Inflammasomes in placental explants of women with preeclampsia cultured with monosodium urate may be modulated by vitamin D
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/10641955.2022.2063330 http://hdl.handle.net/11449/241777 |
Resumo: | Objectives: Preeclampsia (PE) is an important syndrome of gestation characterized by placental and systemic inflammation. High plasma concentration of uric acid are frequently associated with inflammation and endothelial dysfunction and may contribute to PE pathogenesis. This study aimed to evaluate the vitamin D (VD) immunomodulatory effect on the NLRP1/NLRP3 inflammasomes in placental explants from preeclamptic (PE) and normotensive (NT) pregnant women. Study design: Placental explants from 10 late-onset PE (LOPE), 10 early-onset PE (EOPE), and 10 NT pregnant women were cultured with or without monosodium urate (MSU) and VD. Main outcome measures: Gene and protein expression of NLRP1, NLRP3, HMGB1, caspase-1, interleukin-1 beta (IL-1β), and IL-18 were determined by quantitative PCR and Western blotting/ELISA. Statistical significance was accepted at p < 0.05. Results: Basal gene and protein expression of NLRP1/NLRP3 and IL-1β, IL-18 and HMGB1 were significantly higher in explants from EOPE compared to LOPE and NT pregnant women. In addition, culture with MSU increased these inflammatory markers, and concomitant treatment with MSU+VD decreased this effect. Conclusions: The results demonstrated that NLRP1 and NLRP3 inflammasomes are upregulated in the placental tissue of EOPE women, associated with high production of inflammatory cytokines. The in vitro treatment with VD downregulated placental inflammasomes induced by MSU, suggesting its immunomodulatory role in the systemic inflammation of PE. |
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Repositório Institucional da UNESP |
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Inflammasomes in placental explants of women with preeclampsia cultured with monosodium urate may be modulated by vitamin DcytokinesinflammasomeMSUplacental explantsPreeclampsiavitamin DObjectives: Preeclampsia (PE) is an important syndrome of gestation characterized by placental and systemic inflammation. High plasma concentration of uric acid are frequently associated with inflammation and endothelial dysfunction and may contribute to PE pathogenesis. This study aimed to evaluate the vitamin D (VD) immunomodulatory effect on the NLRP1/NLRP3 inflammasomes in placental explants from preeclamptic (PE) and normotensive (NT) pregnant women. Study design: Placental explants from 10 late-onset PE (LOPE), 10 early-onset PE (EOPE), and 10 NT pregnant women were cultured with or without monosodium urate (MSU) and VD. Main outcome measures: Gene and protein expression of NLRP1, NLRP3, HMGB1, caspase-1, interleukin-1 beta (IL-1β), and IL-18 were determined by quantitative PCR and Western blotting/ELISA. Statistical significance was accepted at p < 0.05. Results: Basal gene and protein expression of NLRP1/NLRP3 and IL-1β, IL-18 and HMGB1 were significantly higher in explants from EOPE compared to LOPE and NT pregnant women. In addition, culture with MSU increased these inflammatory markers, and concomitant treatment with MSU+VD decreased this effect. Conclusions: The results demonstrated that NLRP1 and NLRP3 inflammasomes are upregulated in the placental tissue of EOPE women, associated with high production of inflammatory cytokines. The in vitro treatment with VD downregulated placental inflammasomes induced by MSU, suggesting its immunomodulatory role in the systemic inflammation of PE.Botucatu Medical School Sao Paulo State University (Unesp)Institute of Biosciences Sao Paulo State University (unesp)Experimental Research Unit Sao Paulo State University (Unesp)Botucatu Medical School Sao Paulo State University (Unesp)Institute of Biosciences Sao Paulo State University (unesp)Experimental Research Unit Sao Paulo State University (Unesp)Universidade Estadual Paulista (UNESP)Nunes, Priscila Rezeck [UNESP]Romao-Veiga, Mariana [UNESP]Ribeiro, Vanessa Rocha [UNESP]de Oliveira, Larissa Ragozo Cardoso [UNESP]de Carvalho Depra, Igor [UNESP]de Oliveira, Leandro Gustavo [UNESP]Peracoli, Jose Carlos [UNESP]Peracoli, Maria Terezinha Serrao [UNESP]2023-03-02T00:08:20Z2023-03-02T00:08:20Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article139-148http://dx.doi.org/10.1080/10641955.2022.2063330Hypertension in Pregnancy, v. 41, n. 2, p. 139-148, 2022.1525-60651064-1955http://hdl.handle.net/11449/24177710.1080/10641955.2022.20633302-s2.0-85128935898Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengHypertension in Pregnancyinfo:eu-repo/semantics/openAccess2024-08-16T14:13:01Zoai:repositorio.unesp.br:11449/241777Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T14:13:01Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Inflammasomes in placental explants of women with preeclampsia cultured with monosodium urate may be modulated by vitamin D |
