topic cytokines
inflammasome
MSU
placental explants
Preeclampsia
vitamin D
description Objectives: Preeclampsia (PE) is an important syndrome of gestation characterized by placental and systemic inflammation. High plasma concentration of uric acid are frequently associated with inflammation and endothelial dysfunction and may contribute to PE pathogenesis. This study aimed to evaluate the vitamin D (VD) immunomodulatory effect on the NLRP1/NLRP3 inflammasomes in placental explants from preeclamptic (PE) and normotensive (NT) pregnant women. Study design: Placental explants from 10 late-onset PE (LOPE), 10 early-onset PE (EOPE), and 10 NT pregnant women were cultured with or without monosodium urate (MSU) and VD. Main outcome measures: Gene and protein expression of NLRP1, NLRP3, HMGB1, caspase-1, interleukin-1 beta (IL-1β), and IL-18 were determined by quantitative PCR and Western blotting/ELISA. Statistical significance was accepted at p < 0.05. Results: Basal gene and protein expression of NLRP1/NLRP3 and IL-1β, IL-18 and HMGB1 were significantly higher in explants from EOPE compared to LOPE and NT pregnant women. In addition, culture with MSU increased these inflammatory markers, and concomitant treatment with MSU+VD decreased this effect. Conclusions: The results demonstrated that NLRP1 and NLRP3 inflammasomes are upregulated in the placental tissue of EOPE women, associated with high production of inflammatory cytokines. The in vitro treatment with VD downregulated placental inflammasomes induced by MSU, suggesting its immunomodulatory role in the systemic inflammation of PE.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
2023-03-02T00:08:20Z
2023-03-02T00:08:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1080/10641955.2022.2063330
Hypertension in Pregnancy, v. 41, n. 2, p. 139-148, 2022.
1525-6065
1064-1955
http://hdl.handle.net/11449/241777
10.1080/10641955.2022.2063330
2-s2.0-85128935898
url http://dx.doi.org/10.1080/10641955.2022.2063330
http://hdl.handle.net/11449/241777
identifier_str_mv Hypertension in Pregnancy, v. 41, n. 2, p. 139-148, 2022.
1525-6065
1064-1955
10.1080/10641955.2022.2063330
2-s2.0-85128935898
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Hypertension in Pregnancy
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 139-148
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128207452372992