title |
Inflammasomes in placental explants of women with preeclampsia cultured with monosodium urate may be modulated by vitamin D |
spellingShingle |
Inflammasomes in placental explants of women with preeclampsia cultured with monosodium urate may be modulated by vitamin D Nunes, Priscila Rezeck [UNESP] cytokines inflammasome MSU placental explants Preeclampsia vitamin D |
title_short |
Inflammasomes in placental explants of women with preeclampsia cultured with monosodium urate may be modulated by vitamin D |
title_full |
Inflammasomes in placental explants of women with preeclampsia cultured with monosodium urate may be modulated by vitamin D |
title_fullStr |
Inflammasomes in placental explants of women with preeclampsia cultured with monosodium urate may be modulated by vitamin D |
title_full_unstemmed |
Inflammasomes in placental explants of women with preeclampsia cultured with monosodium urate may be modulated by vitamin D |
title_sort |
Inflammasomes in placental explants of women with preeclampsia cultured with monosodium urate may be modulated by vitamin D |
author |
Nunes, Priscila Rezeck [UNESP] |
author_facet |
Nunes, Priscila Rezeck [UNESP] Romao-Veiga, Mariana [UNESP] Ribeiro, Vanessa Rocha [UNESP] de Oliveira, Larissa Ragozo Cardoso [UNESP] de Carvalho Depra, Igor [UNESP] de Oliveira, Leandro Gustavo [UNESP] Peracoli, Jose Carlos [UNESP] Peracoli, Maria Terezinha Serrao [UNESP] |
author_role |
author |
author2 |
Romao-Veiga, Mariana [UNESP] Ribeiro, Vanessa Rocha [UNESP] de Oliveira, Larissa Ragozo Cardoso [UNESP] de Carvalho Depra, Igor [UNESP] de Oliveira, Leandro Gustavo [UNESP] Peracoli, Jose Carlos [UNESP] Peracoli, Maria Terezinha Serrao [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Nunes, Priscila Rezeck [UNESP] Romao-Veiga, Mariana [UNESP] Ribeiro, Vanessa Rocha [UNESP] de Oliveira, Larissa Ragozo Cardoso [UNESP] de Carvalho Depra, Igor [UNESP] de Oliveira, Leandro Gustavo [UNESP] Peracoli, Jose Carlos [UNESP] Peracoli, Maria Terezinha Serrao [UNESP] |
dc.subject.por.fl_str_mv |
cytokines inflammasome MSU placental explants Preeclampsia vitamin D |
topic |
cytokines inflammasome MSU placental explants Preeclampsia vitamin D |
description |
Objectives: Preeclampsia (PE) is an important syndrome of gestation characterized by placental and systemic inflammation. High plasma concentration of uric acid are frequently associated with inflammation and endothelial dysfunction and may contribute to PE pathogenesis. This study aimed to evaluate the vitamin D (VD) immunomodulatory effect on the NLRP1/NLRP3 inflammasomes in placental explants from preeclamptic (PE) and normotensive (NT) pregnant women. Study design: Placental explants from 10 late-onset PE (LOPE), 10 early-onset PE (EOPE), and 10 NT pregnant women were cultured with or without monosodium urate (MSU) and VD. Main outcome measures: Gene and protein expression of NLRP1, NLRP3, HMGB1, caspase-1, interleukin-1 beta (IL-1β), and IL-18 were determined by quantitative PCR and Western blotting/ELISA. Statistical significance was accepted at p < 0.05. Results: Basal gene and protein expression of NLRP1/NLRP3 and IL-1β, IL-18 and HMGB1 were significantly higher in explants from EOPE compared to LOPE and NT pregnant women. In addition, culture with MSU increased these inflammatory markers, and concomitant treatment with MSU+VD decreased this effect. Conclusions: The results demonstrated that NLRP1 and NLRP3 inflammasomes are upregulated in the placental tissue of EOPE women, associated with high production of inflammatory cytokines. The in vitro treatment with VD downregulated placental inflammasomes induced by MSU, suggesting its immunomodulatory role in the systemic inflammation of PE. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 2023-03-02T00:08:20Z 2023-03-02T00:08:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/10641955.2022.2063330 Hypertension in Pregnancy, v. 41, n. 2, p. 139-148, 2022. 1525-6065 1064-1955 http://hdl.handle.net/11449/241777 10.1080/10641955.2022.2063330 2-s2.0-85128935898 |
url |
http://dx.doi.org/10.1080/10641955.2022.2063330 http://hdl.handle.net/11449/241777 |
identifier_str_mv |
Hypertension in Pregnancy, v. 41, n. 2, p. 139-148, 2022. 1525-6065 1064-1955 10.1080/10641955.2022.2063330 2-s2.0-85128935898 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Hypertension in Pregnancy |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
139-148 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128207452372992 |